##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512990_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2638480
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.731073951669142	31.0	31.0	34.0	27.0	34.0
2	30.84872009641915	31.0	31.0	34.0	27.0	34.0
3	30.959751826809374	31.0	31.0	34.0	27.0	34.0
4	34.4746880779843	37.0	35.0	37.0	31.0	37.0
5	34.181165671143994	37.0	35.0	37.0	30.0	37.0
6	34.075056850914166	37.0	35.0	37.0	30.0	37.0
7	33.95856515872775	37.0	35.0	37.0	30.0	37.0
8	33.845234756374886	37.0	35.0	37.0	29.0	37.0
9	35.334204163002944	39.0	35.0	39.0	29.0	39.0
10	35.22206232376217	39.0	35.0	39.0	29.0	39.0
11	35.27847093781268	39.0	35.0	39.0	29.0	39.0
12	35.132542600285014	39.0	35.0	39.0	28.0	39.0
13	35.13820760437828	39.0	35.0	39.0	28.0	39.0
14	36.22614687244171	40.0	36.0	41.0	28.0	41.0
15	36.24493230951154	40.0	36.0	41.0	28.0	41.0
16	36.218784300051546	40.0	36.0	41.0	28.0	41.0
17	36.190049194991055	40.0	36.0	41.0	28.0	41.0
18	36.11873351323489	40.0	36.0	41.0	27.0	41.0
19	36.097814271853494	40.0	36.0	41.0	27.0	41.0
20	36.07211652163367	40.0	36.0	41.0	27.0	41.0
21	36.021362299505775	40.0	36.0	41.0	27.0	41.0
22	35.988214805494074	40.0	36.0	41.0	27.0	41.0
23	35.8868143779752	40.0	36.0	41.0	27.0	41.0
24	35.87716222976866	40.0	36.0	41.0	27.0	41.0
25	35.77953708195628	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.42261470757997444
1101	2	0.5248250623994366
1101	3	0.48207612014904555
1101	4	0.25097967104264995
1101	5	0.44110309495484756
1101	6	0.4962213953459482
1101	7	0.5142021652422386
1101	8	0.5460401760979536
1101	9	0.3451700555799917
1101	10	0.7262501282260132
1101	11	0.729396505211227
1101	12	0.7560890190458451
1101	13	0.8068612194216414
1101	14	0.919918892947102
1101	15	0.9183337450371312
1101	16	0.9970013309610977
1101	17	0.875872460758444
1101	18	0.32222738116096394
1101	19	0.7317150210661509
1101	20	0.6562686125521893
1101	21	0.910152331115782
1101	22	0.821538075479836
1101	23	0.7791245994048595
1101	24	0.7211753842908664
1101	25	0.844570278079452
1102	1	0.6619334113965998
1102	2	0.5547127858220513
1102	3	0.5064001733455719
1102	4	0.4536358206761193
1102	5	0.6640102058747743
1102	6	0.5584265163595816
1102	7	0.6156239920777935
1102	8	0.6620915196405406
1102	9	0.773832122357696
1102	10	0.8413203582047046
1102	11	0.7689719372452188
1102	12	0.8044403753513123
1102	13	0.9154701758119828
1102	14	1.0422902255230682
1102	15	1.0144429574729017
1102	16	0.9333313674797452
1102	17	1.0147079083704966
1102	18	1.0011148992220171
1102	19	1.0586532587067765
1102	20	1.024706693414224
1102	21	1.0084758769484452
1102	22	1.0468869406602295
1102	23	1.1265601271656962
1102	24	1.0913472209743276
1102	25	1.0727479856876556
1103	1	0.5498681626377468
1103	2	0.439074124701726
1103	3	0.5191986161729822
1103	4	0.5604509042216677
1103	5	0.5745157889030779
1103	6	0.6420737167257116
1103	7	0.5496748296694491
1103	8	0.5051105201763946
1103	9	0.6505249460742064
1103	10	0.6520036174264874
1103	11	0.5586735741700863
1103	12	0.6652377044231841
