##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512986_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2461428
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.404030505868953	31.0	31.0	34.0	27.0	34.0
2	30.571355327070304	31.0	31.0	34.0	27.0	34.0
3	30.66140102412096	31.0	31.0	34.0	27.0	34.0
4	34.127623070835305	37.0	35.0	37.0	30.0	37.0
5	33.78782722874689	37.0	35.0	37.0	28.0	37.0
6	33.653478793610866	37.0	35.0	37.0	28.0	37.0
7	33.5270859029799	37.0	35.0	37.0	28.0	37.0
8	33.407586977965636	37.0	35.0	37.0	27.0	37.0
9	34.86124274201805	39.0	35.0	39.0	27.0	39.0
10	34.74579106112387	39.0	35.0	39.0	27.0	39.0
11	34.77798091189342	39.0	35.0	39.0	27.0	39.0
12	34.624973795699084	39.0	35.0	39.0	27.0	39.0
13	34.609683484546366	39.0	35.0	39.0	27.0	39.0
14	35.66462963775499	40.0	36.0	41.0	27.0	41.0
15	35.663862197066095	40.0	36.0	41.0	27.0	41.0
16	35.62996236331105	40.0	36.0	41.0	27.0	41.0
17	35.6053091945001	40.0	36.0	41.0	27.0	41.0
18	35.52824092356145	40.0	36.0	41.0	27.0	41.0
19	35.50567841106869	40.0	36.0	41.0	26.0	41.0
20	35.48391096550458	40.0	36.0	41.0	26.0	41.0
21	35.425463592678724	40.0	36.0	41.0	26.0	41.0
22	35.390184478278464	40.0	36.0	41.0	26.0	41.0
23	35.277136686508804	40.0	36.0	41.0	25.0	41.0
24	35.25163401082624	40.0	36.0	41.0	25.0	41.0
25	35.152134858301764	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.693359281669391
1101	2	0.7290926409692204
1101	3	0.7222430536881888
1101	4	0.5858007179170457
1101	5	0.8046539066548206
1101	6	0.8792331098485846
1101	7	0.8752405286985194
1101	8	0.8953889140959319
1101	9	0.7561361035761678
1101	10	1.0642362309631181
1101	11	1.0276164519637305
1101	12	1.101550740563674
1101	13	1.2093938815105147
1101	14	1.2781327663955295
1101	15	1.2683963941537684
1101	16	1.2803108081639536
1101	17	1.2113038424692562
1101	18	0.7112563440105646
1101	19	1.0517330945056216
1101	20	1.023889255831321
1101	21	1.2328304225132456
1101	22	1.0777404632450143
1101	23	1.0101625784242003
1101	24	1.0641920928591801
1101	25	1.0635982904001082
1102	1	0.7988021464775699
1102	2	0.709614682192278
1102	3	0.6760956550086021
1102	4	0.6544062485834061
1102	5	0.7370591822415165
1102	6	0.7172767884471583
1102	7	0.7499857162039731
1102	8	0.7462194929937738
1102	9	0.8776220461591038
1102	10	1.0623308516109589
1102	11	0.9842957881761052
1102	12	1.024724122036794
1102	13	1.1255698556030538
1102	14	1.157572203315162
1102	15	1.0943119149475606
1102	16	1.056978433985023
1102	17	1.001798859813853
1102	18	1.1171065657836863
1102	19	1.2078117916071704
1102	20	1.1479852989829809
1102	21	1.1617145327814384
1102	22	1.1517162690699436
1102	23	1.1567411612056233
1102	24	1.1226635041869386
1102	25	1.2277208231007393
1103	1	0.6827068865937029
1103	2	0.595025561254495
1103	3	0.6692758142834769
1103	4	0.6828240673238071
1103	5	0.6696724014024369
1103	6	0.7548431075851951
1103	7	0.8144255850364246
1103	8	0.7942852074973743
1103	9	1.0045871080932827
1103	10	1.0086572888433665
1103	11	1.0519821626572678
1103	12	1.0597302773243484
1103	13	1.0659398265152333
1103	14	1.1436247574871388
