##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512993_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1029040 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.239857537121978 31.0 30.0 34.0 25.0 34.0 2 29.389622366477493 31.0 30.0 34.0 23.0 34.0 3 29.52878994013838 31.0 30.0 34.0 25.0 34.0 4 33.00634377672394 35.0 35.0 37.0 26.0 37.0 5 33.13689749669595 37.0 35.0 37.0 27.0 37.0 6 33.15761000544197 37.0 35.0 37.0 27.0 37.0 7 32.9909517608645 37.0 35.0 37.0 26.0 37.0 8 32.96696046800902 37.0 35.0 37.0 26.0 37.0 9 34.321422879577085 39.0 35.0 39.0 26.0 39.0 10 34.20397263468864 39.0 35.0 39.0 25.0 39.0 11 34.26385854777268 39.0 35.0 39.0 26.0 39.0 12 34.16056713052942 39.0 35.0 39.0 25.0 39.0 13 34.1603387623416 39.0 35.0 39.0 25.0 39.0 14 35.18040115058695 39.0 35.0 41.0 25.0 41.0 15 35.12414872113815 39.0 35.0 41.0 25.0 41.0 16 35.00459068646506 39.0 35.0 41.0 24.0 41.0 17 34.95195716395864 39.0 35.0 41.0 24.0 41.0 18 34.6454287491254 39.0 34.0 41.0 19.0 41.0 19 34.88941440565964 39.0 34.0 41.0 22.0 41.0 20 34.97098460701236 39.0 35.0 41.0 24.0 41.0 21 34.915699098188604 39.0 35.0 41.0 23.0 41.0 22 34.82742458990904 39.0 35.0 41.0 22.0 41.0 23 34.568887506802454 39.0 34.0 41.0 18.0 41.0 24 34.56921791184016 39.0 34.0 41.0 18.0 41.0 25 34.49067577548006 39.0 34.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7487068263365799 1101 2 0.9403374969701552 1101 3 0.8853493558767234 1101 4 0.8963256716942851 1101 5 0.8210280017273988 1101 6 0.9622247543142706 1101 7 1.0287853977162058 1101 8 0.9126837486468347 1101 9 0.7731876297763876 1101 10 0.7930946613034777 1101 11 0.976350142436317 1101 12 1.1371587777835686 1101 13 0.8930654216194469 1101 14 0.9462444915779074 1101 15 0.6274045646351212 1101 16 1.3673558376893524 1101 17 1.321194501671414 1101 18 1.3515959464651104 1101 19 0.9459962052700064 1101 20 1.1355435950696773 1101 21 1.328422400544646 1101 22 1.3631612683663903 1101 23 1.5260380637884197 1101 24 1.2588874247618307 1101 25 1.4389408631148655 1102 1 0.6311907748568544 1102 2 0.6993985644623457 1102 3 0.7555765201467928 1102 4 0.7929908571702953 1102 5 0.7155465558991452 1102 6 0.6882957906850322 1102 7 0.9583420507947977 1102 8 0.8882145337299079 1102 9 0.8085029385799984 1102 10 1.0385038600724243 1102 11 1.0380040875643672 1102 12 1.1097930274564618 1102 13 0.9575103570562362 1102 14 1.0309820128203953 1102 15 1.2357825458735547 1102 16 1.130797642913059 1102 17 1.1049900842187697 1102 18 1.5146569679609954 1102 19 1.4444031723966049 1102 20 1.3616002575939419 1102 21 1.1081255436531237 1102 22 1.331249686413699 1102 23 1.5344447105835854 1102 24 1.4278084581346207 1102 25 1.2990344656032704 1103 1 0.32183243594624855 1103 2 0.6886659534261383 1103 3 0.5586939726429847 1103 4 0.8467727324915941 1103 5 0.4392379985858028 1103 6 0.7809245771676601 1103 7 0.6637174092759679 1103 8 0.5826285321973685 1103 9 0.8715915832544283 1103 10 0.7722166095542988 1103 11 0.7762295774751635 1103 12 0.8963039508263648 1103 13 0.8233478693019904 1103 14 1.0139907327637516 1103 15 1.063760081592342 1103 16 0.9654953871086533 1103 17 0.9223950523555757 1103 18 1.2361603418063396 1103 19 1.427555769788711 1103 20 1.2232669404266119 1103 21 1.1290613290461735 1103 22 1.0584604684927115 1103 23 1.2953058413633372 1103 24 1.1643874403875785 1103 25 1.0823930179051473 1104 1 0.36599088723577466 1104 2 0.41977857761579074 1104 3 0.42988197371705894 1104 4 0.5242056988501034 1104 5 0.45060526750035024 1104 6 