##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512988_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3219346
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.01879418987583	31.0	30.0	34.0	23.0	34.0
2	29.19451310918429	31.0	30.0	34.0	22.0	34.0
3	29.326520355376527	31.0	30.0	34.0	23.0	34.0
4	32.80780754848966	35.0	33.0	37.0	26.0	37.0
5	32.92449864040709	35.0	35.0	37.0	26.0	37.0
6	32.946506526480846	37.0	35.0	37.0	26.0	37.0
7	32.78664051642787	37.0	33.0	37.0	25.0	37.0
8	32.765596801337914	37.0	35.0	37.0	25.0	37.0
9	34.11026369952158	39.0	35.0	39.0	25.0	39.0
10	33.99424852128352	39.0	34.0	39.0	25.0	39.0
11	34.051510772684885	38.0	35.0	39.0	25.0	39.0
12	33.94638880070673	38.0	34.0	39.0	24.0	39.0
13	33.94785431575233	39.0	35.0	39.0	24.0	39.0
14	34.94608252732077	39.0	35.0	41.0	24.0	41.0
15	34.87871667102573	39.0	34.0	41.0	23.0	41.0
16	34.76544770273217	39.0	34.0	41.0	22.0	41.0
17	34.70659972553432	39.0	34.0	41.0	21.0	41.0
18	34.40114265443975	39.0	34.0	41.0	17.0	41.0
19	34.64569574068771	39.0	34.0	41.0	18.0	41.0
20	34.71608550308044	39.0	34.0	41.0	19.0	41.0
21	34.6654165162738	39.0	34.0	41.0	19.0	41.0
22	34.57285610182938	39.0	34.0	41.0	18.0	41.0
23	34.30006933085167	39.0	34.0	41.0	16.0	41.0
24	34.298349726932116	39.0	34.0	41.0	16.0	41.0
25	34.21874908754759	39.0	34.0	41.0	15.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4845560388589085
1101	2	0.7690815038921954
1101	3	0.7356073143945103
1101	4	0.7225300544564348
1101	5	0.5288686147341792
1101	6	0.779415690088328
1101	7	0.8801664054020506
1101	8	0.6837188687651974
1101	9	0.5611570828307464
1101	10	0.6242042866286965
1101	11	0.8361183308095335
1101	12	0.9210782802815274
1101	13	0.8032552809058942
1101	14	0.7613721465911993
1101	15	0.5238241359387104
1101	16	0.9521133319639148
1101	17	1.0246774821234723
1101	18	1.2525013049804414
1101	19	0.6578094190156563
1101	20	0.8568854242715602
1101	21	0.9634922597394393
1101	22	0.9601505970621318
1101	23	1.0762952335002893
1101	24	0.8406614401724184
1101	25	1.0932683424202807
1102	1	0.49241354677981164
1102	2	0.5973356836624575
1102	3	0.5508514819064665
1102	4	0.7363310148865025
1102	5	0.5219046971142021
1102	6	0.5367218025331866
1102	7	0.7765923379278235
1102	8	0.6696808033849351
1102	9	0.6499913162816995
1102	10	0.8402520979525505
1102	11	0.8803629630667729
1102	12	0.8256173469528889
1102	13	0.8351912615902393
1102	14	0.931830860630555
1102	15	0.9930527925768047
1102	16	0.9647877583102726
1102	17	0.8600301239262649
1102	18	1.131830632496623
1102	19	1.0250355424003033
1102	20	1.0308927981865565
1102	21	0.836406780549197
1102	22	1.0957800136109768
1102	23	1.201469512981916
1102	24	1.1873785565062533
1102	25	0.9495904122077903
1103	1	0.32513526957761485
1103	2	0.5016394356567275
1103	3	0.47835087313179514
1103	4	0.5644785404522352
1103	5	0.14322791079595731
1103	6	0.5424101737892641
1103	7	0.37066355485274016
1103	8	0.31152201087284226
1103	9	0.6192562615995314
1103	10	0.6043579728969917
