##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512993_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1029040 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.580963810930577 31.0 31.0 34.0 27.0 34.0 2 30.693700925134106 31.0 31.0 34.0 27.0 34.0 3 30.801031058073544 31.0 31.0 34.0 27.0 34.0 4 34.29369898157506 37.0 35.0 37.0 30.0 37.0 5 33.95502021301407 37.0 35.0 37.0 30.0 37.0 6 33.83013779833632 37.0 35.0 37.0 29.0 37.0 7 33.70736025810464 37.0 35.0 37.0 28.0 37.0 8 33.58910440799192 37.0 35.0 37.0 28.0 37.0 9 35.05252176786131 39.0 35.0 39.0 28.0 39.0 10 34.94463480525538 39.0 35.0 39.0 28.0 39.0 11 34.985812990748656 39.0 35.0 39.0 27.0 39.0 12 34.83595681411801 39.0 35.0 39.0 27.0 39.0 13 34.825690935240615 39.0 35.0 39.0 27.0 39.0 14 35.891268560988884 40.0 36.0 41.0 27.0 41.0 15 35.898971857265025 40.0 36.0 41.0 27.0 41.0 16 35.87306907408847 40.0 36.0 41.0 27.0 41.0 17 35.852658788774 40.0 36.0 41.0 27.0 41.0 18 35.772879577081554 40.0 36.0 41.0 27.0 41.0 19 35.76247084661432 40.0 36.0 41.0 27.0 41.0 20 35.73613853688875 40.0 36.0 41.0 27.0 41.0 21 35.68421441343388 40.0 36.0 41.0 27.0 41.0 22 35.64634027831765 40.0 36.0 41.0 27.0 41.0 23 35.540633988960586 40.0 36.0 41.0 27.0 41.0 24 35.52811163803156 40.0 36.0 41.0 27.0 41.0 25 35.4318141180129 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7339121071429524 1101 2 0.7178935466733485 1101 3 0.691779845770192 1101 4 0.489268871580677 1101 5 0.7317500765208678 1101 6 0.8350504275068644 1101 7 0.6371308406176794 1101 8 0.6664316327080186 1101 9 0.5305440182481078 1101 10 0.8270402161810111 1101 11 0.8488161965598238 1101 12 0.8771238030873363 1101 13 0.9900868095601538 1101 14 1.0298578528641755 1101 15 0.9307019938226659 1101 16 1.2921560472300442 1101 17 1.0525848721292448 1101 18 0.7496524018506463 1101 19 0.9723765598587732 1101 20 0.7621879026438663 1101 21 0.9760859650617064 1101 22 0.9153262545803713 1101 23 0.9120214700072538 1101 24 0.9246981369800267 1101 25 1.1208038482053553 1102 1 0.5287287338229483 1102 2 0.49776055163191657 1102 3 0.5143088378185823 1102 4 0.48793087181230277 1102 5 0.6381471774392296 1102 6 0.6395262790340652 1102 7 0.7102193859762025 1102 8 0.7369612227084161 1102 9 0.8250920593392408 1102 10 0.7682203829012053 1102 11 0.8797055926857809 1102 12 0.7486035454094448 1102 13 0.9048227948544749 1102 14 1.0255005239683896 1102 15 1.0257258503010434 1102 16 1.038460941976524 1102 17 1.0183307638785735 1102 18 1.1258734601107179 1102 19 1.1047527045303056 1102 20 1.18458473744073 1102 21 1.2293112508753765 1102 22 1.2718777306101359 1102 23 1.322366039855524 1102 24 1.1926635970375727 1102 25 1.2027955540852417 1103 1 0.5663745107773366 1103 2 0.4903411634182291 1103 3 0.4688629632215182 1103 4 0.45770609967215137 1103 5 0.6057658601684395 1103 6 0.6820517647417006 1103 7 0.7348604198245852 1103 8 0.6889869848547434 1103 9 0.8172386138784944 1103 10 0.8962947529928016 1103 11 0.9042892372147193 1103 12 1.0075716369478371 1103 13 0.8770875875577886 1103 14 0.899392748443077 1103 15 0.8264749829948528 1103 16 0.8283707679979742 1103 17 0.9595705671706227 1103 18 1.0665240125031232 1103 19 