FastQCFastQC Report
Thu 26 May 2016
SRR1512992_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512992_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913418
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27020.1412132633852091No Hit
GTCCTACAGTGGACATTTCTAAATT21040.10996029095576607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACTC707.306575E-613.5638689
CGTGCGC604.1120782E-412.6596110
GGTATCA8700.012.5992141
TAGGACC4400.011.2248334
GGCGAGG4300.011.03978119
GGTCTAC2900.010.846281
AGGACGT10400.010.6843385
TAGGACT2350.010.5083534
TAGACTG2100.010.4016845
GTCCTAA6350.010.3571481
GTATCAA17700.010.339341
GTCCTAG2500.010.2941051
TTAGGAC7100.010.1668033
GTAGGAC10100.010.1562073
GCCGGTT750.002660319210.12768711
GTCCTAC11500.010.1117621
GGACGTG10500.010.0364756
GTGCGCC1054.1307685E-59.946835511
GACGTGA5950.09.8928697
TCCTACA13350.09.7469432
Produced by FastQC (version 0.11.5)