1103	11	0.6132759783562065
1103	12	0.7609080908329986
1103	13	0.7745156493202359
1103	14	0.8660707558940217
1103	15	0.9465079760864512
1103	16	0.9695208710132803
1103	17	0.8771535869608442
1103	18	1.174032401530802
1103	19	1.1637497268260404
1103	20	1.041038427360995
1103	21	0.8623700370450251
1103	22	0.9324511069028674
1103	23	1.3089760698502957
1103	24	1.1391937002517167
1103	25	1.0583481474985987
1104	1	0.4164163479424552
1104	2	0.4948366359413896
1104	3	0.4663879076615096
1104	4	0.6600317330072372
1104	5	0.36337801270035186
1104	6	0.41093506152380144
1104	7	0.6301047786081568
1104	8	0.5922027201472702
1104	9	0.6421898423874524
1104	10	0.6965546222429353
1104	11	0.7502472684499324
1104	12	0.6814510048860072
1104	13	0.6805327146606857
1104	14	0.8160156895419561
1104	15	0.9183110317323582
1104	16	0.8041366091473208
1104	17	0.8649740436840858
1104	18	1.1362006343274516
1104	19	0.9107483216846433
1104	20	0.8922291099470954
1104	21	0.8771526419118914
1104	22	0.9121183861517395
1104	23	1.2144080723996922
1104	24	1.0509917693793938
1104	25	0.8675428248031736
1105	1	0.6496314424983005
1105	2	0.7582232795426123
1105	3	0.7621425575140677
1105	4	0.8541044550060164
1105	5	0.613896194132991
1105	6	0.44004348392105896
1105	7	0.6607050146503397
1105	8	0.722960300799258
1105	9	0.7008549270949231
1105	10	0.8032878108069497
1105	11	0.827177031459648
1105	12	0.9161452017476464
1105	13	0.8680098454261156
1105	14	0.9730675498231136
1105	15	1.165326053619772
1105	16	1.1209356389495042
1105	17	0.9106741844378732
1105	18	0.9815611685271151
1105	19	0.9787572513420741
1105	20	0.9847383488943393
1105	21	0.922916067253162
1105	22	0.6479423956012056
1105	23	0.8944194682901525
1105	24	1.026649205087132
1105	25	1.0511198679381124
1106	1	0.5142323386502134
1106	2	0.6231782283273652
1106	3	0.6049646776101909
1106	4	0.6121555421617231
1106	5	0.5211749692694596
1106	6	0.5446158382902411
1106	7	0.35727639239657805
1106	8	0.579644828296999
1106	9	0.6604353546149184
1106	10	0.8064151416630239
1106	11	0.6409700532787568
1106	12	0.7474759907131272
1106	13	0.7189068400505576
1106	14	0.9042263189288562
1106	15	0.9610689413102733
1106	16	0.8494381361256629
1106	17	0.8325915401339401
1106	18	1.0378712804817525
1106	19	0.6257078250889094
1106	20	0.7316056553455681
1106	21	0.6898045272287803
1106	22	0.6929126724938826
1106	23	0.9719603274119066
1106	24	0.9549319237945895
1106	25	1.1466701813338744
1107	1	0.4102320485770612
1107	2	0.5858221318055215
1107	3	0.6730730607243061
1107	4	0.728123918144874
1107	5	0.6432482897912308
1107	6	0.6077612888252162
1107	7	0.7974344142789676
1107	8	0.7528784414300631
1107	9	0.8565827072441579
1107	10	0.7161237163462815
1107	11	0.7712436058590484
1107	12	0.8655384455780464
1107	13	0.8167019063896817
1107	14	1.0056611612469055
1107	15	1.172541447814261
1107	16	1.0578109768180894
1107	17	1.109922625400337
1107	18	1.3842295026959164
1107	19	1.3096620207251206
1107	20	1.2160197755627635
1107	21	1.232552131859073
