##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512991_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3056299
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.08008084287565	31.0	30.0	34.0	23.0	34.0
2	29.255770459631076	31.0	30.0	34.0	23.0	34.0
3	29.38388750577087	31.0	30.0	34.0	23.0	34.0
4	32.847790088600625	35.0	33.0	37.0	26.0	37.0
5	32.97272223692774	37.0	35.0	37.0	26.0	37.0
6	32.986056010881136	37.0	35.0	37.0	26.0	37.0
7	32.83434245144209	37.0	35.0	37.0	25.0	37.0
8	32.81124850677241	37.0	35.0	37.0	25.0	37.0
9	34.15338944259053	39.0	35.0	39.0	25.0	39.0
10	34.03751432696866	39.0	34.0	39.0	25.0	39.0
11	34.091687364358	39.0	35.0	39.0	25.0	39.0
12	33.989576281639984	39.0	35.0	39.0	25.0	39.0
13	33.992834143518024	39.0	35.0	39.0	25.0	39.0
14	35.00024997554232	39.0	35.0	41.0	24.0	41.0
15	34.935086521312215	39.0	35.0	41.0	23.0	41.0
16	34.823251259120916	39.0	34.0	41.0	22.0	41.0
17	34.76254941025076	39.0	34.0	41.0	21.0	41.0
18	34.460090455809464	39.0	34.0	41.0	17.0	41.0
19	34.706307857968085	39.0	34.0	41.0	19.0	41.0
20	34.77892444423795	39.0	34.0	41.0	19.0	41.0
21	34.722764035848584	39.0	34.0	41.0	19.0	41.0
22	34.63269725900509	39.0	34.0	41.0	18.0	41.0
23	34.36847180200628	39.0	34.0	41.0	17.0	41.0
24	34.36249169338471	39.0	34.0	41.0	16.0	41.0
25	34.28742999294244	39.0	34.0	41.0	16.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.4724585652273703
1101	2	0.7613126299920836
1101	3	0.7357571117729584
1101	4	0.635639313347184
1101	5	0.517206143553949
1101	6	0.6846175418218863
1101	7	0.690706464187059
1101	8	0.4979763619341995
1101	9	0.44837753783920675
1101	10	0.49091209983390627
1101	11	0.692695219457427
1101	12	0.8870647297361316
1101	13	0.6677369004266254
1101	14	0.5698942863307792
1101	15	0.29464875697667736
1101	16	0.8516295946664485
1101	17	0.9499898652741479
1101	18	1.1316934535396825
1101	19	0.5903530185615153
1101	20	0.6589422230984212
1101	21	0.7521236067415913
1101	22	0.7072401091732203
1101	23	1.0043874028771
1101	24	0.6321208778719196
1101	25	0.8498418392367597
1102	1	0.7505023005868914
1102	2	0.8924059226555201
1102	3	0.8737358240045197
1102	4	0.99395693123585
1102	5	0.951224563729383
1102	6	0.8729293605973112
1102	7	1.026186078249225
1102	8	1.049753310687393
1102	9	1.017069632323654
1102	10	1.1292542840447979
1102	11	1.1431328926888114
1102	12	1.1674628137456509
1102	13	1.128268227053681
1102	14	1.1101766620493905
1102	15	1.336925662350673
1102	16	1.2907452983911227
1102	17	1.1287281243529534
1102	18	1.477122428101211
1102	19	1.41187823100541
1102	20	1.4733578140371861
1102	21	1.1987041847350994
1102	22	1.5134255255449816
1102	23	1.659310941340749
1102	24	1.5115335692540697
1102	25	1.307494613906961
1103	1	0.3197947023549723
1103	2	0.5428653898574076
1103	3	0.5493346663452918
1103	4	0.6092378188816809
1103	5	0.2417691865307745
1103	6	0.6460806264635934
1103	7	0.5300150459673816
1103	8	0.5769838260563631
1103	9	0.7633008589587433
1103	10	0.7229144666179721
1103	11	0.8084351540152994
1103	12	0.935470295872527
1103	13	0.8539205705282882
1103	14	1.0004764915061628
1103	15	1.0310791798159613
1103	16	1.12215273964474
1103	17	0.9987164424540111
1103	18	1.3330926459521137
1103	19	1.3306568166299826
1103	20	1.1278828319527037
1103	21	0.9143423118116729
1103	22	1.1029002149126725
1103	23	1.3986574332459654
1103	24	1.2511567142283084
1103	25	1.15437401190988
1104	1	0.43409356789909026
