##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512992_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1913418 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.561251644962052 31.0 31.0 34.0 27.0 34.0 2 30.703918850977676 31.0 31.0 34.0 27.0 34.0 3 30.79455403889793 31.0 31.0 34.0 27.0 34.0 4 34.28440988848229 37.0 35.0 37.0 30.0 37.0 5 33.93320591736882 37.0 35.0 37.0 30.0 37.0 6 33.797616098521075 37.0 35.0 37.0 29.0 37.0 7 33.681462179199734 37.0 35.0 37.0 28.0 37.0 8 33.57102211853343 37.0 35.0 37.0 28.0 37.0 9 35.04443252859542 39.0 35.0 39.0 28.0 39.0 10 34.93667666970834 39.0 35.0 39.0 28.0 39.0 11 34.961787231018 39.0 35.0 39.0 27.0 39.0 12 34.81311036062167 39.0 35.0 39.0 27.0 39.0 13 34.79948500536736 39.0 35.0 39.0 27.0 39.0 14 35.87396742374118 40.0 36.0 41.0 27.0 41.0 15 35.88238011767424 40.0 36.0 41.0 27.0 41.0 16 35.85600062296895 40.0 36.0 41.0 27.0 41.0 17 35.83164107372252 40.0 36.0 41.0 27.0 41.0 18 35.74875327816504 40.0 36.0 41.0 27.0 41.0 19 35.74426079403455 40.0 36.0 41.0 27.0 41.0 20 35.7126979050056 40.0 36.0 41.0 27.0 41.0 21 35.65938388789068 40.0 36.0 41.0 27.0 41.0 22 35.61265337735926 40.0 36.0 41.0 27.0 41.0 23 35.50702512467218 40.0 36.0 41.0 27.0 41.0 24 35.48970899197143 40.0 36.0 41.0 27.0 41.0 25 35.39156211554402 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5770091267537794 1101 2 0.5953872191658292 1101 3 0.6771652779570161 1101 4 0.4397673896801422 1101 5 0.7169379008963261 1101 6 0.7011758296862354 1101 7 0.691251704626545 1101 8 0.7304810603479837 1101 9 0.600620502757856 1101 10 0.9035383476683094 1101 11 0.9863676053102353 1101 12 0.8986857769622105 1101 13 1.0860005188122912 1101 14 1.1872909561558274 1101 15 1.0944720669252703 1101 16 1.2540355168544295 1101 17 1.0565745144214276 1101 18 0.6084845722936691 1101 19 1.0327025666910927 1101 20 0.9431718462467273 1101 21 1.1227546743302241 1101 22 1.1259179538466881 1101 23 1.0535750626142786 1101 24 1.066596516591126 1101 25 1.1827539844140276 1102 1 0.7360678969267127 1102 2 0.7412267145925 1102 3 0.7219457384167995 1102 4 0.664616189515634 1102 5 0.7965225687630166 1102 6 0.6781788973523604 1102 7 0.7493824283309536 1102 8 0.7349466413594428 1102 9 0.9827216991194732 1102 10 1.0876180337466224 1102 11 1.008166626699385 1102 12 1.0393300995263175 1102 13 1.092777413004569 1102 14 1.3323388866784 1102 15 1.271679786514106 1102 16 1.2023943577329845 1102 17 1.211361369373492 1102 18 1.2058576190861388 1102 19 1.288717474027294 1102 20 1.2781418315703803 1102 21 1.2164396202305667 1102 22 1.381034251914535 1102 23 1.3164605279614818 1102 24 1.252091723538868 1102 25 1.2588315408728903 1103 1 0.4228590956416163 1103 2 0.40486875002428846 1103 3 0.4058283558718614 1103 4 0.5036398666891202 1103 5 0.554461223348568 1103 6 0.5928885810984212 1103 7 0.5256598429895405 1103 8 0.5173465227662177 1103 9 0.7657158068051473 1103 10 0.7237294178940346 1103 11 0.7246559648800144 1103 12 0.7905121939660233 1103 13 0.7306705595031744 1103 14 0.942763359223818 1103 15 0.6819511601585972 1103 16 0.7593506086108022 1103 17 0.850434261817739 1103 18 0.9023879654099645 1103 19 0.8924331582329756 1103 20 0.9157492637178066 1103 21 0.6121529655652793 1103 22 0.8493317586844995 1103 23 0.8612568493720261 1103 24 0.8406386564170774 1103 25 0.846513426010219 1104 1 0.601458952092198 1104 2 0.6590594742574858 1104 3 