FastQCFastQC Report
Thu 26 May 2016
SRR1512991_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512991_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3056299
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41740.13657040754193225No Hit
GTCCTACAGTGGACATTTCTAAATT37430.12246838414696991No Hit
CTGTAGGACGTGGAATATGGCAAGA33440.10941337873028785No Hit
GTCCTAAAGTGTGTATTTCTCATTT32990.10794100969833123No Hit
CTTTAGGACGTGAAATATGGCGAGG31860.10424372746252902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12450.012.3211851
TAGGACC7150.012.0872844
GGACGTG17800.010.5613096
AGGACGT18650.010.5409035
GTCCTAC21550.010.478451
GTAGGAC19700.010.2202853
GTATCAA25800.010.1926091
TCCAACG2450.010.08422318
GGCGAGG9350.09.95162919
TAGGACG19900.09.8312234
TCCTACA24400.09.7306892
GTCCTAA12000.09.6866711
TTAGGAC12400.09.6503323
GACGTGG9500.09.5944527
TGTAGGA23000.09.5797362
GATATAC6800.09.5278731
AAGCCGT800.0045391489.494516
AATCCCG3750.09.36807119
GCTCTAA2650.09.3481031
TGGCGAG20350.09.33900718
Produced by FastQC (version 0.11.5)