##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512990_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2638480 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.43828567963373 31.0 30.0 33.0 16.0 34.0 2 28.63607948515812 31.0 30.0 34.0 16.0 34.0 3 28.755422061186742 31.0 30.0 34.0 16.0 34.0 4 32.18861579394196 35.0 33.0 37.0 20.0 37.0 5 32.305801446287255 35.0 33.0 37.0 22.0 37.0 6 32.32480177981262 35.0 33.0 37.0 22.0 37.0 7 32.13995937054668 35.0 33.0 37.0 19.0 37.0 8 32.12018434856432 35.0 33.0 37.0 19.0 37.0 9 33.4079443467451 38.0 33.0 39.0 18.0 39.0 10 33.27483210030017 38.0 33.0 39.0 17.0 39.0 11 33.324988250811074 38.0 33.0 39.0 17.0 39.0 12 33.21468724417089 38.0 33.0 39.0 17.0 39.0 13 33.21587997635002 38.0 33.0 39.0 17.0 39.0 14 34.14730071859555 39.0 33.0 41.0 17.0 41.0 15 34.07357721112155 39.0 33.0 41.0 17.0 41.0 16 33.97116332130621 39.0 33.0 41.0 16.0 41.0 17 33.88804235772111 39.0 33.0 41.0 11.0 41.0 18 33.60427443073284 39.0 33.0 41.0 10.0 41.0 19 33.822191943846455 39.0 33.0 41.0 10.0 41.0 20 33.88899972711561 39.0 33.0 41.0 10.0 41.0 21 33.82159311421728 39.0 33.0 41.0 10.0 41.0 22 33.72208506412783 39.0 33.0 41.0 2.0 41.0 23 33.442833752766745 39.0 33.0 41.0 2.0 41.0 24 33.43603855249992 39.0 33.0 41.0 2.0 41.0 25 33.33478290530912 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5782516060228211 1101 2 0.8419498350699435 1101 3 0.8594342085583584 1101 4 0.8283021795974719 1101 5 0.6317760139995201 1101 6 0.8235148348473302 1101 7 0.955981536384023 1101 8 0.7217144447899244 1101 9 0.6839597905479309 1101 10 0.7578784897952247 1101 11 0.9584777634359014 1101 12 1.1084854326611122 1101 13 0.9298537081611826 1101 14 0.8932642102555022 1101 15 0.5898721460539917 1101 16 1.1603410390518363 1101 17 1.1988935805549588 1101 18 1.5124302412804411 1101 19 1.0418845162314412 1101 20 1.0269053887530717 1101 21 1.1303471475859084 1101 22 1.193313332379347 1101 23 1.4069753065192572 1101 24 0.9253131463815265 1101 25 1.1933065111301673 1102 1 0.5291082158552811 1102 2 0.6028905906219286 1102 3 0.603936584922554 1102 4 0.8082812652815647 1102 5 0.6930672497876884 1102 6 0.6398801032170809 1102 7 0.8058638080085743 1102 8 0.8448226629815991 1102 9 0.9287828145731325 1102 10 0.9404128826051519 1102 11 0.8960089812825061 1102 12 0.8742844861071148 1102 13 0.8353821360317397 1102 14 0.9005947252508406 1102 15 1.0231419801207977 1102 16 1.0550168172592507 1102 17 0.9169253238855717 1102 18 1.2733205755786088 1102 19 1.1869869055160862 1102 20 1.1690901731943342 1102 21 0.9617584332280913 1102 22 1.236644097380065 1102 23 1.5191184776694726 1102 24 1.3881671259052482 1102 25 1.2055310390743088 1103 1 0.30540308274885675 1103 2 0.44039645588549803 1103 3 0.44441382486678194 1103 4 0.5067251522816179 1103 5 0.0888161668139773 1103 6 0.5197583720479528 1103 7 0.35112613409971516 1103 8 0.3181862211563171 1103 9 0.7115985003263532 1103 10 0.6595282220889587 1103 11 0.7456533427505718 1103 12 0.8150136639468926 1103 13 0.7460688119944336 1103 14 0.9967711655247058 1103 15 0.9562330704900788 1103 16 1.0413696287922178 1103 17 0.9464341229000084 1103 18 1.1480779827244234 1103 19 1.1633298560968086 1103 20 1.0788027096926882 1103 21 0.8986091285065356 1103 22 0.9738516749294561 1103 23 1.354901693561544 1103 