##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512991_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3056299
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.572607588459114	31.0	31.0	34.0	27.0	34.0
2	30.726916770904943	31.0	31.0	34.0	27.0	34.0
3	30.823625568048154	31.0	31.0	34.0	27.0	34.0
4	34.29384919472866	37.0	35.0	37.0	30.0	37.0
5	33.953726386063664	37.0	35.0	37.0	30.0	37.0
6	33.827710901322156	37.0	35.0	37.0	29.0	37.0
7	33.70333956199966	37.0	35.0	37.0	28.0	37.0
8	33.594480775604744	37.0	35.0	37.0	28.0	37.0
9	35.06633938629695	39.0	35.0	39.0	28.0	39.0
10	34.96436343433676	39.0	35.0	39.0	28.0	39.0
11	34.98975885539995	39.0	35.0	39.0	28.0	39.0
12	34.84615575897515	39.0	35.0	39.0	27.0	39.0
13	34.82829101472075	39.0	35.0	39.0	27.0	39.0
14	35.89483293355787	40.0	36.0	41.0	27.0	41.0
15	35.908486702380884	40.0	36.0	41.0	27.0	41.0
16	35.885030554929344	40.0	36.0	41.0	27.0	41.0
17	35.86421125681748	40.0	36.0	41.0	27.0	41.0
18	35.78964230921124	40.0	36.0	41.0	27.0	41.0
19	35.77789149556375	40.0	36.0	41.0	27.0	41.0
20	35.74602975690533	40.0	36.0	41.0	27.0	41.0
21	35.69518198317638	40.0	36.0	41.0	27.0	41.0
22	35.65522548677338	40.0	36.0	41.0	27.0	41.0
23	35.54687712164288	40.0	36.0	41.0	27.0	41.0
24	35.52905687565254	40.0	36.0	41.0	27.0	41.0
25	35.43141197899813	40.0	36.0	41.0	26.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4790428648029348
1101	2	0.5467449485906002
1101	3	0.5443259880842994
1101	4	0.37696019255300683
1101	5	0.5536863795298999
1101	6	0.6585472268441634
1101	7	0.5869242826189875
1101	8	0.538049446010632
1101	9	0.536122223600735
1101	10	0.8819246060178259
1101	11	0.7886776176032342
1101	12	0.8294445201185994
1101	13	0.9077500616718126
1101	14	0.9657280252751335
1101	15	0.8606599967496606
1101	16	1.0482675020109795
1101	17	0.9136887325239442
1101	18	0.43034692678624253
1101	19	0.7662477060523614
1101	20	0.8001296537365263
1101	21	0.950582467392735
1101	22	0.8948658825133791
1101	23	0.966530209113067
1101	24	0.8906943873714752
1101	25	0.9253239811255156
1102	1	0.7316910504110901
1102	2	0.6800688450467476
1102	3	0.6223318237273858
1102	4	0.5832259745885437
1102	5	0.8512119390073423
1102	6	0.8152027366866719
1102	7	0.8160088903112594
1102	8	0.7479828247018574
1102	9	0.9548912590757865
1102	10	0.9047206005200792
1102	11	0.8914022875788916
1102	12	0.9561839116226736
1102	13	1.0771627073916505
1102	14	1.1430722140613838
1102	15	1.152036669702511
1102	16	1.0538675275818719
1102	17	0.9353938544651399
1102	18	1.1280304778526826
1102	19	1.1260278876970276
1102	20	1.1201210692224421
1102	21	1.0652062784603658
1102	22	1.0736004886074682
1102	23	1.0761581069573438
1102	24	1.1842057732682107
1102	25	1.2067261793090651
1103	1	0.5507414699867041
1103	2	0.4683557110761605
1103	3	0.5477377674847084
1103	4	0.49525654564509836
1103	5	0.6185659844337792
1103	6	0.6680911285916267
1103	7	0.6243611357078862
1103	8	0.6881346447667056
1103	9	0.7875024981513334
1103	10	0.8013359733279444
1103	11	0.8608174876124508
1103	12	0.8737998404139589
