FastQCFastQC Report
Thu 26 May 2016
SRR1512987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512987_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175888
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC58540.2690395829197091No Hit
CCCATGTACTCTGCGTTGATACCAC58500.26885574992830513No Hit
GTCCTACAGTGGACATTTCTAAATT50560.23236490113461725No Hit
CTGTAGGACGTGGAATATGGCAAGA50040.22997507224636562No Hit
GTCCTAAAGTGTGTATTTCTCATTT42240.1941276389225916No Hit
CTTTAGGACGTGAAATATGGCGAGG41790.19205951776929694No Hit
GTATCAACGCAGAGTACTTTTTTTT36880.1694940180744597No Hit
GTCCTACAGTGTGCATTTCTCATTT28540.1311648393667321No Hit
TATCAACGCAGAGTACTTTTTTTTT25050.11512541086673579No Hit
GAGTACATGGGAAGCAGTGGTATCA22340.10267072569911688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG400.005252831614.2610851
GGCGAGG11450.012.68171819
TAGGACC8750.012.2863174
CTAGTCG658.0772553E-411.68338214
TTAGGAC15500.011.1001333
GTCCTAG3100.011.040841
CTAACAC2000.010.9314843
GTCTAGG1855.456968E-1210.7921721
GTACTAA1855.456968E-1210.7921721
GTCCTAC23950.010.6784461
GACGTGA14650.010.63997
TGTAGGA28100.010.5204762
CAGGACT2900.010.497924
CGTGCGC1002.394233E-510.45211210
TGGCGAG26300.010.42879818
AGGACCT16100.010.4001145
GGACCTG15800.010.353636
AGGACGT27000.010.3241715
GGACGTG26300.010.2703176
TCCAACG1303.8956932E-710.22061118
Produced by FastQC (version 0.11.5)