1104	2	0.4530648740121457
1104	3	0.5109235890992601
1104	4	0.6540418494602989
1104	5	0.39571608729067975
1104	6	0.4184401665732338
1104	7	0.6069379473731829
1104	8	0.6208929818270619
1104	9	0.6475136069536731
1104	10	0.6703394088508503
1104	11	0.7094574543622088
1104	12	0.6438026272947681
1104	13	0.6819913110093268
1104	14	0.8684731110435138
1104	15	0.9279846348049929
1104	16	0.7618647509059215
1104	17	0.8584675238597939
1104	18	1.1136856135016018
1104	19	0.9644207230827817
1104	20	1.0231281568307864
1104	21	0.9824743429238652
1104	22	0.9960265197738849
1104	23	1.2275514374882235
1104	24	1.0328110347175823
1104	25	0.8289386632579863
1105	1	0.5667834938093534
1105	2	0.8359341404972334
1105	3	0.6363791185095948
1105	4	0.8529975631052835
1105	5	0.5981543795594746
1105	6	0.4651647703599906
1105	7	0.7296789848034848
1105	8	0.7886602710632786
1105	9	0.6296856094646586
1105	10	0.8395319390603291
1105	11	0.8233982095458998
1105	12	0.8077158234113497
1105	13	0.8329632303428269
1105	14	0.8986990779926458
1105	15	0.9785104322234446
1105	16	0.9817165888368606
1105	17	1.0053034514866823
1105	18	1.012505293677215
1105	19	0.9621888084214874
1105	20	1.0269474041528568
1105	21	0.8978561741249038
1105	22	0.6727586191048545
1105	23	0.8939091902866494
1105	24	0.9300674828214852
1105	25	0.9625535906582243
1106	1	0.7122657731740993
1106	2	0.8105923511657913
1106	3	0.7727213322054176
1106	4	0.7517469593149428
1106	5	0.73219642096727
1106	6	0.7847987609692169
1106	7	0.5852992705943194
1106	8	0.6962368375447525
1106	9	0.7272643857698782
1106	10	0.8617248273390743
1106	11	0.8016785755552931
1106	12	0.9019294097206156
1106	13	0.8449600309873944
1106	14	0.9882516962426635
1106	15	1.1497357892664297
1106	16	1.1546268875932952
1106	17	1.1257529257246404
1106	18	1.257649059334213
1106	19	0.914310728112973
1106	20	1.1049741293309694
1106	21	0.9869425782332186
1106	22	1.0201010616054376
1106	23	1.300277002737154
1106	24	1.1651532259382762
1106	25	1.2278149658726107
1107	1	0.5341363069662179
1107	2	0.590516867131285
1107	3	0.7371327787230868
1107	4	0.819815867601605
1107	5	0.7055149665300178
1107	6	0.5852017000492609
1107	7	0.7806979072163571
1107	8	0.7414859682737074
1107	9	0.8798972921350625
1107	10	0.7092791305739325
1107	11	0.725734732475928
1107	12	0.906346245218586
1107	13	0.8778065469967302
1107	14	1.0902660298235247
1107	15	1.2340102467129626
1107	16	1.0682114810690138
1107	17	1.2200811942252585
1107	18	1.483052099510843
1107	19	1.4394650281127426
1107	20	1.2727734396181063
1107	21	1.2333250975048884
1107	22	1.1814089918134556
1107	23	1.2400531046527306
1107	24	1.2464903911516032
1107	25	1.2534792822031946
1108	1	0.5291067349122542
1108	2	0.602486911434184
1108	3	0.47671430288997385
1108	4	0.6870369984554543
1108	5	0.5760210075955996
1108	6	0.7200229801560525
1108	7	0.6667383631736001
1108	8	0.6660516789857596
1108	9	0.7952059146459831
1108	10	0.7533692078806524
1108	11	0.6552176761224331
1108	12	0.6465607700529077
1108	13	0.6625096755276942
1108	14	0.8901224526928573
1108	15	0.8234745862949495
1108	16	0.8350844321256048
1108	17	0.9201556735479457
1108	18	1.0961249759409668
1108	19	0.9423417210037854
1108	20	0.8598765975792304
1108	21	0.8431285355780531
1108	22	0.7311949189422862
1108	23	0.9722194901562773
1108	24	0.9240847699913175
1108	25	1.0826129529322799
1109	1	0.07264094067882709
1109	2	-0.08656383864560979