1103	13	0.6692074699129762
1103	14	0.8155211314234734
1103	15	0.5426084900139045
1103	16	0.7176457162560084
1103	17	0.7854958728524863
1103	18	0.7994468950401057
1103	19	0.8972623969758189
1103	20	0.8706441459771952
1103	21	0.4499576727172041
1103	22	0.7160655635119255
1103	23	0.8178585493396753
1103	24	0.8761962925358944
1103	25	0.8424869597145843
1104	1	0.5940555429280927
1104	2	0.6174001351069585
1104	3	0.5137608322973364
1104	4	0.586327791158979
1104	5	0.5589768817028045
1104	6	0.6024269386375778
1104	7	0.5827487155768694
1104	8	0.5229051678636054
1104	9	0.7108100569815861
1104	10	0.8639389049876343
1104	11	0.8104259736579138
1104	12	0.8054337753442411
1104	13	0.7585063366296723
1104	14	0.9273473242165906
1104	15	0.8732901205233077
1104	16	0.9647647424331183
1104	17	0.7721897809168254
1104	18	0.915678448121966
1104	19	0.8254464061882629
1104	20	0.9479396591901263
1104	21	0.883679784775687
1104	22	1.002597327731145
1104	23	0.8769118379205736
1104	24	0.9524203473239083
1104	25	1.04895725730087
1105	1	0.4349924589413803
1105	2	0.4650575276366986
1105	3	0.5023626702244464
1105	4	0.48526240558657463
1105	5	0.4630819330953031
1105	6	0.5429849016406791
1105	7	0.5765672137587927
1105	8	0.6601596581499223
1105	9	0.7918295712293855
1105	10	0.8016101956911399
1105	11	0.7252872403008084
1105	12	0.6732023644022647
1105	13	0.765394386494691
1105	14	0.9505166647427927
1105	15	0.7986582194139729
1105	16	0.8999677094559786
1105	17	0.6391662809962355
1105	18	0.942448415859225
1105	19	1.0126166475673983
1105	20	1.0336530093004725
1105	21	0.8993158428624355
1105	22	0.9968065519394926
1105	23	0.95058112509507
1105	24	0.9586903416813257
1105	25	0.9977601186450329
1106	1	0.31696181686546865
1106	2	0.39986658896246396
1106	3	0.3527631790748522
1106	4	0.3269631908186028
1106	5	0.3328794383197504
1106	6	0.3131862770689793
1106	7	0.3906470567764089
1106	8	0.4586387919826791
1106	9	0.4724555689414558
1106	10	0.520661817138496
1106	11	0.4111834980050091
1106	12	0.6621675764363317
1106	13	0.5858638367594082
1106	14	0.7400056407729707
1106	15	0.6522579198616327
1106	16	0.7426864493933039
1106	17	0.6704781990308746
1106	18	0.8353652990268543
1106	19	0.749690201376815
1106	20	0.8588747730760318
1106	21	0.7755721142151728
1106	22	0.7657977849336106
1106	23	0.9063883792380025
1106	24	0.7757343023337313
1106	25	0.7845717683785409
1107	1	0.5308060426133991
1107	2	0.5526385311071778
1107	3	0.470954310359172
1107	4	0.6392517518599874
1107	5	0.5690493167215962
1107	6	0.5780855523984201
1107	7	0.5836020175247896
1107	8	0.6570799053491356
1107	9	0.5988971346927698
1107	10	0.6470458484792942
1107	11	0.6684116281892685
1107	12	0.6112660615388634
1107	13	0.7219383066702321
1107	14	0.8541331988350578
1107	15	0.7756906003273514
1107	16	0.7768799714789552
1107	17	0.7259982476359426
1107	18	0.8768334719772639
1107	19	0.9698277053302533
1107	20	0.9216882123252859
1107	21	0.7927410398471793
1107	22	0.9480628522094179
1107	23	0.9008614593804793
1107	24	1.0438604361725794
1107	25	0.9783670602884129