0.42038619825017776 1104 7 0.6137122946442872 1104 8 0.5486609845353954 1104 9 0.45638098829489593 1104 10 0.6298556316367225 1104 11 0.5270655000907851 1104 12 0.47690575117671585 1104 13 0.5624920635557018 1104 14 0.6254497453841026 1104 15 0.8122736417595888 1104 16 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-2.3230312264541055 2216 21 -2.8146355496966677 2216 22 -3.404818222397491 2216 23 -3.3518054685641765 2216 24 -3.2227044849408273 2216 25 -3.3577756713977287 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43341.0 3 0.0 4 350.0 5 2179.0 6 2792.0 7 2484.0 8 2306.0 9 2139.0 10 2047.0 11 1896.0 12 1745.0 13 1850.0 14 1825.0 15 1783.0 16 1926.0 17 2003.0 18 2375.0 19 2830.0 20 3519.0 21 4483.0 22 5705.0 23 7141.0 24 8142.0 25 9168.0 26 10571.0 27 11801.0 28 13361.0 29 15837.0 30 19350.0 31 24311.0 32 30794.0 33 39408.0 34 50768.0 35 66827.0 36 91562.0 37 140248.0 38 400173.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.24514891421695 18.61147330427769 12.94201517667929 25.201362604826073 2 15.247878769024972 19.863062971978753 40.5560502718131 24.333007987183176 3 18.63438628553653 23.80132109128556 29.69986244708101 27.8644301760969 4 12.4285376824288 16.455410721802217 37.88229484324223 33.23375675252676 5 13.407903780402803 37.50207797232675 35.730916436834015 13.359101810436428 6 32.70797857037331 36.66800796224155 17.56630665515874 13.057706812226394 7 28.317709320464786 31.532125205930804 21.831046833160812 18.319118640443595 8 25.13078369125912 36.40724418204545 20.4279168505382 18.034055276157225 9 26.14197458865976 14.928287611582844 20.184636558738454 38.74510124101894 10 14.876233076633728 28.65286651134445 34.75407477821873 21.71682563380309 11 34.231712402063266 22.418168581906258 24.551320742613374 18.7987982734171 12 22.81265156676908 24.817633891471633 31.42402538011366 20.945689161645625 13 28.639841529650983 21.858974608198004 25.80975004762664 23.691433814524377 14 20.822774536020443 22.035007091040406 27.588083941588103 29.55413443135105 15 23.02007988405245 30.66933270862151 23.369730573584604 22.94085683374144 16 22.530180206450108 28.29056589102078 27.05526719931572 22.12398670321339 17 21.783018372754437 28.60296304648301 27.51412802802472 22.099890552737833 18 21.67810058213741 27.720694673598356 29.05307334308751 21.548131401176725 19 23.260687628003186 27.376424377152418 27.579188225310876 21.783699769533516 20 23.50635831071013 27.335730275045663 27.802093873413096 21.355817540831115 21 23.088684718031665 26.84706367227597 27.24307132414694 22.821180285545427 22 23.208483381689 27.755762585837378 27.092196513532297 21.943557518941326 23 22.520030365090644 27.556028160608392 27.414311444353835 22.509630029947132 24 23.01836617265135 26.825634107273377 27.947179758331647 22.20881996174362 25 22.880826275819903 27.197468140344803 27.642157096215136 22.27954848762015 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 152.0 1 152.0 2 238.5 3 325.0 4 325.0 5 325.0 6 595.0 7 865.0 8 865.0 9 865.0 10 1314.5 11 1764.0 12 1764.0 13 1764.0 14 3024.0 15 4284.0 16 4284.0 17 4284.0 18 7862.0 19 11440.0 20 11440.0 21 11440.0 22 18185.0 23 24930.0 24 24930.0 25 24930.0 26 35509.0 27 46088.0 28 46088.0 29 46088.0 30 59374.5 31 72661.0 32 72661.0 33 72661.0 34 83784.5 35 94908.0 36 94908.0 37 94908.0 38 103767.5 39 112627.0 40 112627.0 41 112627.0 42 120020.5 43 127414.0 44 127414.0 45 127414.0 46 130486.0 47 133558.0 48 133558.0 49 133558.0 50 129937.5 51 126317.0 52 126317.0 53 126317.0 54 114387.5 55 102458.0 56 102458.0 57 102458.0 58 88696.0 59 74934.0 60 74934.0 61 74934.0 62 61503.0 63 48072.0 64 48072.0 65 48072.0 66 37231.0 67 26390.0 68 26390.0 69 26390.0 70 19329.5 71 12269.0 72 12269.0 73 12269.0 74 8642.0 75 5015.0 76 5015.0 77 5015.0 78 3411.0 79 1807.0 80 1807.0 81 1807.0 82 1182.0 83 557.0 84 557.0 85 557.0 86 356.5 87 156.0 88 156.0 89 156.0 90 95.0 91 34.0 92 34.0 93 34.0 94 21.5 95 9.0 96 9.0 97 9.0 98 7.