##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512995_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1597116 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.454796645954332 31.0 31.0 34.0 27.0 34.0 2 30.62009772615139 31.0 31.0 34.0 27.0 34.0 3 30.71930968069946 31.0 31.0 34.0 27.0 34.0 4 34.19558378978108 37.0 35.0 37.0 30.0 37.0 5 33.839774944337165 37.0 35.0 37.0 29.0 37.0 6 33.69961856245883 37.0 35.0 37.0 29.0 37.0 7 33.571758720092966 37.0 35.0 37.0 28.0 37.0 8 33.45305976522682 37.0 35.0 37.0 28.0 37.0 9 34.91960634042862 39.0 35.0 39.0 28.0 39.0 10 34.81643975766319 39.0 35.0 39.0 27.0 39.0 11 34.8326758983067 39.0 35.0 39.0 27.0 39.0 12 34.68158731112831 39.0 35.0 39.0 27.0 39.0 13 34.6622236581438 39.0 35.0 39.0 27.0 39.0 14 35.7192445633254 40.0 36.0 41.0 27.0 41.0 15 35.71785393171191 40.0 36.0 41.0 27.0 41.0 16 35.69630070702441 40.0 36.0 41.0 27.0 41.0 17 35.67872840795534 40.0 36.0 41.0 27.0 41.0 18 35.60292239261268 40.0 36.0 41.0 27.0 41.0 19 35.59159885693963 40.0 36.0 41.0 27.0 41.0 20 35.56356019224653 40.0 36.0 41.0 27.0 41.0 21 35.509269833875564 40.0 36.0 41.0 26.0 41.0 22 35.46303524603097 40.0 36.0 41.0 26.0 41.0 23 35.35894574971386 40.0 36.0 41.0 26.0 41.0 24 35.33520232719477 40.0 36.0 41.0 25.0 41.0 25 35.23131444428582 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6617870556777667 1101 2 0.6730398615642414 1101 3 0.7612000096245595 1101 4 0.5905512040502785 1101 5 0.8781576249542198 1101 6 0.7977913257762452 1101 7 0.8264421542207216 1101 8 0.8647763530470556 1101 9 0.7043086715243447 1101 10 1.126312546546302 1101 11 1.0768685682673862 1101 12 1.2512462360348025 1101 13 1.2198357166221 1101 14 1.2669910441233867 1101 15 1.3045836680622998 1101 16 1.5412303504771643 1101 17 1.4746838336222368 1101 18 0.9689072825948983 1101 19 1.2835780517726647 1101 20 1.3319238111680463 1101 21 1.4770996633397075 1101 22 1.3600962466750488 1101 23 1.375461571669156 1101 24 1.220170107791425 1101 25 1.2069937949732363 1102 1 0.6677127720372269 1102 2 0.5986898707842307 1102 3 0.5346087195249751 1102 4 0.5649061626782128 1102 5 0.6125761383552017 1102 6 0.643439448898036 1102 7 0.5596501967455012 1102 8 0.6965731057585316 1102 9 0.7284948496242194 1102 10 0.9291610600230626 1102 11 0.6979251705793672 1102 12 0.7024647480885662 1102 13 0.8414864398649726 1102 14 1.017884793644491 1102 15 0.9428685985587393 1102 16 0.8731630875003447 1102 17 0.8605995637653692 1102 18 0.9139382979640729 1102 19 0.9910061632242275 1102 20 0.886260026695183 1102 21 0.9407814112138482 1102 22 0.978840357880749 1102 23 1.0172719901724534 1102 24 0.960030688086583 1102 25 1.095860342579961 1103 1 0.39216432391261336 1103 2 0.2753629717470858 1103 3 0.29370834319631456 1103 4 0.2635554510221638 1103 5 0.44579018784941127 1103 6 0.3971293583355262 1103 7 0.2791524912629413 1103 8 0.3071907573140038 1103 9 0.3446939056549212 1103 10 0.5419395559020828 1103 11 0.4640477426953993 1103 12 0.5127354218419526 1103 13 0.5025743182886586 1103 14 0.5855018884330434 1103 15 0.4344478462775072 1103 16 0.4928010173084658 1103 17 0.6818894807245286 1103 18 0.6986541056481101 1103 19 0.708941825167777 1103 20 0.7440818661844304 1103 21 0.2746916237581942 1103 22 0.6163200105432125 1103 23 0.6640297834786537 1103 24 0.695543342883731 1103 25 0.8093721193041574 1104 1 0.3303300195631955 1104 2 0.4588984474228255 1104 3 0.3502466403150244 