1103	15	0.9882012940805964
1103	16	1.0555131487174023
1103	17	1.2429363351654672
1103	18	1.204658722313198
1103	19	1.3239124986481627
1103	20	1.3380856356172899
1103	21	0.9932339627909101
1103	22	1.2542208719177168
1103	23	1.2373754281816858
1103	24	1.2475668171479057
1103	25	1.3007127286252498
1104	1	0.6886099824514531
1104	2	0.7771552267291213
1104	3	0.6612781498156366
1104	4	0.7394324862273081
1104	5	0.7020652614199889
1104	6	0.80231490680098
1104	7	0.8566518275765844
1104	8	0.8096377540323374
1104	9	1.0337454221661417
1104	10	1.024396003099696
1104	11	0.9382879972714377
1104	12	0.994855952718595
1104	13	1.038427947625614
1104	14	1.2059370237582527
1104	15	1.2687816252713162
1104	16	1.2446664672045173
1104	17	1.0510070169543226
1104	18	1.212997389807775
1104	19	1.1566112264986188
1104	20	1.2376235119950678
1104	21	1.0622638864772753
1104	22	1.2203861913094016
1104	23	1.0598475148672364
1104	24	1.1366128135883926
1104	25	1.4164684994211285
1105	1	0.5200555400113629
1105	2	0.5944865673797821
1105	3	0.5863531126901087
1105	4	0.47232541728676836
1105	5	0.4196300800253354
1105	6	0.5757394209738536
1105	7	0.635242974775899
1105	8	0.7210903222582061
1105	9	0.7712894365569838
1105	10	0.7921694649450259
1105	11	0.7954327706870927
1105	12	0.7491784269015653
1105	13	0.7971134224495344
1105	14	0.9791312236343614
1105	15	0.7999842771759376
1105	16	0.9041411481338031
1105	17	0.6370511727606782
1105	18	0.8741399511938113
1105	19	0.9868314993451364
1105	20	0.9631317061854574
1105	21	0.9227776392146296
1105	22	0.9919371239873982
1105	23	0.994921977883763
1105	24	1.072831128763383
1105	25	1.0875980328638448
1106	1	0.4270024792988707
1106	2	0.4718563616597642
1106	3	0.40692348398965805
1106	4	0.4132288292285651
1106	5	0.4054041643203661
1106	6	0.42717745408975816
1106	7	0.441428624550106
1106	8	0.4664085610941271
1106	9	0.510599772733606
1106	10	0.7057897609811548
1106	11	0.36669594178594167
1106	12	0.569409861924143
1106	13	0.5627280737290192
1106	14	0.8110003907191583
1106	15	0.7194373629762367
1106	16	0.8338606664478974
1106	17	0.6249953523893765
1106	18	0.8257949938430826
1106	19	0.7754687296512017
1106	20	0.8931550378462703
1106	21	0.9056838108152263
1106	22	0.7949703653321052
1106	23	0.9363503521938483
1106	24	0.8072653987081893
1106	25	0.8476027995472322
1107	1	0.5155313637610206
1107	2	0.5349101465203212
1107	3	0.5221535636709334
1107	4	0.6237626937704022
1107	5	0.588933048782522
1107	6	0.6208547449264117
1107	7	0.56584695124333
1107	8	0.7558119475483096
1107	9	0.6822392149646817
1107	10	0.7416294024154197
1107	11	0.7327118780409165
1107	12	0.6852514953902826
1107	13	0.7269611414580979
1107	14	0.8451368449024272
1107	15	0.7954732195499403
1107	16	0.7701475190375433
1107	17	0.6062334001981426
1107	18	0.8999404121697765
1107	19	1.0247595663045885
1107	20	0.9938801374478601
1107	21	0.982231619580169
1107	22	1.0608398872444553
1107	23	1.0425067266958408
1107	24	1.121886912652414
1107	25	1.1306147517384701
1108	1	0.6445138146252276
1108	2	0.5531209356507958
1108	3	0.5770264984390856
