FastQCFastQC Report
Thu 26 May 2016
SRR1512986_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512986_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461428
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC48890.19862453827615514No Hit
CCCATGTACTCTGCGTTGATACCAC41410.16823567457589658No Hit
GTCCTACAGTGGACATTTCTAAATT33910.13776555722938066No Hit
CTGTAGGACGTGGAATATGGCAAGA32940.13382475538589794No Hit
GTATCAACGCAGAGTACTTTTTTTT31860.12943705848799963No Hit
GTCCTAAAGTGTGTATTTCTCATTT28630.11631459461743346No Hit
CTTTAGGACGTGAAATATGGCGAGG24820.10083577500540337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTG601.4765064E-615.8242087
CCGCTTA400.005272694414.25308114
TAGGACC4900.013.375824
TGGACCG802.8729492E-511.872985
TGTAGGA17800.010.5662622
GTCCTAA9500.010.5309641
GTAGGAC16500.010.4198633
GGCGAGG7550.010.18638619
AGGACGT17350.010.127965
GTAGAAC5600.010.0076323
TGCGTTT951.6411241E-410.00216212
GATATAC4100.09.9927961
GGACGTG16900.09.943976
TTAGGAC10250.09.8231183
TGGCGAG16100.09.79635118
TAAGGGT1951.6370905E-109.7423284
TATGGAC2151.0913936E-119.7196723
TAGGACG18900.09.6495454
CTGTAGG18150.09.6067581
GTCCTAC15500.09.5894861
Produced by FastQC (version 0.11.5)