FastQCFastQC Report
Thu 26 May 2016
SRR1512992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512992_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913418
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33190.17345922323297888No Hit
TATCAACGCAGAGTACTTTTTTTTT23380.12218971494989594No Hit
GTCCTACAGTGGACATTTCTAAATT20870.1090718285288421No Hit
GGTATCAACGCAGAGTACTTTTTTT20350.1063541787523688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.011.614641
TAGGACC4200.011.5536734
ATAGGAC2150.010.6130963
CCCCTAG909.435271E-510.5645141
TAGGACT3000.010.4662694
GTATAAG2300.010.3348511
AATCCCG1409.825999E-810.16884219
TGGCGAG9800.09.97514918
TACACCG1151.0077296E-59.9284755
GTCCTAC10950.09.8988061
CTAATAC1252.5441332E-69.8878693
GGACGTG9350.09.8686316
CTAGGAC2350.09.7098543
GGCGAGG3950.09.61105619
GTATCAA15850.09.5980451
AGGACGT9900.09.514795
GTACCGT800.0044673459.5125476
GGACTTA1002.719295E-49.5125466
GTATTAG1401.0463809E-69.5080631
GGCTAGG2002.6011548E-109.5080631
Produced by FastQC (version 0.11.5)