1108	4	0.6517542402898542
1108	5	0.6538584493228612
1108	6	0.5733737669945924
1108	7	0.6097036619193119
1108	8	0.7389144156033467
1108	9	0.6408665888771026
1108	10	0.8975412557494664
1108	11	0.8094101919105015
1108	12	0.9202923980477138
1108	13	1.014830714486365
1108	14	1.0678091849393851
1108	15	1.0278429434445968
1108	16	0.9549025199606049
1108	17	1.0646927863704434
1108	18	0.9795809928465715
1108	19	1.0399201496811017
1108	20	0.9945683412842357
1108	21	1.1348477370733292
1108	22	1.1444697425119728
1108	23	1.1175993556816408
1108	24	1.1205327131077922
1108	25	1.1416683202248592
1109	1	0.12500807115612034
1109	2	0.17623082760598052
1109	3	0.15392266720152037
1109	4	0.28174818082444375
1109	5	0.24746416683355932
1109	6	-0.1470241040906899
1109	7	0.030223799217104386
1109	8	0.4276405917551003
1109	9	0.3575873324219714
1109	10	0.20490115831717048
1109	11	0.21977900285656204
1109	12	0.30485501145920324
1109	13	0.2691593378657302
1109	14	0.36962171517253495
1109	15	0.3563034097216544
1109	16	0.22936380794073585
1109	17	0.30659418375404357
1109	18	0.431865488942357
1109	19	0.47809200183014156
1109	20	0.6312526754436547
1109	21	0.508273406190952
1109	22	0.5840289855884464
1109	23	0.44683030946954005
1109	24	0.11923260152296677
1109	25	0.27658784604133047
1110	1	0.289841956929461
1110	2	0.30690110047505925
1110	3	0.27720222680395423
1110	4	0.42585854679917645
1110	5	0.3061485407262694
1110	6	0.3032054470915071
1110	7	0.3044888594913715
1110	8	0.4927026750656367
1110	9	0.4692867259952891
1110	10	0.5829756520084004
1110	11	0.4196658243153095
1110	12	0.512896177845164
1110	13	0.20529493201444637
1110	14	0.5342272714989633
1110	15	0.6443836264103808
1110	16	0.6740588668977878
1110	17	0.4972406035765857
1110	18	0.48331448896930596
1110	19	0.5844719788389057
1110	20	0.6289009763616349
1110	21	0.5499667400829154
1110	22	0.5979997954745713
1110	23	0.6408980277749521
1110	24	0.5643342564937441
1110	25	0.5849430894747485
1111	1	-0.22815613960040437
1111	2	-0.08787157188125505
1111	3	-0.030723712272024528
1111	4	-0.06228051656582778
1111	5	0.249905462347364
1111	6	0.07042148316556052
1111	7	0.22455635040680022
1111	8	0.24418452163411075
1111	9	-0.1580300299662909
1111	10	-0.060531890109189135
1111	11	0.21482418685343418
1111	12	0.10198125340908604
1111	13	0.13238539460129317
1111	14	0.4017711591511386
1111	15	0.5709223058630499
1111	16	0.16519919188922927
1111	17	0.49460049257629635
1111	18	0.36970620879635874
1111	19	0.49414578053386293
1111	20	0.32553146567596514
1111	21	0.18505868101252787
1111	22	0.05762067685858341
1111	23	0.2840925640318517
1111	24	0.41531057004775107
1111	25	0.34901038002490736
1112	1	-0.0025262840057429514
1112	2	-0.15276502708833561
1112	3	-0.0627175449003552
1112	4	-0.11507857067006455
1112	5	0.0662187867227999
1112	6	0.05709331825600117
1112	7	-0.05847406171446323
1112	8	0.06069429348135458
1112	9	-0.2137162434853508
1112	10	0.06728988769175004
1112	11	-0.055340297443663644
1112	12	0.08334992781365003
1112	13	0.10754814468694462
1112	14	0.0775841464211311
1112	15	-0.02266603286765445