1.091168410127949 1103 20 1.0820049662867035 1103 21 0.7393131584640216 1103 22 1.0452456915271995 1103 23 0.9569997666694263 1103 24 0.8118539388192545 1103 25 1.0184424691264198 1104 1 0.39706262053733354 1104 2 0.45565596899828975 1104 3 0.460268783672376 1104 4 0.36017966341964325 1104 5 0.4340836578080385 1104 6 0.5932633570544112 1104 7 0.5471406504944696 1104 8 0.5439584068502228 1104 9 0.6593323522906545 1104 10 0.7600569481927195 1104 11 0.707714761784132 1104 12 0.6249348525167733 1104 13 0.7235399511569511 1104 14 0.924478418523762 1104 15 0.8002241358839797 1104 16 0.8018753775597816 1104 17 0.6322373361299185 1104 18 0.6099664153570927 1104 19 0.7926864967442384 1104 20 0.7852748142542794 1104 21 0.7264802279079348 1104 22 0.7633287915054652 1104 23 0.7012617892505304 1104 24 0.7257634993445308 1104 25 0.8543203317218371 1105 1 0.3334923609110483 1105 2 0.40833070064283916 1105 3 0.376469192178547 1105 4 0.2716371997026741 1105 5 0.3428146964693397 1105 6 0.4624746247040292 1105 7 0.3933301124093944 1105 8 0.44098315145578226 1105 9 0.6225117514617722 1105 10 0.6188568520001354 1105 11 0.5150094702550163 1105 12 0.4967625272484213 1105 13 0.4999101489451476 1105 14 0.8025203528691947 1105 15 0.6614569625405693 1105 16 0.6994806350467186 1105 17 0.5295134931736811 1105 18 0.6101514611578764 1105 19 0.6114052458658534 1105 20 0.6112283076379157 1105 21 0.49522021435352315 1105 22 0.5140451607975436 1105 23 0.7121896416886031 1105 24 0.5351581263015674 1105 25 0.6186149246366881 1106 1 0.4599686233006892 1106 2 0.3379728657908885 1106 3 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1107 15 0.43696804903716213 1107 16 0.11065650339464383 1107 17 0.1627080502571232 1107 18 0.3108369203320578 1107 19 0.36246787489938015 1107 20 0.5421699219768001 1107 21 0.3805160604280644 1107 22 0.629705821847736 1107 23 0.48997243260645007 1107 24 0.4225455797404578 1107 25 0.5262804043171201 1108 1 0.4729849056790556 1108 2 0.42788911357313 1108 3 0.4408085671820672 1108 4 0.5687709582624336 1108 5 0.5382009208604615 1108 6 0.5498419448574765 1108 7 0.5139679896069538 1108 8 0.6048330216158888 1108 9 0.5018276839337688 1108 10 0.7114331646222993 1108 11 0.8207072165048714 1108 12 0.7724932639090838 1108 13 0.6264479959124571 1108 14 0.7691728119409973 1108 15 0.6559407855978066 1108 16 0.6690355743139449 1108 17 0.8026369171164234 1108 18 0.6745392796786973 1108 19 0.6771742679264321 1108 20 0.7642486107290978 1108 21 0.8264221581027158 1108 22 0.9853251023648966 1108 23 0.7914954348197654 1108 24 0.7356410593499945 1108 25 0.8351360416916265 1109 1 -0.10636278824030043 1109 2 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12 -1.3296169708723866 2216 13 -1.8526451647185809 2216 14 -1.951456470160366 2216 15 -1.9727332787886311 2216 16 -1.6951865704917566 2216 17 -2.060804907222952 2216 18 -1.6851330580654746 2216 19 -1.9114386500989866 2216 20 -2.431886528059053 2216 21 -2.0562586872095565 2216 22 -2.5373685357103284 2216 23 -1.5961515534554422 2216 24 -1.8230181372568737 2216 25 -1.5784143878748864 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23374.0 3 0.0 4 369.0 5 3298.0 6 3987.0 7 3011.0 8 2521.0 9 2176.0 10 1944.0 11 1874.0 12 1649.0 13 1577.0 14 1485.0 15 1640.0 16 1588.0 17 1723.0 18 1967.0 19 2271.0 20 2911.0 21 3744.0 22 4902.0 23 5896.0 24 7063.0 25 8161.0 26 9391.0 27 10414.0 28 11790.0 29 14159.0 30 17874.0 31 23019.0 32 29113.0 33 38168.0 34 49397.0 35 64282.0 36 88485.0 37 136154.0 38 447663.