1103	13	0.8704996520887036
1103	14	0.9976033004958325
1103	15	0.8421181217206026
1103	16	0.941717727976858
1103	17	1.0567107766194326
1103	18	1.0558105511568243
1103	19	1.0660579804115002
1103	20	1.192144036781741
1103	21	0.7661468988950304
1103	22	0.9831157478090802
1103	23	1.0329259780762854
1103	24	1.037180998539668
1103	25	1.034702408563433
1104	1	0.42849123325778393
1104	2	0.46531452331568346
1104	3	0.4335030535032658
1104	4	0.3846830341851373
1104	5	0.43442081058830695
1104	6	0.48211583615791653
1104	7	0.5508798647171176
1104	8	0.3746758048832106
1104	9	0.5703009279738254
1104	10	0.5730441688436514
1104	11	0.6331678130653486
1104	12	0.5741969482403633
1104	13	0.6749390981913024
1104	14	0.6664990072556165
1104	15	0.8445645692071508
1104	16	0.7491570275225357
1104	17	0.7285322685337903
1104	18	0.6790117404618528
1104	19	0.7207525847473022
1104	20	0.6888213193180377
1104	21	0.4536816622845876
1104	22	0.5353121270632926
1104	23	0.4631979684158125
1104	24	0.5357579922622904
1104	25	0.6779348574944848
1105	1	0.45918067976643684
1105	2	0.5392510848228653
1105	3	0.49364629104763225
1105	4	0.41527351166826776
1105	5	0.3468294817540425
1105	6	0.5091312479190861
1105	7	0.6018080636679173
1105	8	0.6486557387501506
1105	9	0.7591309139789502
1105	10	0.666412852607813
1105	11	0.7220512640956969
1105	12	0.7298172360640365
1105	13	0.7292000518872825
1105	14	0.9025576212803372
1105	15	0.8171520197607052
1105	16	0.9027657862160368
1105	17	0.6041576983965058
1105	18	0.9344825959327068
1105	19	0.9026094524798793
1105	20	0.9260688667350792
1105	21	0.8387147614419845
1105	22	0.9135220623353746
1105	23	0.8483683626257559
1105	24	0.8608492721558321
1105	25	0.8386736928095928
1106	1	0.5194681915244601
1106	2	0.5872539321709809
1106	3	0.45132288627636896
1106	4	0.47704923215881223
1106	5	0.5407886449935262
1106	6	0.6219977022080059
1106	7	0.6191682825382472
1106	8	0.6750425987079467
1106	9	0.6527241851634358
1106	10	0.7941003796194863
1106	11	0.7118743413045863
1106	12	0.785609223204041
1106	13	0.6843949954976623
1106	14	0.9220001590184523
1106	15	0.891769358468018
1106	16	0.965339781088467
1106	17	0.8618986327412728
1106	18	1.0060644581687725
1106	19	1.0290735584888395
1106	20	1.1166374172275937
1106	21	0.9872939229996547
1106	22	1.0753159698708714
1106	23	1.170995197708372
1106	24	1.106628215468966
1106	25	1.1621484744183874
1107	1	0.524975337776322
1107	2	0.44340591503691584
1107	3	0.44528301420718464
1107	4	0.4426983563324569
1107	5	0.4626281261700882
1107	6	0.46197448617438397
1107	7	0.4958604957809598
1107	8	0.5691157207004593
1107	9	0.4454348018164467
1107	10	0.6248830779278975
1107	11	0.6494032469478412
1107	12	0.5526170004050357
1107	13	0.555840104199909
1107	14	0.8095375461334697
1107	15	0.7385626491449742
1107	16	0.7486436899934148
1107	17	0.6653625211459655
1107	18	0.71427960958912
1107	19	0.8809091213744296
1107	20	0.8455124973865509
1107	21	0.7713561627424426
1107	22	0.8197629044839445
1107	23	0.7986360694206311
1107	24	0.792657709707207
