##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512985_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2716810
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.091124517356754	31.0	30.0	33.0	24.0	34.0
2	29.200908786407588	31.0	30.0	34.0	23.0	34.0
3	29.35862574121856	31.0	30.0	34.0	24.0	34.0
4	32.865292015267904	35.0	33.0	37.0	26.0	37.0
5	32.96994894747885	35.0	35.0	37.0	26.0	37.0
6	33.106239302711636	37.0	35.0	37.0	26.0	37.0
7	33.022980259937206	37.0	35.0	37.0	26.0	37.0
8	33.03247411486265	37.0	35.0	37.0	26.0	37.0
9	34.392730812975515	39.0	35.0	39.0	26.0	39.0
10	34.23472197172419	39.0	35.0	39.0	25.0	39.0
11	34.32044382934397	39.0	35.0	39.0	26.0	39.0
12	34.20757542853567	39.0	35.0	39.0	25.0	39.0
13	34.22212447686809	39.0	35.0	39.0	25.0	39.0
14	35.23592264457213	39.0	35.0	41.0	25.0	41.0
15	35.183781714584384	39.0	35.0	41.0	25.0	41.0
16	35.07400480710834	39.0	35.0	41.0	25.0	41.0
17	35.034453642323165	39.0	35.0	41.0	25.0	41.0
18	34.72017329147051	39.0	34.0	41.0	19.0	41.0
19	34.955527990547736	39.0	34.0	41.0	23.0	41.0
20	35.03749838965552	40.0	35.0	41.0	24.0	41.0
21	34.97360176088869	39.0	35.0	41.0	24.0	41.0
22	34.90291003051373	39.0	35.0	41.0	23.0	41.0
23	34.638297856677504	39.0	34.0	41.0	18.0	41.0
24	34.64766840522525	39.0	35.0	41.0	19.0	41.0
25	34.56859993889893	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6446490107775311
1101	2	0.8462740730818936
1101	3	0.760698440339759
1101	4	0.8744637602397347
1101	5	0.6173965540319486
1101	6	0.863148310901451
1101	7	0.877642035838079
1101	8	0.6490475810141518
1101	9	0.6453888399700745
1101	10	0.7752397194527987
1101	11	0.9478473836068204
1101	12	1.14999611635033
1101	13	0.934683188407945
1101	14	0.9505456332689519
1101	15	0.7554807890458903
1101	16	1.1704114070369158
1101	17	1.1573959224339205
1101	18	1.3335810766903364
1101	19	0.9237115604299291
1101	20	1.1386183515507824
1101	21	0.9908671057811631
1101	22	1.1004253386759757
1101	23	1.334204218818492
1101	24	0.9244294874820937
1101	25	1.2277682819370739
1102	1	0.45645395312384096
1102	2	0.5116055452480097
1102	3	0.6084866766245653
1102	4	0.6809482945015475
1102	5	0.5632945984028908
1102	6	0.5832863429217952
1102	7	0.7893924764715621
1102	8	0.7357389878717768
1102	9	0.744852945446091
1102	10	0.9031055914392709
1102	11	0.8990396962922702
1102	12	0.9338271774144715
1102	13	0.87412892002326
1102	14	0.8640183748103638
1102	15	1.0374875631643548
1102	16	1.1153633320818557
1102	17	0.9372821588801301
1102	18	1.2417977946814034
1102	19	1.2888428616056942
1102	20	1.1724936806370394
1102	21	0.8857399682555638
1102	22	1.2168912300772163
1102	23	1.450717477588178
1102	24	1.3192566199785674
1102	25	1.2101517143520226
1103	1	0.588934051144939
1103	2	0.7725459864798445
1103	3	0.7665300651061742
1103	4	0.9309860563531771
1103	5	0.41420350677735485
1103	6	0.8225746403984786
1103	7	0.7493314870882344
1103	8	0.8312477232946804
1103	9	1.0871067461593427
1103	10	1.0548563267115725
1103	11	1.0628976113360906
1103	12	1.186218609238928
1103	13	1.1516918773580755
1103	14	1.3528259217046497
1103	15	1.4595850914172956
1103	16	1.5130185606722577
1103	17	1.3225629085498554
1103	18	1.6444843621861267
1103	19	1.6395753958936794
1103	20	1.5750730090464984
1103	21	1.3521659173785991
1103	22	1.4447541146263418
1103	23	1.738070679816012
1103	24	1.5700491625262458