1112	16	-0.32127922204677617
1112	17	-0.1943344752842151
1112	18	-0.08771666353147367
1112	19	-0.04900871586323774
1112	20	0.21022219446056312
1112	21	0.2972438716363115
1112	22	0.13158453157640082
1112	23	0.21553344900073768
1112	24	0.10230974741573817
1112	25	0.1582163322385668
1113	1	-0.5089054841476042
1113	2	-0.8332036905595572
1113	3	-0.38171685543592915
1113	4	-0.4646706485782204
1113	5	-0.27483147536631947
1113	6	-0.40388259812956306
1113	7	-0.21880114046295063
1113	8	-0.22170305770482912
1113	9	-0.19804383039955553
1113	10	-0.4497004478961273
1113	11	-0.3169920477701993
1113	12	-0.3084197725406099
1113	13	-0.20690639102294028
1113	14	-0.22352376071687274
1113	15	-0.139492140940213
1113	16	-0.5521993857714236
1113	17	-0.3032167886027892
1113	18	-0.1629869904911203
1113	19	-0.20514223640623896
1113	20	-0.04243634596574708
1113	21	-0.29533968787928444
1113	22	-0.4419819845373496
1113	23	-0.2062586556390542
1113	24	-0.38686645507771544
1113	25	-0.5583358427504166
1114	1	-0.49510466355827276
1114	2	-0.3545578709477759
1114	3	-0.4269980507952589
1114	4	-0.17900988558821496
1114	5	-0.36796559138861085
1114	6	-0.7332558969458205
1114	7	-0.47767280933199885
1114	8	-0.9652015026339669
1114	9	-0.646946961201607
1114	10	-0.8133965109253793
1114	11	-0.6715953487079531
1114	12	-0.6719516622394224
1114	13	-0.5750679963718852
1114	14	-1.6302092481549764
1114	15	-1.020376763417815
1114	16	-0.9545413516083912
1114	17	-1.0472458849977713
1114	18	-0.9952636891096489
1114	19	-1.0055583547885192
1114	20	-0.33503481876552144
1114	21	-0.395055435068997
1114	22	-0.521666782835716
1114	23	-0.44800665789643546
1114	24	-1.1642176071335513
1114	25	-0.9930899779355045
1115	1	-1.7648838080928009
1115	2	-1.602765388410127
1115	3	-1.3114888028428808
1115	4	-2.4863586742659827
1115	5	-1.678325147952485
1115	6	-1.4788678352588605
1115	7	-1.1685775258552624
1115	8	-1.2343919001862744
1115	9	-1.6000654495196827
1115	10	-1.5258505101958093
1115	11	-1.289286026343781
1115	12	-1.8954884467143813
1115	13	-1.4179624490554374
1115	14	-2.3552862321491688
1115	15	-2.2306129585222223
1115	16	-1.632664074955514
1115	17	-1.7143741611694665
1115	18	-1.5499714305348888
1115	19	-1.406165600793301
1115	20	-1.5302021050108792
1115	21	-1.6099013163863347
1115	22	-1.837256989993591
1115	23	-2.262612675482977
1115	24	-1.5102485794858325
1115	25	-1.3147303008162012
1116	1	-1.451124626128454
1116	2	-1.5113364019085473
1116	3	-1.4568595790172019
1116	4	-1.6213971844956987
1116	5	-1.4562085930096416
1116	6	-2.0707863637517896
1116	7	-1.4735982001535746
1116	8	-1.774384795112983
1116	9	-1.8658696595808095
1116	10	-1.87838849853226
1116	11	-1.807790957777378
1116	12	-2.4067808741956327
1116	13	-2.12240604978939
1116	14	-2.7862595905659333
1116	15	-2.598635001340668
1116	16	-2.7963957278128575
1116	17	-2.624374734946862
1116	18	-3.376010059182491
1116	19	-2.6748332042227787
1116	20	-2.4344441511743184
1116	21	-2.420312446202864
1116	22	-3.8990644132081336
1116	23	-3.812192536140529
1116	24	-2.9169407772119627