1107	25	0.9130121691858619
1108	1	0.5543789671455173
1108	2	0.47388832788948676
1108	3	0.46456611656632774
1108	4	0.5544598879619898
1108	5	0.5763889325564335
1108	6	0.5947118167538932
1108	7	0.5477031495404034
1108	8	0.5901546683620751
1108	9	0.5705642682371632
1108	10	0.7506879664874475
1108	11	0.6316570715546064
1108	12	0.7262690203124293
1108	13	0.6861989734511766
1108	14	0.7506209753775863
1108	15	0.7442125248551079
1108	16	0.6716102499757568
1108	17	0.7160333080348238
1108	18	0.7210353024854115
1108	19	0.656414400409119
1108	20	0.7173646318613507
1108	21	0.8377759103788378
1108	22	0.9202435199946848
1108	23	0.9841349053527537
1108	24	0.9578342223385263
1108	25	0.9358530834127166
1109	1	0.07872113120552271
1109	2	0.07740508499130883
1109	3	0.08964516752919849
1109	4	0.21839392349768616
1109	5	0.07764119343524811
1109	6	-0.16579653519843873
1109	7	0.030883487874270088
1109	8	0.11150821661188814
1109	9	0.16464448953730226
1109	10	0.09125398414889929
1109	11	0.10348811741054931
1109	12	0.23351234407027732
1109	13	0.22889351542372793
1109	14	0.24128657810128118
1109	15	0.18908368136111875
1109	16	0.08169642935654053
1109	17	0.09498830324708507
1109	18	0.1193681038472647
1109	19	0.3516926214964684
1109	20	0.2894165522784178
1109	21	0.3635750379456013
1109	22	0.38930320719545364
1109	23	0.30740695557086184
1109	24	-0.17223365722313133
1109	25	0.04971434524907892
1110	1	0.21907365907674858
1110	2	0.2209831955014856
1110	3	0.1587246893692722
1110	4	0.24352744139822136
1110	5	0.20755072617335912
1110	6	0.2513626303231433
1110	7	0.18986929334765534
1110	8	0.277204946523284
1110	9	0.33235600287555656
1110	10	0.3998896647348218
1110	11	0.25002595932806315
1110	12	0.38770876599361515
1110	13	0.041286311887617444
1110	14	0.3327052184862964
1110	15	0.3790816845154552
1110	16	0.4112890051520637
1110	17	0.25862811290369336
1110	18	0.20590124133178023
1110	19	0.38841799768188423
1110	20	0.30753437294136887
1110	21	0.307199008216287
1110	22	0.3278137295567376
1110	23	0.4415464166255987
1110	24	0.3930406890637528
1110	25	0.42209983122716466
1111	1	-0.23746777365077776
1111	2	-0.044677818130665514
1111	3	-0.033212907574714734
1111	4	-0.060224908942387856
1111	5	0.21925237969287537
1111	6	0.040620752526329795
1111	7	0.2609093972985761
1111	8	0.3276439130441986
1111	9	-0.1088789986838492
1111	10	-0.04880747377950456
1111	11	0.10703733916039937
1111	12	0.03810497861723405
1111	13	0.17927257047729483
1111	14	0.3356003125587037
1111	15	0.4796312268257381
1111	16	0.18444163224334176
1111	17	0.4538975921186932
1111	18	0.4081845726020461
1111	19	0.3877290600323846
1111	20	0.3013700665848873
1111	21	0.3674349793019971
1111	22	0.1847863836592083
1111	23	0.36595266820315686
1111	24	0.3379584383796512
1111	25	0.39633468687354423
1112	1	-0.10312655671217641
1112	2	-0.36020918914043065
1112	3	-0.13248247336165164
1112	4	-0.32173303876062675
1112	5	-0.1318342133819428
1112	6	-0.11676324554426287
1112	7	-0.1202893272657164
1112	8	0.02826809578801459
1112	9	-0.27533395791172666