1103	25	1.5424001074768583
1104	1	0.6491084773181299
1104	2	0.6524637472408195
1104	3	0.6333124951727847
1104	4	0.9149959197419975
1104	5	0.6811037966310565
1104	6	0.7784332366057996
1104	7	0.9737155803788156
1104	8	0.9401780845822074
1104	9	1.0547599996676666
1104	10	1.0605923688025243
1104	11	1.0391657198662827
1104	12	0.9573780589801331
1104	13	1.0369079185067704
1104	14	1.169754969256232
1104	15	1.4147224064660762
1104	16	1.1172215125882374
1104	17	1.243630551186179
1104	18	1.533256090083647
1104	19	1.2458200147440124
1104	20	1.2797007385904635
1104	21	1.3629272586921815
1104	22	1.313347721750091
1104	23	1.6161482409558516
1104	24	1.402688772048215
1104	25	1.2447929305292718
1105	1	0.705416687605755
1105	2	0.8459848500296552
1105	3	0.8453727252115542
1105	4	0.9516418769711521
1105	5	0.766370518251172
1105	6	0.6004838080858548
1105	7	0.916014176914608
1105	8	0.9909918227520578
1105	9	0.9146437436351107
1105	10	1.0441605583886968
1105	11	1.0062992010809069
1105	12	0.9695719406000052
1105	13	0.9085723064684146
1105	14	1.0894131596008947
1105	15	1.1985664704817367
1105	16	1.217996264867189
1105	17	1.141191535223392
1105	18	1.154973853410894
1105	19	1.2079203564188035
1105	20	1.2724108144718187
1105	21	1.2824728187952346
1105	22	0.992686581674576
1105	23	1.1414359593942862
1105	24	1.2422912703329914
1105	25	1.2082727000814444
1106	1	0.5457639506055827
1106	2	0.7026489432166159
1106	3	0.6950643648643045
1106	4	0.8262139706536473
1106	5	0.7181590322146931
1106	6	0.6833954674076494
1106	7	0.551488506019119
1106	8	0.6571819493424442
1106	9	0.8586026306639241
1106	10	0.963375884396541
1106	11	0.8519063125937905
1106	12	0.8890525614487217
1106	13	0.7502180663340141
1106	14	1.0913456643260204
1106	15	1.0669050062937089
1106	16	1.1508539725586502
1106	17	1.0419493291982675
1106	18	1.3633163038730416
1106	19	0.9370716872583387
1106	20	0.991737364008145
1106	21	0.9466314313630946
1106	22	1.0223894253261037
1106	23	1.3405772945948087
1106	24	1.2065939825236853
1106	25	1.2850488855095819
1107	1	0.3542766168026006
1107	2	0.3821857644957838
1107	3	0.6102885057082084
1107	4	0.5853150802807221
1107	5	0.513146212478361
1107	6	0.430448840650584
1107	7	0.6959911076871208
1107	8	0.6029426702653353
1107	9	0.7239759543492497
1107	10	0.6204001223580491
1107	11	0.6786108912532072
1107	12	0.8780552677784712
1107	13	0.7073582822666182
1107	14	1.0285541388464594
1107	15	1.1000670556239314
1107	16	0.9997525271639205
1107	17	1.0452738008158065
1107	18	1.2975454780440074
1107	19	1.17245984422334
1107	20	1.0278598001936956
1107	21	1.0569288726223505
1107	22	0.988161898116708
1107	23	1.116791609984766
1107	24	1.06671588955642
1107	25	1.0432145209657975
1108	1	0.45717296257566886
1108	2	0.4153002916682027
1108	3	0.3871188389644047
1108	4	0.5355272723456892
1108	5	0.5327113365423912
1108	6	0.6639477532513212
1108	7	0.6322137015086824
1108	8	0.6995656071124117
1108	9	0.7169881183522975
1108	10	0.7261199890430063
1108	11	0.5978818363848291
1108	12	0.6434074395927922
1108	13	0.6835237156399856
1108	14	0.8861929484767259
1108	15	0.8175122485024886
1108	16	0.7626638358020728
1108	17	0.7253585310496931
1108	18	0.9095374971512413
1108	19	0.9399543548727323
1108	20	0.8269884148669391
1108	21	0.7526178607284919
1108	22	0.6935241847859359
1108	23	0.9227442439830327
1108	24	0.9663703510346338