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35479670372385913 2 0.03809375728834642 3 0.033526393531835494 4 0.03410946124543263 5 0.03819093524061261 6 0.01788074321697893 7 0.017006141646583223 8 0.023517064448417942 9 0.02021301407136749 10 0.02050454792816606 11 0.018172277073777502 12 0.01972712431003654 13 0.019824302262302727 14 0.026918292777734585 15 0.029056207727590763 16 0.02137914949856177 17 0.022739640830288423 18 0.1072844593018736 19 0.02497473373241079 20 0.022253751068957474 21 0.02643240301640364 22 0.01992148021456892 23 0.022059395164425094 24 0.01865816683510845 25 0.022448106973489857 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1029040.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.33880353722218 #Duplication Level Percentage of deduplicated Percentage of total 1 88.32153473889011 71.83967962232526 2 8.671583694119473 14.106724849051261 3 1.7239808118318187 4.206796096665872 4 0.5423927749390888 1.7647031744311723 5 0.24319015204552283 0.9890397999708984 6 0.13141920558634748 0.6413688566523438 7 0.08224285060858184 0.4682674547594787 8 0.055564781002405125 0.3615658244434724 9 0.04238228513995285 0.310259192801144 >10 0.16462784962115948 2.2865945872847493 >50 0.011983271930428788 0.6808993049543762 >100 0.008020239566214582 1.3872797628968492 >500 3.5911490631121846E-4 0.21099339917010743 >1k 7.182298126224369E-4 0.7458280745929963 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1906 0.18522117701935786 No Hit GGTATCAACGCAGAGTACTTTTTTT 1228 0.11933452538288113 No Hit TATCAACGCAGAGTACTTTTTTTTT 1137 0.11049133172665786 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1137 0.11049133172665786 No Hit GTCCTACAGTGGACATTTCTAAATT 1132 0.1100054419653269 No Hit CTGTAGGACGTGGAATATGGCAAGA 1120 0.10883930653813262 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 9.717795226618985E-5 0.0 0.0 0.0 6 0.0 9.717795226618985E-5 0.0 0.0 0.0 7 0.0 9.717795226618985E-5 0.0 0.0 0.0 8 0.0 9.717795226618985E-5 0.0 0.0 0.0 9 0.0 9.717795226618985E-5 0.0 0.0 0.0 10 0.0 9.717795226618985E-5 0.0 9.717795226618985E-5 0.0 11 0.0 9.717795226618985E-5 0.0 9.717795226618985E-5 0.0 12 0.0 9.717795226618985E-5 0.0 9.717795226618985E-5 9.717795226618985E-5 13 0.0 9.717795226618985E-5 0.0 9.717795226618985E-5 9.717795226618985E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGTCGG 40 0.0052975123 14.241871 10 TAGGACC 195 0.0 13.150177 4 GGTATCA 525 0.0 12.3397255 1 GGCGAGG 260 0.0 12.050228 19 ATAATTC 105 2.7260648E-7 11.758624 3 GCAGTAC 65 8.0381613E-4 11.689047 3 TTAGTAC 125 1.8357241E-8 11.396821 3 ATTATAC 85 5.3337022E-5 11.173353 3 GTTGGAC 95 1.3630515E-5 10.996932 3 GTATAGA 130 3.126297E-8 10.992685 1 GTCCTAC 675 0.0 10.86783 1 TAGAACC 105 3.4734785E-6 10.854115 4 CCTACAG 740 0.0 10.780776 3 TCCTACA 725 0.0 10.741831 2 GTTACAC 125 2.2447603E-7 10.6370325 3 TAGTACC 90 9.5339026E-5 10.552611 4 GAACTAG 100 2.322317E-5 10.479692 1 GTATGGG 100 2.322317E-5 10.479692 1 TGTAGGA 735 0.0 10.466468 2 CTAGGAC 100 2.4035955E-5 10.447085 3 >>END_MODULE