1109	3	0.19227468165367512
1109	4	0.26066014985453734
1109	5	0.1565678742835459
1109	6	0.022596445418315625
1109	7	0.15665154542703164
1109	8	0.23573973090617528
1109	9	0.42946381442255444
1109	10	0.5338335125939153
1109	11	0.011965628032207576
1109	12	0.328815265211972
1109	13	0.28829603577878515
1109	14	0.1492755540866213
1109	15	0.4679148458970346
1109	16	0.3171135841630601
1109	17	0.14497342011672743
1109	18	-1.4549102405714294
1109	19	-0.018475387199217153
1109	20	0.27814407284255793
1109	21	0.27397415745272014
1109	22	0.5169472330182501
1109	23	-0.00909852239825426
1109	24	0.28228308896142096
1109	25	0.46880978238221616
1110	1	0.3244084875370099
1110	2	0.3030041657948317
1110	3	0.3562448169653507
1110	4	0.15446231321681836
1110	5	0.24487448147353774
1110	6	0.21854471019327804
1110	7	0.3175463691969398
1110	8	0.1786573593304297
1110	9	0.4786310620923402
1110	10	0.5770667441670909
1110	11	0.4920105401371515
1110	12	0.026623304038771778
1110	13	0.2594494002944927
1110	14	0.43184935276396885
1110	15	0.5205127759465142
1110	16	0.6106441426706368
1110	17	0.24085111839673345
1110	18	0.6577433303978211
1110	19	0.5287239521494698
1110	20	0.47672952768338206
1110	21	0.5052055004837328
1110	22	0.595960385123739
1110	23	0.5280945894995597
1110	24	0.7176968083064565
1110	25	0.7300970370917312
1111	1	0.2895101218319631
1111	2	0.5142127405012609
1111	3	0.46721166704906025
1111	4	0.44418981201182817
1111	5	0.4239035306116534
1111	6	0.5408424495096469
1111	7	0.4016179405694942
1111	8	0.2969921252852572
1111	9	0.5312779138633843
1111	10	0.4950569721440843
1111	11	0.41161462832947393
1111	12	0.41328113178811066
1111	13	0.5221403751939988
1111	14	0.45124048401860506
1111	15	0.6396282802765327
1111	16	0.4277074253539439
1111	17	0.49318192326262533
1111	18	0.7863889948103306
1111	19	0.4977706837324476
1111	20	0.4421561814967916
1111	21	0.6219682438420975
1111	22	0.45653773043346035
1111	23	0.7158951893334589
1111	24	0.8139918796966441
1111	25	0.2773394885134124
1112	1	-0.4205703894153707
1112	2	-0.11195654607399419
1112	3	-0.01992910931045344
1112	4	0.010304677697654085
1112	5	-0.10871333620593049
1112	6	-0.03042163638760087
1112	7	-0.437847055106495
1112	8	-0.0403563331103598
1112	9	0.14400014897134383
1112	10	0.1627037002494518
1112	11	-0.0024986657508847543
1112	12	0.09036872841192434
1112	13	-0.0670705323611287
1112	14	0.016277984716481342
1112	15	-0.027769912237822325
1112	16	0.14064150145173215
1112	17	0.24136520081952284
1112	18	0.15208658025679256
1112	19	0.11802916074710623
1112	20	0.05012780271270145
1112	21	0.07129002082933056
1112	22	-0.011190312838465388
1112	23	0.25914769844548147
1112	24	-0.0777714147800097
1112	25	0.10391690998474701
1113	1	-0.4255546175522049
1113	2	-0.3219642643330545
1113	3	-0.4574352919021081
1113	4	-0.17718088383497133
1113	5	-0.4904065199718275
1113	6	-0.3786479378723797
1113	7	-0.4161855108798065
1113	8	-0.2001760345787389
1113	9	-0.38129468320176585
1113	10	-0.5884041413952872
1113	11	-0.48181009058409074
1113	12	-0.3243546012769727
1113	13	-0.40441632420660056
1113	14	-0.36692550798204593
1113	15	-0.6657572457762626
1113	16	-0.716384147653855
1113	17	-0.652218977707534
1113	18	-0.6364590548814064
1113	19	-0.7530588369874494
1113	20	-0.7670759731660866
1113	21	-0.5417653132328937
1113	22	-0.5536611990330016
1113	23	-0.9845686060307841
1113	24	-0.4053893897004599
1113	25	-0.37193839554652897
