FastQCFastQC Report
Thu 26 May 2016
SRR1512988_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512988_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3219346
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43440.13493423819620506No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGA550.003063546312.09413515
GTCCTAC11800.011.9485281
TAGGACC7100.011.90551954
GTGCGAT658.0588105E-411.68688611
GTATCAA25650.011.179281
CCGTGCG600.005898297811.0781969
TTAGGAC8500.011.0619823
GGTATCA11950.011.0013481
TAAACGT1002.407094E-510.4474284
TGGCGAG13100.010.445209518
AGGACGT11900.010.1356875
TAGGACG12400.010.033824
GTAGGAC15300.09.994273
CTAGTAC2201.8189894E-129.9293733
TGTAGGA16650.09.8684412
TATGGCG13800.09.84652316
GGACGTG12150.09.7677656
GGCGAGA8650.09.65950919
TCCTACA15550.09.6503562
GGTCTAC3950.09.6471181
Produced by FastQC (version 0.11.5)