1108	1	0.5101241699017685
1108	2	0.3955164438489547
1108	3	0.4078133966310098
1108	4	0.4405194857977648
1108	5	0.5306863366072747
1108	6	0.528753360851411
1108	7	0.4847233869401322
1108	8	0.5489782291469965
1108	9	0.5477147421302462
1108	10	0.6740423327397451
1108	11	0.5499613755462889
1108	12	0.5817255915208364
1108	13	0.6538996107774722
1108	14	0.7592339146622535
1108	15	0.8150256593445704
1108	16	0.6817498592054676
1108	17	0.704335192941393
1108	18	0.7991120249256554
1108	19	0.6553445749630953
1108	20	0.8804008677522432
1108	21	0.9192426416641553
1108	22	0.9973508287963
1108	23	0.8248917050140889
1108	24	0.8673370844965405
1108	25	0.9315873073590097
1109	1	0.08792321686274462
1109	2	0.11489481637530119
1109	3	0.19218922112673553
1109	4	0.3715725042201399
1109	5	0.37955542828939315
1109	6	-0.006600522981393908
1109	7	0.22994102424053864
1109	8	0.4445682797691859
1109	9	0.481427503673018
1109	10	0.3874005516745527
1109	11	0.3485422654151833
1109	12	0.4481540805731754
1109	13	0.32993468455865127
1109	14	0.5072147934256108
1109	15	0.5167455718014011
1109	16	0.30906497748765105
1109	17	0.435912734392474
1109	18	0.43308504372917156
1109	19	0.6900262124185872
1109	20	0.6319154756397367
1109	21	0.6693864715036781
1109	22	0.7007712354495013
1109	23	0.6606457495814624
1109	24	0.20028665254702815
1109	25	0.34146883625624014
1110	1	0.20584134452849057
1110	2	0.17689416245325873
1110	3	0.2309747734755767
1110	4	0.3098164728698407
1110	5	0.1788612572951962
1110	6	0.27723323733199123
1110	7	0.24319163934247712
1110	8	0.33338096368476045
1110	9	0.5555682875709849
1110	10	0.5061603530318877
1110	11	0.33017776273024424
1110	12	0.438829136880841
1110	13	0.07091123776199737
1110	14	0.3729819086503312
1110	15	0.5213053095436706
1110	16	0.6083407212526666
1110	17	0.4484192540040226
1110	18	0.27761889006384877
1110	19	0.4767368293163088
1110	20	0.3334191507836408
1110	21	0.4316628815319987
1110	22	0.4425435528703474
1110	23	0.47696320531241554
1110	24	0.44400277271268607
1110	25	0.49798997537505585
1111	1	-0.3499342883751808
1111	2	-0.08687174843746703
1111	3	-0.07124348752852327
1111	4	0.023633980598170012
1111	5	0.23999332258595985
1111	6	0.010867613789415032
1111	7	0.2519770952361924
1111	8	0.3229535663349239
1111	9	-0.1087122259300557
1111	10	0.03317458319116895
1111	11	0.16396015684590282
1111	12	0.1687026199573225
1111	13	0.22000605974115217
1111	14	0.3404948761480142
1111	15	0.553388769471546
1111	16	0.07378925550618476
1111	17	0.4764742088341194
1111	18	0.4487271686415468
1111	19	0.39987718816341555
1111	20	0.30514455395880447
1111	21	0.2061623116046789
1111	22	0.03516183545801965
1111	23	0.18989873064747798
1111	24	0.33586077158828687
1111	25	0.31601988519645374
1112	1	0.029956385890475445
1112	2	-0.1043985832642349
1112	3	0.0817685231331744
1112	4	-0.04534831852888743
1112	5	0.14284371030045406
1112	6	0.1502929132884745
1112	7	0.07937146245075155
1112	8	0.3040193026312892
1112	9	-0.006135604012179385
1112	10	0.29426561934776174