1114	1	-1.4123647949251463
1114	2	-1.2547424695212008
1114	3	-1.1651754764583764
1114	4	-1.3409660725779737
1114	5	-1.3254453056918791
1114	6	-1.8795869985991303
1114	7	-1.9689256741836303
1114	8	-1.4892988184795861
1114	9	-1.6479744980504236
1114	10	-1.7984722342429933
1114	11	-2.450757881817033
1114	12	-1.9716973650304936
1114	13	-1.7412314321477922
1114	14	-2.066373624377462
1114	15	-1.9428808542721399
1114	16	-2.6820696454074735
1114	17	-4.570341833866706
1114	18	-3.520065680545013
1114	19	-2.5130794943682133
1114	20	-1.7893479875157396
1114	21	-2.812851313394411
1114	22	-2.823224971257755
1114	23	-2.707704617456102
1114	24	-2.2289598862001796
1114	25	-2.2242647736616092
1115	1	-1.099823545582229
1115	2	-0.8039297782860793
1115	3	-0.8667433896889349
1115	4	-0.801145737925296
1115	5	-0.595046889319562
1115	6	-0.66915790651246
1115	7	-1.647904913583492
1115	8	-1.1011630883356283
1115	9	-0.9170305013602658
1115	10	-0.8128941672746208
1115	11	-1.4475854895434352
1115	12	-1.0757267978195983
1115	13	-0.9433792874943379
1115	14	-0.9164184769367125
1115	15	-2.3572169782578385
1115	16	-0.9998625229008908
1115	17	-1.0594201185465693
1115	18	-1.1668843594102327
1115	19	-1.1450690615709505
1115	20	-1.0126380238264474
1115	21	-1.7450783501249134
1115	22	-1.6921169230130957
1115	23	-1.1223706494129218
1115	24	-1.0534125747766012
1115	25	-1.2233532749023652
1116	1	-1.1060851310582365
1116	2	-1.9102399457812211
1116	3	-1.4063210189850395
1116	4	-1.056061148428956
1116	5	-1.5586204571222027
1116	6	-1.451309559665038
1116	7	-0.9961583374024912
1116	8	-1.6191365368284103
1116	9	-1.6494948518868426
1116	10	-2.6147330388170182
1116	11	-1.1725869505829607
1116	12	-1.1746592285716702
1116	13	-1.8555497532492922
1116	14	-1.4809325482858071
1116	15	-1.0822453250432886
1116	16	-1.0541229337840505
1116	17	-1.4692308145255666
1116	18	-1.3381069306573536
1116	19	-1.7125483775504406
1116	20	-2.0166791783963944
1116	21	-2.475704387773952
1116	22	-1.5564389227678959
1116	23	-1.5673558009304642
1116	24	-2.167955498110018
1116	25	-1.7852636188832278
1201	1	0.2279981392472692
1201	2	0.18145609262893103
1201	3	0.38348210150739703
1201	4	0.486060491253383
1201	5	0.5021098560904633
1201	6	0.5371263980414227
1201	7	0.4668513930009155
1201	8	-0.05562702326437119
1201	9	0.18972628149717963
1201	10	0.4855607857263635
1201	11	0.07972906448344474
1201	12	-0.4224259825428689
1201	13	0.03289872507463798
1201	14	0.5548854591099968
1201	15	0.2341745229497718
1201	16	0.6519527567834018
1201	17	0.6283159643849316
1201	18	1.0222347558853357
1201	19	0.4951765553423755
1201	20	0.5125504800876968
1201	21	0.42931620254767466
1201	22	0.6887254485630407
1201	23	0.9683932211871493
1201	24	0.8108868300866092
1201	25	0.6999867170128056
1202	1	0.5592515948262822
1202	2	0.6787313544385256
1202	3	0.2845343620781904
1202	4	0.6318469697254088
1202	5	0.6450642754762725
1202	6	0.6249679178356118
1202	7	0.634140735022477
1202	8	0.6999830725009772
1202	9	0.9073282657573856
1202	10	1.0538992664208209
1202	11	0.7193659922825404
1202	12	0.7526236737316481
1202	13	0.7001020113020004
1202	14	0.8785615255548436
1202	15	0.5322233820091995
1202	16	0.7280281601190381
1202	17	0.9920938499461087
1202	18	1.2817392156748397
1202	19	1.0041568982444034
1202	20	0.8886514015452676
1202	21	0.9304986779321922
1202	22	1.058622434981423
1202	23	1.2448318505563378