0.547349502120337 1104 4 0.5791476113530933 1104 5 0.6324982532765304 1104 6 0.8048687943848378 1104 7 0.718413258201231 1104 8 0.6958139052999499 1104 9 0.934209211082333 1104 10 0.9092954692752713 1104 11 0.9782608473796301 1104 12 0.799964236361312 1104 13 0.9211764665890385 1104 14 0.9916089371648766 1104 15 1.0445700228069157 1104 16 1.0656167727028887 1104 17 0.983749302758433 1104 18 1.0660531786918384 1104 19 1.1316263568287752 1104 20 1.1552640384825779 1104 21 0.8795910814837029 1104 22 1.0938819068278605 1104 23 1.0254167077511553 1104 24 1.0820429754533833 1104 25 1.1928459566918619 1105 1 0.17605557224795376 1105 2 0.2400765902492914 1105 3 0.26485723567731156 1105 4 0.15054797595410463 1105 5 0.13887699412374843 1105 6 0.29191075548339995 1105 7 0.3460646999921124 1105 8 0.45690318635011096 1105 9 0.39243156794712064 1105 10 0.27876981838606696 1105 11 0.32225976708033954 1105 12 0.4477837819604247 1105 13 0.3942782581445812 1105 14 0.5485069945806487 1105 15 0.5863643235174578 1105 16 0.5086489755717238 1105 17 0.26699404740446653 1105 18 0.5233400461259663 1105 19 0.4957096482590515 1105 20 0.4832159385122523 1105 21 0.45867236512090415 1105 22 0.5003247460249582 1105 23 0.496990444396225 1105 24 0.5590071034759561 1105 25 0.5952254580129193 1106 1 0.3535780149270771 1106 2 0.32405121782318247 1106 3 0.39561536285028964 1106 4 0.3054382830448219 1106 5 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0.7857190040045339 1107 18 0.9533386742394612 1107 19 1.0713640122386607 1107 20 1.078696003879628 1107 21 0.9484971087765715 1107 22 1.0677682829727075 1107 23 0.9005278408144761 1107 24 1.0598392950085724 1107 25 1.0005769370618793 1108 1 0.554075603099438 1108 2 0.49372407922159667 1108 3 0.42643643704902345 1108 4 0.508441215525572 1108 5 0.48854568417618083 1108 6 0.5062771964097905 1108 7 0.5394481057881677 1108 8 0.5963884537228097 1108 9 0.4234528562452411 1108 10 0.6693343815627983 1108 11 0.7515445988798959 1108 12 0.778833583006481 1108 13 0.6236669762704352 1108 14 0.8036206073288668 1108 15 0.7440728762866087 1108 16 0.6702164402005195 1108 17 0.6671311627111791 1108 18 0.636613290206931 1108 19 0.6473296310437249 1108 20 0.7585604657551741 1108 21 0.8877570262316041 1108 22 0.8984552013222853 1108 23 0.8943305538860358 1108 24 0.8842078071636834 1108 25 0.767040391327761 1109 1 0.24669273382016854 1109 2 0.20114776023689274 1109 3 0.2368690928208288 1109 4 0.3098480727934714 1109 5 0.2525516566360153 1109 6 0.08315884532633788 1109 7 0.12327747731912808 1109 8 0.34919469776540524 1109 9 0.5605166780839852 1109 10 0.4123049316435754 1109 11 0.5974683819245286 1109 12 0.5445031747328315 1109 13 0.60139386749929 1109 14 0.6818276321281687 1109 15 0.6537525915968772 1109 16 0.589858962619374 1109 17 0.5683101453447676 1109 18 0.5859421426287881 1109 19 0.8064552893297048 1109 20 0.6848276269863405 1109 21 0.7538501572142167 1109 22 0.8157717608208017 1109 23 0.5878468762305502 1109 24 0.1926419010737419 1109 25 0.3852747995559511 1110 1 0.2269192393978301 1110 2 0.24508154337613774 1110 3 0.2866611220232542 1110 4 0.3536739086385552 1110 5 0.3279777230748948 1110 6 0.2818783301191701 1110 7 0.28916720418185093 1110 8 0.5523484430388805 1110 9 0.49892853605596343 1110 10 0.5307881879464347 1110 11 0.4084174530036151 1110 12 0.538282807604844 1110 13 0.12703977128226995 1110 14 0.5618200987850841 