1108	25	1.0598389027921442
1109	1	0.11824962599379418
1109	2	0.04373983633914236
1109	3	0.20486371995736619
1109	4	0.2920920688708648
1109	5	0.2652023240151209
1109	6	0.16785110842283046
1109	7	0.21287988745051933
1109	8	0.2772673823564702
1109	9	0.6242111329222624
1109	10	0.6413591184561582
1109	11	0.24312525489548875
1109	12	0.47147677582039904
1109	13	0.49825346953679883
1109	14	0.2466649597232191
1109	15	0.4793535918724885
1109	16	0.5557004743064269
1109	17	0.35540186220480763
1109	18	-1.1823353648123316
1109	19	0.2207403782414019
1109	20	0.43581209000469556
1109	21	0.5476912357676511
1109	22	0.6916523031267516
1109	23	0.24437376717871473
1109	24	0.6555095565968116
1109	25	0.7823402483183912
1110	1	0.25908069905202424
1110	2	0.2942308412704371
1110	3	0.319341637718761
1110	4	0.18133976905569682
1110	5	0.3390932548235597
1110	6	0.26705948100115506
1110	7	0.3294557890229157
1110	8	0.25704293644845677
1110	9	0.5892152285643562
1110	10	0.691540176524569
1110	11	0.5941849285188425
1110	12	0.10612189492449886
1110	13	0.2893432644242111
1110	14	0.4359119517278671
1110	15	0.4713186974739614
1110	16	0.6162622736506549
1110	17	0.256590070532539
1110	18	0.7259358257093851
1110	19	0.5248246894535455
1110	20	0.4795498886501832
1110	21	0.5447245509243572
1110	22	0.5826202910215912
1110	23	0.5304767932326655
1110	24	0.7424333634902354
1110	25	0.7786476787809988
1111	1	-0.017593027175038856
1111	2	0.3107942685012297
1111	3	0.2786051875629738
1111	4	0.15197027272613894
1111	5	0.09233507926875006
1111	6	0.22896306757630924
1111	7	0.19137503402151168
1111	8	-0.02609320690839212
1111	9	0.3005309607681568
1111	10	0.311129395036545
1111	11	0.1359278968251303
1111	12	0.25076857188857105
1111	13	0.2644954696079438
1111	14	0.18985707824261766
1111	15	0.3538281057978736
1111	16	0.3365743009122326
1111	17	0.25984118387298594
1111	18	0.5043857214083616
1111	19	0.15325892672908736
1111	20	0.09233101043025016
1111	21	0.2457156760484338
1111	22	0.07182526774627007
1111	23	0.3771821547830072
1111	24	0.43832024066981035
1111	25	-0.025373218579360923
1112	1	-0.22190595261723445
1112	2	0.11403679638623743
1112	3	0.0920468273946966
1112	4	0.2778843275694314
1112	5	0.19058664102912104
1112	6	0.08470574547031617
1112	7	-0.18130199326679275
1112	8	0.10377925210581651
1112	9	0.3101178693160094
1112	10	0.2776747210894399
1112	11	0.24557314220652415
1112	12	0.21842263113212823
1112	13	-0.038668053536774494
1112	14	0.15734712764558623
1112	15	0.21115604547425448
1112	16	0.28013074763237
1112	17	0.3597975145861625
1112	18	0.28738120113714416
1112	19	0.19944919814537343
1112	20	0.02656756462350529
1112	21	-0.0742943578869415
1112	22	0.02614852714183513
1112	23	0.10624056495428391
1112	24	-0.09778559234683826
1112	25	0.26302225181552785
1113	1	-0.35439793317991786
1113	2	-0.10236112346101578
1113	3	-0.28025593607184973
1113	4	0.008700047680662237
1113	5	-0.218513557291665
1113	6	-0.11925583642327808
1113	7	-0.2905811771035829
1113	8	0.0857442794370229
1113	9	-0.18041845615029217
1113	10	-0.38276008078447177
1113	11	-0.23526412867389013
1113	12	-0.015201402607921466
1113	13	-0.16996903520452378
1113	14	-0.22346261733027006
1113	15	-0.576334840765881
1113	16	-0.5699336361297611
1113	17	-0.4456920906923685
1113	18	-0.48040811961752183
1113	19	-0.4796951624681469
1113	20	-0.5898373374044183