1202	24	1.2240446343336586
1202	25	1.1954280221652098
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2211	12	0.26466094276447905
2211	13	0.08449559289184805
2211	14	0.354732523106037
2211	15	0.27538265059678935
2211	16	0.14123243265702712
2211	17	0.31959449379505855
2211	18	0.2493440539268832
2211	19	0.11450592985192998
2211	20	-0.03443348611945396
2211	21	0.09574764013498083
2211	22	0.006753308739014585
2211	23	0.6083847618448601
2211	24	0.36331527351197934
2211	25	0.3853673039353325
2212	1	-0.1785441106960377
2212	2	-0.20521685288660052
2212	3	-0.1642655116217533
2212	4	-0.27410429996244545
2212	5	-0.23354254941477137
2212	6	-0.3162039701985577
2212	7	-0.3411323405907183
2212	8	-0.24932420753906115
2212	9	-0.1531001410005004
2212	10	-0.09424235785857604
2212	11	-0.3708827057651831
2212	12	-0.19839558564812876
2212	13	-0.39975328137356314
2212	14	-0.4031208642738662
2212	15	-0.42994985451247203
2212	16	-0.34359041784711764
2212	17	-0.330387697974146
2212	18	-2.7205880627933468
2212	19	-1.1947777552646315
2212	20	-0.5722117821261747
2212	21	-0.635032402774371
2212	22	-0.3120882234472404
2212	23	-0.47193544710504653
2212	24	-0.8398666757260855
2212	25	-0.5539184164076048
2213	1	0.017131575656197384
2213	2	0.1020045656484676
2213	3	-0.18309324536701155
2213	4	-0.23442258731873977
2213	5	-0.2537891655853599
2213	6	-0.2632937713404786
2213	7	-0.06206908904958652
2213	8	-0.47091403694624745
2213	9	-0.35410936433221707
2213	10	-0.2693644263585995
2213	11	-0.4055728741512681
2213	12	-0.3400726048950702
2213	13	-0.15599360562727327
2213	14	-0.13705895516270772
2213	15	-0.3615670603646777
2213	16	-0.9374111023025122
2213	17	-0.5116080832944618
2213	18	-0.3042809269368476
2213	19	-0.2545506251020484
2213	20	-1.0378103962425982
2213	21	-0.572917176512604
2213	22	-0.4938892386811986
2213	23	-0.8560396730691835
2213	24	-0.6481257361068273
2213	25	-0.8407807536524388
2214	1	-1.630425846165977
2214	2	-3.7481909835829583
2214	3	-1.9059761519025997
2214	4	-1.3944488274163547
2214	5	-1.4347784021393366
2214	6	-1.539678691891794
2214	7	-1.7324492013172978
2214	8	-1.4424027807330866
2214	9	-1.5743816764734149
2214	10	-1.5741828120019647
2214	11	-1.3583228713151314
2214	12	-1.7825723613476399
2214	13	-1.6628180658208223
2214	14	-2.791759294580494
2214	15	-2.866371922597864
2214	16	-2.2216549969149924
2214	17	-2.646599177831746
2214	18	-1.9581703119242064
2214	19	-4.220928924404465
2214	20	-2.5644794254544223
2214	21	-2.289041709293187
2214	22	-2.4626821239628853
2214	23	-2.497184009272324
2214	24	-2.690330390500865
2214	25	-2.4929049824714227
2215	1	-0.8682978437240862
2215	2	-2.429812371808932
2215	3	-1.2480886634572137
2215	4	-0.7657716622189312
2215	5	-0.7357195694516321
2215	6	-1.3020982891492103
2215	7	-1.6789666243286767
2215	8	-1.676858188422493
2215	9	-1.3984515318455522
2215	10	-2.884336243113374
2215	11	-1.9890700198269826
2215	12	-2.205197353467643
2215	13	-1.412529241225883
2215	14	-2.7174801362396224
2215	15	-2.5819910408259403
2215	16	-2.3040013635076377
2215	17	-2.5102697787703434
2215	18	-1.434022182689084
2215	19	-2.017306025267594
2215	20	-2.1919526388181154
2215	21	-1.8940856803759942
2215	22	-1.5057072346390186
2215	23	-2.170739772229716
2215	24	-2.000591464469295
2215	25	-1.9323297561769905
2216	1	-1.924213088039938
2216	2	-1.9117714414914104
2216	3	-2.4439281466392586
2216	4	-3.1256317212593316
