FastQCFastQC Report
Thu 26 May 2016
SRR1512988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512988_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3219346
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55070.17105958787902883No Hit
TATCAACGCAGAGTACTTTTTTTTT39350.1222297944986342No Hit
GGTATCAACGCAGAGTACTTTTTTT33110.10284697575221799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13750.012.5151341
TAGGACC6450.011.8017994
TACACCG1552.5465852E-1111.6637945
GGCGAGG5600.011.52366219
CGTGCGC1604.5474735E-1111.28243210
CTAGACT3600.011.1010674
ACACCGT1803.45608E-1110.5693176
TCCAACG1803.6379788E-1110.54419918
TGGCGAG12150.010.46609418
GTAGGAC14200.010.1770433
GTATCAA27450.010.1134691
GACTGCG856.568099E-410.0636337
GTCCTAC13450.010.0375031
ATGGCGA12500.010.02354217
TATGGCG12300.09.95501516
GTCCTAG3850.09.8777691
TGTAGGA16050.09.773612
CTACACT6050.09.7511154
GGACGTG12750.09.6989036
TCCTACA16350.09.6524252
Produced by FastQC (version 0.11.5)