1112	11	0.13033400607245937
1112	12	0.3247662795342521
1112	13	0.20853782813095734
1112	14	0.2736242846622048
1112	15	0.12651412386822614
1112	16	-0.004822927071764127
1112	17	0.07128751175705617
1112	18	0.00945247644748548
1112	19	0.0669594244471341
1112	20	0.456687962812488
1112	21	0.48267050652015087
1112	22	0.2908129953509686
1112	23	0.4444684163224508
1112	24	0.3769800420999516
1112	25	0.5200652443605662
1113	1	-0.3535217999664546
1113	2	-0.7640153710606796
1113	3	-0.33663466649782947
1113	4	-0.37367471475624825
1113	5	-0.2335154053443418
1113	6	-0.3178804173131482
1113	7	-0.23221781774363137
1113	8	-0.09652847713536516
1113	9	-0.2415133168519361
1113	10	-0.49204905209221295
1113	11	-0.26734637665450833
1113	12	-0.28012779481139205
1113	13	-0.15878597656615057
1113	14	-0.10550967337010064
1113	15	-0.08207530728908097
1113	16	-0.3613088515759273
1113	17	-0.2260038718541182
1113	18	-0.09156373768983173
1113	19	-0.20411113634419564
1113	20	0.03785434911943497
1113	21	-0.340110248582036
1113	22	-0.5025470620149406
1113	23	-0.07259578605413708
1113	24	-0.40065471028490407
1113	25	-0.3885231084901193
1114	1	-0.6052414646224733
1114	2	-0.4555323562653726
1114	3	-0.4072703141897378
1114	4	-0.2454785904715706
1114	5	-0.2987272879083065
1114	6	-0.726162468571161
1114	7	-0.5205104786011958
1114	8	-0.8530398082627002
1114	9	-0.4720511715093352
1114	10	-0.6641491339497492
1114	11	-0.48021395028644065
1114	12	-0.6056603610780229
1114	13	-0.549736475728352
1114	14	-1.5708737612586532
1114	15	-0.8988685499633959
1114	16	-0.8663377455052483
1114	17	-0.9892787640937826
1114	18	-1.08942189254779
1114	19	-1.177615970439689
1114	20	-0.37234028991507984
1114	21	-0.45145056496657787
1114	22	-0.7489708346441475
1114	23	-0.5718969964767666
1114	24	-1.2536481129236634
1114	25	-1.1706813767109239
1115	1	-1.7800737261055524
1115	2	-1.4191324955112954
1115	3	-1.1223483607902303
1115	4	-2.6544008004460373
1115	5	-1.5625202666843663
1115	6	-1.4941557428299106
1115	7	-1.286825676202774
1115	8	-1.232801246231027
1115	9	-1.8031977784173918
1115	10	-1.582166518088961
1115	11	-1.43978758802551
1115	12	-2.029000951303175
1115	13	-1.496784368711829
1115	14	-2.512744319189345
1115	15	-2.366054943376845
1115	16	-1.7707694984746638
1115	17	-1.719310731911797
1115	18	-1.6205588054531077
1115	19	-1.5502731927417415
1115	20	-1.6465568900254794
1115	21	-1.667964008875643
1115	22	-1.9799914326877186
1115	23	-2.2822788519378676
1115	24	-1.7376404957556346
1115	25	-1.556250918470404
1116	1	-1.2321421918750488
1116	2	-1.3932465223302266
1116	3	-1.1628268429181645
1116	4	-1.3737172273419844
1116	5	-1.2565557187655045
1116	6	-2.006806922818818
1116	7	-1.2697022130394444
1116	8	-1.5334107879356864
1116	9	-1.730733144836826
1116	10	-1.7157835989109174
1116	11	-1.5510607242500498
1116	12	-2.187579762770696
1116	13	-1.908326740103675
1116	14	-2.298128310772789
1116	15	-2.26681899086676
1116	16	-2.5936374037753467
1116	17	-2.4141810930361913
1116	18	-3.0838151008214254
1116	19	-2.4044084659218683
