##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512988_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3219346 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.686860312622503 31.0 31.0 34.0 27.0 34.0 2 30.814395532508776 31.0 31.0 34.0 27.0 34.0 3 30.907462882212723 31.0 31.0 34.0 27.0 34.0 4 34.41158483741729 37.0 35.0 37.0 31.0 37.0 5 34.09945591433788 37.0 35.0 37.0 30.0 37.0 6 33.99106215982998 37.0 35.0 37.0 30.0 37.0 7 33.880293699403545 37.0 35.0 37.0 29.0 37.0 8 33.77591380361105 37.0 35.0 37.0 29.0 37.0 9 35.25627844910115 39.0 35.0 39.0 29.0 39.0 10 35.1470099827729 39.0 35.0 39.0 28.0 39.0 11 35.19467804951689 39.0 35.0 39.0 29.0 39.0 12 35.04209612759859 39.0 35.0 39.0 27.0 39.0 13 35.04210451439516 39.0 35.0 39.0 27.0 39.0 14 36.12649556773332 40.0 36.0 41.0 27.0 41.0 15 36.13996600551789 40.0 36.0 41.0 27.0 41.0 16 36.11982620072524 40.0 36.0 41.0 27.0 41.0 17 36.090443835487086 40.0 36.0 41.0 27.0 41.0 18 36.015261795408136 40.0 36.0 41.0 27.0 41.0 19 36.007682616282935 40.0 36.0 41.0 27.0 41.0 20 35.97472902881517 40.0 36.0 41.0 27.0 41.0 21 35.92516368231312 40.0 36.0 41.0 27.0 41.0 22 35.8855916698609 40.0 36.0 41.0 27.0 41.0 23 35.79239230576645 40.0 36.0 41.0 27.0 41.0 24 35.775310264879884 40.0 36.0 41.0 27.0 41.0 25 35.68456450471618 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44024606226416196 1101 2 0.5090160657352847 1101 3 0.4774662322334571 1101 4 0.22434513292171232 1101 5 0.3971920451320017 1101 6 0.4692167223007999 1101 7 0.4938899914240835 1101 8 0.45925655739395665 1101 9 0.35318959619957724 1101 10 0.6119092139042976 1101 11 0.678493296301184 1101 12 0.6567650668148204 1101 13 0.763270294092095 1101 14 0.8475924094157818 1101 15 0.8436069123757335 1101 16 0.969735957136507 1101 17 0.8765825600995072 1101 18 0.3268643961089026 1101 19 0.683626831068608 1101 20 0.6295088882091164 1101 21 0.7810932509275688 1101 22 0.6651272071708192 1101 23 0.6143003561511549 1101 24 0.6362580301934244 1101 25 0.6622008289391204 1102 1 0.538141604886917 1102 2 0.45872085130624995 1102 3 0.43685699163508573 1102 4 0.3417821703793962 1102 5 0.43476542854293854 1102 6 0.42686256550670265 1102 7 0.5366604281410545 1102 8 0.5236126828281868 1102 9 0.6633519992199055 1102 10 0.6824281914385608 1102 11 0.6173757829756568 1102 12 0.68768335649996 1102 13 0.7737501533891518 1102 14 0.7647880338911435 1102 15 0.8089966065185052 1102 16 0.7860125837123633 1102 17 0.7280749831848112 1102 18 0.8338086501731397 1102 19 0.8178976672576397 1102 20 0.8696965291176681 1102 21 0.8541907651930245 1102 22 0.7924917293513118 1102 23 0.8227892029170647 1102 24 0.7944730395079134 1102 25 0.8857726836513891 1103 1 0.4017323368724455 1103 2 0.2860636867146056 1103 3 0.35425080359506467 1103 4 0.4357988905342296 1103 5 0.3987224366108748 1103 6 0.505409426388411 1103 7 0.4071492954702265 1103 8 0.4317761545180403 1103 9 0.5041813806679158 1103 10 0.538347640236239 1103 11 0.5301831981320504 1103 12 0.5867579066367483 1103 13 0.5615854790783885 1103 14 0.6473676397069354 1103 15 0.4716123198555451 1103 16 0.5413089233433013 1103 17 0.6800240011754894 1103 18 0.6892262310719488 1103 19 0.8624201639898388 1103 20 