1107	22	1.1747423466752522
1107	23	1.261324613967922
1107	24	1.2013230813979803
1107	25	1.192659466756794
1108	1	0.38805423887196255
1108	2	0.511065616442675
1108	3	0.43896853353592746
1108	4	0.5495777727631364
1108	5	0.5106776715596766
1108	6	0.6517347121265047
1108	7	0.6506785623086841
1108	8	0.6810183054532217
1108	9	0.8029969865827411
1108	10	0.772579168883432
1108	11	0.6613720077258662
1108	12	0.7308343494084824
1108	13	0.6899490173243805
1108	14	0.9556229136840813
1108	15	0.8512526027788496
1108	16	0.7952612573157509
1108	17	0.87589282304846
1108	18	0.9404739181324686
1108	19	0.839167429890189
1108	20	0.8845120762025829
1108	21	0.8356421249735817
1108	22	0.6658678871957733
1108	23	0.8531619038974938
1108	24	0.9038990384489054
1108	25	1.07171349877666
1109	1	0.2451870209189302
1109	2	0.17036176495530597
1109	3	0.33031045913984514
1109	4	0.4459216192188933
1109	5	0.27086557128856725
1109	6	0.2349345679776249
1109	7	0.2825332363626032
1109	8	0.41514823512019916
1109	9	0.5536925918172813
1109	10	0.6584922043468566
1109	11	0.1801694222630701
1109	12	0.4590826949638753
1109	13	0.5108571023918671
1109	14	0.31621557108154974
1109	15	0.555393521153789
1109	16	0.6276828565297308
1109	17	0.41561524579225306
1109	18	-1.0642856391914535
1109	19	0.24539454068348476
1109	20	0.36182131974959475
1109	21	0.4447748783116481
1109	22	0.6883118226868845
1109	23	0.26276227227796767
1109	24	0.6682179217634854
1109	25	0.6675558083620743
1110	1	0.2108373909668373
1110	2	0.16181738783673083
1110	3	0.26028454801277334
1110	4	0.08793112894002064
1110	5	0.3226276454832018
1110	6	0.25040911929193754
1110	7	0.3089255375763287
1110	8	0.1929518831551036
1110	9	0.4543940465641967
1110	10	0.7385558047307583
1110	11	0.5663971254405453
1110	12	0.04554213075884661
1110	13	0.27489876799991464
1110	14	0.36391199111407957
1110	15	0.5163437500234025
1110	16	0.7151026308188975
1110	17	0.24248288138010565
1110	18	0.7831015006531672
1110	19	0.6011268988008212
1110	20	0.5385851477706964
1110	21	0.4958983321477177
1110	22	0.5985388872819257
1110	23	0.5536887135725621
1110	24	0.7662111650759087
1110	25	0.8439006899001029
1111	1	0.03164234696924595
1111	2	0.33133528189143036
1111	3	0.2493996392751967
1111	4	0.10488638278357598
1111	5	0.20198133445244082
1111	6	0.3701741252193713
1111	7	0.22601229313779214
1111	8	0.01596723571967118
1111	9	0.28809266674346645
1111	10	0.2266968707723862
1111	11	0.1395043861449281
1111	12	0.3162983294350923
1111	13	0.3272113190868424
1111	14	0.25736122588311616
1111	15	0.4561641792270734
1111	16	0.35770033299940707
1111	17	0.3291202807625382
1111	18	0.6524247133936427
1111	19	0.3595953233089375
1111	20	0.25243555364354364
1111	21	0.4134585866911138
1111	22	0.18273950299299457
1111	23	0.5929497674886548
1111	24	0.6471546290036017
1111	25	0.03358756810886376
1112	1	-0.08418433477807596
1112	2	0.1460130025014017
1112	3	0.267172206617559
1112	4	0.2821015966923781
1112	5	0.2844306730070443
1112	6	0.29401343902000576
1112	7	-0.08536674504635045
