##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512987_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2175888 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.532892777569433 31.0 31.0 34.0 27.0 34.0 2 30.69597791798107 31.0 31.0 34.0 27.0 34.0 3 30.801381780679886 31.0 31.0 34.0 27.0 34.0 4 34.25887178016516 37.0 35.0 37.0 30.0 37.0 5 33.92413304361254 37.0 35.0 37.0 30.0 37.0 6 33.785396582912355 37.0 35.0 37.0 29.0 37.0 7 33.66323726221203 37.0 35.0 37.0 28.0 37.0 8 33.54974612663887 37.0 35.0 37.0 28.0 37.0 9 35.02330772539763 39.0 35.0 39.0 28.0 39.0 10 34.91500113976455 39.0 35.0 39.0 27.0 39.0 11 34.93622098196231 39.0 35.0 39.0 27.0 39.0 12 34.78984855838168 39.0 35.0 39.0 27.0 39.0 13 34.772872500790484 39.0 35.0 39.0 27.0 39.0 14 35.83667403836962 40.0 36.0 41.0 27.0 41.0 15 35.85156818733317 40.0 36.0 41.0 27.0 41.0 16 35.830270216114066 40.0 36.0 41.0 27.0 41.0 17 35.81022047090659 40.0 36.0 41.0 27.0 41.0 18 35.73712433728207 40.0 36.0 41.0 27.0 41.0 19 35.71661776709095 40.0 36.0 41.0 27.0 41.0 20 35.69617553844683 40.0 36.0 41.0 27.0 41.0 21 35.64487556344812 40.0 36.0 41.0 27.0 41.0 22 35.605977421632 40.0 36.0 41.0 27.0 41.0 23 35.498767399792634 40.0 36.0 41.0 27.0 41.0 24 35.485327829373574 40.0 36.0 41.0 27.0 41.0 25 35.39149625348364 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5455258263650542 1101 2 0.5725241511696595 1101 3 0.5890702061242514 1101 4 0.29099186044815895 1101 5 0.5442268171427003 1101 6 0.5312760641430359 1101 7 0.5880029812786702 1101 8 0.5559751151456069 1101 9 0.32142552292803117 1101 10 0.616161115897988 1101 11 0.6846130716908903 1101 12 0.7625767754722972 1101 13 0.7633967220794062 1101 14 0.8190229565151625 1101 15 0.7916924876540321 1101 16 0.9569176543705566 1101 17 0.8729559438219923 1101 18 0.26228877691725216 1101 19 0.7446510271972429 1101 20 0.6585617914746607 1101 21 0.880646611870695 1101 22 0.7705174473991434 1101 23 0.7840835203087693 1101 24 0.7103930702807588 1101 25 0.8386947587785656 1102 1 0.40393972576255877 1102 2 0.4156366582811337 1102 3 0.4261241231446036 1102 4 0.36723789926035266 1102 5 0.4962021613343879 1102 6 0.42818129020793805 1102 7 0.36394749725158704 1102 8 0.4176445712387107 1102 9 0.6419574667335013 1102 10 0.6627877486870091 1102 11 0.7407005857621414 1102 12 0.712841504361009 1102 13 0.8399617888987692 1102 14 0.9210343223705948 1102 15 0.8086480447541646 1102 16 0.7733340449446899 1102 17 0.7111451224655383 1102 18 0.7450419573598595 1102 19 0.7611459703734766 1102 20 0.733175546932614 1102 21 0.8282592710491272 1102 22 0.8084205489397789 1102 23 0.8370872195626262 1102 24 0.8217404643827706 1102 25 0.8500078412804939 1103 1 0.6740378768696118 1103 2 0.4949562685524036 1103 3 0.5543652574629832 1103 4 0.617627466234417 1103 5 0.6104032479880956 1103 6 0.741074294537654 1103 7 0.7033155989505815 1103 8 0.8354140374555712 1103 9 0.9577063968846389 1103 10 0.9285144820578424 1103 11 0.92004607729951 1103 12 0.9450261168699328 1103 13 0.9605093574077372 1103 14 1.09498572669888 1103 15 0.8484814700498617 1103 16 1.024919362358645 1103 17 1.1642486277595196 1103 18 1.254717417066864 1103 19 1.2112474285312018 1103 20 1.1633270422047843 