1116	25	-3.4590749920934414
1201	1	0.7041853035287424
1201	2	0.7791517279553872
1201	3	0.6839156532881319
1201	4	0.7192563984752667
1201	5	0.7524607912942187
1201	6	0.8213384688451271
1201	7	0.7354363905350922
1201	8	0.8247371269755703
1201	9	1.0449537722158695
1201	10	0.8970807912685004
1201	11	0.9155562834892805
1201	12	0.8775230511553147
1201	13	0.9260027307313479
1201	14	0.8556773904602935
1201	15	1.0310423332066279
1201	16	1.05086273737723
1201	17	0.959477555340456
1201	18	1.0285484238197853
1201	19	0.9119911215745802
1201	20	1.0990645641211856
1201	21	0.9848160914261541
1201	22	1.1255055064141644
1201	23	1.153888729102981
1201	24	1.1791389425860004
1201	25	1.0382591547116107
1202	1	0.8500054234872962
1202	2	0.9244124292480898
1202	3	0.9280157778964693
1202	4	0.9293678461256505
1202	5	0.989491337899473
1202	6	1.107189742876237
1202	7	0.9348295462090945
1202	8	0.8655231284264389
1202	9	1.2642067875825518
1202	10	1.215940682640145
1202	11	1.2118943544843503
1202	12	1.1496601179405275
1202	13	1.1816374439287074
1202	14	1.1872701039808078
1202	15	1.3690174961047603
1202	16	1.215964609100233
1202	17	1.4393308618619827
1202	18	1.4094772775092395
1202	19	1.585691975938964
1202	20	1.6515695283736633
1202	21	1.5037524232064214
1202	22	1.438710636709473
1202	23	1.4685691181948712
1202	24	1.300338875410695
1202	25	1.318094607022914
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2211	10	-0.4738158397300438
2211	11	-0.2936412438884304
2211	12	-0.20198745837049614
2211	13	-0.21891530955775096
2211	14	-1.1173047273910797
2211	15	-0.7079769118523132
2211	16	-0.46608319943982934
2211	17	-0.19917400244933248
2211	18	-0.4694390676250393
2211	19	-0.41573929676484767
2211	20	-0.546603280670368
2211	21	-0.478590509733408
2211	22	-0.30358367327597335
2211	23	-0.34010399121483914
2211	24	-0.39670907424076063
2211	25	-0.5255761438106532
2212	1	-0.2962783294302689
2212	2	-0.26253901703086413
2212	3	-0.26456592550970015
2212	4	-0.08556642109235923
2212	5	-0.23572966109683335
2212	6	-0.31267877184092185
2212	7	-0.3598345333446318
2212	8	-0.5916886789572189
2212	9	-0.5952237189223624
2212	10	-0.6200584418429784
2212	11	-0.5411121455054584
2212	12	-0.42913153730563636
2212	13	-0.511867561701024
2212	14	-0.6026655271242305
2212	15	-0.4224239977426336
2212	16	-0.45406359542629815
2212	17	-0.6324093331048459
2212	18	-0.45436611013637673
2212	19	-0.3545210007724151
2212	20	-0.6815683254399971
2212	21	-0.7034253034338107
2212	22	-0.7251012007782975
2212	23	-1.0202610085250257
2212	24	-0.6847532148990041
2212	25	-0.7761051593744597
2213	1	-0.3626614754004258
2213	2	-0.3469996654165186
2213	3	-0.2657281041384323
2213	4	-0.2958335243251966
2213	5	-0.25370589816336064
2213	6	-0.02559317855799037
2213	7	-0.6365552338201113
2213	8	-0.32693620589363803
2213	9	-0.5990939268238051
2213	10	-0.37379869254657194
2213	11	-0.42327216547779756
2213	12	-0.6358260792370132
2213	13	-0.4124580757112355
2213	14	-0.3510119107648322
2213	15	-0.5190135612622413
2213	16	-0.6640948138957583
2213	17	-0.6068887272773722
2213	18	-0.8003295830900043
