##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512986_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2461428 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.73247521357521 31.0 30.0 33.0 22.0 34.0 2 28.877984243292918 31.0 30.0 34.0 22.0 34.0 3 29.030693564873722 31.0 30.0 34.0 23.0 34.0 4 32.5351913604623 35.0 33.0 37.0 25.0 37.0 5 32.6215745494079 35.0 33.0 37.0 25.0 37.0 6 32.764576497870344 36.0 33.0 37.0 25.0 37.0 7 32.671029174934226 36.0 33.0 37.0 25.0 37.0 8 32.679212229648805 37.0 35.0 37.0 25.0 37.0 9 34.020125309373256 39.0 35.0 39.0 25.0 39.0 10 33.882314656370205 38.0 34.0 39.0 23.0 39.0 11 33.95298013998378 38.0 34.0 39.0 25.0 39.0 12 33.83632631139322 38.0 34.0 39.0 23.0 39.0 13 33.847192767775454 38.0 34.0 39.0 23.0 39.0 14 34.84203153616519 39.0 34.0 41.0 22.0 41.0 15 34.766853225038474 39.0 34.0 41.0 22.0 41.0 16 34.66169881873449 39.0 34.0 41.0 19.0 41.0 17 34.59733455538817 39.0 34.0 41.0 19.0 41.0 18 34.297264433491456 39.0 34.0 41.0 17.0 41.0 19 34.521212483160184 39.0 34.0 41.0 18.0 41.0 20 34.60024627980181 39.0 34.0 41.0 18.0 41.0 21 34.54142676527609 39.0 34.0 41.0 18.0 41.0 22 34.451550482077884 39.0 34.0 41.0 18.0 41.0 23 34.19559012085667 39.0 34.0 41.0 11.0 41.0 24 34.190192034867565 39.0 34.0 41.0 10.0 41.0 25 34.10380681458081 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44185002312214294 1101 2 0.6523313638826878 1101 3 0.6451656985021827 1101 4 0.8040146371450234 1101 5 0.613723299681503 1101 6 0.7762978971713537 1101 7 0.7674363673985312 1101 8 0.674095764209909 1101 9 0.5295355717546073 1101 10 0.6080602557328731 1101 11 0.7452458332140921 1101 12 0.9705115502190012 1101 13 0.8237297105937174 1101 14 0.6598488405734315 1101 15 0.5851715081974476 1101 16 1.0762036066650964 1101 17 0.9941041809541602 1101 18 1.2024734096786673 1101 19 0.7822948928639732 1101 20 0.7999167254258452 1101 21 0.8368123665142946 1101 22 0.909335246945929 1101 23 1.1011180183761837 1101 24 0.7465122719310457 1101 25 0.9388502161394072 1102 1 0.50982293406868 1102 2 0.5215166882134277 1102 3 0.5555761915194211 1102 4 0.793169067996395 1102 5 0.6070952393093947 1102 6 0.5422146159186525 1102 7 0.7105832487680175 1102 8 0.7399907931382401 1102 9 0.76504076312834 1102 10 0.9256222277279349 1102 11 0.797728928282659 1102 12 0.8656677940964812 1102 13 0.7944904097499901 1102 14 0.8568287443756546 1102 15 0.9264061892680573 1102 16 1.0105262675256483 1102 17 0.8714032654828401 1102 18 1.1744858251210317 1102 19 1.1507427765432183 1102 20 1.069425985355764 1102 21 0.8520408886790065 1102 22 1.2027047590028417 1102 23 1.2846425163274588 1102 24 1.1458632661071206 1102 25 1.0293159575057302 1103 1 0.2218971025601455 1103 2 0.4149811890904189 1103 3 0.41018339701887285 1103 4 0.5525626904648249 1103 5 0.0665847317432906 1103 6 0.6192455884540848 1103 7 0.47995333951765673 1103 8 0.4895958742464117 1103 9 0.7343296912367592 1103 10 0.6515212810120303 1103 11 0.6971498954798747 1103 12 0.89132390417263 1103 13 0.7399507489989361 1103 14 0.9315310572385727 1103 15 0.9187256977165958 1103 16 1.123236119254031 1103 17 0.8832726208662649 1103 18 1.300584789071742 