1110 15 0.6231912580114098 1110 16 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18 -1.9952574359445379 2216 19 -2.2289821033170227 2216 20 -2.4329726383733288 2216 21 -2.1776210969374645 2216 22 -2.5285867603230727 2216 23 -1.7236153754638792 2216 24 -1.9491036648613616 2216 25 -1.7795250891376142 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45422.0 3 0.0 4 728.0 5 6568.0 6 7686.0 7 6051.0 8 5042.0 9 4408.0 10 3680.0 11 3471.0 12 3199.0 13 3025.0 14 2997.0 15 2985.0 16 3056.0 17 3225.0 18 3610.0 19 4268.0 20 5297.0 21 6851.0 22 8938.0 23 10902.0 24 12753.0 25 14861.0 26 16992.0 27 18643.0 28 21469.0 29 25945.0 30 32376.0 31 41821.0 32 52824.0 33 67984.0 34 89553.0 35 117765.0 36 161397.0 37 250546.0 38 847080.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.94194120370007 18.997099675446098 13.596742430780576 27.46421669007325 2 14.089005306744905 20.068317677198973 39.49464151511382 26.348035500942295 3 17.16613933808504 23.740029622382565 29.99632072030262 29.097510319229773 4 11.673972309131923 16.717348744448888 37.02123210509669 34.58744684132249 5 13.189928018425276 37.63526616029236 35.67768529345932 13.497120527823045 6 32.851531239375475 36.853205010853365 17.283416586029237 13.011847163741924 7 28.340322262750632 31.380702608288324 21.700795749866554 18.578179379094482 8 25.518630212775527 36.46227547289987 20.021523853250827 17.997570461073778 9 26.0975043553005 14.909976513793701 19.686376536380614 39.306142594525184 10 14.642617794637658 28.8111953233189 34.38316955637134 22.163017325672097 11 34.68317726383524 22.281832402421504 24.09424900841819 18.94074132532507 12 22.84207021598768 24.518152989921134 31.388435462685504 21.251341331405683 13 28.62473155314764 21.448831638810216 25.870306132635353 24.05613067540679 14 21.118799979093712 21.44833533685256 27.291172320075262 30.141692363978468 15 23.088333061552163 30.055809217981043 23.28704690942997 23.568810811036826 16 22.769402860249226 28.050880237517774 26.55949443840428 22.62022246382872 17 21.995165541518944 28.249014341320716 27.309776500391546 22.446043616768797 18 22.00641684623349 27.58621951174898 28.520733500191543 21.886630141825993 19 23.53952024735531 26.903809579060095 27.299814305821013 22.256855867763583 20 23.586131418906913 27.039531038000725 27.519217783427024 21.855119759665335 21 23.256625846130692 26.515143596583286 27.081042491543922 23.147188065742107 22 23.297198817187777 27.22684051372265 27.01695301467645 22.459007654413124 23 22.799670537227097 27.046102837958042 27.167560877968118 22.986665746846743 24 23.182453171472815 26.608731917820315 27.6301506676276 22.578664243079267 25 23.026841870349134 26.828647495445175 27.38918151058299 22.755329123622705 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 104.0 1 104.0 2 218.5 3 333.0 4 333.0 5 333.0 6 797.0 7 1261.0 8 1261.0 9 1261.0 10 1909.5 11 2558.0 12 2558.0 13 2558.0 14 4770.0 15 6982.0 16 6982.0 17 6982.0 18 13180.5 19 19379.0 20 19379.0 21 19379.0 22 30986.0 23 42593.0 24 42593.0 25 42593.0 26 61673.0 27 80753.0 28 80753.0 29 80753.0 30 104864.0 31 128975.0 32 128975.0 33 128975.0 34 149391.0 35 169807.0 36 169807.0 37 169807.0 38 188177.5 39 206548.0 40 206548.0 41 206548.0 42 221272.0 43 235996.0 44 235996.0 45 235996.0 46 243234.0 47 250472.0 48 250472.0 49 250472.0 50 245506.5 51 240541.0 52 240541.0 53 240541.0 54 219374.0 55 198207.0 56 198207.0 57 198207.0 58 172022.0 59 145837.0 60 145837.0 61 145837.0 62 119685.5 63 93534.0 64 93534.0 65 93534.0 66 72662.5 67 51791.0 68 51791.0 69 51791.0 70 37705.0 71 23619.0 72 23619.0 73 23619.0 74 16503.5 75 9388.0 76 9388.0 77 9388.0 78 6394.0 79 3400.0 80 3400.0 81 3400.0 82 2195.5 83 991.0 84 991.0 85 991.0 86 639.0 87 287.0 88 287.0 89 287.0 90 170.5 91 54.0 92 54.0 93 54.0 94 30.5 95 7.0 96 7.0 97 7.0 98 4.