1112	10	-0.017628429122972022
1112	11	-0.23030454329790473
1112	12	0.02388488434703362
1112	13	-0.02388077872596739
1112	14	-0.05724997129097886
1112	15	-0.1228243588146114
1112	16	-0.3509277216068014
1112	17	-0.15895219875343258
1112	18	-0.21302632659162413
1112	19	-0.15242461483219927
1112	20	0.05197180839825677
1112	21	0.20681718784666003
1112	22	-0.10533900596507095
1112	23	0.06757391111104738
1112	24	0.01860932204191812
1112	25	0.004454201355109433
1113	1	-0.3364492512681956
1113	2	-0.7101435573237431
1113	3	-0.3919047217490679
1113	4	-0.38231598555999824
1113	5	-0.21045310278301343
1113	6	-0.35548135449626983
1113	7	-0.08364320358833766
1113	8	-0.10329345173889237
1113	9	-0.14107649169375236
1113	10	-0.38174195247096776
1113	11	-0.20705689151168372
1113	12	-0.25443511147977915
1113	13	-0.08431676987285641
1113	14	-0.15552915083471675
1113	15	-0.1664461705082516
1113	16	-0.44882020040288495
1113	17	-0.20546210846577395
1113	18	-0.03429882797824746
1113	19	-0.22127923583374098
1113	20	0.028676974095972696
1113	21	-0.30859209812388855
1113	22	-0.4776711870599186
1113	23	-0.20019457518398553
1113	24	-0.45113933950550233
1113	25	-0.509533391942675
1114	1	-0.7914198591307517
1114	2	-0.6235646916094701
1114	3	-0.6470562304604464
1114	4	-0.4559628208577635
1114	5	-0.6189576104275218
1114	6	-1.0130417433310868
1114	7	-0.9043549056119957
1114	8	-1.2287875499337133
1114	9	-1.1073739324737986
1114	10	-1.0839511870852547
1114	11	-0.9667030807349946
1114	12	-0.898333533272897
1114	13	-0.8233386046393179
1114	14	-1.9660805243805157
1114	15	-1.3582570067071202
1114	16	-1.2450301235965284
1114	17	-1.4257951768518993
1114	18	-1.5702875556791156
1114	19	-1.483130834230458
1114	20	-0.7886734405393128
1114	21	-0.8518293987227779
1114	22	-0.9445147307783301
1114	23	-0.8802031186686179
1114	24	-1.6267584871122693
1114	25	-1.5755005271550075
1115	1	-1.5799490665724036
1115	2	-1.334917126863079
1115	3	-1.1123233509226722
1115	4	-2.282323066478675
1115	5	-1.4531346444941207
1115	6	-1.3566016742118094
1115	7	-1.0903777929991847
1115	8	-1.0413211199353753
1115	9	-1.5540926670988497
1115	10	-1.226533125656431
1115	11	-1.2404801868572406
1115	12	-1.7058901525787036
1115	13	-1.201303849936565
1115	14	-2.043073990258989
1115	15	-2.020345860416626
1115	16	-1.4547505903685831
1115	17	-1.3877867762599791
1115	18	-1.311848567047491
1115	19	-1.1520181176404947
1115	20	-1.2154970411247135
1115	21	-1.2406475923577602
1115	22	-1.457572821480639
1115	23	-1.9484213969585795
1115	24	-1.4074800171910766
1115	25	-1.2151284357380447
1116	1	-0.9677538668041095
1116	2	-0.9816375961555188
1116	3	-0.8278671834394906
1116	4	-0.9730846571703395
1116	5	-0.9463523877421451
1116	6	-1.577894320798805
1116	7	-0.9178255994081965
1116	8	-1.0693165139182668
1116	9	-1.1871112698963913
1116	10	-1.2429958876544234
1116	11	-1.0696190019352017
1116	12	-1.6948703574681687
1116	13	-1.3449241002978525
1116	14	-1.6577094642810977
1116	15	-1.5188449942024107
1116	16	-1.78783333923883
1116	17	-1.5464879660284296