24 1.1259425170639972 1103 25 1.1330735801900857 1104 1 0.5882642203400721 1104 2 0.6884406020905125 1104 3 0.5861327614158505 1104 4 0.8765081433663511 1104 5 0.5460862561243331 1104 6 0.5888972939383947 1104 7 0.9027824053703242 1104 8 0.7871512155247089 1104 9 0.8503724188810935 1104 10 0.9305895168108904 1104 11 1.007384522607559 1104 12 0.8417013827644766 1104 13 1.0246362863874694 1104 14 1.1319025647879002 1104 15 1.112945834690592 1104 16 0.9821462880094032 1104 17 0.9542546653862445 1104 18 1.238454278562756 1104 19 1.0663860755012138 1104 20 1.0682698374022834 1104 21 1.270006911417859 1104 22 1.03652788089191 1104 23 1.326926951636466 1104 24 1.1996044670304613 1104 25 0.7824126465939187 1105 1 0.7294181741911849 1105 2 0.8375130510271447 1105 3 0.7989440641351848 1105 4 0.8354946637639671 1105 5 0.607066136407127 1105 6 0.4129676099208055 1105 7 0.7585076554717389 1105 8 0.8388915613729395 1105 9 0.6585982821372269 1105 10 0.8836155161305683 1105 11 0.8848897413840646 1105 12 0.8899802724877333 1105 13 0.9407019203404445 1105 14 0.9370219445092189 1105 15 1.2033764107619902 1105 16 1.2159186275026528 1105 17 1.0115846321789874 1105 18 1.100217237067163 1105 19 1.0736587811051947 1105 20 1.1326615533867894 1105 21 1.159498776007453 1105 22 0.8969029155036878 1105 23 1.1706393256000283 1105 24 1.193180654930032 1105 25 1.2243060728401503 1106 1 0.632324624468378 1106 2 0.8326556469258932 1106 3 0.7794330908340434 1106 4 0.7477297805574779 1106 5 0.7622051269921357 1106 6 0.7812679605944197 1106 7 0.5766057233172077 1106 8 0.866026625190635 1106 9 0.9434503566442487 1106 10 1.2168067784661574 1106 11 1.0325906473819657 1106 12 1.0988698987769894 1106 13 1.031917461914098 1106 14 1.3535818923436622 1106 15 1.3265223262870407 1106 16 1.2858645842249743 1106 17 1.2088164886252386 1106 18 1.3987483642487462 1106 19 1.0792258226648812 1106 20 1.1320164936546036 1106 21 1.024658551789173 1106 22 1.079431196408187 1106 23 1.26586105957049 1106 24 1.2231478660034867 1106 25 1.3526055116983429 1107 1 0.5287189594001802 1107 2 0.7034320458504268 1107 3 0.7407239394435869 1107 4 0.8590970019873652 1107 5 0.6742951778788537 1107 6 0.5113934382122167 1107 7 0.7224873444593598 1107 8 0.6934658047433615 1107 9 0.8547862136012156 1107 10 0.7154012950094355 1107 11 0.7774225778619837 1107 12 1.0890380899411483 1107 13 0.9692062695315187 1107 14 1.2004798670666617 1107 15 1.2010456947919792 1107 16 1.1615409423165204 1107 17 1.276786326128338 1107 18 1.5682611760112763 1107 19 1.404448119188686 1107 20 1.2649748998922519 1107 21 1.286401653742125 1107 22 1.3263415985638503 1107 23 1.2849536071218566 1107 24 1.253120663734201 1107 25 1.3862618267738753 1108 1 0.6022133262191929 1108 2 0.7468248027897388 1108 3 0.5674741203673008 1108 4 0.7903157548524007 1108 5 0.7039494363972238 1108 6 0.8245936845155555 1108 7 0.9301815928082888 1108 8 0.8509916786227905 1108 9 1.05791006555652 1108 10 1.0260208181250832 1108 11 0.8419471597542696 1108 12 0.9068805819483146 1108 13 0.9834441369139455 1108 14 1.1605170206375703 1108 15 1.202471302146229 1108 16 1.0836332072343566 1108 17 1.2227478422073048 1108 18 1.3393378948190815 1108 19 1.3106129200490528 1108 20 1.2528287902560535 1108 21 1.279166655780564 1108 22 1.139962212098439 1108 23 