1116	20	-2.245664814003085
1116	21	-2.2297659598319584
1116	22	-3.728819868724578
1116	23	-3.6906421776260743
1116	24	-2.7551778626965415
1116	25	-3.3530626569719857
1201	1	0.6720093083544931
1201	2	0.6812604598790379
1201	3	0.6154343793271018
1201	4	0.6384398682526538
1201	5	0.6578197113272637
1201	6	0.7113303660184513
1201	7	0.7399810132393156
1201	8	0.7567828468689513
1201	9	0.8601274930368277
1201	10	0.7841858465159675
1201	11	0.8026865676107846
1201	12	0.7910055771668709
1201	13	0.8762218376443727
1201	14	0.8930377713458668
1201	15	0.9515199291570298
1201	16	1.0309857836733727
1201	17	0.8846437918549839
1201	18	0.9235239409132205
1201	19	0.8227403922613448
1201	20	1.0096159259815565
1201	21	0.9617256027927041
1201	22	1.0654868900557304
1201	23	0.9772741940361271
1201	24	0.9947749643771147
1201	25	0.9162883370737518
1202	1	0.5751555687301071
1202	2	0.6768150514547706
1202	3	0.5739644616007844
1202	4	0.5934907127911515
1202	5	0.6723101446738156
1202	6	0.7488878299500286
1202	7	0.5707156921446455
1202	8	0.4476144348731168
1202	9	0.755416998464014
1202	10	0.685339462481771
1202	11	0.7274592542120217
1202	12	0.6905737810166457
1202	13	0.7813953491266972
1202	14	0.7693276253119308
1202	15	0.9446784956050749
1202	16	0.8187242285576346
1202	17	0.849897403469086
1202	18	0.9181597304348301
1202	19	1.0765029076554313
1202	20	1.074972915042565
1202	21	1.1396282265609372
1202	22	1.0279686892038669
1202	23	0.9417974433842176
1202	24	0.7977433451809262
1202	25	0.9232500317717438
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2211	7	0.10424179305034897
2211	8	0.12606327724115118
2211	9	-0.2224751290814524
2211	10	-0.3131906139194598
2211	11	-0.10083049840770997
2211	12	-0.08240380276035353
2211	13	-0.12304714195857969
2211	14	-1.129278414297353
2211	15	-0.37176272515651476
2211	16	-0.16503288072091493
2211	17	-0.015550547580154728
2211	18	-0.15961112114131737
2211	19	-0.03236670733546276
2211	20	-0.19210066767914924
2211	21	-0.32591502673533057
2211	22	-0.15009877075685552
2211	23	-0.17997316227229732
2211	24	-0.26331045398922015
2211	25	-0.25021678437855144
2212	1	-0.056522032019660884
2212	2	0.023951186295139593
2212	3	0.010784955849075573
2212	4	0.1307342961034763
2212	5	8.868867656701696E-4
2212	6	1.223379575776562E-5
2212	7	0.013985908778970213
2212	8	-0.24930672961284728
2212	9	-0.2954717577422272
2212	10	-0.24512735475794756
2212	11	-0.12050886606370881
2212	12	0.025227687310618307
2212	13	-0.04588692150837659
2212	14	-0.13014284042861846
2212	15	-0.014250788079934296
2212	16	-0.035848387865272
2212	17	-0.20079192093427167
2212	18	-0.04110295917831763
2212	19	-0.05737211345007864
2212	20	-0.3613018946407678
2212	21	-0.12377775052031126
2212	22	-0.054815696876531206
2212	23	-0.5252987561552516
2212	24	-0.17181944647278868
2212	25	-0.37006520995672787
2213	1	-0.31158595577863935
2213	2	-0.34232004278187844
2213	3	-0.2363661348507584
2213	4	-0.2525765160610689
2213	5	-0.1518146517232637
2213	6	-0.017561735443365478
2213	7	-0.4624375441057822
