##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512987_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2175888
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.204038535071657	31.0	30.0	34.0	25.0	34.0
2	29.327790309060024	31.0	30.0	34.0	23.0	34.0
3	29.48538941342569	31.0	30.0	34.0	25.0	34.0
4	32.98307587522887	35.0	33.0	37.0	26.0	37.0
5	33.092194083519004	35.0	35.0	37.0	27.0	37.0
6	33.20707407734221	37.0	35.0	37.0	27.0	37.0
7	33.12985732721537	37.0	35.0	37.0	26.0	37.0
8	33.13207894891649	37.0	35.0	37.0	26.0	37.0
9	34.50295833241417	39.0	35.0	39.0	27.0	39.0
10	34.3624956799247	39.0	35.0	39.0	26.0	39.0
11	34.44430733567169	39.0	35.0	39.0	27.0	39.0
12	34.330862617928865	39.0	35.0	39.0	26.0	39.0
13	34.33653570404359	39.0	35.0	39.0	26.0	39.0
14	35.37914175729633	40.0	36.0	41.0	26.0	41.0
15	35.3181308045267	40.0	36.0	41.0	25.0	41.0
16	35.208388023648276	40.0	35.0	41.0	25.0	41.0
17	35.16757204414933	40.0	35.0	41.0	25.0	41.0
18	34.856841436691596	39.0	34.0	41.0	22.0	41.0
19	35.092925279242316	40.0	35.0	41.0	24.0	41.0
20	35.183563676071564	40.0	35.0	41.0	25.0	41.0
21	35.11480554146169	40.0	35.0	41.0	25.0	41.0
22	35.04294384637445	40.0	35.0	41.0	24.0	41.0
23	34.78803366717405	39.0	35.0	41.0	19.0	41.0
24	34.7928914539719	39.0	35.0	41.0	21.0	41.0
25	34.721549087085364	39.0	35.0	41.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.41504303066780324
1101	2	0.704258159121224
1101	3	0.6623476553220797
1101	4	0.7608779334096383
1101	5	0.5349206945397498
1101	6	0.6666413026040132
1101	7	0.7498530162023087
1101	8	0.5548743411980297
1101	9	0.30519986434700286
1101	10	0.515960162673359
1101	11	0.641492397374904
1101	12	0.9022861737913956
1101	13	0.7321934377191184
1101	14	0.7344803104537618
1101	15	0.5638920699546688
1101	16	1.054304047121235
1101	17	0.9684572912203535
1101	18	1.1440205099712557
1101	19	0.7711468071340875
1101	20	0.8924031314009326
1101	21	0.8939393533120992
1101	22	0.89948825167275
1101	23	1.1310177414487015
1101	24	0.8088488431273078
1101	25	1.059666447142412
1102	1	0.43173104554866626
1102	2	0.5442704862898715
1102	3	0.6120263656178011
1102	4	0.7399907008921005
1102	5	0.559141631823806
1102	6	0.610641314372188
1102	7	0.8122381615227781
1102	8	0.8361003796204045
1102	9	0.7514985326670995
1102	10	0.8842470135341145
1102	11	0.8787514117089188
1102	12	0.8899661763803977
1102	13	0.8147477726937424
1102	14	0.8381118237277221
1102	15	1.0484867867037693
1102	16	0.9867585548306792
1102	17	0.8233876254329928
1102	18	1.0922550439163885
1102	19	1.0694922377887934
1102	20	1.0260834053935852
1102	21	0.8597235535741632
1102	22	1.127081360961995
1102	23	1.268683750936063
1102	24	1.160191353434577
1102	25	0.9778180190954586
1103	1	0.43714580182442475
1103	2	0.6640049516889555
1103	3	0.6359296918996904
1103	4	0.8301029788568925
1103	5	0.3753618166951256
1103	6	0.844189186626572
1103	7	0.5473187798693644
1103	8	0.5895270412785649
1103	9	0.9909396051580046
1103	10	0.9486872323027029
1103	11	0.8464116913344313
1103	12	0.925441663765973
1103	13	0.8995960887040013
1103	14	1.0903607080474487
1103	15	1.0745008740206927
1103	16	1.1622166442540447
1103	17	0.9860565950530571
1103	18	1.3902131198599363
1103	19	1.3621847341986708
1103	20	1.2144851832927799
1103	21	1.1296024328434484
1103	22	1.198855683510871
1103	23	1.4109717626976561
1103	24	1.2853364412960886
1103	25	1.3556595215029503
1104	1	0.38082504825810304
1104	2	0.44268736063389724