1116	18	-2.5329925127637765
1116	19	-1.7995724378677949
1116	20	-1.5598447865999745
1116	21	-1.6329413083078492
1116	22	-2.9539643484727236
1116	23	-2.792357410803689
1116	24	-1.9808343487575257
1116	25	-2.3738053148563694
1201	1	0.5220088109858878
1201	2	0.613480825617323
1201	3	0.5314885875790267
1201	4	0.5367688471732066
1201	5	0.5740567284197127
1201	6	0.7019940883669946
1201	7	0.6374382565386654
1201	8	0.7093160883956742
1201	9	0.9703208024297894
1201	10	0.7923878205181296
1201	11	0.8962977942404464
1201	12	0.7631436244050818
1201	13	0.7841905457886114
1201	14	0.7991779722654115
1201	15	0.9356445114149139
1201	16	0.8992067136624442
1201	17	0.8757008284843053
1201	18	0.9852364647316918
1201	19	0.7771370862445863
1201	20	0.9380222098723152
1201	21	0.9162736932976685
1201	22	1.0625573264889425
1201	23	0.8904045354720012
1201	24	1.0496261196642536
1201	25	0.8972376437145684
1202	1	0.5100686826532623
1202	2	0.6383738440582398
1202	3	0.6617779196467097
1202	4	0.6400292396998353
1202	5	0.6275130765954842
1202	6	0.7441658162786595
1202	7	0.631286715090269
1202	8	0.5336269859910914
1202	9	0.7920441896075658
1202	10	0.7298297373815359
1202	11	0.8750692505017525
1202	12	0.826391285017678
1202	13	0.7944896869499729
1202	14	0.7750180116465657
1202	15	0.9734590200055635
1202	16	0.832811727520955
1202	17	0.9398270997527973
1202	18	0.9365497431135879
1202	19	1.0133363972552942
1202	20	1.0958583998176294
1202	21	1.0434344764687395
1202	22	1.0209756825246572
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2211	7	0.09293710729249227
2211	8	0.25608478660307554
2211	9	-0.029781154906189045
2211	10	-0.21676785920671904
2211	11	0.17283948154531004
2211	12	0.12276696105907803
2211	13	0.0800355617333608
2211	14	-1.0090006534253888
2211	15	-0.3909021290574728
2211	16	-0.02913108123189545
2211	17	0.17878659619744042
2211	18	-0.0780293979264357
2211	19	-0.0844916710668997
2211	20	-0.19722976165532202
2211	21	-0.1997838079684442
2211	22	-0.016945798458884553
2211	23	-0.1185365440959032
2211	24	-0.2210875260340046
2211	25	-0.26331577536236495
2212	1	-0.206360803721914
2212	2	-0.14668286687319565
2212	3	-0.07175085015489202
2212	4	0.07600762391398064
2212	5	-0.10254450988765029
2212	6	-0.05039511177856326
2212	7	-0.20619537746451044
2212	8	-0.27382023616602424
2212	9	-0.32124151463244743
2212	10	-0.37453843831342226
2212	11	-0.24188084443011348
2212	12	-0.22019306370284397
2212	13	-0.334989242588108
2212	14	-0.3611373443169015
2212	15	-0.2781562694711326
2212	16	-0.35232592023701415
2212	17	-0.37218764122867753
2212	18	-0.20191341546330221
2212	19	-0.200683253709812
2212	20	-0.4243582268402335
2212	21	-0.3147816455404211
2212	22	-0.3871001788596544
2212	23	-0.7582257056674351
2212	24	-0.4274281890089924
2212	25	-0.4689510627956892
2213	1	-0.23901672413781583
2213	2	-0.2923447781638444
2213	3	-0.22505749494492377
2213	4	-0.24523677326276072
2213	5	-0.11396223105315784
2213	6	0.04894377040157849
2213	7	-0.5090550053301115
2213	8	-0.18716624459928966
