FastQCFastQC Report
Thu 26 May 2016
SRR1512985_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512985_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2716810
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT89570.32968812688410304No Hit
CTGTAGGACGTGGAATATGGCAAGA80150.2950151096322525No Hit
GTCCTAAAGTGTGTATTTCTCATTT72370.26637858370662654No Hit
GTACATGGGAAGCAGTGGTATCAAC69170.2546000640457007No Hit
CTTTAGGACGTGAAATATGGCGAGG63520.23380361526937843No Hit
CCCATGTACTCTGCGTTGATACCAC58230.21433224995491035No Hit
CTGAAGGACCTGGAATATGGCGAGA37760.1389865319989252No Hit
GTCCTACAGTGTGCATTTCTCATTT33970.12503634777551614No Hit
GTATCAACGCAGAGTACTTTTTTTT32690.12032493991114579No Hit
GTCCTTCAGTGTGCATTTCTCATTT31810.11708584700439119No Hit
GAGTACATGGGAAGCAGTGGTATCA29310.10788387851929285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG400.00530266514.2409857
GGCGAGG15700.011.79211419
TAGGACC10300.011.7109254
CGAAATT658.068293E-411.68512419
GCGCCAC1157.0456736E-811.56940213
AGGACCT22700.010.8785795
GTCCTAC38650.010.4994471
CGTACAC1002.4062268E-510.4476193
GGACCTG22650.010.39518456
GTCCTAA20350.010.3450871
AGGACGT43800.010.2351115
GGACGTG43400.010.1940076
TAGACTG3750.010.1310255
AACAGCG750.002662602610.1269227
ATTTAGA9100.09.9446281
TGTAGGA40650.09.9067222
GACGTGG23150.09.8835927
GTAGGAC39700.09.880623
CTAACAC2500.09.8777493
CTGTAGG39850.09.8486161
Produced by FastQC (version 0.11.5)