2213	19	-1.145566938143638
2213	20	-0.5565717260173102
2213	21	-1.2912399069665952
2213	22	-0.6711580517467794
2213	23	-0.7934972786314631
2213	24	-0.9811145804639025
2213	25	-0.9226813712416231
2214	1	-1.103269040722921
2214	2	-1.1205179232762212
2214	3	-0.958412187489504
2214	4	-1.7055027153097058
2214	5	-1.4624640595968188
2214	6	-2.7864024968772796
2214	7	-1.4191586813610897
2214	8	-2.0437262114474137
2214	9	-1.5415490967842373
2214	10	-1.7592679208494104
2214	11	-1.5757700075466659
2214	12	-1.6382716281330616
2214	13	-1.836179580861014
2214	14	-1.9671300233369564
2214	15	-2.313684402210363
2214	16	-1.6766969472813287
2214	17	-1.7620161567362018
2214	18	-1.8192552649419866
2214	19	-3.474173874543837
2214	20	-2.1176636764005323
2214	21	-2.6081554700346743
2214	22	-2.2142714625077247
2214	23	-2.29997142661302
2214	24	-1.6072517628281773
2214	25	-1.381600823443776
2215	1	-1.7644527911588277
2215	2	-1.6545042486037609
2215	3	-1.5997821332086772
2215	4	-1.5470880917145706
2215	5	-2.2486620631414915
2215	6	-2.116306595840893
2215	7	-2.4143494614415744
2215	8	-3.227651425036246
2215	9	-2.5976020303177165
2215	10	-2.120616965787221
2215	11	-2.5493477475344477
2215	12	-3.3554323710855627
2215	13	-2.752237252207184
2215	14	-2.829539484454216
2215	15	-3.9275667979114175
2215	16	-3.4169118716935714
2215	17	-3.0942927890808036
2215	18	-3.739398487432922
2215	19	-4.517926554676119
2215	20	-3.922712507230017
2215	21	-3.0786490601972503
2215	22	-4.160839967538909
2215	23	-3.9670126852541436
2215	24	-2.9358240603764187
2215	25	-3.249181527775928
2216	1	-1.4699884268913017
2216	2	-1.3188660378297072
2216	3	-1.728439151619785
2216	4	-1.1956668312692003
2216	5	-1.331215012450535
2216	6	-1.7903107399115186
2216	7	-3.0975196843203463
2216	8	-2.23990334062891
2216	9	-2.325976168555485
2216	10	-2.310984170097335
2216	11	-2.822054855916676
2216	12	-2.2345650902136995
2216	13	-2.8648596735587937
2216	14	-2.8859458441244925
2216	15	-2.906554979078642
2216	16	-2.739265689646551
2216	17	-2.830944181752294
2216	18	-2.7150291925002463
2216	19	-2.929653159308593
2216	20	-3.4530756577504143
2216	21	-3.182387663339206
2216	22	-3.428504267724694
2216	23	-2.5784187926689484
2216	24	-2.8622129037806445
2216	25	-2.8423464665663616
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	64330.0
3	0.0
4	1039.0
5	9236.0
6	10672.0
7	8369.0
8	6744.0
9	5977.0
10	5142.0
11	4832.0
12	4383.0
13	4224.0
14	3969.0
15	4065.0
16	4195.0
17	4305.0
18	4709.0
19	5724.0
20	7150.0
21	9314.0
22	11826.0
23	14408.0
24	17026.0
25	19612.0
26	22414.0
27	24734.0
28	28005.0
29	33915.0
30	42514.0
31	54330.0
32	68649.0
33	89079.0
34	116285.0
35	152119.0
36	208540.0
37	326349.0
38	1063244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.8094611577479	19.023431512499638	13.753648531784869	27.4134587979676
2	13.769202267951774	20.223057509705747	39.55866269498844	26.449077527354042
3	17.381333112323418	24.131886043386196	29.78811486665464	28.698665977635745
4	11.729112975519682	17.007689263070976	36.87518134096002	34.38801642044933