2213	9	-0.35822474549566863
2213	10	-0.3043816580203824
2213	11	-0.2450214392812029
2213	12	-0.4545920827958625
2213	13	-0.3161187703946595
2213	14	-0.17802667659590554
2213	15	-0.3668446792807458
2213	16	-0.5050119210284407
2213	17	-0.37544759724383425
2213	18	-0.5555298884617983
2213	19	-0.7182928565453324
2213	20	-0.26048113784958815
2213	21	-0.9251103535860778
2213	22	-0.3977496295939673
2213	23	-0.5155858283455146
2213	24	-0.6021513085683523
2213	25	-0.6840274505352326
2214	1	-1.027811759479654
2214	2	-0.9333437061949894
2214	3	-0.7493125684296231
2214	4	-1.3181611773998796
2214	5	-1.225003294140862
2214	6	-2.6731789620187527
2214	7	-1.312644219231025
2214	8	-1.868942936182524
2214	9	-1.3302261543793037
2214	10	-1.4798984152542758
2214	11	-1.3065000143999654
2214	12	-1.253566770609467
2214	13	-1.4763230117034354
2214	14	-1.6085227408513774
2214	15	-1.9805206464881877
2214	16	-1.424834845387558
2214	17	-1.6027485712714977
2214	18	-1.4810341757069523
2214	19	-3.402887045296481
2214	20	-1.8666390073668637
2214	21	-2.2547342029032365
2214	22	-1.927443328855297
2214	23	-1.9683558135057737
2214	24	-1.388566691906611
2214	25	-1.159098907622358
2215	1	-1.3930629225765543
2215	2	-1.2592189538969407
2215	3	-1.2389654919155717
2215	4	-1.130236210430688
2215	5	-1.7710994387666759
2215	6	-1.5443779516657656
2215	7	-1.6206640683503792
2215	8	-2.3175288992001235
2215	9	-1.8157322671620193
2215	10	-1.2708922093083501
2215	11	-1.8796974830794824
2215	12	-2.5661459372284128
2215	13	-2.090604853666406
2215	14	-1.9708929210982191
2215	15	-3.169011623064975
2215	16	-2.613569601375822
2215	17	-2.2394868455795773
2215	18	-3.021642024491463
2215	19	-3.593134853865763
2215	20	-3.0746099423056066
2215	21	-2.258946474617275
2215	22	-3.0102577463309856
2215	23	-3.0415197601882937
2215	24	-2.0668949163944887
2215	25	-2.479441349720325
2216	1	-1.144641132857796
2216	2	-1.0486189074494305
2216	3	-1.3473238577153808
2216	4	-0.8658934570715076
2216	5	-0.9527261595547856
2216	6	-1.4164685093054672
2216	7	-2.596973429154257
2216	8	-1.9365012253209386
2216	9	-1.8914446283046615
2216	10	-1.848662563883316
2216	11	-2.320917296250066
2216	12	-1.7895058949717395
2216	13	-2.3754243915609123
2216	14	-2.319383020339714
2216	15	-2.489991345030205
2216	16	-2.1255684645512147
2216	17	-2.3514971399932563
2216	18	-2.0802413402290227
2216	19	-2.2835840542858463
2216	20	-2.736329773030647
2216	21	-2.4058645192159602
2216	22	-2.687478854918389
2216	23	-1.9583328143289194
2216	24	-2.288494287435533
2216	25	-2.0997717606770436
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	72228.0
3	1.0
4	1149.0
5	10343.0
6	12011.0
7	9456.0
8	7781.0
9	6780.0
10	5891.0
11	5396.0
12	4957.0
13	4815.0
14	4571.0
15	4781.0
16	4748.0
17	5101.0
18	5639.0
19	6626.0
20	8483.0
21	10999.0
22	13956.0
23	17157.0
24	20556.0
25	23859.0
26	26922.0
27	30057.0
28	33980.0
29	41175.0
30	51730.0
31	66352.0
32	84531.0
33	108731.0
34	142647.0
35	186716.0
36	257075.0
37	400925.0