1103 21 0.7771337055704208 1103 22 1.043805991087062 1103 23 1.096597776063767 1103 24 1.1558994220866694 1103 25 1.1527541311830234 1104 1 0.3663589858917433 1104 2 0.43876166695041263 1104 3 0.3354966524005256 1104 4 0.39307696972886674 1104 5 0.5048598797654975 1104 6 0.5157548634760474 1104 7 0.5986791657754011 1104 8 0.42187517864297064 1104 9 0.6430786932859931 1104 10 0.7211100238753048 1104 11 0.6563727899779579 1104 12 0.5649366953394122 1104 13 0.5867818979236006 1104 14 0.7426229038918564 1104 15 0.7549401369860291 1104 16 0.758825142174679 1104 17 0.6714906914244381 1104 18 0.7313768642633818 1104 19 0.7042893810290636 1104 20 0.7719630502269368 1104 21 0.6640400377369033 1104 22 0.7233021150505365 1104 23 0.613577063793052 1104 24 0.6917105495959888 1104 25 0.8240092561065779 1105 1 0.46131787853160944 1105 2 0.5187867239744968 1105 3 0.4770359498583048 1105 4 0.40740202312149876 1105 5 0.4471084753182737 1105 6 0.5462943534693352 1105 7 0.5837227192273033 1105 8 0.6814550195579301 1105 9 0.7707811279352583 1105 10 0.6693551414555756 1105 11 0.7186318208185796 1105 12 0.60948948193127 1105 13 0.8418532559704559 1105 14 0.9122538866246401 1105 15 0.7456120996355082 1105 16 0.823097916351081 1105 17 0.7324180753875353 1105 18 0.8859335495281115 1105 19 0.8782013669472448 1105 20 0.9173205119373762 1105 21 0.8413481171711652 1105 22 0.9319420948788419 1105 23 0.9109228144109096 1105 24 0.9711011644146481 1105 25 1.0485721285446061 1106 1 0.5268598914774856 1106 2 0.500035333337582 1106 3 0.4400977459412516 1106 4 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0.8301106717874518 1107 17 0.8295734386635232 1107 18 0.9517163052612858 1107 19 1.1277459109293062 1107 20 1.0360195367962888 1107 21 0.9012055260057608 1107 22 0.8722961150139525 1107 23 0.8218705330956624 1107 24 0.889271483455623 1107 25 0.9083910533049036 1108 1 0.5927408584808624 1108 2 0.5467196581520533 1108 3 0.5316292448869113 1108 4 0.5515932729046185 1108 5 0.61272456424269 1108 6 0.6675046136588989 1108 7 0.6179457799876502 1108 8 0.6826488121385452 1108 9 0.6442453228832932 1108 10 0.8216863132264649 1108 11 0.8140988991922526 1108 12 0.9280450910700395 1108 13 0.8005935392589691 1108 14 0.9441308081113675 1108 15 0.9493954883251092 1108 16 0.8856876668334124 1108 17 0.9378905937437025 1108 18 0.8792478412447409 1108 19 0.9540748600033027 1108 20 1.0718331301126938 1108 21 1.1483742186837205 1108 22 1.0008724788758343 1108 23 0.8786094526259021 1108 24 1.0824251541333823 1108 25 1.0709032399085316 1109 1 0.0704041406932312 1109 2 0.025070064994867636 1109 3 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2216 14 -1.9937360236210608 2216 15 -2.051725963817404 2216 16 -1.8438759039301829 2216 17 -1.9763681539919276 2216 18 -1.6978644314149989 2216 19 -1.9802365322602853 2216 20 -2.334598794913923 2216 21 -2.2274178856934483 2216 22 -2.384740424877606 2216 23 -1.5533907974943872 2216 24 -1.7246311515558688 2216 25 -1.691771196843753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52606.0 3 2.0 4 879.0 5 7525.0 6 9149.0 7 7044.0 8 5574.0 9 5049.0 10 4430.0 11 3989.0 12 3652.0 13 3412.0 14 3364.0 15 3432.0 16 3458.0 17 3683.0 18 4083.0 19 4845.0 20 6032.0 21 7859.0 22 10033.0 23 12126.0 24 14543.0 25 16991.0 26 19394.0 27 21241.0 28 24185.0 29 29102.0 30 36488.0 31 46804.0 32 59890.0 33 77194.0 34 100734.0 35 133018.0 36 183678.0 37 285957.0 38 964443.