1113	21	-0.40180535817830787
1113	22	-0.27601500895671194
1113	23	-0.7252791469502
1113	24	-0.1275960127143918
1113	25	-0.2868593867948519
1114	1	-1.4533413561958817
1114	2	-1.2725488862431362
1114	3	-1.0872729537260604
1114	4	-1.4537409224324414
1114	5	-1.397501992450767
1114	6	-1.7731909464343794
1114	7	-1.8751141329621213
1114	8	-1.4795950756550553
1114	9	-1.528414433972209
1114	10	-1.8172672506837344
1114	11	-2.305548993394705
1114	12	-1.9781939656783223
1114	13	-1.8412909486305935
1114	14	-2.1170155880561907
1114	15	-1.989262129755275
1114	16	-2.689016055176417
1114	17	-4.794083273404773
1114	18	-3.820661242793488
1114	19	-2.71588661943958
1114	20	-1.8986567288773557
1114	21	-2.821100052852053
1114	22	-3.0294124979005765
1114	23	-2.8602571533610863
1114	24	-2.393671130774166
1114	25	-2.2137333732933726
1115	1	-1.0066750453171025
1115	2	-0.7112852491537254
1115	3	-0.8148559304037306
1115	4	-0.8846521212467131
1115	5	-0.7068113994825254
1115	6	-0.8963332563829027
1115	7	-1.8909421381548874
1115	8	-1.3676275426014932
1115	9	-1.058385007710548
1115	10	-0.9354324351901226
1115	11	-1.6993376310915593
1115	12	-1.3406724014612124
1115	13	-1.07692926681257
1115	14	-1.1217458314151045
1115	15	-2.2686673478619284
1115	16	-1.2263221507774844
1115	17	-1.2559059313659162
1115	18	-1.1978790596095905
1115	19	-1.2731647813525697
1115	20	-1.1123774284431178
1115	21	-1.828353136057821
1115	22	-1.9586297992581265
1115	23	-1.278571942335482
1115	24	-1.3304591211106214
1115	25	-1.6981652668138665
1116	1	-1.1417110783290205
1116	2	-2.000581274394662
1116	3	-1.540523503275132
1116	4	-1.2487939564281128
1116	5	-1.549093965677276
1116	6	-1.4298187202195543
1116	7	-1.1753879085026888
1116	8	-1.862199689777995
1116	9	-1.7953334155198917
1116	10	-2.836284630369658
1116	11	-1.4932215483551943
1116	12	-1.3948780767374416
1116	13	-2.0319848317949507
1116	14	-1.7133837872533064
1116	15	-1.424026897194146
1116	16	-1.17574467580571
1116	17	-1.660856797117276
1116	18	-1.489192988849382
1116	19	-1.8416295020767492
1116	20	-2.056823666033523
1116	21	-2.3519788587468824
1116	22	-1.527323684036098
1116	23	-1.5933531313941316
1116	24	-2.2399700595059144
1116	25	-2.0008673123528027
1201	1	0.26447963189850654
1201	2	0.2618936174996449
1201	3	0.44665566013767943
1201	4	0.6109349407837357
1201	5	0.6741306921337227
1201	6	0.6371516072121608
1201	7	0.5816923830427285
1201	8	0.23362763768350447
1201	9	0.3678956584685622
1201	10	0.700145595969154
1201	11	0.2707413751801866
1201	12	-0.2567490142750799
1201	13	0.10440009958593777
1201	14	0.7299788987668379
1201	15	0.3553173716824247
1201	16	0.8751726523162802
1201	17	0.9869164651945965
1201	18	1.2258502469524757
1201	19	0.8659674239529238
1201	20	0.813487706853735
1201	21	0.585345255006601
1201	22	0.863068669372062
1201	23	1.127849117877716
1201	24	0.9621812729234591
1201	25	0.8564645782762241
1202	1	0.6965470640287847
1202	2	0.8034610918851186
1202	3	0.5034470011170562
1202	4	0.9043072133541017
1202	5	0.8284865940386439
1202	6	0.8415741863833546
1202	7	0.8752204268850008
1202	8	0.9544577660676836
1202	9	1.2141234512147037
1202	10	1.3060227923440877
1202	11	0.9742586904999158
1202	12	1.138655589965623
1202	13	1.1346356615585549
1202	14	1.3550118434956104
1202	15	1.010503874656763
1202	16	1.116771925526912
1202	17	1.3659831696760847