1112	8	0.1891522562488177
1112	9	0.4740076867852494
1112	10	0.41480167668700574
1112	11	0.39256840909756363
1112	12	0.3871593192518503
1112	13	0.08395188476333004
1112	14	0.34831337054095
1112	15	0.29527559991216634
1112	16	0.5347908593391821
1112	17	0.5101905241691256
1112	18	0.5142928812901957
1112	19	0.39123036342731865
1112	20	0.18201144501725253
1112	21	0.23963477972013436
1112	22	0.2902875947351973
1112	23	0.5546488476488349
1112	24	0.24782275183541458
1112	25	0.45325760176184815
1113	1	-0.1587835579173067
1113	2	0.06562561998087801
1113	3	-0.17785120430525936
1113	4	0.12888038434286386
1113	5	-0.14969678710557588
1113	6	0.021381953556897315
1113	7	-0.08185017273186901
1113	8	0.196957476556463
1113	9	0.09680491967102967
1113	10	-0.21920739352821528
1113	11	-0.16918793404627763
1113	12	0.19783310398351261
1113	13	0.02545308463871976
1113	14	0.18289510890906513
1113	15	-0.2881937025906609
1113	16	-0.3183657660232413
1113	17	-0.174774679366422
1113	18	-0.2864958521454355
1113	19	-0.21540555595947808
1113	20	-0.33266163455952835
1113	21	-0.19719057916564253
1113	22	0.08751163083687175
1113	23	-0.3292509721153323
1113	24	0.17358221476489888
1113	25	0.203101048606527
1114	1	-1.4054473395518485
1114	2	-1.193961917072162
1114	3	-0.9581361564073951
1114	4	-1.2122119483740832
1114	5	-1.2720026238886426
1114	6	-1.696757369202384
1114	7	-1.81485471166188
1114	8	-1.3940795814787208
1114	9	-1.4387687997890453
1114	10	-1.5369722628877867
1114	11	-2.237367054968235
1114	12	-1.7342556951894537
1114	13	-1.6214525225041498
1114	14	-1.9851762836857532
1114	15	-1.9553647339948697
1114	16	-2.606783628904026
1114	17	-4.602214547702381
1114	18	-3.6150703844166223
1114	19	-2.5984793768005474
1114	20	-1.7651261996977468
1114	21	-2.705128054812164
1114	22	-2.779088885138517
1114	23	-2.665373030523714
1114	24	-2.2673509840729125
1114	25	-2.158585790102869
1115	1	-1.0248251980708751
1115	2	-0.6578380789739917
1115	3	-0.77680690918476
1115	4	-0.6964986940626048
1115	5	-0.5763784571391071
1115	6	-0.6364235273285601
1115	7	-1.622740848718795
1115	8	-1.0387854647828263
1115	9	-0.8503967923153652
1115	10	-0.6978956155682994
1115	11	-1.5100834534990497
1115	12	-1.1643651728803732
1115	13	-0.9375940746279312
1115	14	-0.943320410043107
1115	15	-2.1516780984227424
1115	16	-0.9767270264880992
1115	17	-1.1306797175298442
1115	18	-1.1560175956501126
1115	19	-1.174828161194739
1115	20	-0.8876991315994047
1115	21	-1.7773029264440936
1115	22	-1.8964517025013379
1115	23	-1.1712308671811513
1115	24	-1.0619858010670598
1115	25	-1.4661777983365383
1116	1	-1.3166963770450728
1116	2	-2.0576789098760138
1116	3	-1.6398805666038854
1116	4	-1.2716669191146437
1116	5	-1.8362686374398187
1116	6	-1.6745898536436101
1116	7	-1.2659613172684665
1116	8	-1.848567290831113
1116	9	-1.9809084743375038
1116	10	-2.9495437674875546
1116	11	-1.598148086683743
1116	12	-1.4790402274857186
1116	13	-2.09673240456409
1116	14	-1.9566821451321843