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.709898819670144 19.294405414927663 13.688597503930295 27.3070982614719 2 13.335579161775938 20.12724931361294 40.818958346163356 25.71821317844777 3 17.243626510188022 24.420926077077496 30.012160552381374 28.323286860353107 4 11.33497171899939 17.122279470715004 37.548176997138796 33.9945718131468 5 12.491491811903737 38.268220399783665 36.23177408139163 13.008513706920974 6 32.23651227461431 37.18683336384318 17.755576631364175 12.82107773017834 7 27.81834422980467 31.47474977806322 22.137314387985484 18.56959160414662 8 24.850131703835597 37.75696963454025 20.233553575501613 17.159345086122535 9 26.08170916231534 14.937285259311809 20.0760273415212 38.90497823685165 10 14.603298891858307 29.17816619963113 34.78374283862224 21.43479206988833 11 33.90312303523434 22.266516900201854 24.915710404112083 18.914649660451726 12 23.430885034294693 24.588213178189466 31.822387359292474 20.158514428223366 13 29.03031887929208 21.784148551432345 25.591231704183997 23.59430086509158 14 20.359508485139287 22.59367112111201 27.361701697395535 29.685118696353165 15 22.903908977430035 31.497758078363386 22.983833162056282 22.614499782150297 16 21.585256548738055 28.045789456750057 28.578559576394667 21.790394418117216 17 21.48781263675847 28.99716277889534 28.117817307232617 21.397207277113573 18 21.556470948875187 27.373038199415316 30.19489666437027 20.87559418733923 19 23.402121649706274 27.03407489656935 27.618213354388665 21.94559009933571 20 23.477848034362918 27.510646492333514 28.005858902839094 21.005646570464474 21 22.399805136760445 26.930651206290772 27.358660958644414 23.31088269830437 22 22.92409373490554 28.438090134524817 26.888970490573705 21.74884563999594 23 21.55382078489334 28.25765848242189 27.85897068231453 22.32955005037024 24 22.995714852970373 27.18002948681182 28.33174317795769 21.492512482260118 25 22.290338547686815 27.42335994205491 27.93998491626043 22.346316593997848 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 102.0 1 102.0 2 247.5 3 393.0 4 393.0 5 393.0 6 961.0 7 1529.0 8 1529.0 9 1529.0 10 2174.5 11 2820.0 12 2820.0 13 2820.0 14 5453.5 15 8087.0 16 8087.0 17 8087.0 18 15542.0 19 22997.0 20 22997.0 21 22997.0 22 38071.5 23 53146.0 24 53146.0 25 53146.0 26 78211.0 27 103276.0 28 103276.0 29 103276.0 30 135739.0 31 168202.0 32 168202.0 33 168202.0 34 189856.0 35 211510.0 36 211510.0 37 211510.0 38 227890.5 39 244271.0 40 244271.0 41 244271.0 42 257063.5 43 269856.0 44 269856.0 45 269856.0 46 285270.5 47 300685.0 48 300685.0 49 300685.0 50 285407.0 51 270129.0 52 270129.0 53 270129.0 54 236347.0 55 202565.0 56 202565.0 57 202565.0 58 172951.0 59 143337.0 60 143337.0 61 143337.0 62 116108.0 63 88879.0 64 88879.0 65 88879.0 66 68596.0 67 48313.0 68 48313.0 69 48313.0 70 35368.0 71 22423.0 72 22423.0 73 22423.0 74 15570.5 75 8718.0 76 8718.0 77 8718.0 78 5944.0 79 3170.0 80 3170.0 81 3170.0 82 2114.5 83 1059.0 84 1059.0 85 1059.0 86 702.5 87 346.0 88 346.0 89 346.0 90 207.5 91 69.0 92 69.0 93 69.0 94 37.5 95 6.0 96 6.0 97 6.0 98 3.