2216	5	-2.248367437937919
2216	6	-2.2395334731481427
2216	7	-2.089824966737986
2216	8	-2.684144861738549
2216	9	-2.92153503704116
2216	10	-2.3797858414035424
2216	11	-2.9117793326085426
2216	12	-3.343859663294772
2216	13	-3.9057348800315452
2216	14	-3.277689505760513
2216	15	-3.2220376050579667
2216	16	-2.296574717735865
2216	17	-2.3950662874441377
2216	18	-2.4647980126789655
2216	19	-2.7057102012452994
2216	20	-2.6961353233453025
2216	21	-3.0383343826201177
2216	22	-3.6036162999722485
2216	23	-3.452882047422232
2216	24	-3.3378017383919634
2216	25	-3.4107368047859694
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	141453.0
3	5.0
4	1233.0
5	7179.0
6	8706.0
7	7926.0
8	6947.0
9	6587.0
10	6075.0
11	5740.0
12	5608.0
13	5468.0
14	5683.0
15	5690.0
16	5972.0
17	6090.0
18	7054.0
19	8513.0
20	10739.0
21	13809.0
22	17160.0
23	21074.0
24	24199.0
25	27837.0
26	31118.0
27	34701.0
28	39196.0
29	46584.0
30	57143.0
31	71693.0
32	90472.0
33	115090.0
34	149265.0
35	196587.0
36	268129.0
37	413404.0
38	1186170.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.556092287899425	19.0668956909525	13.334874464065768	25.042137557082313
2	14.640113960039358	20.205714629868815	41.448840072979465	23.705331337112362
3	18.231086869998745	24.64431506347145	29.925681584900914	27.198916481628892
4	12.117873593522472	17.052348441480355	38.78469082124181	32.04508714375536
5	13.287912238982821	37.73936496662155	35.95206727079013	13.020655523605495
6	31.584724167078726	37.30902181799023	18.383647257444167	12.722606757486876
7	27.71135750642877	31.6774625514678	22.662326059937286	17.94885388216614
8	24.69586828573523	36.76239959366197	20.972553855498653	17.56917826510415
9	26.121499394898564	15.375303614311155	20.48328918612711	38.01990780466318
10	14.628295635577745	29.156679892188652	34.84367616277472	21.371348309458888
11	33.82225119513255	22.69779901876083	24.94960284423221	18.530346941874406
12	22.677388455510652	24.8760598604121	32.07504379507341	20.37150788900383
13	28.39292487300043	22.170101373850148	26.17073101289746	23.266242740251965
14	20.880640768304055	22.06030063587869	28.055508554039545	29.00355004177771
15	22.883404239720864	30.728828642187683	23.671802102285206	22.71596501580625
16	22.31381861165909	28.26903114073612	27.470615340108047	21.946534907496737
17	21.63486083366278	28.596973973237542	27.870150254088855	21.89801493901082
18	21.508111172871633	27.95240501917615	29.34601283781814	21.193470970134072
19	23.179415246324467	27.330068665373062	27.647491787875346	21.843024300427125
20	23.284998416035478	27.66102766570756	27.951868423601855	21.102105494655103
21	23.0054507996197	27.034297000669284	27.316573474520663	22.64367872519035
22	22.937452118532995	27.917811922088486	27.39894944666123	21.745786512717284
23	22.536752637998504	27.62476882843511	27.481557818990577	22.35692071457581
24	22.82261487475542	27.106158538959946	28.10009094357146	21.971135642713175
25	22.781206064620516	27.3018866195579	27.80983524150877	22.107072074312807
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	236.0
1	236.0
2	487.5
3	739.0
4	739.0
5	739.0
6	1463.5
7	2188.0
8	2188.0
9	2188.0
10	3556.0
11	4924.0
12	4924.0
13	4924.0
14	9170.0
15	13416.0
16	13416.0
17	13416.0
18	24945.5
19	36475.0
20	36475.0
21	36475.0
22	58149.0
23	79823.0
24	79823.0
25	79823.0
26	113855.0
27	147887.0
28	147887.0
29	147887.0
30	190272.0
31	232657.0
32	232657.0