1103 19 1.2319762308034399 1103 20 1.0521367086292912 1103 21 0.8291764466313083 1103 22 0.9703726131497632 1103 23 1.3000007758518137 1103 24 1.0667650974413476 1103 25 1.0446360869301898 1104 1 0.4232491048190177 1104 2 0.355939753836914 1104 3 0.4561248349554248 1104 4 0.6885697481670334 1104 5 0.5099584258320178 1104 6 0.39460144408549525 1104 7 0.7008967897425862 1104 8 0.6976561432414599 1104 9 0.8135930275981025 1104 10 0.8873475521713985 1104 11 0.9006648841535636 1104 12 0.8443510885915089 1104 13 0.8332880302504151 1104 14 0.9310709778458062 1104 15 1.0056049386941979 1104 16 0.8397446136662623 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0.933949446290363 1107 17 1.031789243332831 1107 18 1.323308736241522 1107 19 1.0523272986697663 1107 20 0.9899558474741141 1107 21 0.9711266296883423 1107 22 0.9712543445826967 1107 23 1.1432425503949801 1107 24 1.1334323191320905 1107 25 1.1996419500027216 1108 1 0.36322313015656604 1108 2 0.43518176367511785 1108 3 0.2443727767430275 1108 4 0.5336683343889064 1108 5 0.48691448944543936 1108 6 0.6204454164118616 1108 7 0.7790050627416889 1108 8 0.7589222289653819 1108 9 0.8211496814533703 1108 10 0.7140872545120729 1108 11 0.6328578204779447 1108 12 0.7715766063411778 1108 13 0.7116444259926809 1108 14 0.9758993245340406 1108 15 0.9426775804128695 1108 16 0.839539467868228 1108 17 1.0490131975409156 1108 18 1.1314968854880831 1108 19 1.074501556297946 1108 20 0.9483623807828323 1108 21 0.8794151249350577 1108 22 0.7539378854565655 1108 23 1.0357429275609533 1108 24 1.0565675063354476 1108 25 1.1591369297250864 1109 1 -0.15244789459182329 1109 2 -0.03173285396184511 1109 3 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-1.1474481815666309 2215 6 -1.6932956023663834 2215 7 -2.1712172175297866 2215 8 -2.1919073570812344 2215 9 -1.8275327732284765 2215 10 -3.425211192601253 2215 11 -2.4809257677538135 2215 12 -2.6446180991521473 2215 13 -1.9837864756988353 2215 14 -3.197575483125977 2215 15 -3.113081976809525 2215 16 -2.802144803617079 2215 17 -3.021568960467775 2215 18 -1.889641059582118 2215 19 -2.4408668456959077 2215 20 -2.6129551161609434 2215 21 -2.278307741011936 2215 22 -1.9590782086993244 2215 23 -2.5083431318414036 2215 24 -2.4864057350312834 2215 25 -2.387642269568371 2216 1 -0.8285665186444255 2216 2 -0.7648900017381521 2216 3 -1.2290570090513633 2216 4 -2.100260966663029 2216 5 -1.0856738254155367 2216 6 -0.9775631808111989 2216 7 -1.0076624687983653 2216 8 -1.5401649798433539 2216 9 -1.5400088864053245 2216 10 -1.2793629194006115 2216 11 -1.7547188138227412 2216 12 -2.142644487730017 2216 13 -2.4457545817558852 2216 14 -1.9142981623092368 2216 15 -1.7567992665767136 2216 16 -1.0124733818609712 2216 17 -1.0886970628781896 2216 18 -1.0928849764634947 2216 19 -1.4976745128461388 2216 20 -1.2549941546869903 2216 21 -1.7443555704199696 2216 22 -2.1030742186373033 2216 23 -2.166916144754225 2216 24 -2.04624002531272 2216 25 -2.103144828855047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 121623.0 3 4.0 4 966.0 5 5694.0 6 6902.0 7 6390.0 8 5740.0 9 5198.0 10 5071.0 11 4710.0 12 4880.0 13 4539.0 14 4522.0 15 4599.0 16 4770.0 17 5100.0 18 5648.0 19 6826.0 20 8970.0 21 11263.0 22 14293.0 23 17242.0 24 19683.0 25 22708.0 26 25505.0 27 28359.0 28 32421.0 29 38088.0 30 46874.0 31 58500.0 32 74057.0 33 94744.0 34 122589.0 35 162723.0 36 225486.0 37 362896.0 38 891845.