FastQCFastQC Report
Thu 26 May 2016
SRR1512989_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512989_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2949616
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46220.1566983634479878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG957.447852E-812.9929929
TAAGACG604.1038074E-412.663064
GCGTGCG907.5117496E-611.6048529
CGTGCGC1251.8488208E-811.39385510
TAGACCG600.005891736611.0798025
GGTATCA11050.011.035271
GTATCAA24250.010.6068711
GCGCCAC1659.258656E-1010.36701513
GTCCTAC8300.010.3299851
TAGAACA7500.09.8771874
GTCCTAA6150.09.6039931
TGCGCCA1606.608025E-89.50309812
AAGGCGT1901.0641088E-99.4947196
GGTCTAC3050.09.3703681
ACTGATC2151.07320375E-109.2740688
TTAGGAC7200.09.2334813
TACACCG1651.07178494E-79.2091855
GTATTAC2501.8189894E-129.145481
TCCAACG1054.4806272E-49.05041618
AAAGCCG1054.50883E-49.0447365
Produced by FastQC (version 0.11.5)