1104	3	0.4135946957068981
1104	4	0.75366272941055
1104	5	0.4035893641719994
1104	6	0.47440188511118464
1104	7	0.6852436497533674
1104	8	0.6539692631561707
1104	9	0.7290092710121243
1104	10	0.7649808736512753
1104	11	0.7935798062512021
1104	12	0.6922072424009897
1104	13	0.7469678937399706
1104	14	0.8999757877375032
1104	15	1.025384896107738
1104	16	0.8040124976180678
1104	17	0.8497607233935156
1104	18	1.053587754602951
1104	19	1.0846854355269215
1104	20	1.077303808480309
1104	21	1.0963092915724175
1104	22	0.9817472816855002
1104	23	1.311294983545487
1104	24	1.1758076119131289
1104	25	0.9387947114540083
1105	1	0.4054132380885278
1105	2	0.5596612504885066
1105	3	0.542614559753531
1105	4	0.6439025834398535
1105	5	0.3333085912070004
1105	6	0.27668349947693827
1105	7	0.5484757728993586
1105	8	0.6233371025993719
1105	9	0.4919297058820149
1105	10	0.6178105975717898
1105	11	0.5940712171802289
1105	12	0.6411570673718217
1105	13	0.6520319546223092
1105	14	0.7197351388996722
1105	15	0.8334244186470698
1105	16	0.8153630143463957
1105	17	0.6338348845107973
1105	18	0.6595338473995866
1105	19	0.8014963553739065
1105	20	0.8096013327202556
1105	21	0.8368580931587459
1105	22	0.4763795833893383
1105	23	0.6562804269013327
1105	24	0.8145306492328217
1105	25	0.7557242701776801
1106	1	0.629513166921889
1106	2	0.5828833158836879
1106	3	0.6418350387610694
1106	4	0.7383990582283531
1106	5	0.6809116851724468
1106	6	0.7348150099931203
1106	7	0.5628224750304511
1106	8	0.6922754118226706
1106	9	0.8413216472366258
1106	10	0.9244291800478166
1106	11	0.9509365899388627
1106	12	1.0025420313384004
1106	13	0.9577688520625145
1106	14	1.0822957112221303
1106	15	1.1653322960638093
1106	16	1.1140214637289105
1106	17	1.1052904376318509
1106	18	1.388632256086332
1106	19	1.0290893942913613
1106	20	1.0145694096413749
1106	21	0.8949124074027708
1106	22	0.953937123911011
1106	23	1.26903870320794
1106	24	1.1387523596440374
1106	25	1.2234526615908052
1107	1	0.5060791893204701
1107	2	0.5370226769737556
1107	3	0.7527311858407302
1107	4	0.7946098366874068
1107	5	0.7001962967458084
1107	6	0.5520031190515198
1107	7	0.7757221473427336
1107	8	0.6805787449022347
1107	9	0.8482132814860464
1107	10	0.7496716593920851
1107	11	0.6770382459329056
1107	12	0.7636562278253933
1107	13	0.7072168332490705
1107	14	1.0535074485744147
1107	15	1.1686366296371773
1107	16	1.1334259681558336
1107	17	1.0804497366288999
1107	18	1.3477372280621083
1107	19	1.3390592629957894
1107	20	1.0630059560782215
1107	21	1.031263695959744
1107	22	1.0147015843744498
1107	23	1.0223259181452065
1107	24	1.1864659713101702
1107	25	1.2498629522415357
1108	1	0.6698604019648968
1108	2	0.6868900576288794
1108	3	0.5888487897610908
1108	4	0.7943678512826438
1108	5	0.7495054259493301
1108	6	0.8417603388101895
1108	7	0.9174623857098609
1108	8	0.8420951253989699
1108	9	1.005052866615877
1108	10	0.9009983501274235
1108	11	0.8502771096108717
1108	12	0.9378212774887089
1108	13	0.9348745485106633
1108	14	1.1890388379199663
1108	15	1.1896869761637276
1108	16	1.1214436630097708
1108	17	1.2749475606096752
1108	18	1.3636383158703396
1108	19	1.304365098764798
1108	20	1.239988853447393
1108	21	1.1560746551367913
1108	22	1.0969924858465276
1108	23	1.3073511067380608
1108	24	1.3675680455048393
1108	25	1.3437395625356316
1109	1	0.1638157973426111
1109	2	-0.004039720804936309
1109	3	0.2420381344392908
1109	4	0.25247580849338647
