FastQCFastQC Report
Thu 26 May 2016
SRR1512993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512993_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029040
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27200.2643240301640364No Hit
TATCAACGCAGAGTACTTTTTTTTT18390.17871025421752312No Hit
GGTATCAACGCAGAGTACTTTTTTT16400.15937184171655136No Hit
GTCCTACAGTGGACATTTCTAAATT11690.11360102619917592No Hit
CTGTAGGACGTGGAATATGGCAAGA11580.11253206872424784No Hit
GTCCTAAAGTGTGTATTTCTCATTT11050.1073816372541398No Hit
CTTTAGGACGTGAAATATGGCGAGG10360.10067635854777268No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGC551.1252161E-515.5488798
CGAGACG456.815808E-414.7616418
CGTGCGC400.005278299614.24967210
GTACTAG655.4006097E-513.1663521
GGTATCA5100.012.3058061
TCTAGAC701.0815602E-412.2247093
CGGACTG550.003029958812.1106615
GTAGATC550.003047697512.1012263
ATAAGAC802.8392526E-511.8851343
GAGACGT658.093214E-411.6795419
AGGACGT6350.011.5385035
TCTATAC1251.805347E-811.4097273
GGCGAGG2750.011.38755119
TAGGACG6650.011.304154
TTAGGAC4750.011.2095583
GACGTGA3700.011.04350957
CTAGGAC1553.074092E-1011.0416723
TGGATTG1303.18214E-810.97944455
GTATAGC1502.0936568E-910.7769031
GCTTAGG803.7387444E-410.696622
Produced by FastQC (version 0.11.5)