FastQCFastQC Report
Thu 26 May 2016
SRR1512990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512990_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638480
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT39560.14993481095176012No Hit
CTGTAGGACGTGGAATATGGCAAGA35480.13447136229950576No Hit
GTATCAACGCAGAGTACTTTTTTTT32410.12283587520087323No Hit
CTTTAGGACGTGAAATATGGCGAGG31770.12041023619659805No Hit
GTCCTAAAGTGTGTATTTCTCATTT30450.11540735575028047No Hit
GTCCTACAGTGTGCATTTCTCATTT26890.10191473878899972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8400.013.9353184
GTGCGCC802.8949462E-511.86420711
GGTATCA9350.011.7966281
GTAGGAC23450.011.3944013
TGTAGGA24050.011.2678562
GGCGAGG8400.010.95777419
GTATCAA20150.010.8061741
AGGACGT20550.010.6977215
GGACGTG20800.010.6118696
TGGCGAG20250.010.59042418
AGGACCT16250.010.424565
CTGTAGG24850.010.4076811
TTAGGAC13000.010.1671553
TAGGACG22000.010.1656754
GGACCTG16050.010.07722956
TCCAACG1804.0199666E-1010.01638518
GACGTGG11200.010.0136887
TAGGACA7650.09.9522124
GTCCTAC19650.09.9197971
GTAGAAC7200.09.9050523
Produced by FastQC (version 0.11.5)