5	13.042311695069596	37.85510439703395	35.71313581371146	13.389448094184988
6	32.76413640558991	36.86228109765271	17.40948105861927	12.964101438138107
7	28.266564364072604	31.363073694443067	21.74363609320048	18.626725848283847
8	25.647003347567782	36.756416171577584	19.874430639086665	17.72214984176797
9	26.127317990143776	15.0707920540568	19.506904435299496	39.29498552049993
10	14.650981387354983	29.130908313019088	34.293938750627326	21.924171548998604
11	34.627688662419146	22.31852874474781	23.931299601763907	19.12248299106914
12	23.09695118879096	24.585113662035237	31.357337771356224	20.960597377817578
13	28.77868686614245	21.51111721443552	25.615422306260307	24.09477361316173
14	21.114912220114007	21.618839054634396	27.209530039369916	30.056718685881677
15	23.487183737613694	30.076703773269838	23.017348240981242	23.418764248135222
16	22.637067400445012	27.89620048662117	26.957861922462428	22.50887019047139
17	22.158226896459237	28.25855321708246	27.293448798546798	22.289771087911507
18	22.18970694403648	27.4702233316148	28.6390263780025	21.701043346346218
19	23.593644494389693	26.956254301480236	27.294766068542536	22.15533513558753
20	23.69488729624754	27.0125786163522	27.446167097329887	21.84636699007037
21	23.061022350458636	26.682865948438018	27.05387039870384	23.202241302399504
22	23.225689550873255	27.380078418117016	26.801794573134785	22.592437457874944
23	22.509697622680818	27.218833945173287	27.170122384241992	23.1013460479039
24	23.087907949331832	27.01128247963478	27.535287457235174	22.365522113798214
25	23.017768668071042	26.776430055802596	27.218503629098606	22.987297647027756
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	101.0
1	101.0
2	255.5
3	410.0
4	410.0
5	410.0
6	1020.0
7	1630.0
8	1630.0
9	1630.0
10	2480.0
11	3330.0
12	3330.0
13	3330.0
14	6125.5
15	8921.0
16	8921.0
17	8921.0
18	16550.5
19	24180.0
20	24180.0
21	24180.0
22	39386.5
23	54593.0
24	54593.0
25	54593.0
26	78813.0
27	103033.0
28	103033.0
29	103033.0
30	134769.0
31	166505.0
32	166505.0
33	166505.0
34	192026.5
35	217548.0
36	217548.0
37	217548.0
38	240481.5
39	263415.0
40	263415.0
41	263415.0
42	282351.5
43	301288.0
44	301288.0
45	301288.0
46	319605.5
47	337923.0
48	337923.0
49	337923.0
50	327217.5
51	316512.0
52	316512.0
53	316512.0
54	284688.0
55	252864.0
56	252864.0
57	252864.0
58	217879.5
59	182895.0
60	182895.0
61	182895.0
62	149472.5
63	116050.0
64	116050.0
65	116050.0
66	90106.5
67	64163.0
68	64163.0
69	64163.0
70	46656.0
71	29149.0
72	29149.0
73	29149.0
74	20180.5
75	11212.0
76	11212.0
77	11212.0
78	7646.0
79	4080.0
80	4080.0
81	4080.0
82	2648.0
83	1216.0
84	1216.0
85	1216.0
86	769.5
87	323.0
88	323.0
89	323.0
90	200.0
91	77.0
92	77.0
93	77.0
94	43.0
95	9.0
96	9.0
97	9.0
98	5.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07625654701254718
2	0.0
3	0.0
4	0.024132332938440613
5	0.06451539512835638
6	0.08117239261111843
7	0.030632624639030676
8	0.05821823754340977
9	0.04278004475450836
10	0.1040859208556984
11	0.003006384911522905
12	0.011050495891003109
13	0.004468950544155669
