FastQCFastQC Report
Thu 26 May 2016
SRR1512993_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512993_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029040
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19060.18522117701935786No Hit
GGTATCAACGCAGAGTACTTTTTTT12280.11933452538288113No Hit
TATCAACGCAGAGTACTTTTTTTTT11370.11049133172665786No Hit
GTCCTAAAGTGTGTATTTCTCATTT11370.11049133172665786No Hit
GTCCTACAGTGGACATTTCTAAATT11320.1100054419653269No Hit
CTGTAGGACGTGGAATATGGCAAGA11200.10883930653813262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGG400.005297512314.24187110
TAGGACC1950.013.1501774
GGTATCA5250.012.33972551
GGCGAGG2600.012.05022819
ATAATTC1052.7260648E-711.7586243
GCAGTAC658.0381613E-411.6890473
TTAGTAC1251.8357241E-811.3968213
ATTATAC855.3337022E-511.1733533
GTTGGAC951.3630515E-510.9969323
GTATAGA1303.126297E-810.9926851
GTCCTAC6750.010.867831
TAGAACC1053.4734785E-610.8541154
CCTACAG7400.010.7807763
TCCTACA7250.010.7418312
GTTACAC1252.2447603E-710.63703253
TAGTACC909.5339026E-510.5526114
GAACTAG1002.322317E-510.4796921
GTATGGG1002.322317E-510.4796921
TGTAGGA7350.010.4664682
CTAGGAC1002.4035955E-510.4470853
Produced by FastQC (version 0.11.5)