FastQCFastQC Report
Thu 26 May 2016
SRR1512994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512994_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757915
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23070.3043876951900939No Hit
TATCAACGCAGAGTACTTTTTTTTT15670.20675141671559474No Hit
GGTATCAACGCAGAGTACTTTTTTT14660.1934253841129942No Hit
GTCCTACAGTGGACATTTCTAAATT14450.19065462485898813No Hit
CTGTAGGACGTGGAATATGGCAAGA14350.18933521569041384No Hit
CTTTAGGACGTGAAATATGGCGAGG12090.15951656848063436No Hit
GTCCTAAAGTGTGTATTTCTCATTT11460.1512042907186162No Hit
GTCCTACAGTGTGCATTTCTCATTT7720.10185838781393691No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7670.10119868322964977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT350.00214949816.3075396
GGTATCA3750.015.4668821
CACCGTG400.00525441414.2587247
TAGGACT1450.013.1236124
GGCGAGG3350.013.03037319
CTAGACA604.035025E-412.6861594
GTTAAAC604.0643982E-412.6752593
GATATAC1900.012.5109611
ACTGTTC2250.012.2503218
AAGGTAG550.003030832412.1095165
CCTCGAA550.003086844212.07994817
CTTAGAC951.0289586E-612.0081413
CTAGCAC1353.6561687E-1011.9710783
TAGCACA1302.5411282E-911.71034
GGCCTCG658.0763065E-411.68170815
TAAGGGT752.0417605E-411.4175434
AAGGCGT752.0455281E-411.41527756
ACTAGCA752.0581314E-411.4077332
TACACTG3100.011.3561595
GTATAAT600.00583276111.0930511
Produced by FastQC (version 0.11.5)