0.8474289401847983 1103 21 0.5285810715419288 1103 22 0.7801122944258339 1103 23 0.759522225086414 1103 24 0.7503399659398369 1103 25 0.7763000695223923 1104 1 0.3668110231395403 1104 2 0.4569576109228315 1104 3 0.44572699462164067 1104 4 0.39267128685344943 1104 5 0.482138465790257 1104 6 0.5010137500189487 1104 7 0.5240581054811315 1104 8 0.3779638622552355 1104 9 0.6095317606284993 1104 10 0.589455599456862 1104 11 0.7513132664517528 1104 12 0.7415064644817306 1104 13 0.7683542647951498 1104 14 0.8497283653168921 1104 15 0.8501985813923696 1104 16 0.8635266140349671 1104 17 0.8012704215874109 1104 18 0.7931367951704473 1104 19 0.9225919181710012 1104 20 0.90532236188848 1104 21 0.8061707999748222 1104 22 0.8820998017115969 1104 23 0.6829832980464374 1104 24 0.750553791804677 1104 25 0.8282027894864612 1105 1 0.4287917459855848 1105 2 0.49439378746397367 1105 3 0.4619193747919077 1105 4 0.414223770515008 1105 5 0.3665738508464571 1105 6 0.5369934092329984 1105 7 0.5942070104614601 1105 8 0.6803829467099547 1105 9 0.763466738358197 1105 10 0.5936269789399802 1105 11 0.6668175744489773 1105 12 0.57543950480121 1105 13 0.651796765985523 1105 14 0.8571392315215718 1105 15 0.7973728604213477 1105 16 0.8379357340437323 1105 17 0.5808800964014225 1105 18 0.8137073181658678 1105 19 0.9208511644165824 1105 20 0.8925593222022457 1105 21 0.7909022659385769 1105 22 0.8546354957932749 1105 23 0.7929854776329393 1105 24 0.8720829402464148 1105 25 0.9075315748037269 1106 1 0.4088193163548368 1106 2 0.3365385228625897 1106 3 0.3664376655577186 1106 4 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0.7217104281468352 1107 17 0.7308098082859829 1107 18 0.8902237983293873 1107 19 0.9276731665144453 1107 20 0.8817247436170419 1107 21 0.8209725273393857 1107 22 0.8378841214683703 1107 23 0.8305828167025382 1107 24 0.85799467165441 1107 25 0.8538041675144825 1108 1 0.3335293363751255 1108 2 0.28743079485123957 1108 3 0.31924061832599193 1108 4 0.3369153137597749 1108 5 0.36758295939479524 1108 6 0.28425873754006403 1108 7 0.35360688985073097 1108 8 0.41881840513185153 1108 9 0.3825560800696479 1108 10 0.49254921185249856 1108 11 0.45983836685508095 1108 12 0.5201717053158887 1108 13 0.5527355907168854 1108 14 0.5260745214626326 1108 15 0.6335197575675267 1108 16 0.5728507272884329 1108 17 0.5770704451614748 1108 18 0.61097484815744 1108 19 0.5583429600707817 1108 20 0.6105015390396318 1108 21 0.6774149356407335 1108 22 0.6533463858918012 1108 23 0.6317765506243518 1108 24 0.6895067501508976 1108 25 0.6225339664189562 1109 1 0.159952627147522 1109 2 0.13322760461286975 1109 3 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-1.986728650381643 2216 15 -1.9902433028864763 2216 16 -1.8166468798572168 2216 17 -2.0520938831146154 2216 18 -1.744661758449098 2216 19 -1.8881880128496746 2216 20 -2.386464591592933 2216 21 -2.1560432697224456 2216 22 -2.5471038480261896 2216 23 -1.629775611863991 2216 24 -1.9053941770752303 2216 25 -1.7493448840321904 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67206.0 3 2.0 4 1055.0 5 9486.0 6 10945.0 7 8658.0 8 7066.0 9 6370.0 10 5545.0 11 5190.0 12 4738.0 13 4489.0 14 4477.0 15 4661.0 16 4695.0 17 4997.0 18 5514.0 19 6471.0 20 8323.0 21 10841.0 22 14033.0 23 17346.0 24 20951.0 25 24578.0 26 28368.0 27 31365.0 28 35798.0 29 43075.0 30 55185.0 31 70511.0 32 90479.0 33 116944.0 34 152173.0 35 199886.0 36 275664.0 37 431645.0 38 1430616.