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.926439267660314 19.336339040676247 13.78281495255119 26.95440673911225 2 14.211997796001901 20.22265434060322 39.55340856857709 26.011939294817786 3 17.03383736297909 23.850093290834177 30.2647127419731 28.851356604213635 4 11.700017011348999 16.970521762375757 36.998225959318574 34.331235266956675 5 13.06593897122251 37.79614089309315 35.75115072028817 13.386769415396166 6 32.69655130158922 37.10280546524508 17.225986464289083 12.974656768876619 7 28.168330836511473 31.722923741130288 21.665576231163254 18.443169191194986 8 25.257649751928845 36.58743170993082 20.08802587486167 18.066892663278665 9 25.887900442370327 14.747848913519032 20.00486121238637 39.359389431724274 10 14.545093701387207 29.049910837022917 34.638722123800825 21.76627333778905 11 34.22715478284532 22.437924566808228 24.384104801795935 18.95081584855052 12 22.65376477437401 24.846606984399738 31.485295361130845 21.014332880095406 13 28.559419301104892 21.81887271409093 25.890043120916413 23.731664863887765 14 20.879359038402107 22.001469453444955 27.46021688044363 29.658954627709306 15 22.95771349045684 30.653467540431716 23.46657829238734 22.9222406767241 16 22.435330457619507 28.305888344805734 27.071301244306728 22.18747995326803 17 21.74370758763885 28.606770035937203 27.549845004418028 22.09967737200592 18 21.617702936851995 27.688777845479272 29.062308374804545 21.63121084286418 19 23.170524306679223 27.251099857433985 27.726708629859953 21.85166720602684 20 23.420073626881976 27.214345968597787 27.931080729116047 21.43449967540419 21 22.966326769591376 26.7166292368496 27.417089958864455 22.899954034694574 22 23.086461503776352 27.65306558759849 27.307013554433468 21.95345935419169 23 22.40332970859219 27.347554368687675 27.686122683396842 22.56299323932329 24 22.982682888906165 26.74094301484879 28.106681955997825 22.16969214024722 25 22.71405150472355 27.111749059517408 27.87628292903839 22.297916506720647 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 90.0 1 90.0 2 186.0 3 282.0 4 282.0 5 282.0 6 625.0 7 968.0 8 968.0 9 968.0 10 1383.5 11 1799.0 12 1799.0 13 1799.0 14 3089.5 15 4380.0 16 4380.0 17 4380.0 18 8123.5 19 11867.0 20 11867.0 21 11867.0 22 18586.5 23 25306.0 24 25306.0 25 25306.0 26 36028.0 27 46750.0 28 46750.0 29 46750.0 30 59822.5 31 72895.0 32 72895.0 33 72895.0 34 83883.5 35 94872.0 36 94872.0 37 94872.0 38 103662.0 39 112452.0 40 112452.0 41 112452.0 42 119413.0 43 126374.0 44 126374.0 45 126374.0 46 129796.5 47 133219.0 48 133219.0 49 133219.0 50 130165.5 51 127112.0 52 127112.0 53 127112.0 54 114930.0 55 102748.0 56 102748.0 57 102748.0 58 88962.5 59 75177.0 60 75177.0 61 75177.0 62 61448.5 63 47720.0 64 47720.0 65 47720.0 66 36873.5 67 26027.0 68 26027.0 69 26027.0 70 18947.0 71 11867.0 72 11867.0 73 11867.0 74 8285.5 75 4704.0 76 4704.0 77 4704.0 78 3213.0 79 1722.0 80 1722.0 81 1722.0 82 1124.5 83 527.0 84 527.0 85 527.0 86 333.5 87 140.0 88 140.0 89 140.0 90 91.0 91 42.0 92 42.0 93 42.0 94 21.