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08042693373923658 2 9.191649570198466E-5 3 0.0 4 0.026471950762171584 5 0.0694888707507004 6 0.07932393579081276 7 0.03304398020486348 8 0.06029722118050194 9 0.04048921635672424 10 0.10336009941688176 11 0.0025736618796555707 12 0.008594192348135566 13 0.002251954144698624 14 0.003860492819483356 15 0.003492826836675417 16 0.012776392902575867 17 0.023898288882516012 18 7.812902134668695E-4 19 0.001562580426933739 20 0.0024817453839535856 21 1.37874743552977E-4 22 3.2170773495694634E-4 23 0.0 24 0.0 25 0.0016085386747847316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2175888.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.899696437839204 #Duplication Level Percentage of deduplicated Percentage of total 1 77.97166028889916 49.82365423214978 2 13.989610202755825 17.878636904795908 3 4.237668653734075 8.123572217332626 4 1.6467201957852038 4.208996825149347 5 0.7680000885220252 2.4537486260395527 6 0.40128009108826607 1.53850056042532 7 0.2245733999209865 1.0045120458075147 8 0.14325932452895426 0.7323381879432055 9 0.1077729272600723 0.6197991602522351 >10 0.4587781961650107 4.988267225142602 >50 0.02578357679331849 1.1624026186110037 >100 0.019572436434094852 2.678202621572668 >500 0.0028041095460041203 1.2485292366085226 >1k 0.00222890758784913 2.5389134919674925 >5k 2.8760097907730713E-4 0.9999260462022134 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 5854 0.2690395829197091 No Hit CCCATGTACTCTGCGTTGATACCAC 5850 0.26885574992830513 No Hit GTCCTACAGTGGACATTTCTAAATT 5056 0.23236490113461725 No Hit CTGTAGGACGTGGAATATGGCAAGA 5004 0.22997507224636562 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4224 0.1941276389225916 No Hit CTTTAGGACGTGAAATATGGCGAGG 4179 0.19205951776929694 No Hit GTATCAACGCAGAGTACTTTTTTTT 3688 0.1694940180744597 No Hit GTCCTACAGTGTGCATTTCTCATTT 2854 0.1311648393667321 No Hit TATCAACGCAGAGTACTTTTTTTTT 2505 0.11512541086673579 No Hit GAGTACATGGGAAGCAGTGGTATCA 2234 0.10267072569911688 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.595824785099233E-5 0.0 5 0.0 0.0 0.0 4.595824785099233E-5 0.0 6 0.0 0.0 0.0 4.595824785099233E-5 0.0 7 0.0 0.0 0.0 4.595824785099233E-5 0.0 8 0.0 0.0 0.0 4.595824785099233E-5 0.0 9 0.0 0.0 0.0 4.595824785099233E-5 0.0 10 0.0 0.0 0.0 4.595824785099233E-5 0.0 11 0.0 0.0 0.0 4.595824785099233E-5 0.0 12 0.0 0.0 0.0 9.191649570198466E-5 0.0 13 0.0 0.0 0.0 9.191649570198466E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTACG 40 0.0052528316 14.261085 1 GGCGAGG 1145 0.0 12.681718 19 TAGGACC 875 0.0 12.286317 4 CTAGTCG 65 8.0772553E-4 11.683382 14 TTAGGAC 1550 0.0 11.100133 3 GTCCTAG 310 0.0 11.04084 1 CTAACAC 200 0.0 10.931484 3 GTCTAGG 185 5.456968E-12 10.792172 1 GTACTAA 185 5.456968E-12 10.792172 1 GTCCTAC 2395 0.0 10.678446 1 GACGTGA 1465 0.0 10.6399 7 TGTAGGA 2810 0.0 10.520476 2 CAGGACT 290 0.0 10.49792 4 CGTGCGC 100 2.394233E-5 10.452112 10 TGGCGAG 2630 0.0 10.428798 18 AGGACCT 1610 0.0 10.400114 5 GGACCTG 1580 0.0 10.35363 6 AGGACGT 2700 0.0 10.324171 5 GGACGTG 2630 0.0 10.270317 6 TCCAACG 130 3.8956932E-7 10.220611 18 >>END_MODULE