14	0.006419038054332688
15	0.005728382061144994
16	0.015275685496386651
17	0.026732449618676636
18	8.531632857024458E-4
19	0.0026813703264934015
20	0.004387696897898293
21	8.12536462573758E-5
22	2.843877619008153E-4
23	0.0
24	4.06268231286879E-5
25	0.0029657580883942166
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2461428.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.34710735023681
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.45716630815282	53.51210309100788
2	13.16716852377183	17.73541424138241
3	3.8646069311518776	7.808102935762642
4	1.4917986104006593	4.018732846383491
5	0.6912685834495473	2.32774697487114
6	0.3679247383149924	1.4867200108864547
7	0.2268812610461563	1.0695857650402814
8	0.15246127335708218	0.8214260594826569
9	0.10152846100135403	0.6153883345946268
>10	0.44384868565654956	4.978913551744224
>50	0.01931724384490051	0.9123083522939939
>100	0.012466218157104603	1.739370327672957
>500	0.002234525130939467	1.0508592501549843
>1k	0.0013286365643159807	1.9233282587220943
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	4880	0.19825889686799694	No Hit
CCCATGTACTCTGCGTTGATACCAC	4764	0.19354618538506915	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4090	0.1661637065963335	No Hit
GTCCTACAGTGGACATTTCTAAATT	3177	0.12907141707984146	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3130	0.12716195639279312	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2723	0.11062683937941714	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2627	0.10672666435906311	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2605	0.10583287425023197	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.12536462573758E-5
2	0.0	0.0	0.0	0.0	8.12536462573758E-5
3	0.0	0.0	0.0	0.0	8.12536462573758E-5
4	0.0	0.0	0.0	0.0	8.12536462573758E-5
5	0.0	0.0	0.0	0.0	8.12536462573758E-5
6	0.0	0.0	0.0	0.0	8.12536462573758E-5
7	0.0	0.0	0.0	4.06268231286879E-5	8.12536462573758E-5
8	4.06268231286879E-5	0.0	0.0	4.06268231286879E-5	8.12536462573758E-5
9	4.06268231286879E-5	0.0	0.0	4.06268231286879E-5	8.12536462573758E-5
10	4.06268231286879E-5	0.0	0.0	4.06268231286879E-5	8.12536462573758E-5
11	8.12536462573758E-5	0.0	0.0	4.06268231286879E-5	8.12536462573758E-5
12	8.12536462573758E-5	0.0	0.0	4.06268231286879E-5	8.12536462573758E-5
13	8.12536462573758E-5	0.0	0.0	4.06268231286879E-5	8.12536462573758E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	120	7.2577677E-10	12.683024	6
GGCGAGG	800	0.0	11.625306	19
GCCGGTT	90	7.535964E-6	11.601301	11
CCGGTTT	90	7.535964E-6	11.601301	12
TAGGACC	545	0.0	11.523199	4
AATCCCG	250	0.0	10.628851	19
TCCAACG	170	1.4188117E-10	10.606305	18
TGGCGAG	1625	0.0	10.453437	18
GTATTAG	155	3.614332E-9	10.428139	1
AATGCCG	210	0.0	10.393648	18
AGGACGT	1725	0.0	10.204888	5
GTCCTAC	1505	0.0	10.171358	1
GATATAC	515	0.0	10.154184	1
GGACGTG	1680	0.0	10.135096	6
TTAGACT	160	6.015398E-9	10.110079	4
AGTCGTC	160	6.1354513E-9	10.097943	8
GTAGGAC	1620	0.0	10.094512	3
GACGTGA	955	0.0	9.952182	7
CCAACGA	105	4.15443E-5	9.941952	19
TATACTG	345	0.0	9.929081	5
>>END_MODULE