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.770337837585785 19.31670237489788 13.778610624431892 27.134349163084444 2 13.882654866322019 20.389576624532786 39.720209819338805 26.00755868980639 3 17.111177239103842 24.219173707951864 30.02849025858047 28.641158794363825 4 11.569223350353171 17.091872641103457 37.65177179599435 33.687132212549024 5 13.011798161110768 37.819767965975146 35.956383422517405 13.212050450396681 6 32.237107575572 37.577928420565705 17.53676244290016 12.648201560962145 7 27.956020299470634 31.55870402571366 22.128778340270934 18.356497334544773 8 25.137478990760755 36.9873271585002 20.174651395057005 17.700542455682044 9 25.862732855747456 15.24795110561514 19.86331277109722 39.02600326754019 10 14.144703903074632 29.42885930459282 34.68070417673455 21.745732615597994 11 34.57950631458095 22.570681805756433 24.087759255669702 18.762052623992922 12 22.408056055633136 25.031866030640476 31.708525619418786 20.851552294307606 13 28.393210937699003 21.633907786397646 26.01291385564887 23.95996742025449 14 20.906468218379683 21.43875836450397 27.837050562748882 29.817722854367467 15 23.020785118960713 30.007455430504514 23.503679565596915 23.468079884937858 16 22.716114971347263 28.318878807225868 26.55637420523408 22.408632016192797 17 21.90360661870602 28.332587087787374 27.43199662682513 22.331809666681476 18 21.875447493987078 27.95201590896454 28.451345425132104 21.721191171916278 19 23.137515589883932 27.32015525427625 27.498147851896494 22.04418130394333 20 23.254370577507373 27.277609913279615 27.762296001145625 21.705723508067383 21 23.167906932390117 26.811358984784757 27.255736196614393 22.764997886210733 22 22.89685922030933 27.303741650431395 27.463806111089024 22.33559301817025 23 22.61450698822276 27.12343659968099 27.417005633133446 22.845050778962804 24 22.676545283759673 26.95459074892276 27.899876496578184 22.46898747073938 25 22.82363802354409 27.05026066735689 27.587561897430973 22.538539411668044 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 118.0 1 118.0 2 307.5 3 497.0 4 497.0 5 497.0 6 1487.5 7 2478.0 8 2478.0 9 2478.0 10 4085.5 11 5693.0 12 5693.0 13 5693.0 14 10180.5 15 14668.0 16 14668.0 17 14668.0 18 26346.5 19 38025.0 20 38025.0 21 38025.0 22 59349.5 23 80674.0 24 80674.0 25 80674.0 26 112536.5 27 144399.0 28 144399.0 29 144399.0 30 183476.0 31 222553.0 32 222553.0 33 222553.0 34 257168.0 35 291783.0 36 291783.0 37 291783.0 38 323060.5 39 354338.0 40 354338.0 41 354338.0 42 376864.0 43 399390.0 44 399390.0 45 399390.0 46 410281.0 47 421172.0 48 421172.0 49 421172.0 50 411089.5 51 401007.0 52 401007.0 53 401007.0 54 363973.0 55 326939.0 56 326939.0 57 326939.0 58 281219.0 59 235499.0 60 235499.0 61 235499.0 62 191631.0 63 147763.0 64 147763.0 65 147763.0 66 113072.0 67 78381.0 68 78381.0 69 78381.0 70 56463.5 71 34546.0 72 34546.0 73 34546.0 74 23936.5 75 13327.0 76 13327.0 77 13327.0 78 8872.5 79 4418.0 80 4418.0 81 4418.0 82 2847.0 83 1276.0 84 1276.0 85 1276.0 86 801.5 87 327.0 88 327.0 89 327.0 90 194.0 91 61.0 92 61.0 93 61.0 94 37.5 95 14.0 96 14.0 97 14.0 98 7.