1109	5	0.16711840136282063
1109	6	0.05155062968371027
1109	7	0.25293699700930716
1109	8	0.29488605878191265
1109	9	0.402208634442367
1109	10	0.5090849953838301
1109	11	0.11849996484812664
1109	12	0.382829503575401
1109	13	0.3738703080493124
1109	14	0.12184782911968028
1109	15	0.4354227578385732
1109	16	0.4402760451821379
1109	17	0.17340964663031855
1109	18	-1.3560859167669719
1109	19	-0.004392816338757655
1109	20	0.28665459756228273
1109	21	0.4099525864127358
1109	22	0.6319636258558532
1109	23	0.10304294117177903
1109	24	0.47712787487812847
1109	25	0.5592285011146032
1110	1	0.22807191208904243
1110	2	0.2445853977478052
1110	3	0.3087691077885246
1110	4	0.2115413693764907
1110	5	0.3945002071286865
1110	6	0.3242466238229227
1110	7	0.38084537726610534
1110	8	0.30278310913636375
1110	9	0.6796606295292378
1110	10	0.818942662638591
1110	11	0.6443749766112887
1110	12	0.07956589708350492
1110	13	0.3326984458143514
1110	14	0.40578521074164087
1110	15	0.6183796743094305
1110	16	0.6616011473956291
1110	17	0.25672542522186603
1110	18	0.7964892262099426
1110	19	0.6740235418176184
1110	20	0.6067007913088673
1110	21	0.6038194466770719
1110	22	0.6765043891521216
1110	23	0.6526486604007715
1110	24	0.7751292939958319
1110	25	0.7127961963917286
1111	1	0.10494709867023033
1111	2	0.3078647164982762
1111	3	0.34953162617818023
1111	4	0.2315616006834702
1111	5	0.2831125585348886
1111	6	0.4302017154758957
1111	7	0.2876550805617484
1111	8	0.2936624407730122
1111	9	0.5668270653184635
1111	10	0.45609859618884485
1111	11	0.26644686003428575
1111	12	0.5725035386972834
1111	13	0.44060630960739644
1111	14	0.41264667353816264
1111	15	0.6555836303675449
1111	16	0.38034654863914596
1111	17	0.46584162637518034
1111	18	0.6914910624909893
1111	19	0.5064084950515095
1111	20	0.31138066631289263
1111	21	0.5962198633303188
1111	22	0.2657663330327935
1111	23	0.5863668610601138
1111	24	0.6656551576385397
1111	25	0.10991295594629946
1112	1	-0.15291696556908718
1112	2	0.0915813891090238
1112	3	0.19858928377741947
1112	4	0.21076258632040634
1112	5	0.12421206247419292
1112	6	0.06643372922294333
1112	7	-0.19775295367616508
1112	8	0.0215694969391933
1112	9	0.25935805560258984
1112	10	0.29711215950413106
1112	11	0.13945316076162584
1112	12	0.33562714318885156
1112	13	0.08797316756544404
1112	14	0.1641723573778222
1112	15	0.22797593980566688
1112	16	0.25569434230383337
1112	17	0.34430844218427836
1112	18	0.3235276348088618
1112	19	0.30802926259465835
1112	20	0.028448915186217505
1112	21	0.049855026360283716
1112	22	0.05896284439680244
1112	23	0.33309793996510706
1112	24	-0.012836126175180596
1112	25	0.18072543635784655
1113	1	-0.25701963168588904
1113	2	-0.11030987896574018
1113	3	-0.3250846934349312
1113	4	-0.08471522175248936
1113	5	-0.2616429063441501
1113	6	-0.15742379053141775
1113	7	-0.3157103133953072
1113	8	-0.07361050632155752
1113	9	-0.3121888786348137
1113	10	-0.4722212760004538
1113	11	-0.36019044471435535
1113	12	-0.0708356022507175
1113	13	-0.2654590422498515
1113	14	-0.22088247788082072
1113	15	-0.4287102910677376
1113	16	-0.5037994405444408
1113	17	-0.5216237708203266
1113	18	-0.5478459626717367
1113	19	-0.528338916889723
1113	20	-0.6660535770240017
1113	21	-0.3880971590257616
1113	22	-0.2874031348903898
1113	23	-0.6845417391752093
1113	24	-0.21573521553848707
1113	25	-0.2240008797780888
1114	1	-1.4594605790805133
1114	2	-1.3070041669837487
1114	3	-1.077801166468479
