FastQCFastQC Report
Thu 26 May 2016
SRR1512986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512986_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461428
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC48800.19825889686799694No Hit
CCCATGTACTCTGCGTTGATACCAC47640.19354618538506915No Hit
GTATCAACGCAGAGTACTTTTTTTT40900.1661637065963335No Hit
GTCCTACAGTGGACATTTCTAAATT31770.12907141707984146No Hit
CTGTAGGACGTGGAATATGGCAAGA31300.12716195639279312No Hit
TATCAACGCAGAGTACTTTTTTTTT27230.11062683937941714No Hit
GTCCTAAAGTGTGTATTTCTCATTT26270.10672666435906311No Hit
CTTTAGGACGTGAAATATGGCGAGG26050.10583287425023197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT1207.2577677E-1012.6830246
GGCGAGG8000.011.62530619
GCCGGTT907.535964E-611.60130111
CCGGTTT907.535964E-611.60130112
TAGGACC5450.011.5231994
AATCCCG2500.010.62885119
TCCAACG1701.4188117E-1010.60630518
TGGCGAG16250.010.45343718
GTATTAG1553.614332E-910.4281391
AATGCCG2100.010.39364818
AGGACGT17250.010.2048885
GTCCTAC15050.010.1713581
GATATAC5150.010.1541841
GGACGTG16800.010.1350966
TTAGACT1606.015398E-910.1100794
AGTCGTC1606.1354513E-910.0979438
GTAGGAC16200.010.0945123
GACGTGA9550.09.9521827
CCAACGA1054.15443E-59.94195219
TATACTG3450.09.9290815
Produced by FastQC (version 0.11.5)