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07871163894778628 2 1.553110476475657E-4 3 0.0 4 0.029353788005389913 5 0.07467355170894958 6 0.08113449129108831 7 0.0352245456064679 8 0.07172264180364583 9 0.05078671258075398 10 0.11418468223049029 11 0.004038087238836708 12 0.012455946021334768 13 0.005218451200958207 14 0.007361743658494613 15 0.0067094372583748375 16 0.01960025421312279 17 0.03146601825339681 18 9.00804076355881E-4 19 0.0034479052577759584 20 0.004628269219897457 21 2.7955988576561827E-4 22 5.591197715312365E-4 23 3.106220952951313E-5 24 6.212441905902627E-5 25 0.0033857808387169324 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3219346.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.78218260208227 #Duplication Level Percentage of deduplicated Percentage of total 1 78.28565534445248 53.06372585681356 2 13.827107008199082 18.744629841765633 3 4.170556395204985 8.48068245396199 4 1.5963883555489258 4.328267480786206 5 0.7296577428456819 2.4728897181294602 6 0.39149739095472313 1.59219285851591 7 0.24621868753135875 1.1682468026806918 8 0.16077561017051056 0.8718177413230995 9 0.11295745579750313 0.6890852605619695 >10 0.4494935448867349 5.063418459243916 >50 0.01875734498364161 0.8714770458311587 >100 0.009286669645932855 1.2451402049932867 >500 9.615957067537823E-4 0.44560747734977435 >1k 6.410638001900555E-4 0.7918944848097205 >5k 4.5790271442146816E-5 0.17092431323379834 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5507 0.17105958787902883 No Hit TATCAACGCAGAGTACTTTTTTTTT 3935 0.1222297944986342 No Hit GGTATCAACGCAGAGTACTTTTTTT 3311 0.10284697575221799 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.106220952951314E-5 2 0.0 0.0 0.0 0.0 3.106220952951314E-5 3 0.0 0.0 0.0 3.106220952951314E-5 3.106220952951314E-5 4 0.0 0.0 0.0 3.106220952951314E-5 3.106220952951314E-5 5 0.0 0.0 0.0 3.106220952951314E-5 6.212441905902628E-5 6 0.0 0.0 0.0 3.106220952951314E-5 6.212441905902628E-5 7 0.0 0.0 0.0 3.106220952951314E-5 6.212441905902628E-5 8 0.0 0.0 0.0 3.106220952951314E-5 6.212441905902628E-5 9 0.0 0.0 0.0 6.212441905902628E-5 6.212441905902628E-5 10 0.0 0.0 0.0 9.318662858853941E-5 9.318662858853941E-5 11 0.0 0.0 0.0 1.2424883811805256E-4 9.318662858853941E-5 12 0.0 3.106220952951314E-5 0.0 1.2424883811805256E-4 2.795598857656182E-4 13 0.0 3.106220952951314E-5 0.0 1.2424883811805256E-4 3.416843048246445E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1375 0.0 12.515134 1 TAGGACC 645 0.0 11.801799 4 TACACCG 155 2.5465852E-11 11.663794 5 GGCGAGG 560 0.0 11.523662 19 CGTGCGC 160 4.5474735E-11 11.282432 10 CTAGACT 360 0.0 11.101067 4 ACACCGT 180 3.45608E-11 10.569317 6 TCCAACG 180 3.6379788E-11 10.544199 18 TGGCGAG 1215 0.0 10.466094 18 GTAGGAC 1420 0.0 10.177043 3 GTATCAA 2745 0.0 10.113469 1 GACTGCG 85 6.568099E-4 10.063633 7 GTCCTAC 1345 0.0 10.037503 1 ATGGCGA 1250 0.0 10.023542 17 TATGGCG 1230 0.0 9.955015 16 GTCCTAG 385 0.0 9.877769 1 TGTAGGA 1605 0.0 9.77361 2 CTACACT 605 0.0 9.751115 4 GGACGTG 1275 0.0 9.698903 6 TCCTACA 1635 0.0 9.652425 2 >>END_MODULE