2213	8	-0.25601165629475986
2213	9	-0.42460209186435804
2213	10	-0.2741435989393608
2213	11	-0.1407489780833302
2213	12	-0.3642304021670668
2213	13	-0.28141820218743874
2213	14	-0.16920478737067413
2213	15	-0.32150535000795344
2213	16	-0.5877108922340781
2213	17	-0.499530253150823
2213	18	-0.5885152880155786
2213	19	-0.8785342948052275
2213	20	-0.4145236832593753
2213	21	-1.0942838868895137
2213	22	-0.5597144406628018
2213	23	-0.6391366972380936
2213	24	-0.7082411852257522
2213	25	-0.8240416617929682
2214	1	-0.6539832600032476
2214	2	-0.606694441583759
2214	3	-0.5020039074892146
2214	4	-1.0702016284753384
2214	5	-0.8216888320170881
2214	6	-2.3887167461400303
2214	7	-0.871804857949563
2214	8	-1.2857003395038262
2214	9	-0.7102071665483649
2214	10	-0.979563802567192
2214	11	-0.768978292353296
2214	12	-0.7799227717213668
2214	13	-1.007659273755749
2214	14	-1.0456013427687907
2214	15	-1.4322665405698487
2214	16	-1.0399112021182262
2214	17	-1.111848328642644
2214	18	-1.143437497944639
2214	19	-2.8281826954827807
2214	20	-1.3562182703995447
2214	21	-1.7553362409375737
2214	22	-1.53234350789225
2214	23	-1.4817233751090626
2214	24	-0.7517608864656466
2214	25	-0.5586872874784348
2215	1	-1.2760948449228025
2215	2	-1.0711534051425033
2215	3	-1.0299092304938249
2215	4	-0.8303343247107051
2215	5	-1.5885235500079347
2215	6	-1.3036542052704263
2215	7	-1.5048667947199874
2215	8	-2.215981047944531
2215	9	-1.6219530737896264
2215	10	-1.182011353079453
2215	11	-1.7590629743011377
2215	12	-2.4963129681300344
2215	13	-1.9849708953640501
2215	14	-2.0323082616284083
2215	15	-3.137803246579395
2215	16	-2.519090997794592
2215	17	-2.3113254613011947
2215	18	-2.8726507009380384
2215	19	-3.4924284847771006
2215	20	-2.768804961398864
2215	21	-1.983321082581618
2215	22	-2.8700702658221715
2215	23	-2.8719408692624526
2215	24	-1.8135860355993358
2215	25	-1.9741305347065534
2216	1	-1.2308221701251973
2216	2	-1.1606567739658828
2216	3	-1.4806607720533123
2216	4	-1.065336540212627
2216	5	-1.0167892822531357
2216	6	-1.4175620306946968
2216	7	-2.828419276906285
2216	8	-2.0009576179209425
2216	9	-1.9180829426015293
2216	10	-1.7551587910962994
2216	11	-2.3977608279952776
2216	12	-1.8257907463710623
2216	13	-2.250490478235619
2216	14	-2.292341124098911
2216	15	-2.4914951195495334
2216	16	-2.1786239421950313
2216	17	-2.519895656191025
2216	18	-2.2113532732795917
2216	19	-2.4075543999442246
2216	20	-2.6634542091462734
2216	21	-2.5262250610154595
2216	22	-3.0063431872175883
2216	23	-2.060074149721082
2216	24	-2.3897332082730642
2216	25	-2.208875486965802
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	51324.0
3	2.0
4	797.0
5	7071.0
6	8037.0
7	6552.0
8	5405.0
9	4971.0
10	4119.0
11	3857.0
12	3662.0
13	3477.0
14	3488.0
15	3623.0
16	3652.0
17	3983.0
18	4458.0
19	5130.0
20	6649.0
21	8874.0
22	11303.0
23	13952.0
24	17048.0
25	20155.0
26	23402.0
27	25993.0
28	29895.0
29	36334.0
30	46461.0
31	59033.0
32	75714.0
33	97651.0
34	126744.0
35	165581.0
36	227157.0
37	352966.0