33	232657.0
34	264190.5
35	295724.0
36	295724.0
37	295724.0
38	322185.0
39	348646.0
40	348646.0
41	348646.0
42	366436.5
43	384227.0
44	384227.0
45	384227.0
46	389173.5
47	394120.0
48	394120.0
49	394120.0
50	381161.0
51	368202.0
52	368202.0
53	368202.0
54	330201.0
55	292200.0
56	292200.0
57	292200.0
58	250100.5
59	208001.0
60	208001.0
61	208001.0
62	168697.5
63	129394.0
64	129394.0
65	129394.0
66	99136.0
67	68878.0
68	68878.0
69	68878.0
70	49887.0
71	30896.0
72	30896.0
73	30896.0
74	21413.0
75	11930.0
76	11930.0
77	11930.0
78	8004.5
79	4079.0
80	4079.0
81	4079.0
82	2636.5
83	1194.0
84	1194.0
85	1194.0
86	764.5
87	335.0
88	335.0
89	335.0
90	210.5
91	86.0
92	86.0
93	86.0
94	52.5
95	19.0
96	19.0
97	19.0
98	21.0
99	23.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3571640078408559
2	0.03903413900276118
3	0.03491150571328264
4	0.034682470530533825
5	0.03873966519636986
6	0.016948603523411813
7	0.016523252469735454
8	0.023394307952199704
9	0.020056938146431353
10	0.01933711328636367
11	0.018682727049938504
12	0.01930439397454241
13	0.01897720085632983
14	0.02754966055349951
15	0.028204046789924674
16	0.02038413126464394
17	0.022085535479349373
18	0.10758109726829738
19	0.024735799736871292
20	0.022085535479349373
21	0.02565194046786653
22	0.018846323609044795
23	0.02185650029660056
24	0.018257375996262145
25	0.022085535479349373
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3056299.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.44909299327217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.59259545168231	55.9708265609305
2	12.685122926957945	17.619405635707828
3	3.578302180047218	7.455295225803835
4	1.3458654287970173	3.7387653328381685
5	0.6259105133711765	2.1734458724290784
6	0.32681421105854935	1.3618170321196847
7	0.1907647404861567	0.927390674130237
8	0.13317619257253208	0.7399172625967763
9	0.09347465878846738	0.5842557243643192
>10	0.39222744470968335	4.637591159038073
>50	0.021003524382534233	1.0035890293166223
>100	0.01196207690796711	1.5744545569893267
>500	0.0017909272736194286	0.8571299683144341
>1k	9.897229647605828E-4	1.3561159654211765
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4174	0.13657040754193225	No Hit
GTCCTACAGTGGACATTTCTAAATT	3743	0.12246838414696991	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3344	0.10941337873028785	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3299	0.10794100969833123	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3186	0.10424372746252902	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	3.2719311821258325E-5	0.0	0.0
10	0.0	0.0	3.2719311821258325E-5	0.0	0.0
11	0.0	0.0	3.2719311821258325E-5	0.0	0.0
12	0.0	0.0	3.2719311821258325E-5	0.0	0.0
13	3.2719311821258325E-5	0.0	3.2719311821258325E-5	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1245	0.0	12.321185	1
TAGGACC	715	0.0	12.087284	4
GGACGTG	1780	0.0	10.561309	6
AGGACGT	1865	0.0	10.540903	5
GTCCTAC	2155	0.0	10.47845	1
GTAGGAC	1970	0.0	10.220285	3
GTATCAA	2580	0.0	10.192609	1
TCCAACG	245	0.0	10.084223	18
GGCGAGG	935	0.0	9.951629	19
TAGGACG	1990	0.0	9.831223	4
TCCTACA	2440	0.0	9.730689	2
GTCCTAA	1200	0.0	9.686671	1
TTAGGAC	1240	0.0	9.650332	3
GACGTGG	950	0.0	9.594452	7
TGTAGGA	2300	0.0	9.579736	2
GATATAC	680	0.0	9.527873	1
AAGCCGT	80	0.004539148	9.49451	6
AATCCCG	375	0.0	9.368071	19
GCTCTAA	265	0.0	9.348103	1
TGGCGAG	2035	0.0	9.339007	18
>>END_MODULE