1114	4	-1.3380280181324267
1114	5	-1.430405689640292
1114	6	-1.8104925262110427
1114	7	-1.9303497856183007
1114	8	-1.5202461831794025
1114	9	-1.687360552956747
1114	10	-1.7007589328730646
1114	11	-2.3375866625393797
1114	12	-1.78545699117565
1114	13	-1.7780668452993353
1114	14	-2.1102622207575976
1114	15	-2.125879467767085
1114	16	-2.6292949760972704
1114	17	-4.589120255214439
1114	18	-3.7248398377523166
1114	19	-2.602689098892796
1114	20	-1.8061732671826505
1114	21	-2.8128290688135067
1114	22	-2.7745580789704505
1114	23	-2.812227353592494
1114	24	-2.231646815867812
1114	25	-2.0564109153702717
1115	1	-1.08268467486349
1115	2	-0.6248061942234351
1115	3	-0.7597877061488312
1115	4	-0.6923562309106757
1115	5	-0.5967267799175602
1115	6	-0.7257058778823762
1115	7	-1.8037644902199013
1115	8	-1.1763730430273824
1115	9	-0.8568499489669392
1115	10	-0.6738045781018087
1115	11	-1.506340871583923
1115	12	-1.0685724386385829
1115	13	-0.7877900938841051
1115	14	-0.9026433004287213
1115	15	-2.131023707034835
1115	16	-0.8985621604243192
1115	17	-0.9469976464475565
1115	18	-0.9977958050026103
1115	19	-1.0522226440616151
1115	20	-1.0165628921303735
1115	21	-1.795598911144097
1115	22	-1.825587608031718
1115	23	-1.2616014616630835
1115	24	-1.1497958279010447
1115	25	-1.5510926663328064
1116	1	-1.3937888528894185
1116	2	-2.1056466815417494
1116	3	-1.67558722779399
1116	4	-1.4165434659325626
1116	5	-1.775598919676444
1116	6	-1.5992336464026415
1116	7	-1.312804427650537
1116	8	-1.9983149846729376
1116	9	-2.109063521936406
1116	10	-2.9530283593055273
1116	11	-1.6961553320182077
1116	12	-1.6931530538109172
1116	13	-2.111441286835529
1116	14	-1.9618845879996556
1116	15	-1.6512321549421145
1116	16	-1.5344755659847351
1116	17	-1.8628353570736635
1116	18	-1.6366942598293335
1116	19	-2.037218159800439
1116	20	-2.353664176878347
1116	21	-2.719980607148706
1116	22	-1.9877518162757255
1116	23	-1.9910138577390057
1116	24	-2.5218313661966008
1116	25	-2.271833892582663
1201	1	0.3631173265904586
1201	2	0.3174832172105262
1201	3	0.5439781183434249
1201	4	0.6994683848715013
1201	5	0.6583144816118107
1201	6	0.6211557753049561
1201	7	0.6104996318438012
1201	8	0.13362602695535486
1201	9	0.5585449250050729
1201	10	0.9464299708327175
1201	11	0.37513854450722306
1201	12	-0.15214371913747016
1201	13	0.28155529465013274
1201	14	0.8623537604327467
1201	15	0.6086066004530153
1201	16	0.9161881618870069
1201	17	0.9395104297939838
1201	18	1.380588818901245
1201	19	0.8711560021740183
1201	20	0.970679010329988
1201	21	0.9357309922476986
1201	22	1.0759239185028804
1201	23	1.2468338304333315
1201	24	1.0309988186072019
1201	25	0.9606220015303464
1202	1	0.6763744661574798
1202	2	0.8429988925905363
1202	3	0.4555586831752976
1202	4	0.9544930710760156
1202	5	0.8636993848821959
1202	6	0.8882749024353132
1202	7	0.8910882142942853
1202	8	0.9937959515292789
1202	9	1.2200650338258967
1202	10	1.2884836039511
1202	11	0.9923062523607413
1202	12	1.0631653262333245
1202	13	1.0847886116699357
1202	14	1.338776635835785
1202	15	0.9550620069477205
1202	16	1.234932044314462
1202	17	1.4349647135341925
1202	18	1.8115070305548642
1202	19	1.4859778008842923
1202	20	1.3743254434253345
1202	21	1.384691364771875
1202	22	1.4749213563195553
1202	23	1.5095493849013195
1202	24	1.6648412299383821
1202	25	1.5413984294437455
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2211	12	0.18147638393231347
