FastQCFastQC Report
Thu 26 May 2016
SRR1512989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512989_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2949616
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55260.18734642068662496No Hit
TATCAACGCAGAGTACTTTTTTTTT41010.13903504727395022No Hit
GGTATCAACGCAGAGTACTTTTTTT33340.1130316624265667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13000.013.8191411
TATAGCG550.003034463212.1094915
TAGACTG4600.010.5487975
TAGGACC4650.010.4353694
TTAGACG856.504195E-410.0742834
TGGCGAG8600.010.0411518
GTAGGAC9250.09.8662283
TATGGCG8650.09.76734316
GTATCAA26050.09.7059151
TTAGACT3550.09.6486084
GGCGAGG4250.09.37779919
ATGGCGA9550.09.34387517
CTAGTAC1951.6625563E-99.2627643
TAGGACG8500.09.1787914
AAGGCGT1356.744105E-69.1595616
TTTAGAC2700.09.15442853
CTAGACT3750.09.1340164
AGGACGT8600.09.0720615
CTAGACA3150.09.0615244
GTCTAGG3150.09.05261
Produced by FastQC (version 0.11.5)