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.62423878688642 18.409615014531138 12.60592563840787 25.360220560174568 2 15.02426165439325 19.699227829513667 40.8750046231061 24.401505892986982 3 19.2173358205619 24.04325014122744 29.1530417748733 27.586372263337356 4 12.472674025663148 16.469876301069956 37.75046523754039 33.306984435726505 5 13.548685234092467 37.69629635048719 35.43080540944336 13.324213005976985 6 32.777179785055814 36.40495714972424 17.82503207015328 12.992830995066662 7 28.597921673063748 31.28949077170504 21.706058224685883 18.406529330545325 8 25.663169127378783 36.363680693802564 20.227699682185957 17.7454504966327 9 26.5570746197091 15.266655695271533 19.717344353490223 38.458925331529144 10 15.079239627900249 28.753454454432898 34.236411666537315 21.930894251129544 11 34.65457655838259 22.394507845499632 24.018504936071825 18.932410660045957 12 23.421569973969486 24.419880388044003 31.27440614589928 20.88414349208723 13 28.96909503755439 21.611165380720728 25.46672767257059 23.9530119091543 14 21.27119959622526 21.758728337135654 27.22419494972728 29.745877116911807 15 23.642279766299936 30.0172588886121 23.016172639277034 23.324288705810925 16 22.685257989551115 27.935461875638225 27.034990387791947 22.34428974701871 17 22.315702214031887 28.240294689533684 27.28617027544857 22.157832820985863 18 22.303658449306763 27.385506737194127 28.733311537485605 21.57752327601351 19 23.71315753221754 27.036604033811095 27.15607499688116 22.09416343709021 20 23.81803213138629 27.100657646645775 27.431512749827704 21.64979747214023 21 23.327403046309065 26.750660553784233 26.894027748541742 23.027908651364957 22 23.37301008423098 27.57001199932872 26.60559592128473 22.451381995155568 23 22.786181450244726 27.41648871650355 26.95694046271783 22.84038937053389 24 23.273444350625233 26.90814864538695 27.483524976452646 22.334882027535173 25 23.075900933482657 26.900692919477976 27.11760613631155 22.905800010727816 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 188.0 1 188.0 2 373.0 3 558.0 4 558.0 5 558.0 6 1044.0 7 1530.0 8 1530.0 9 1530.0 10 2491.0 11 3452.0 12 3452.0 13 3452.0 14 6216.0 15 8980.0 16 8980.0 17 8980.0 18 16737.5 19 24495.0 20 24495.0 21 24495.0 22 39273.0 23 54051.0 24 54051.0 25 54051.0 26 77531.5 27 101012.0 28 101012.0 29 101012.0 30 133385.5 31 165759.0 32 165759.0 33 165759.0 34 191715.5 35 217672.0 36 217672.0 37 217672.0 38 240081.0 39 262490.0 40 262490.0 41 262490.0 42 281663.5 43 300837.0 44 300837.0 45 300837.0 46 318764.5 47 336692.0 48 336692.0 49 336692.0 50 326374.5 51 316057.0 52 316057.0 53 316057.0 54 283768.5 55 251480.0 56 251480.0 57 251480.0 58 217557.5 59 183635.0 60 183635.0 61 183635.0 62 150672.5 63 117710.0 64 117710.0 65 117710.0 66 91778.0 67 65846.0 68 65846.0 69 65846.0 70 48225.5 71 30605.0 72 30605.0 73 30605.0 74 21280.0 75 11955.0 76 11955.0 