2211	13	0.011541602208581025
2211	14	0.2343154885248424
2211	15	0.2262430786789622
2211	16	0.14386962159609595
2211	17	0.18143997646540555
2211	18	0.1760262519728144
2211	19	0.08056669733732491
2211	20	-0.10971807047494053
2211	21	0.07183204860143633
2211	22	-0.2020591374492895
2211	23	0.3813174496998002
2211	24	0.1990198564440746
2211	25	0.33734261994904813
2212	1	-0.09325389888893199
2212	2	-0.09798904557325372
2212	3	-0.2059738391725574
2212	4	-0.22028430826272682
2212	5	-0.09919116639347436
2212	6	-0.1967807741770784
2212	7	-0.25261235019279127
2212	8	-0.19615566386457317
2212	9	-0.11797607593166504
2212	10	-0.05924386374960022
2212	11	-0.31896118411301444
2212	12	-0.1931795770832494
2212	13	-0.190424572277351
2212	14	-0.18208536509072815
2212	15	-0.3909136634879147
2212	16	-0.2579775519657588
2212	17	-0.21291545735611805
2212	18	-3.000184200164213
2212	19	-1.3111319909932746
2212	20	-0.6085709414553335
2212	21	-0.63601021201724
2212	22	-0.38831835337281007
2212	23	-0.4062193641335625
2212	24	-0.8654326063432052
2212	25	-0.5653925730784266
2213	1	-0.282166180885671
2213	2	-0.1877966591631015
2213	3	-0.5266756771438779
2213	4	-0.548266398275679
2213	5	-0.5164822892402867
2213	6	-0.4742859243887878
2213	7	-0.41754007946947524
2213	8	-0.8248745528951247
2213	9	-0.6820388380556324
2213	10	-0.7555508096572794
2213	11	-0.6865824573392914
2213	12	-0.8303337412055711
2213	13	-0.691455621175848
2213	14	-0.5917296817398352
2213	15	-0.81454491503969
2213	16	-1.287627169809987
2213	17	-0.8036608456518834
2213	18	-0.706367641646402
2213	19	-0.8054535051219673
2213	20	-1.3048928179442
2213	21	-1.0143462742358196
2213	22	-0.7969945308756081
2213	23	-1.212734969107288
2213	24	-1.0559703085770877
2213	25	-1.238201971627646
2214	1	-1.7964919130743198
2214	2	-3.7226197180275484
2214	3	-2.0104579141838492
2214	4	-1.7166115243587967
2214	5	-1.8439525662368688
2214	6	-1.8373416672251217
2214	7	-1.8916551243479702
2214	8	-1.5782992372775233
2214	9	-1.7290905199478743
2214	10	-1.9055502395001582
2214	11	-1.647134889700844
2214	12	-2.157762325462677
2214	13	-1.9237999932261758
2214	14	-3.1986271931465424
2214	15	-3.3305485127311236
2214	16	-2.57106563774078
2214	17	-3.0485786076141324
2214	18	-2.4603831513454892
2214	19	-4.4790469275043385
2214	20	-2.8003577525685586
2214	21	-2.642280801668221
2214	22	-2.801519796675734
2214	23	-2.8277689789795595
2214	24	-3.1537600944973434
2214	25	-2.8897360149966467
2215	1	-0.8862041166590799
2215	2	-2.4743166099751264
2215	3	-1.352458941415236
2215	4	-0.9790939845544813
2215	5	-0.9412103859590246
2215	6	-1.391773549192152
2215	7	-1.8994983774376593
2215	8	-1.9311887184895404
2215	9	-1.4586074520127568
2215	10	-2.9980930201826475
2215	11	-2.0237942270011047
2215	12	-2.2496152568569485
2215	13	-1.5577449321062673
2215	14	-2.9466066492414313
2215	15	-2.76124589372467
2215	16	-2.220578750658369
2215	17	-2.675520993591178
2215	18	-1.5079057911747853
2215	19	-2.0449245084774006
2215	20	-2.1522718517438975
2215	21	-1.9905420508545717
2215	22	-1.5075155508100835
2215	23	-2.084365634569977
2215	24	-1.885312158742849
2215	25	-1.831909807155185
2216	1	-1.5888627135116202
2216	2	-1.7453457451482066
2216	3	-2.125240973435652
2216	4	-3.030943401949493
2216	5	-2.019427725825299
2216	6	-2.0154818021649668
2216	7	-2.080950764619928
2216	8	-2.499706262053035
2216	9	-2.8709845021731972
2216	10	-2.185431244077961