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0816862807813034 2 2.090499828056389E-4 3 0.0 4 0.026810660294823194 5 0.06595526957517908 6 0.08194759325981045 7 0.03297763478758954 8 0.058586257681280306 9 0.042123571535336246 10 0.10332295400168703 11 0.0031880122377859936 12 0.010818336610191813 13 0.0050694620830367436 14 0.006166974492766348 15 0.005957924509960709 16 0.015574223719020099 17 0.025295047919482307 18 8.884624269239653E-4 19 0.0029789622549803545 20 0.0044945746303212365 21 1.0452499140281945E-4 22 6.271499484169167E-4 23 0.0 24 5.2262495701409724E-5 25 0.0033447997248902224 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1913418.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.58996588348252 #Duplication Level Percentage of deduplicated Percentage of total 1 79.5814357646349 55.38069399819497 2 13.358304580756334 18.59207920072003 3 3.772987942580435 7.876863066088902 4 1.4120468073751657 3.930571566044728 5 0.6796464484579686 2.364828658051005 6 0.3402289110429836 1.4205910987233379 7 0.21082628177081178 1.0269975629040584 8 0.14289040643304715 0.795499080700216 9 0.09510354077965674 0.595642694242923 >10 0.3789349260983164 4.336152031093499 >50 0.01456328349199091 0.7071304809009754 >100 0.011224156515155848 1.5802657229709378 >500 0.001204633375561546 0.5647025017369398 >1k 6.023166877807662E-4 0.8279823376274618 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3319 0.17345922323297888 No Hit TATCAACGCAGAGTACTTTTTTTTT 2338 0.12218971494989594 No Hit GTCCTACAGTGGACATTTCTAAATT 2087 0.1090718285288421 No Hit GGTATCAACGCAGAGTACTTTTTTT 2035 0.1063541787523688 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.226249570140973E-5 0.0 3 0.0 0.0 0.0 5.226249570140973E-5 0.0 4 0.0 0.0 0.0 5.226249570140973E-5 0.0 5 0.0 0.0 0.0 5.226249570140973E-5 0.0 6 0.0 0.0 0.0 5.226249570140973E-5 0.0 7 0.0 0.0 0.0 5.226249570140973E-5 5.226249570140973E-5 8 0.0 0.0 0.0 5.226249570140973E-5 5.226249570140973E-5 9 0.0 0.0 0.0 5.226249570140973E-5 5.226249570140973E-5 10 0.0 0.0 0.0 1.0452499140281946E-4 5.226249570140973E-5 11 0.0 0.0 0.0 1.0452499140281946E-4 5.226249570140973E-5 12 0.0 0.0 0.0 1.0452499140281946E-4 5.226249570140973E-5 13 0.0 5.226249570140973E-5 0.0 1.0452499140281946E-4 5.226249570140973E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 835 0.0 11.61464 1 TAGGACC 420 0.0 11.553673 4 ATAGGAC 215 0.0 10.613096 3 CCCCTAG 90 9.435271E-5 10.564514 1 TAGGACT 300 0.0 10.466269 4 GTATAAG 230 0.0 10.334851 1 AATCCCG 140 9.825999E-8 10.168842 19 TGGCGAG 980 0.0 9.975149 18 TACACCG 115 1.0077296E-5 9.928475 5 GTCCTAC 1095 0.0 9.898806 1 CTAATAC 125 2.5441332E-6 9.887869 3 GGACGTG 935 0.0 9.868631 6 CTAGGAC 235 0.0 9.709854 3 GGCGAGG 395 0.0 9.611056 19 GTATCAA 1585 0.0 9.598045 1 AGGACGT 990 0.0 9.51479 5 GTACCGT 80 0.004467345 9.512547 6 GGACTTA 100 2.719295E-4 9.512546 6 GTATTAG 140 1.0463809E-6 9.508063 1 GGCTAGG 200 2.6011548E-10 9.508063 1 >>END_MODULE