1116	15	-1.4354011391171255
1116	16	-1.3229951487050968
1116	17	-1.6014256553555626
1116	18	-1.5418267729000306
1116	19	-1.875199788367297
1116	20	-2.242258606220844
1116	21	-2.7018523966945693
1116	22	-1.8521330369588114
1116	23	-1.8237117227991106
1116	24	-2.465314522366345
1116	25	-2.242602786806451
1201	1	0.2063330127010694
1201	2	0.224882861686865
1201	3	0.41427348897718375
1201	4	0.357231475547394
1201	5	0.46035794762305215
1201	6	0.42798202420145515
1201	7	0.36405784730736457
1201	8	-0.13172247402884807
1201	9	0.14562984856106453
1201	10	0.38124178223863225
1201	11	-0.02224506330014009
1201	12	-0.6234158851499743
1201	13	-0.29855588086870455
1201	14	0.29681856400144824
1201	15	0.12412457008378652
1201	16	0.6283880378544353
1201	17	0.6477308484343709
1201	18	0.8175584551791601
1201	19	0.38653174673169133
1201	20	0.32615410907786924
1201	21	0.31462916226911375
1201	22	0.4783400950372467
1201	23	0.8150281469748535
1201	24	0.6622842161214137
1201	25	0.4396540549202115
1202	1	0.47428733158827896
1202	2	0.6107428375168951
1202	3	0.3062805263946373
1202	4	0.6268078044850469
1202	5	0.5676710476419089
1202	6	0.5916165194413878
1202	7	0.5448045650082918
1202	8	0.6127607942795095
1202	9	0.9311628841398161
1202	10	0.9955479010263488
1202	11	0.7039076678473819
1202	12	0.6919577899538396
1202	13	0.679185961766251
1202	14	0.8844529018539973
1202	15	0.52106721339009
1202	16	0.8210395174601217
1202	17	1.005667788994515
1202	18	1.2745625387081603
1202	19	1.0447040537152859
1202	20	0.8653447994991907
1202	21	0.882548389355776
1202	22	0.9570847483019946
1202	23	1.1859594859899047
1202	24	1.2360557730999204
1202	25	1.0657452932730536
1203	1	0.22329804213107352
1203	2	0.427281395146494
1203	3	0.5412983394452127
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2211	16	-0.11639325706342163
2211	17	0.027876713753862248
2211	18	0.08218932437498694
2211	19	-0.1700763609883964
2211	20	-0.2608896184262548
2211	21	-0.23339863253980297
2211	22	-0.42540923145886467
2211	23	0.24727323314112937
2211	24	0.03556836466892577
2211	25	0.020279438120702764
2212	1	0.12658862053167041
2212	2	0.09399777838261869
2212	3	0.14184424713280208
2212	4	0.058525782966569295
2212	5	0.10963205326451231
2212	6	0.07691384008084867
2212	7	-0.07158039446515829
2212	8	0.15017877175384342
2212	9	0.17392390839531657
2212	10	0.22982387440647756
2212	11	0.04324219007718
2212	12	0.2832492387121093
2212	13	0.11435732148947864
2212	14	0.1962645547180486
2212	15	0.041016375790917436
2212	16	0.25085659400860294
2212	17	0.18111636345182802
2212	18	-2.361979786929453
2212	19	-0.6862172109549718
2212	20	-0.2118095665164148
2212	21	-0.1873457556333591
2212	22	0.1272541062475412
2212	23	-0.04510105330662384
2212	24	-0.4675127418328415
2212	25	-0.05630444523686862
2213	1	-0.055277941723616664
2213	2	0.058868882532969735
2213	3	-0.25880821958607214
2213	4	-0.30292981609373015
2213	5	-0.2257530681137183
2213	6	-0.23920534551037775
2213	7	-0.09284977582772314
2213	8	-0.49676171375907785
