FastQCFastQC Report
Thu 26 May 2016
SRR1512994_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512994_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757915
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18030.23788947309394853No Hit
GTCCTACAGTGGACATTTCTAAATT15070.1988349617041489No Hit
CTGTAGGACGTGGAATATGGCAAGA13520.17838411959124706No Hit
GTCCTAAAGTGTGTATTTCTCATTT12100.1596485093974918No Hit
GGTATCAACGCAGAGTACTTTTTTT11190.14764188596346556No Hit
CTTTAGGACGTGAAATATGGCGAGG10710.14130872195430885No Hit
TATCAACGCAGAGTACTTTTTTTTT9880.13035762585514205No Hit
GTCCTACAGTGTGCATTTCTCATTT8530.11254560207938885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT402.7698348E-416.61628311
CGGTTTC508.699385E-515.201056513
GGTATCA4550.014.0335281
GTCTTAG1001.3802855E-712.3893011
CGAAATC550.003064353312.09174913
CCGGTTT550.003064353312.09174912
CAACACT802.8617162E-511.8750414
GTATTAG907.1874765E-611.6480611
CCAGTAC907.4460477E-611.6111513
TAGGACC2200.011.2273124
GTAGGAC7200.011.2153173
GTCCTAT855.1449835E-511.2120371
ATACTGG855.342753E-511.1706096
GGACATG2550.011.1706096
ATACTTA600.005893917311.0775216
GCGAGGA1803.6379788E-1211.0775219
GTATAAT1053.3287488E-610.8916931
TTACCCT700.001489830310.8571814
TGTAGGA7500.010.7667052
GTCCTAG803.6568733E-410.721511
Produced by FastQC (version 0.11.5)