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07521573505403094 2 9.717795226618985E-5 3 0.0 4 0.030611054963849804 5 0.07492420119723238 6 0.07745082795615331 7 0.03877400295420975 8 0.06860763429993003 9 0.047617196610433024 10 0.11146311124931975 11 0.004858897613309493 12 0.014285158983129908 13 0.007093990515431858 14 0.008357303894892327 15 0.008065770038093757 16 0.01885252273964083 17 0.029250563632123142 18 0.001263313379460468 19 0.004178651947446163 20 0.00660810075410091 21 2.9153385679856955E-4 22 3.887118090647594E-4 23 9.717795226618985E-5 24 0.0 25 0.004567363756510923 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1029040.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.39942029280296 #Duplication Level Percentage of deduplicated Percentage of total 1 87.8735664985728 70.64983805546325 2 8.951979985663097 14.394680026401751 3 1.7958639789483872 4.3315926849653055 4 0.6012809588994007 1.9337056211445003 5 0.25769686372840256 1.0359339227518505 6 0.14234910496158032 0.6866871310866252 7 0.07698625022826676 0.43327549222085215 8 0.06548160679082596 0.4211746580658941 9 0.038282732737644626 0.2770118567397844 >10 0.1737503560873558 2.3720753069633926 >50 0.013323625553926088 0.748352701090839 >100 0.007865032882307365 1.3100217425172271 >500 7.260022827638247E-4 0.3679265920013026 >1k 8.470026632244622E-4 1.037724208587492 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2720 0.2643240301640364 No Hit TATCAACGCAGAGTACTTTTTTTTT 1839 0.17871025421752312 No Hit GGTATCAACGCAGAGTACTTTTTTT 1640 0.15937184171655136 No Hit GTCCTACAGTGGACATTTCTAAATT 1169 0.11360102619917592 No Hit CTGTAGGACGTGGAATATGGCAAGA 1158 0.11253206872424784 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1105 0.1073816372541398 No Hit CTTTAGGACGTGAAATATGGCGAGG 1036 0.10067635854777268 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 9.717795226618985E-5 0.0 0.0 0.0 6 0.0 9.717795226618985E-5 0.0 0.0 0.0 7 0.0 9.717795226618985E-5 0.0 0.0 0.0 8 0.0 9.717795226618985E-5 0.0 0.0 0.0 9 0.0 9.717795226618985E-5 0.0 0.0 0.0 10 0.0 9.717795226618985E-5 0.0 0.0 0.0 11 0.0 9.717795226618985E-5 0.0 0.0 0.0 12 0.0 9.717795226618985E-5 0.0 0.0 9.717795226618985E-5 13 0.0 9.717795226618985E-5 0.0 0.0 9.717795226618985E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGTGC 55 1.1252161E-5 15.548879 8 CGAGACG 45 6.815808E-4 14.76164 18 CGTGCGC 40 0.0052782996 14.249672 10 GTACTAG 65 5.4006097E-5 13.166352 1 GGTATCA 510 0.0 12.305806 1 TCTAGAC 70 1.0815602E-4 12.224709 3 CGGACTG 55 0.0030299588 12.110661 5 GTAGATC 55 0.0030476975 12.101226 3 ATAAGAC 80 2.8392526E-5 11.885134 3 GAGACGT 65 8.093214E-4 11.67954 19 AGGACGT 635 0.0 11.538503 5 TCTATAC 125 1.805347E-8 11.409727 3 GGCGAGG 275 0.0 11.387551 19 TAGGACG 665 0.0 11.30415 4 TTAGGAC 475 0.0 11.209558 3 GACGTGA 370 0.0 11.0435095 7 CTAGGAC 155 3.074092E-10 11.041672 3 TGGATTG 130 3.18214E-8 10.9794445 5 GTATAGC 150 2.0936568E-9 10.776903 1 GCTTAGG 80 3.7387444E-4 10.69662 2 >>END_MODULE