1104 4 0.3975195173199637 1104 5 0.42621352643477906 1104 6 0.5766982027653142 1104 7 0.5312878716691785 1104 8 0.3598918919982097 1104 9 0.46086602588992065 1104 10 0.5718639510018164 1104 11 0.49988226689752224 1104 12 0.49253584444769416 1104 13 0.537688214200621 1104 14 0.7135055037885323 1104 15 0.6594363040108249 1104 16 0.6552140228169989 1104 17 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-2.334163684972779 2216 15 -2.432760192003933 2216 16 -2.19695030491215 2216 17 -2.2688017165179133 2216 18 -2.096693075655935 2216 19 -2.2243724554869146 2216 20 -2.7023939679121014 2216 21 -2.617327740080597 2216 22 -2.9692823808871793 2216 23 -2.1572799487270373 2216 24 -2.228257535454837 2216 25 -1.9897143601993434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39635.0 3 0.0 4 668.0 5 5809.0 6 6839.0 7 5341.0 8 4439.0 9 3901.0 10 3319.0 11 3005.0 12 2764.0 13 2628.0 14 2564.0 15 2562.0 16 2720.0 17 2700.0 18 3069.0 19 3676.0 20 4594.0 21 5989.0 22 7550.0 23 9293.0 24 11113.0 25 12659.0 26 14422.0 27 16074.0 28 18358.0 29 21933.0 30 27443.0 31 35081.0 32 44978.0 33 57920.0 34 75550.0 35 99531.0 36 135593.0 37 211577.0 38 691819.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.443245881772775 19.527499650665803 13.970610811627097 27.05864365593433 2 14.060611828585811 20.46009239165144 39.402071486443674 26.077224293319073 3 16.892386025811525 24.088607214504144 30.407371787647236 28.6116349720371 4 11.612196915322302 17.060612551403988 37.28062099399624 34.04656953927747 5 12.980474619260715 37.86953922808266 35.93078583986957 13.219200312787049 6 32.36617716721933 37.350712694151824 17.339133137333043 12.943977001295808 7 27.878156221246936 31.62049369616972 22.021472294651844 18.479877787931496 8 25.06575460219562 36.71105441686726 20.26419629017484 17.958994690762278 9 25.80738919989902 15.018125031711756 20.042081894950613 39.13240387343861 10 14.340765794423804 29.220397031695715 34.709215119321996 21.72962205455848 11 34.29829455267463 22.600229546935964 24.253080193029813 18.848395707359593 12 22.508614843392387 25.012696053046017 31.4808889178329 20.997800185728696 13 28.34186058306727 21.81408445765502 26.04391757986822 23.800137379409495 14 20.772023947056493 21.767626389851618 27.761278328489947 29.699071334601946 15 22.924230223503496 30.47991824783879 23.4495204198815 23.14633110877621 16 22.494922870846477 28.405371267613393 26.828658867645206 22.27104699389492 17 21.654439349206925 28.614615913591674 27.558937256763645 22.172007480437752 18 21.63746278422521 27.883013327238977 28.875621828245478 21.603902060290338 19 23.11004610926468 27.349982092273628 27.63994950772537 21.90002229073632 20 23.248652520637975 27.27324527129919 28.014609381261522 21.463492826801314 21 22.904737604994004 26.894869812129997 27.36928774696875 22.831104835907247 22 22.90129772225513 27.66829502863304 27.380712363487458 22.049694885624373 23 22.37451756791617 27.335960568925486 27.592548067892373 22.69697379526597 24 22.751509596046873 26.95702754214472 28.123129440817074 22.16833342099134 25 22.623411297523322 27.18630131153659 27.81289095324027 22.37739643769982 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 91.0 1 91.0 2 233.5 3 376.0 4 376.0 5 376.0 6 873.0 7 1370.0 8 1370.0 9 1370.0 10 2044.5 11 2719.0 12 2719.0 13 2719.0 14 4772.5 15 6826.0 16 6826.0 17 6826.0 18 12768.0 19 18710.0 20 18710.0 21 18710.0 22 