FastQCFastQC Report
Thu 26 May 2016
SRR1512991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512991_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3056299
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52020.1702058600941858No Hit
TATCAACGCAGAGTACTTTTTTTTT39530.12933943962943414No Hit
GTCCTACAGTGGACATTTCTAAATT35150.11500838105172302No Hit
CTTTAGGACGTGAAATATGGCGAGG33010.10800644832197374No Hit
CTGTAGGACGTGGAATATGGCAAGA32380.10594513167723446No Hit
GGTATCAACGCAGAGTACTTTTTTT31000.10142986664590081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13400.012.8424751
AACACCG1209.780706E-911.8949445
GTCCTAA10100.011.2021271
GGGCGAA600.0059149611.07396811
TAGGACC7950.011.0121764
TAGTACG700.001471371210.8753794
GTCCTAC20050.010.4323761
TCCAACG2300.010.31432318
TGGCGAG21150.010.13973518
AGGACCT15200.010.0167965
TTAGGAC14000.09.9829093
GGCGAGG9900.09.87290219
GACAGCG1451.6062404E-79.8327997
GTATTAA3950.09.6280391
GGACGTG18950.09.5887636
TCCTACA23900.09.5867792
AGGACGT19300.09.5652625
GGCGAGA12250.09.5282219
GTAGGAC20100.09.4602313
TAGGACA7650.09.453764
Produced by FastQC (version 0.11.5)