38	1358174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.99212756224256	19.75523107296143	14.243771487439169	27.008869877356844
2	13.593774948499753	20.663639909223452	40.22761538803833	25.514969754238464
3	16.52151834620893	24.86154005220039	30.418587971922907	28.19835362966778
4	11.41358813013803	17.71527936422114	37.831257556288826	33.039874949352
5	12.890972386848684	37.99005386941676	36.088580024177716	13.030393719556837
6	31.59368078576939	37.74059210379826	17.96773593579932	12.69799117463303
7	27.496928161865743	31.73177697898712	22.60925236991412	18.16204248923302
8	24.803260336792764	37.07081121734498	20.645558210219953	17.4803702356423
9	25.891654409995553	15.191310459378832	20.360030234395126	38.55700489623049
10	14.38857616409235	29.439044323212403	34.76890119770776	21.403478314987478
11	33.781554784724456	22.65622827129401	24.896724738584748	18.665492205396784
12	22.509338477472305	24.997799342584276	32.01133543091163	20.481526749031794
13	28.25501388036318	22.099193878766382	26.207676671958787	23.438115568911652
14	20.76936895607191	22.00792326597979	27.97249271366461	29.250215064283687
15	22.730338170605886	30.75890828095382	23.659638992808063	22.851114555632236
16	22.189380482319784	28.368587297577143	27.384273228149592	22.057758991953484
17	21.52370616155315	28.689001579516173	27.858953722729122	21.928338536201554
18	21.394923784123893	28.01521994416038	29.283799476025052	21.30605679569067
19	23.07755986965432	27.288894211435778	27.743088514718206	21.8904574041917
20	23.22798124296292	27.559762615043258	27.973880717887607	21.238375424106216
21	22.84430097366287	26.911252647814642	27.463948167290187	22.7804982112323
22	22.84589294560246	27.814452742846036	27.4985456165964	21.841108694955107
23	22.28024810399768	27.555975380681012	27.71139211183199	22.45238440348932
24	22.70995825668832	27.129880659543016	28.09526427069612	22.064896813072547
25	22.651997335228515	27.336026899009614	27.888646319644312	22.123329446117562
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	129.0
1	129.0
2	353.5
3	578.0
4	578.0
5	578.0
6	1489.5
7	2401.0
8	2401.0
9	2401.0
10	3669.0
11	4937.0
12	4937.0
13	4937.0
14	9210.0
15	13483.0
16	13483.0
17	13483.0
18	25198.0
19	36913.0
20	36913.0
21	36913.0
22	58836.0
23	80759.0
24	80759.0
25	80759.0
26	115002.5
27	149246.0
28	149246.0
29	149246.0
30	191028.5
31	232811.0
32	232811.0
33	232811.0
34	264758.0
35	296705.0
36	296705.0
37	296705.0
38	322428.5
39	348152.0
40	348152.0
41	348152.0
42	366542.0
43	384932.0
44	384932.0
45	384932.0
46	389992.5
47	395053.0
48	395053.0
49	395053.0
50	382139.0
51	369225.0
52	369225.0
53	369225.0
54	330020.5
55	290816.0
56	290816.0
57	290816.0
58	249309.0
59	207802.0
60	207802.0
61	207802.0
62	167816.0
63	127830.0
64	127830.0
65	127830.0
66	97792.5
67	67755.0
68	67755.0
69	67755.0
70	49044.5
71	30334.0
72	30334.0
73	30334.0
74	20737.0
75	11140.0
76	11140.0
77	11140.0
78	7482.0
79	3824.0
80	3824.0
81	3824.0
82	2466.5
83	1109.0
84	1109.0
85	1109.0
86	708.0
87	307.0
88	307.0
89	307.0
90	179.5
91	52.0
92	52.0
93	52.0