38	1169960.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.55806201515103	19.50596801381284	13.74642123996923	27.1895487310669
2	13.878211420692416	20.229041483827395	40.24947753891075	25.643269556569443
3	17.15612777053455	24.24483035687214	30.03357993996543	28.565461932627876
4	11.599622555815753	17.087348606250576	37.56365769042526	33.74937114750842
5	12.906096039262241	38.01507985572492	35.99826293412424	13.080561170888597
6	32.423306705284354	37.367657863265016	17.535254886982194	12.673780544468439
7	28.15891771919375	31.41979593871716	22.018652980181162	18.402633361907927
8	25.019447918077137	37.42393882259592	20.11895184919263	17.437661410134307
9	26.00576390732244	15.106215160593075	19.896666793068142	38.991354139016345
10	14.478933031396407	29.25986623050744	34.664868603935076	21.59633213416108
11	34.567549354111726	22.226121918660755	24.556868565658803	18.64946016156872
12	22.975531668500714	24.591073071416414	31.773653623889953	20.65974163619292
13	28.794040379958375	21.759822369725228	25.79675691328736	23.649380337029037
14	20.71891086883432	21.81946643428715	27.51373751854001	29.947885178338517
15	22.807101996631093	30.94543188575431	23.109158970891244	23.13830714672335
16	22.339731074979813	28.188414312846156	27.3029026767403	22.168951935433732
17	21.65593528337032	28.719009126130622	27.63802272604054	21.987032864458516
18	21.70595050800717	27.79809486413788	29.169052855713986	21.32690177214096
19	23.441850487083993	27.074873197455684	27.25206700844542	22.231209307014904
20	23.535948960996038	27.38600975781338	27.7114849887958	21.36655629239479
21	23.03234599419664	26.750444954503973	26.99698916645695	23.22021988484244
22	23.147902494932666	27.803634533373156	27.05615531437183	21.992307657322353
23	22.41191898365726	27.56291501167339	27.380309875382796	22.64485612928656
24	23.060672455607946	26.90682775947809	27.908920252918445	22.123579531995517
25	22.83950968230018	27.304348023837438	27.58978930559804	22.266352988264348
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	55.0
1	55.0
2	166.0
3	277.0
4	277.0
5	277.0
6	761.0
7	1245.0
8	1245.0
9	1245.0
10	2054.5
11	2864.0
12	2864.0
13	2864.0
14	5899.0
15	8934.0
16	8934.0
17	8934.0
18	17916.5
19	26899.0
20	26899.0
21	26899.0
22	44435.0
23	61971.0
24	61971.0
25	61971.0
26	90999.5
27	120028.0
28	120028.0
29	120028.0
30	156656.5
31	193285.0
32	193285.0
33	193285.0
34	222411.5
35	251538.0
36	251538.0
37	251538.0
38	275363.0
39	299188.0
40	299188.0
41	299188.0
42	316353.0
43	333518.0
44	333518.0
45	333518.0
46	339188.0
47	344858.0
48	344858.0
49	344858.0
50	334300.5
51	323743.0
52	323743.0
53	323743.0
54	290712.5
55	257682.0
56	257682.0
57	257682.0
58	221965.5
59	186249.0
60	186249.0
61	186249.0
62	151127.0
63	116005.0
64	116005.0
65	116005.0
66	90034.0
67	64063.0
68	64063.0
69	64063.0
70	46571.5
71	29080.0
72	29080.0
73	29080.0
74	20246.0
75	11412.0
76	11412.0
77	11412.0
78	7690.5
79	3969.0
80	3969.0
81	3969.0
82	2580.5
83	1192.0
84	1192.0
85	1192.0
86	784.0
87	376.0
88	376.0