2213	9	-0.4054908136093829
2213	10	-0.3448636745363558
2213	11	-0.4185897598053572
2213	12	-0.4279579656731656
2213	13	-0.24747384530770233
2213	14	-0.23012041004310646
2213	15	-0.4808273203719651
2213	16	-0.9350148233758944
2213	17	-0.46017979942992326
2213	18	-0.3209268503593705
2213	19	-0.3216248851914685
2213	20	-1.0397317278319989
2213	21	-0.6189795849207513
2213	22	-0.5576888851385178
2213	23	-0.963213504363793
2213	24	-0.7247197896010462
2213	25	-0.8600157999745406
2214	1	-1.4738165374859769
2214	2	-3.416314499270104
2214	3	-1.6452680450950012
2214	4	-1.2427092926205248
2214	5	-1.328296150789246
2214	6	-1.3253988644135788
2214	7	-1.5539004613283467
2214	8	-1.2048784565924784
2214	9	-1.4152097168083841
2214	10	-1.4700959138383922
2214	11	-1.2539676576034395
2214	12	-1.7629327891048234
2214	13	-1.528070272395361
2214	14	-2.7038106468524106
2214	15	-2.9099796261467894
2214	16	-2.2690448192213424
2214	17	-2.6373824583414347
2214	18	-1.9726296339073528
2214	19	-4.20075675058407
2214	20	-2.552793381342596
2214	21	-2.3770965770271104
2214	22	-2.383825071262322
2214	23	-2.3466353572927474
2214	24	-2.6785837696592125
2214	25	-2.438837819085464
2215	1	-0.8390752640717132
2215	2	-2.498699809537058
2215	3	-1.2513173853945148
2215	4	-0.9025668912739562
2215	5	-0.7511532740869384
2215	6	-1.3140286204846348
2215	7	-1.5609208365898262
2215	8	-1.6464640824511463
2215	9	-1.2654601235990839
2215	10	-2.74486573426859
2215	11	-1.8981096362214203
2215	12	-2.0461383982169394
2215	13	-1.5137836290358138
2215	14	-2.8257096994354853
2215	15	-2.6651824182092483
2215	16	-2.2352008171966986
2215	17	-2.4845674410365177
2215	18	-1.3755054291441269
2215	19	-2.126064843996822
2215	20	-2.2082880615086253
2215	21	-1.9049350947044772
2215	22	-1.566318545282698
2215	23	-2.1316884786171357
2215	24	-1.9054631469645926
2215	25	-1.7836242448766981
2216	1	-1.8734491824795754
2216	2	-1.9446877320931577
2216	3	-2.574266559766844
2216	4	-3.291413957424872
2216	5	-2.3638115726330255
2216	6	-2.429132872215394
2216	7	-2.2106961192952674
2216	8	-2.77009532098997
2216	9	-3.077524831686624
2216	10	-2.503371480616881
2216	11	-3.023456316912995
2216	12	-3.6253995433231267
2216	13	-4.028117723697182
2216	14	-3.3301322424178004
2216	15	-3.2807760467483007
2216	16	-2.547443911297009
2216	17	-2.6437630368991094
2216	18	-2.598689052495768
2216	19	-2.9569596427921248
2216	20	-2.873824907782698
2216	21	-3.2050893630637276
2216	22	-3.682005894177138
2216	23	-3.6738613268781712
2216	24	-3.664529649913291
2216	25	-3.7157615682572
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	149384.0
3	2.0
4	1253.0
5	7340.0
6	9090.0
7	8303.0
8	7254.0
9	7052.0
10	6388.0
11	6139.0
12	5944.0
13	5894.0
14	5898.0
15	5929.0
16	6240.0
17	6600.0
18	7455.0
19	8801.0
20	11342.0
21	14752.0
22	18420.0
23	22385.0
24	26227.0
25	29969.0
26	33605.0
27	37453.0
28	42361.0
29	50221.0
30	61977.0
31	77384.0
32	97406.0
33	124406.0
34	160554.0
35	209857.0
36	284474.0
37	438566.0