2216	11	-2.6117214741767008
2216	12	-3.1928638476464037
2216	13	-3.8125806308875987
2216	14	-3.123129173398823
2216	15	-3.1145438051248178
2216	16	-2.2468988436725965
2216	17	-2.371428779101386
2216	18	-2.378573708441081
2216	19	-2.810414130891971
2216	20	-2.6440377117808396
2216	21	-2.975077486151868
2216	22	-3.441372950800801
2216	23	-3.3091851838758153
2216	24	-3.3309281318120476
2216	25	-3.45046417236685
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89693.0
3	3.0
4	715.0
5	4349.0
6	5482.0
7	5000.0
8	4611.0
9	4210.0
10	4142.0
11	3810.0
12	3613.0
13	3648.0
14	3688.0
15	3679.0
16	3840.0
17	4149.0
18	4692.0
19	5572.0
20	7252.0
21	9198.0
22	11559.0
23	14044.0
24	15989.0
25	18583.0
26	21122.0
27	23487.0
28	26942.0
29	31702.0
30	39435.0
31	49488.0
32	62479.0
33	80936.0
34	105408.0
35	140700.0
36	195474.0
37	312890.0
38	854304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.211326986235655	18.66734012325178	12.665688770803351	25.455644119709213
2	14.473862072072983	19.871197749661835	41.90100792565571	23.753932252609474
3	19.05376446913443	24.42910796524787	29.344924119369526	27.172203446248172
4	12.249818281626927	16.720573002511642	38.18678855705267	32.842820158808756
5	12.937024353820068	38.17735326464779	36.04087660510471	12.844745776427443
6	32.14165052091593	36.83331839420454	18.1350451276606	12.889985957218933
7	28.145975121052963	31.37586221796061	22.09870493782102	18.379457723165405
8	24.86483827711957	37.53370136487942	20.48417799928931	17.117282358711698
9	26.474964662077525	15.093740303618834	20.194812507900757	38.23648252640289
10	14.955712468678794	28.995335662667326	34.75898931849743	21.289962550156453
11	34.03951153454018	22.28681119355577	24.978648252834105	18.695029019069935
12	23.7067249658231	24.497321468793064	31.788102301780874	20.00785126360296
13	29.048250335793213	21.931594716669593	25.59755562767359	23.422599319863604
14	20.553777239609264	22.68426257045648	27.432067334810846	29.32989285512341
15	23.020377878231063	31.47889599866866	22.96994691647079	22.530779206629486
16	21.68584922226298	28.124163952240817	28.611473494240634	21.578513331255568
17	21.64404694702738	28.9746013125015	28.101423746016803	21.279927994454322
18	21.57133997777009	27.335520746689433	30.316316165266873	20.776823110273604
19	23.422496570644718	27.136681413813772	27.546227341431397	21.894594674110117
20	23.586220972486352	27.596944552087834	28.045231367389707	20.77160310803611
21	22.55020537255524	27.013926718659135	27.2888754042518	23.146992504533827
22	22.964150290308684	28.581396984430295	26.849933553763535	21.604519171497483
23	21.71717937862262	28.364300151742693	27.826742110506007	22.09177835912867
24	22.957146573923147	27.292639448540122	28.371306876424413	21.378907101112315
25	22.40673056016137	27.497677429023103	27.93847175059564	22.157120260219887
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	206.0
1	206.0
2	370.0
3	534.0
4	534.0
5	534.0
6	1014.0
7	1494.0
8	1494.0
9	1494.0
10	2300.5
11	3107.0
12	3107.0
13	3107.0
14	5742.0
15	8377.0
16	8377.0
17	8377.0
18	15810.5
19	23244.0
20	23244.0
21	23244.0
22	38139.5
23	53035.0
24	53035.0
25	53035.0
26	77748.5
27	102462.0
28	102462.0
29	102462.0
30	135220.0
31	167978.0
32	167978.0
33	167978.0
34	189990.5
35	212003.0
36	212003.0
37	212003.0
38	228908.0
39	245813.0
40	245813.0
41	245813.0
42	257366.0
43	268919.0
44	268919.0
45	268919.0
46	284190.5
47	299462.0