89	376.0
90	208.5
91	41.0
92	41.0
93	41.0
94	23.5
95	6.0
96	6.0
97	6.0
98	4.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07928807495224523
2	2.2740365665079894E-4
3	0.0
4	0.02800855037749007
5	0.07011612746732968
6	0.07720354143294625
7	0.03558867226585003
8	0.07394408902095145
9	0.05230284102968376
10	0.11563475940693127
11	0.0037142597252963826
12	0.015804554137230527
13	0.0072390164033837665
14	0.010119462720960554
15	0.008944543828264758
16	0.0216033473818259
17	0.03369364179376005
18	0.001250720111579394
19	0.004775476789666778
20	0.006973712137291168
21	3.0320487553439857E-4
22	4.548073133015979E-4
23	0.0
24	3.790060944179982E-5
25	0.0041311664291561806
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2638480.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.06476573322995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.32566864553617	53.992830529902434
2	13.536030971411217	18.42653554053699
3	3.7573016742300784	7.672195747366289
4	1.4324055538441551	3.899853938295197
5	0.679542589557379	2.312645358198772
6	0.3660366311349916	1.4948518528790344
7	0.22069129292993486	1.051491080684775
8	0.14351624297978396	0.7814719565865843
9	0.09158686947992628	0.5610454933851982
>10	0.40921324687953015	4.716532979605525
>50	0.02165482844173447	1.0159616958135278
>100	0.013513674290406723	1.8043569200416876
>500	0.001780567394357866	0.8461606784904793
>1k	0.0010572118903487491	1.4240662282135919
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	3956	0.14993481095176012	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3548	0.13447136229950576	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3241	0.12283587520087323	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3177	0.12041023619659805	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3045	0.11540735575028047	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2689	0.10191473878899972	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.790060944179982E-5
7	0.0	0.0	0.0	0.0	3.790060944179982E-5
8	0.0	0.0	0.0	0.0	3.790060944179982E-5
9	0.0	0.0	0.0	1.1370182832539947E-4	7.580121888359964E-5
10	0.0	0.0	0.0	1.5160243776719928E-4	7.580121888359964E-5
11	0.0	0.0	0.0	1.8950304720899913E-4	7.580121888359964E-5
12	0.0	0.0	0.0	1.8950304720899913E-4	7.580121888359964E-5
13	0.0	0.0	0.0	2.2740365665079894E-4	7.580121888359964E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	840	0.0	13.935318	4
GTGCGCC	80	2.8949462E-5	11.864207	11
GGTATCA	935	0.0	11.796628	1
GTAGGAC	2345	0.0	11.394401	3
TGTAGGA	2405	0.0	11.267856	2
GGCGAGG	840	0.0	10.957774	19
GTATCAA	2015	0.0	10.806174	1
AGGACGT	2055	0.0	10.697721	5
GGACGTG	2080	0.0	10.611869	6
TGGCGAG	2025	0.0	10.590424	18
AGGACCT	1625	0.0	10.42456	5
CTGTAGG	2485	0.0	10.407681	1
TTAGGAC	1300	0.0	10.167155	3
TAGGACG	2200	0.0	10.165675	4
GGACCTG	1605	0.0	10.0772295	6
TCCAACG	180	4.0199666E-10	10.016385	18
GACGTGG	1120	0.0	10.013688	7
TAGGACA	765	0.0	9.952212	4
GTCCTAC	1965	0.0	9.919797	1
GTAGAAC	720	0.0	9.905052	3
>>END_MODULE