38	1223021.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.41971334824525	18.452953417141433	12.858115954453703	25.269217280159616
2	14.942658390929752	19.701455711818404	41.16051113675786	24.195374760493984
3	18.681413608335582	24.043441621475832	29.50338138912263	27.771763381065963
4	12.19619056260328	16.457586154822327	38.51749632871359	32.8287269538608
5	13.37905270229873	37.59029570191527	35.83559299175461	13.1950586040314
6	32.222522890018865	37.05270444124307	17.99984528208214	12.72492738665592
7	28.10115445042363	31.575317334837845	22.131000095999674	18.192528118738856
8	25.128946496020145	36.64988599020128	20.469162982592028	17.75200453118655
9	26.138046995779625	15.426393245413013	20.030869874283557	38.404689884523805
10	14.503899089694597	29.104700655544164	34.65349364650325	21.737906608257994
11	34.51435924823696	22.676635825281238	24.17477468576863	18.634230240713176
12	22.560122931732934	24.851081043043195	31.793704098273306	20.795091926950565
13	28.48974506748853	21.73930739010686	25.89300943394761	23.877938108457
14	20.99924063155592	21.442371267784882	27.930372997802678	29.62801510285652
15	23.163594118529836	29.866640649978077	23.49888004991227	23.470885181579813
16	22.778138177100228	28.246669723847127	26.63263999960232	22.34255209945033
17	22.02102086894005	28.209684239567938	27.415552858049637	22.353742033442376
18	21.933839865546638	27.89717613732651	28.502496124826454	21.6664878723004
19	23.272168241854967	27.285364217567064	27.436705330991494	22.00576220958647
20	23.318354859646327	27.30705311812302	27.749975999023185	21.62461602320747
21	23.29548261453009	26.877220934708827	27.163159724606427	22.664136726154652
22	23.00174201251378	27.4020712146646	27.365037526124752	22.231149246696873
23	22.847048903302255	27.209450518654442	27.226445395646635	22.717055182396663
24	22.813873794943966	26.854970749332814	27.86943918874836	22.46171626697486
25	22.955710266250424	27.07483073512159	27.491437334711975	22.47802166391601
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	217.0
1	217.0
2	472.0
3	727.0
4	727.0
5	727.0
6	1523.5
7	2320.0
8	2320.0
9	2320.0
10	3825.0
11	5330.0
12	5330.0
13	5330.0
14	9855.5
15	14381.0
16	14381.0
17	14381.0
18	26082.5
19	37784.0
20	37784.0
21	37784.0
22	58718.5
23	79653.0
24	79653.0
25	79653.0
26	111188.5
27	142724.0
28	142724.0
29	142724.0
30	181954.5
31	221185.0
32	221185.0
33	221185.0
34	256255.5
35	291326.0
36	291326.0
37	291326.0
38	322441.0
39	353556.0
40	353556.0
41	353556.0
42	375694.0
43	397832.0
44	397832.0
45	397832.0
46	408713.0
47	419594.0
48	419594.0
49	419594.0
50	410482.0
51	401370.0
52	401370.0
53	401370.0
54	364806.5
55	328243.0
56	328243.0
57	328243.0
58	282654.5
59	237066.0
60	237066.0
61	237066.0
62	192981.5
63	148897.0
64	148897.0
65	148897.0
66	114682.5
67	80468.0
68	80468.0
69	80468.0
70	58190.5
71	35913.0
72	35913.0
73	35913.0
74	24988.5
75	14064.0
76	14064.0
77	14064.0
78	9403.5
79	4743.0
80	4743.0
81	4743.0
82	3078.0
83	1413.0