48	299462.0
49	299462.0
50	283582.0
51	267702.0
52	267702.0
53	267702.0
54	235074.5
55	202447.0
56	202447.0
57	202447.0
58	173029.0
59	143611.0
60	143611.0
61	143611.0
62	116532.5
63	89454.0
64	89454.0
65	89454.0
66	69146.0
67	48838.0
68	48838.0
69	48838.0
70	35931.0
71	23024.0
72	23024.0
73	23024.0
74	16061.5
75	9099.0
76	9099.0
77	9099.0
78	6273.0
79	3447.0
80	3447.0
81	3447.0
82	2309.5
83	1172.0
84	1172.0
85	1172.0
86	765.5
87	359.0
88	359.0
89	359.0
90	217.5
91	76.0
92	76.0
93	76.0
94	45.0
95	14.0
96	14.0
97	14.0
98	12.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.36619531887670687
2	0.042557337510018896
3	0.03800747097277066
4	0.03763980498996272
5	0.0437522519541447
6	0.018061591405439986
7	0.018107549653290977
8	0.024403829608876926
9	0.021278668755009448
10	0.020451420293691586
11	0.019394380593118765
12	0.02031354555013861
13	0.02031354555013861
14	0.027988572941254328
15	0.029275403881082115
16	0.021784209481370366
17	0.022979123925496166
18	0.10294647518622282
19	0.025368952813747767
20	0.022749332686241203
21	0.026334076018618605
22	0.020451420293691586
23	0.022749332686241203
24	0.019578213584522733
25	0.022841249181943187
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2175888.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.98712879544134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.82276483242569	51.224651701776324
2	13.56502359243939	17.631038706301183
3	4.0120924589437275	7.822031081057849
4	1.562176149142774	4.060853704220321
5	0.7243770425129944	2.353759207912643
6	0.37005225985979384	1.4429180323531532
7	0.23259162479561823	1.0580823314133667
8	0.1444664143765916	0.7510765982165726
9	0.09523615109662777	0.5570211615568794
>10	0.4205225619640739	4.665088657305996
>50	0.026819304314192945	1.2133086539234785
>100	0.01877915697582864	2.631750617996526
>500	0.0028324728626177745	1.3176036871674595
>1k	0.0019827310038323426	2.277366976478793
>5k	2.8324728626176324E-4	0.9934488823194347
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	6080	0.2794261469340334	No Hit
GTCCTACAGTGGACATTTCTAAATT	5413	0.24877199561742147	No Hit
CCCATGTACTCTGCGTTGATACCAC	5081	0.23351385733089203	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5014	0.23043465472487557	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4372	0.20092945960453845	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3826	0.17583625627789665	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3051	0.1402186141933776	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2937	0.13497937393836448	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2548	0.11710161552432846	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	4.595824785099233E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCC	40	0.0052694934	14.254279	14
TAGGACC	840	0.0	12.550123	4
TCCAACG	160	3.6379788E-12	11.878292	18
GGCGAGG	1115	0.0	11.66649	19
CCTACAC	310	0.0	11.335595	3
ATCCCGT	135	4.76939E-9	11.253579	10
TGGTCGC	60	0.0058990493	11.077743	10
TGTAGGA	2655	0.0	10.910373	2
CGTGCGC	70	0.00149811	10.8516655	10
AGGACGT	2555	0.0	10.816989	5
GGACGTG	2500	0.0	10.748081	6
CGAAATC	170	1.382432E-10	10.620834	13
GACGTGA	1320	0.0	10.501793	7
TAGGACT	380	0.0	10.497117	4
CCAACGA	145	1.456101E-8	10.47723	19
GTCCTAA	1260	0.0	10.433565	1
GTAGGAC	2595	0.0	10.430659	3
CTGTAGG	2625	0.0	10.41542	1
AGGACCT	1605	0.0	10.414584	5
GTCCTAC	2480	0.0	10.371374	1
>>END_MODULE