FastQCFastQC Report
Thu 26 May 2016
SRR1512990_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512990_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638480
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT41580.15759073405900367No Hit
CTGTAGGACGTGGAATATGGCAAGA35790.13564628119220157No Hit
GTCCTAAAGTGTGTATTTCTCATTT32260.12226736605924624No Hit
CTTTAGGACGTGAAATATGGCGAGG28750.1089642521451745No Hit
GTCCTACAGTGTGCATTTCTCATTT27520.10430247718383312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGG500.001502446413.2971613
TAGGACC8650.012.7371644
GGTATCA10300.012.02329351
TCCAACG2250.011.8246318
TGTAGGA22900.011.405862
GTAGGAC21550.010.9303813
CTGTAGG22600.010.9171331
GGACGTG19700.010.8926386
AAAAGCG803.780666E-410.6850165
CCAACGA1605.493348E-1010.6821819
AGGACGT19850.010.6700635
GTATCAA21650.010.6041641
TAGGACG21100.010.4882814
GGCGAGG9150.010.48111919
GTCCTAA11300.010.4534711
CTAGGAC4200.010.4025413
CGGTTTC1659.258656E-1010.3661413
TTAGGAC12850.010.2740713
GACGTGA11000.010.2717687
GTCCTAC22450.010.09898951
Produced by FastQC (version 0.11.5)