77 11955.0 78 8211.0 79 4467.0 80 4467.0 81 4467.0 82 2928.5 83 1390.0 84 1390.0 85 1390.0 86 896.5 87 403.0 88 403.0 89 403.0 90 258.5 91 114.0 92 114.0 93 114.0 94 72.5 95 31.0 96 31.0 97 31.0 98 25.0 99 19.0 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3560534778998208 2 0.03904237702666907 3 0.03485781424441422 4 0.034817187421285534 5 0.04022055489740102 6 0.016941385244662856 7 0.01665699748276204 8 0.022547886836421782 9 0.019622755571156256 10 0.019297740986126754 11 0.018810219108582497 12 0.019094606870483314 13 0.018850845931711185 14 0.026407435033647137 15 0.02709809102683483 16 0.02011027744870051 17 0.021654096727590652 18 0.09116659110077566 19 0.023522930591510297 20 0.021613469904461963 21 0.024944869401014372 22 0.01856645816981037 23 0.021654096727590652 24 0.017469533945335798 25 0.021775977196976715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2461428.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.6890772439255 #Duplication Level Percentage of deduplicated Percentage of total 1 80.47372930724117 55.27666208491839 2 12.651006298390936 17.37971897687126 3 3.591195630154756 7.400277421132433 4 1.3535862742012192 3.7190636857969945 5 0.6552906259396027 2.2505654211192834 6 0.3717801096359356 1.532233960112272 7 0.2279839724443089 1.096200608552288 8 0.1368353495267659 0.7519275114674853 9 0.09552375302934105 0.5905294605415863 >10 0.40959969220885534 4.635747115057435 >50 0.01779496162664353 0.8501021127319073 >100 0.012117798252160314 1.6658058052539806 >500 0.002430088687998328 1.1591207238118195 >1k 0.0011261386602739835 1.6920451126328482 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 4889 0.19862453827615514 No Hit CCCATGTACTCTGCGTTGATACCAC 4141 0.16823567457589658 No Hit GTCCTACAGTGGACATTTCTAAATT 3391 0.13776555722938066 No Hit CTGTAGGACGTGGAATATGGCAAGA 3294 0.13382475538589794 No Hit GTATCAACGCAGAGTACTTTTTTTT 3186 0.12943705848799963 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2863 0.11631459461743346 No Hit CTTTAGGACGTGAAATATGGCGAGG 2482 0.10083577500540337 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.06268231286879E-5 0.0 6 0.0 0.0 0.0 4.06268231286879E-5 0.0 7 0.0 0.0 0.0 4.06268231286879E-5 0.0 8 0.0 0.0 0.0 8.12536462573758E-5 0.0 9 0.0 0.0 0.0 8.12536462573758E-5 0.0 10 0.0 0.0 0.0 8.12536462573758E-5 0.0 11 0.0 0.0 0.0 1.218804693860637E-4 0.0 12 0.0 0.0 0.0 1.218804693860637E-4 0.0 13 0.0 0.0 0.0 1.218804693860637E-4 4.06268231286879E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCGTG 60 1.4765064E-6 15.824208 7 CCGCTTA 40 0.0052726944 14.253081 14 TAGGACC 490 0.0 13.37582 4 TGGACCG 80 2.8729492E-5 11.87298 5 TGTAGGA 1780 0.0 10.566262 2 GTCCTAA 950 0.0 10.530964 1 GTAGGAC 1650 0.0 10.419863 3 GGCGAGG 755 0.0 10.186386 19 AGGACGT 1735 0.0 10.12796 5 GTAGAAC 560 0.0 10.007632 3 TGCGTTT 95 1.6411241E-4 10.002162 12 GATATAC 410 0.0 9.992796 1 GGACGTG 1690 0.0 9.94397 6 TTAGGAC 1025 0.0 9.823118 3 TGGCGAG 1610 0.0 9.796351 18 TAAGGGT 195 1.6370905E-10 9.742328 4 TATGGAC 215 1.0913936E-11 9.719672 3 TAGGACG 1890 0.0 9.649545 4 CTGTAGG 1815 0.0 9.606758 1 GTCCTAC 1550 0.0 9.589486 1 >>END_MODULE