1.3504024767468934 1108 24 1.2523306464318438 1108 25 1.4297791008435539 1109 1 0.0773089964728193 1109 2 0.04178653633112006 1109 3 0.33374299197580015 1109 4 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-4.337907183971261 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 152556.0 3 1.0 4 1233.0 5 7218.0 6 8797.0 7 7862.0 8 6869.0 9 6552.0 10 6184.0 11 5856.0 12 5690.0 13 5487.0 14 5598.0 15 5600.0 16 5869.0 17 6238.0 18 7057.0 19 8723.0 20 10684.0 21 13671.0 22 17335.0 23 20970.0 24 23985.0 25 27460.0 26 30483.0 27 33316.0 28 38241.0 29 45118.0 30 54207.0 31 67000.0 32 83819.0 33 106067.0 34 134861.0 35 174358.0 36 232864.0 37 354556.0 38 916095.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1643924922958 18.608862547668657 12.891590461475966 25.335154498559575 2 14.816865115400818 19.689554719791246 41.49109739122983 24.0024827735781 3 18.816566476903272 24.075344218662025 29.435733527456925 27.672355776977774 4 12.214302234321266 16.47126624176774 38.30926373180562 33.005167792105375 5 13.257792344687738 37.657033699208135 35.9041258362618 13.181048119842325 6 32.364409407508326 36.87214222408223 17.9636827903796 12.79976557802984 7 28.271303221805365 31.442918916009255 22.019504979732698 18.266272882452682 8 24.94717216604355 37.06372018648775 20.431918247457933 17.557189400010767 9 26.232017013468994 15.224856041335755 20.032222479159635 38.51090446603561 10 14.752138910023053 28.983259088922324 34.6311794682834 21.63342253277122 11 34.45954424130123 22.305511402686424 24.6536774464533 18.581266909559044 12 23.169752555230975 24.483021105248753 31.78069707898869 20.566529260531585 13 28.95541998634536 21.823315317951835 25.685622557981997 23.535642137720814 14 20.856569853624137 21.895114359695647 27.45648720680282 29.791828579877393 15 22.922576139793776 30.906497065261814 23.113612772271747 23.057314022672664 16 22.457014789126436 28.074547629842705 27.37209066054459 22.09634692048627 17 21.79746179942552 28.552766207972923 27.6512754880579 21.998496504543656 18 21.803520107311833 27.62615816962917 29.23598739935722 21.33433432370177 19 23.498789144303366 27.04677830498399 27.238149612329043 22.216282938383593 20 23.634586026643536 27.444374480403265 27.67573600296907 21.245303489984128 21 23.20038123154926 26.78583817296979 26.86814263731406 23.14563795816689 22 23.127915876431853 27.90693001972381 27.04682347402072 21.918330629823622 23 22.69406029175386 27.55625815060807 27.24706578109362 22.502615776544445 24 23.16094174036294 26.81489192245392 27.930902850566362 22.093263486616777 25 22.8693378905939 27.231729572885715 27.618598839505932 22.280333697014456 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 162.0 1 162.0 2 324.5 3 487.0 4 487.0 5 487.0 6 915.0 7 1343.0 8 1343.0 9 1343.0 10 2206.0 11 3069.0 12 3069.0 13 3069.0 14 6026.0 15 8983.0 16 8983.0 17 8983.0 18 17895.5 19 26808.0 20 26808.0 21 26808.0 22 44143.5 23 61479.0 24 61479.0 25 61479.0 26 89446.5 27 117414.0 28 117414.0 29 117414.0 30 154974.0 31 192534.0 32 192534.0 33 192534.0 34 221123.5 35 249713.0 36 249713.0 37 249713.0 38 273569.5 39 297426.0 40 297426.0 41 297426.0 42 314716.5 43 332007.0 44 332007.0 45 332007.0 46 337913.0 47 343819.0 48 343819.0 49 343819.0 50 333460.0 51 323101.0 52 323101.0 53 323101.0 54 291083.5 