1202	18	1.7082620589268842
1202	19	1.4974115203291518
1202	20	1.3138730169424164
1202	21	1.3469681195077925
1202	22	1.4165282956069518
1202	23	1.4139085558979332
1202	24	1.5223832885679087
1202	25	1.287112701357728
1203	1	0.31692950298233313
1203	2	0.5057376382004293
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2211	17	0.461563749074287
2211	18	0.4017987328557169
2211	19	0.33432781295061886
2211	20	0.2162508653002604
2211	21	0.2616653980919921
2211	22	0.24899979380016646
2211	23	0.7979457941756678
2211	24	0.637498797808945
2211	25	0.5746827345770242
2212	1	-0.2320644584926299
2212	2	-0.2680439846091147
2212	3	-0.2150065903966052
2212	4	-0.368047586891457
2212	5	-0.3498549703090177
2212	6	-0.3992001411643997
2212	7	-0.4385169141744214
2212	8	-0.4774649775363642
2212	9	-0.35694325566626617
2212	10	-0.15987856691062774
2212	11	-0.49720471139431766
2212	12	-0.17792528431356658
2212	13	-0.3347727107674885
2212	14	-0.3588909603287931
2212	15	-0.49946944124288706
2212	16	-0.3075471131939551
2212	17	-0.3047004341770787
2212	18	-3.14102006519715
2212	19	-1.2554993269177146
2212	20	-0.7344618805925478
2212	21	-0.7552484974245601
2212	22	-0.47231324599378866
2212	23	-0.4954092556126781
2212	24	-0.9919613109487315
2212	25	-0.5647825100510033
2213	1	-0.23399818257303906
2213	2	-0.09423162928023388
2213	3	-0.4195231893389817
2213	4	-0.5025285195853684
2213	5	-0.4900337319215211
2213	6	-0.4637160645890077
2213	7	-0.37195068537764797
2213	8	-0.6662076918865907
2213	9	-0.5608428453981773
2213	10	-0.5948489231209493
2213	11	-0.6742607504958968
2213	12	-0.7058292888809348
2213	13	-0.5325590281551484
2213	14	-0.6427463036470726
2213	15	-0.6437440196026714
2213	16	-1.1982328127552506
2213	17	-0.7631416551427535
2213	18	-0.6199314901092663
2213	19	-0.5688902136382907
2213	20	-1.2000283975739237
2213	21	-0.8242012651134587
2213	22	-0.8121246675827933
2213	23	-1.3252261194778683
2213	24	-0.965869164877823
2213	25	-1.1372381642164129
2214	1	-1.7314083507499376
2214	2	-3.7418374480269883
2214	3	-1.9498521841615215
2214	4	-1.6540110934737058
2214	5	-1.6297018632405198
2214	6	-1.7226278348174802
2214	7	-1.8484609756095516
2214	8	-1.5052754983819767
2214	9	-1.7866497584815093
2214	10	-1.7688192367769418
2214	11	-1.4129930765848897
2214	12	-2.0952223997058823
2214	13	-1.915188146469042
2214	14	-3.1052785331994457
2214	15	-3.3521259793702747
2214	16	-2.5627062925785324
2214	17	-2.9321818709751994
2214	18	-2.3911097477540935
2214	19	-4.5778937538936795
2214	20	-2.919948186689936
2214	21	-2.720750874269868
2214	22	-2.8931019717460167
2214	23	-2.8685804926367524
2214	24	-3.059484206613636
2214	25	-2.799233546350976
2215	1	-0.9307093161951592
2215	2	-2.8459887830384574
2215	3	-1.4119727846063448
2215	4	-1.0643139894773945
2215	5	-0.9120981175089256
2215	6	-1.4251753467944006
2215	7	-1.8854573192506159
2215	8	-1.7871728995678637
2215	9	-1.4483326961173404
2215	10	-3.1148916120301777
2215	11	-2.10221655766766
2215	12	-2.4434870608586685
2215	13	-1.6749643923360935
2215	14	-2.996285092450563
2215	15	-2.717932899574059
2215	16	-2.4419469735701966
2215	17	-2.8044090866683646
2215	18	-1.6400040012155657
2215	19	-2.310025417435625
2215	20	-2.4635302209600454
2215	21	-2.1748158421920962
2215	22	-1.7954080423878835
2215	23	-2.402408956179926
2215	24	-2.1813725993961484