84	1413.0
85	1413.0
86	907.0
87	401.0
88	401.0
89	401.0
90	249.5
91	98.0
92	98.0
93	98.0
94	62.5
95	27.0
96	27.0
97	27.0
98	20.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.36097393694247215
2	0.0392626328453046
3	0.035473043282704
4	0.03503817234929082
5	0.04062937006460318
6	0.018171392574765185
7	0.018171392574765185
8	0.023700465871018525
9	0.020935929222891857
10	0.020066187356065485
11	0.01922750769876863
12	0.020159373984654026
13	0.020221498403713054
14	0.02739686880503059
15	0.027924926367032313
16	0.021557173413482116
17	0.022830724004192157
18	0.10440008622869365
19	0.024508083318785866
20	0.02258222632795605
21	0.025626322861848336
22	0.020345747241831105
23	0.02264435074701508
24	0.019134321070180094
25	0.022426915280308487
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3219346.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.49528372282786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.86023499318125	55.49909689022837
2	12.848118674267061	17.85767305145505
3	3.8619880529577646	8.051698664234145
4	1.442420616878997	4.009657200706491
5	0.7229972001941668	2.51224477791519
6	0.35979996657226543	1.500264045624214
7	0.2257608761947096	1.0982521301265888
8	0.1403744500317991	0.7804289785916642
9	0.10211191880229387	0.6386667091782996
>10	0.40725032424995933	4.718405053692185
>50	0.018737446750744537	0.8970638839297381
>100	0.008638810993899095	1.1748301381674886
>500	9.8476332639374E-4	0.49756378387715267
>1k	5.819055987603252E-4	0.7641546922734378
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4344	0.13493423819620506	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.106220952951314E-5
2	0.0	0.0	0.0	0.0	3.106220952951314E-5
3	0.0	0.0	0.0	0.0	3.106220952951314E-5
4	0.0	0.0	0.0	0.0	3.106220952951314E-5
5	0.0	0.0	0.0	0.0	3.106220952951314E-5
6	0.0	0.0	0.0	0.0	3.106220952951314E-5
7	0.0	0.0	0.0	0.0	3.106220952951314E-5
8	0.0	0.0	0.0	0.0	3.106220952951314E-5
9	0.0	0.0	0.0	6.212441905902628E-5	3.106220952951314E-5
10	3.106220952951314E-5	0.0	0.0	1.2424883811805256E-4	3.106220952951314E-5
11	3.106220952951314E-5	0.0	0.0	1.2424883811805256E-4	3.106220952951314E-5
12	3.106220952951314E-5	0.0	0.0	1.2424883811805256E-4	1.5531104764756568E-4
13	3.106220952951314E-5	0.0	0.0	1.2424883811805256E-4	2.1743546670659197E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTCGA	55	0.0030635463	12.094135	15
GTCCTAC	1180	0.0	11.948528	1
TAGGACC	710	0.0	11.9055195	4
GTGCGAT	65	8.0588105E-4	11.686886	11
GTATCAA	2565	0.0	11.17928	1
CCGTGCG	60	0.0058982978	11.078196	9
TTAGGAC	850	0.0	11.061982	3
GGTATCA	1195	0.0	11.001348	1
TAAACGT	100	2.407094E-5	10.447428	4
TGGCGAG	1310	0.0	10.4452095	18
AGGACGT	1190	0.0	10.135687	5
TAGGACG	1240	0.0	10.03382	4
GTAGGAC	1530	0.0	9.99427	3
CTAGTAC	220	1.8189894E-12	9.929373	3
TGTAGGA	1665	0.0	9.868441	2
TATGGCG	1380	0.0	9.846523	16
GGACGTG	1215	0.0	9.767765	6
GGCGAGA	865	0.0	9.659509	19
TCCTACA	1555	0.0	9.650356	2
GGTCTAC	395	0.0	9.647118	1
>>END_MODULE