55 259066.0 56 259066.0 57 259066.0 58 223429.0 59 187792.0 60 187792.0 61 187792.0 62 153282.0 63 118772.0 64 118772.0 65 118772.0 66 92139.5 67 65507.0 68 65507.0 69 65507.0 70 48008.0 71 30509.0 72 30509.0 73 30509.0 74 21355.5 75 12202.0 76 12202.0 77 12202.0 78 8317.5 79 4433.0 80 4433.0 81 4433.0 82 2903.0 83 1373.0 84 1373.0 85 1373.0 86 864.5 87 356.0 88 356.0 89 356.0 90 224.5 91 93.0 92 93.0 93 93.0 94 54.5 95 16.0 96 16.0 97 16.0 98 16.0 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35603832509626754 2 0.0419559746520724 3 0.03733210030017283 4 0.037521603347381825 5 0.0435098996391862 6 0.019026105939783513 7 0.018457596798156516 8 0.025241805888238683 9 0.0216033473818259 10 0.021641247991267702 11 0.020807434583548102 12 0.0211485400685243 13 0.020807434583548102 14 0.02842545708134987 15 0.029031866832418665 16 0.022588763227312694 17 0.02376368212000849 18 0.09187107728692277 19 0.025620811982656682 20 0.02338467602559049 21 0.027023134532003272 22 0.020883235802431704 23 0.024256390042751888 24 0.019443012643643308 25 0.02418058882386829 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2638480.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.39892777866524 #Duplication Level Percentage of deduplicated Percentage of total 1 81.29549273019336 57.231155214438864 2 12.352383367807988 17.391890892094008 3 3.3918544874225596 7.163487572874072 4 1.2560347438081323 3.5369399686737206 5 0.5878048966626389 2.0690417234049456 6 0.3241354982293513 1.3691274918209866 7 0.18765781162066617 0.9247636109169952 8 0.11875266581717865 0.6688048275510023 9 0.08435669277894493 0.5344758650332797 >10 0.36796100321260683 4.385251026753767 >50 0.01892296946851513 0.9153475789435973 >100 0.01184097481143199 1.6218879804190636 >500 0.0018321803397974964 0.86650377679079 >1k 9.699778268757713E-4 1.3213224702849444 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4158 0.15759073405900367 No Hit CTGTAGGACGTGGAATATGGCAAGA 3579 0.13564628119220157 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3226 0.12226736605924624 No Hit CTTTAGGACGTGAAATATGGCGAGG 2875 0.1089642521451745 No Hit GTCCTACAGTGTGCATTTCTCATTT 2752 0.10430247718383312 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.790060944179982E-5 0.0 5 0.0 0.0 0.0 3.790060944179982E-5 0.0 6 0.0 0.0 0.0 3.790060944179982E-5 0.0 7 0.0 0.0 0.0 7.580121888359964E-5 0.0 8 0.0 0.0 0.0 7.580121888359964E-5 0.0 9 0.0 0.0 0.0 1.1370182832539947E-4 0.0 10 0.0 0.0 0.0 1.1370182832539947E-4 0.0 11 0.0 0.0 0.0 1.1370182832539947E-4 0.0 12 0.0 0.0 0.0 1.1370182832539947E-4 0.0 13 0.0 0.0 0.0 1.1370182832539947E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACGGG 50 0.0015024464 13.297161 3 TAGGACC 865 0.0 12.737164 4 GGTATCA 1030 0.0 12.0232935 1 TCCAACG 225 0.0 11.82463 18 TGTAGGA 2290 0.0 11.40586 2 GTAGGAC 2155 0.0 10.930381 3 CTGTAGG 2260 0.0 10.917133 1 GGACGTG 1970 0.0 10.892638 6 AAAAGCG 80 3.780666E-4 10.685016 5 CCAACGA 160 5.493348E-10 10.68218 19 AGGACGT 1985 0.0 10.670063 5 GTATCAA 2165 0.0 10.604164 1 TAGGACG 2110 0.0 10.488281 4 GGCGAGG 915 0.0 10.481119 19 GTCCTAA 1130 0.0 10.453471 1 CTAGGAC 420 0.0 10.402541 3 CGGTTTC 165 9.258656E-10 10.36614 13 TTAGGAC 1285 0.0 10.274071 3 GACGTGA 1100 0.0 10.271768 7 GTCCTAC 2245 0.0 10.0989895 1 >>END_MODULE