2215	25	-1.9594425702386573
2216	1	-1.842725442115043
2216	2	-1.9862266121231542
2216	3	-2.4588979955981713
2216	4	-3.357664893651645
2216	5	-2.2784543629501464
2216	6	-2.22800561376895
2216	7	-2.2504103109327573
2216	8	-2.6909093046220818
2216	9	-3.1369004130157343
2216	10	-2.6806632974294757
2216	11	-3.291471841585196
2216	12	-3.6283260686264285
2216	13	-4.301783650041493
2216	14	-3.608701531626412
2216	15	-3.454262531464142
2216	16	-2.5877872870265293
2216	17	-2.714162087367029
2216	18	-2.7770078916415493
2216	19	-3.0529580037634503
2216	20	-2.869606482781286
2216	21	-3.2529214974322542
2216	22	-3.8541720542910625
2216	23	-3.8923715819971783
2216	24	-3.62449710566451
2216	25	-3.8250568876620363
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	115197.0
3	8.0
4	885.0
5	5378.0
6	7066.0
7	6206.0
8	5627.0
9	5367.0
10	5039.0
11	4800.0
12	4636.0
13	4585.0
14	4719.0
15	4676.0
16	4903.0
17	5288.0
18	5965.0
19	7088.0
20	9229.0
21	11552.0
22	14804.0
23	17908.0
24	21063.0
25	24025.0
26	26853.0
27	30140.0
28	34603.0
29	41295.0
30	51028.0
31	63876.0
32	81152.0
33	104291.0
34	135426.0
35	179896.0
36	249843.0
37	401387.0
38	1021006.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.69387510864157	19.065473791756133	12.73310612023727	25.507544979365026
2	14.074617951405024	19.957882783197594	42.65555716754905	23.31194209784833
3	18.892418789577906	24.69631930529198	29.48653936931623	26.92472253581389
4	12.03101895218065	16.97260711781929	38.717944186210914	32.27842974378914
5	12.641909409959537	38.43903423353361	36.41346393546632	12.505592421040538
6	31.651785155499333	37.347598575439214	18.360878916014382	12.639737353047071
7	27.752320850787804	31.44856357034598	22.598461981929447	18.200653596936775
8	24.375281185134135	38.303876544355276	20.68804667728451	16.632795593226078
9	26.362015824653533	15.186240827488303	20.529528014172385	37.92221533368578
10	14.827779168568778	29.11908934277762	35.15742117896535	20.89571030968825
11	33.668541505756686	22.284066469291933	25.6518925223151	18.395499502636287
12	23.795202789690485	24.505326572633894	32.34698689792352	19.352483739752095
13	29.071533704477364	22.276557034126746	25.749644919570326	22.902264341825564
14	20.042281398835385	23.204844614754748	27.723254916630097	29.029619069779773
15	22.52553431434093	32.51076655040719	22.98366267790995	21.980036457341928
16	21.136186839188134	28.29345191791216	29.397579982188756	21.172781260710956
17	21.20630876733107	29.45788632732253	28.502367260383917	20.833437644962487
18	21.160889706413393	27.48897889519228	31.165959930490768	20.184171467903568
19	23.3741800948984	27.23896312746487	27.651014222978844	21.735842554657886
20	23.490146615301242	27.859116184821282	28.4202996512806	20.230437548596875
21	22.117039967129728	27.256407273284218	27.455072139351106	23.171480620234952
22	22.698133664177362	29.327040424697703	27.031957250435795	20.94286866068914
23	21.17855739909043	28.933896714513814	28.29035508550673	21.59719080088903
24	23.00840256936611	27.390483861942222	28.824082064210593	20.777031504481076
25	22.144806801790427	27.886081644163628	28.37738833219327	21.591723221852675
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	185.0
1	185.0
2	362.0
3	539.0
4	539.0
5	539.0
6	1118.0
7	1697.0
8	1697.0
9	1697.0
10	2687.5
11	3678.0
12	3678.0
13	3678.0
14	6939.0
15	10200.0