29647.5 23 40585.0 24 40585.0 25 40585.0 26 57380.5 27 74176.0 28 74176.0 29 74176.0 30 94392.5 31 114609.0 32 114609.0 33 114609.0 34 132301.0 35 149993.0 36 149993.0 37 149993.0 38 163584.5 39 177176.0 40 177176.0 41 177176.0 42 186796.5 43 196417.0 44 196417.0 45 196417.0 46 201020.5 47 205624.0 48 205624.0 49 205624.0 50 199781.5 51 193939.0 52 193939.0 53 193939.0 54 175644.0 55 157349.0 56 157349.0 57 157349.0 58 136133.0 59 114917.0 60 114917.0 61 114917.0 62 94172.5 63 73428.0 64 73428.0 65 73428.0 66 56713.5 67 39999.0 68 39999.0 69 39999.0 70 28941.5 71 17884.0 72 17884.0 73 17884.0 74 12521.5 75 7159.0 76 7159.0 77 7159.0 78 4915.5 79 2672.0 80 2672.0 81 2672.0 82 1748.0 83 824.0 84 824.0 85 824.0 86 515.0 87 206.0 88 206.0 89 206.0 90 131.5 91 57.0 92 57.0 93 57.0 94 33.0 95 9.0 96 9.0 97 9.0 98 5.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07657552738811708 2 1.2522571935914484E-4 3 0.0 4 0.028175786855807593 5 0.07150388575407171 6 0.07513543161548691 7 0.03424923424472612 8 0.0644912454699596 9 0.04414206607409856 10 0.10813240866662159 11 0.003568933001735629 12 0.010205896127770306 13 0.004946415914686222 14 0.005822995950200236 15 0.005885608809879809 16 0.015590602060213534 17 0.026172175346061276 18 6.887414564752968E-4 19 0.002504514387182897 20 0.0037567715807743456 21 6.261285967957242E-5 22 6.261285967957243E-4 23 0.0 24 0.0 25 0.0036315458614152004 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1597116.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.66465212476126 #Duplication Level Percentage of deduplicated Percentage of total 1 83.227042503802 62.14118175919094 2 11.746560469926996 17.541057022991424 3 2.854111634669554 6.393037569835077 4 0.9502188997669673 2.83791054373896 5 0.42985002291371555 1.6047301213336616 6 0.22971411940323846 1.0290914888033202 7 0.13010371610707333 0.6799904092291329 8 0.08719881781856957 0.5208535518491143 9 0.06066036598704857 0.40762666117653124 >10 0.2580910206201385 3.316864318858121 >50 0.015199280714808597 0.7881188549837479 >100 0.009822232867277306 1.4539115356494352 >500 7.554258013825547E-4 0.36605939428427026 >1k 6.714896012289373E-4 0.9195667680762023 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3610 0.22603242344325647 No Hit TATCAACGCAGAGTACTTTTTTTTT 2522 0.15790963211188166 No Hit GGTATCAACGCAGAGTACTTTTTTT 2169 0.1358072926449926 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 6.261285967957243E-5 0.0 8 0.0 0.0 0.0 6.261285967957243E-5 0.0 9 0.0 0.0 0.0 6.261285967957243E-5 0.0 10 0.0 0.0 0.0 6.261285967957243E-5 0.0 11 0.0 0.0 0.0 6.261285967957243E-5 0.0 12 0.0 0.0 0.0 6.261285967957243E-5 0.0 13 0.0 0.0 0.0 6.261285967957243E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTTTAC 60 2.54463E-5 14.264025 1 GGTATCA 780 0.0 12.435302 1 TAGGACC 375 0.0 12.43218 4 GCCTTAG 135 4.654794E-9 11.27034 1 CAACGCT 60 0.005809788 11.100161 4 GTCCTAC 755 0.0 10.831841 1 TGGCGAG 865 0.0 10.751672 18 GTACCGT 90 9.3901705E-5 10.56926 6 ACTAGCC 135 5.6221324E-8 10.563624 3 TCTACAC 190 9.094947E-12 10.508026 3 GTCCTAA 390 0.0 10.484667 1 GTAGGAC 795 0.0 10.404172 3 GGCGAGA 510 0.0 10.23431 19 GGCGAGG 400 0.0 10.201747 19 TCCAACG 75 0.002670879 10.122663 18 GTATCAA 1545 0.0 10.032518 1 CTAGGAC 190 9.822543E-11 10.007644 3 TGTACCG 105 4.0365325E-5 9.967491 5 CCAATAC 125 2.5441896E-6 9.887551 3 GCTAGAC 125 2.5441896E-6 9.887551 3 >>END_MODULE