94	29.0
95	6.0
96	6.0
97	6.0
98	3.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08114389331672066
2	9.815793546377498E-5
3	0.0
4	0.02934922270366872
5	0.07352029366236745
6	0.07770836557548852
7	0.036809225798915615
8	0.06988845005020779
9	0.04907896773188749
10	0.11072215120313818
11	0.004777019525903715
12	0.012891408857575781
13	0.0059876340632902736
14	0.007721757589816965
15	0.0077871962134594816
16	0.018551849802653474
17	0.030527117929234016
18	0.001505088343777883
19	0.0038608787949084827
20	0.005431405762328882
21	1.6359655910629163E-4
22	6.871055482464249E-4
23	0.0
24	3.2719311821258325E-5
25	0.004155352601299807
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3056299.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.2671380945941
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.4789684160375	54.25801712473515
2	13.456241795749497	18.372382338093594
3	3.769030873870866	7.719028534479932
4	1.4157437503284513	3.865950964409238
5	0.6402733359368734	2.1854814121344486
6	0.3559684380098624	1.4580567908961741
7	0.20276073123594468	0.968932638761158
8	0.13737629832412393	0.75026293828937
9	0.0869780058996587	0.5343965585949979
>10	0.41888166864355764	4.85223439384661
>50	0.022022461974049203	1.0255526414443716
>100	0.012975720511317578	1.685038560493835
>500	0.0017724935988538044	0.852123207678097
>1k	9.5810464717685E-4	1.3024181272103217
>5k	4.79052323588425E-5	0.17012376893278325
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5202	0.1702058600941858	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3953	0.12933943962943414	No Hit
GTCCTACAGTGGACATTTCTAAATT	3515	0.11500838105172302	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3301	0.10800644832197374	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3238	0.10594513167723446	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3100	0.10142986664590081	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	3.2719311821258325E-5
6	0.0	0.0	0.0	0.0	3.2719311821258325E-5
7	0.0	0.0	0.0	0.0	3.2719311821258325E-5
8	0.0	0.0	0.0	0.0	3.2719311821258325E-5
9	0.0	0.0	0.0	0.0	3.2719311821258325E-5
10	3.2719311821258325E-5	0.0	0.0	0.0	3.2719311821258325E-5
11	3.2719311821258325E-5	0.0	0.0	0.0	3.2719311821258325E-5
12	3.2719311821258325E-5	0.0	0.0	0.0	3.2719311821258325E-5
13	3.2719311821258325E-5	0.0	0.0	0.0	3.2719311821258325E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1340	0.0	12.842475	1
AACACCG	120	9.780706E-9	11.894944	5
GTCCTAA	1010	0.0	11.202127	1
GGGCGAA	60	0.00591496	11.073968	11
TAGGACC	795	0.0	11.012176	4
TAGTACG	70	0.0014713712	10.875379	4
GTCCTAC	2005	0.0	10.432376	1
TCCAACG	230	0.0	10.314323	18
TGGCGAG	2115	0.0	10.139735	18
AGGACCT	1520	0.0	10.016796	5
TTAGGAC	1400	0.0	9.982909	3
GGCGAGG	990	0.0	9.872902	19
GACAGCG	145	1.6062404E-7	9.832799	7
GTATTAA	395	0.0	9.628039	1
GGACGTG	1895	0.0	9.588763	6
TCCTACA	2390	0.0	9.586779	2
AGGACGT	1930	0.0	9.565262	5
GGCGAGA	1225	0.0	9.52822	19
GTAGGAC	2010	0.0	9.460231	3
TAGGACA	765	0.0	9.45376	4
>>END_MODULE