FastQCFastQC Report
Thu 26 May 2016
SRR1512987_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512987_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175888
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC60800.2794261469340334No Hit
GTCCTACAGTGGACATTTCTAAATT54130.24877199561742147No Hit
CCCATGTACTCTGCGTTGATACCAC50810.23351385733089203No Hit
CTGTAGGACGTGGAATATGGCAAGA50140.23043465472487557No Hit
GTCCTAAAGTGTGTATTTCTCATTT43720.20092945960453845No Hit
CTTTAGGACGTGAAATATGGCGAGG38260.17583625627789665No Hit
GTATCAACGCAGAGTACTTTTTTTT30510.1402186141933776No Hit
GTCCTACAGTGTGCATTTCTCATTT29370.13497937393836448No Hit
GAGTACATGGGAAGCAGTGGTATCA25480.11710161552432846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC400.005269493414.25427914
TAGGACC8400.012.5501234
TCCAACG1603.6379788E-1211.87829218
GGCGAGG11150.011.6664919
CCTACAC3100.011.3355953
ATCCCGT1354.76939E-911.25357910
TGGTCGC600.005899049311.07774310
TGTAGGA26550.010.9103732
CGTGCGC700.0014981110.851665510
AGGACGT25550.010.8169895
GGACGTG25000.010.7480816
CGAAATC1701.382432E-1010.62083413
GACGTGA13200.010.5017937
TAGGACT3800.010.4971174
CCAACGA1451.456101E-810.4772319
GTCCTAA12600.010.4335651
GTAGGAC25950.010.4306593
CTGTAGG26250.010.415421
AGGACCT16050.010.4145845
GTCCTAC24800.010.3713741
Produced by FastQC (version 0.11.5)