16	10200.0
17	10200.0
18	20008.0
19	29816.0
20	29816.0
21	29816.0
22	49808.0
23	69800.0
24	69800.0
25	69800.0
26	103978.5
27	138157.0
28	138157.0
29	138157.0
30	182130.5
31	226104.0
32	226104.0
33	226104.0
34	255921.0
35	285738.0
36	285738.0
37	285738.0
38	302657.0
39	319576.0
40	319576.0
41	319576.0
42	333284.0
43	346992.0
44	346992.0
45	346992.0
46	359728.0
47	372464.0
48	372464.0
49	372464.0
50	348567.0
51	324670.0
52	324670.0
53	324670.0
54	279028.5
55	233387.0
56	233387.0
57	233387.0
58	197712.5
59	162038.0
60	162038.0
61	162038.0
62	130836.5
63	99635.0
64	99635.0
65	99635.0
66	76286.0
67	52937.0
68	52937.0
69	52937.0
70	38594.5
71	24252.0
72	24252.0
73	24252.0
74	16963.0
75	9674.0
76	9674.0
77	9674.0
78	6616.0
79	3558.0
80	3558.0
81	3558.0
82	2376.5
83	1195.0
84	1195.0
85	1195.0
86	786.0
87	377.0
88	377.0
89	377.0
90	240.0
91	103.0
92	103.0
93	103.0
94	66.0
95	29.0
96	29.0
97	29.0
98	19.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3501533047949617
2	0.03886911488105536
3	0.03548279047853917
4	0.03393685977304265
5	0.03978931172956519
6	0.017078853508342505
7	0.016821198390759753
8	0.023115344834567012
9	0.019949867675693185
10	0.019508173188408463
11	0.017962242482911943
12	0.01925051807082571
13	0.019287325944766103
14	0.026575284984963983
15	0.02793717632075854
16	0.020207522793275937
17	0.021716645624832064
18	0.10769983914959089
19	0.02363065506973252
20	0.021569414129070492
21	0.024955738531586676
22	0.0193241338187065
23	0.021753453498772456
24	0.018330321222315878
25	0.021495798381189705
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2716810.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.08098025002077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.00060248232691	53.525992128744285
2	12.524479568339416	16.552597739944513
3	3.4493595369038066	6.838111783000837
4	1.2969993434468112	3.4282795199439455
5	0.566474971573331	1.8716610704334182
6	0.31097025102562076	1.2329531409820829
7	0.18056983420685202	0.835256215358076
8	0.12540231993777262	0.662936658169179
9	0.08752672506363487	0.5205466611251148
>10	0.4058857352993441	4.736393427391841
>50	0.027112964771226947	1.2357166054145279
>100	0.018864065045674977	2.6985732419433397
>500	0.0030157175846665953	1.3619788473263914
>1k	0.0024014047432228025	2.9010230635716683
>5k	3.350797316124841E-4	1.5979798966507672
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	8957	0.32968812688410304	No Hit
CTGTAGGACGTGGAATATGGCAAGA	8015	0.2950151096322525	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	7237	0.26637858370662654	No Hit
GTACATGGGAAGCAGTGGTATCAAC	6917	0.2546000640457007	No Hit
CTTTAGGACGTGAAATATGGCGAGG	6352	0.23380361526937843	No Hit
CCCATGTACTCTGCGTTGATACCAC	5823	0.21433224995491035	No Hit
CTGAAGGACCTGGAATATGGCGAGA	3776	0.1389865319989252	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3397	0.12503634777551614	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3269	0.12032493991114579	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	3181	0.11708584700439119	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2931	0.10788387851929285	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	7.361574788078666E-5	0.0	0.0	0.0
6	0.0	7.361574788078666E-5	0.0	0.0	0.0
7	0.0	7.361574788078666E-5	0.0	0.0	0.0
8	0.0	7.361574788078666E-5	0.0	0.0	0.0
9	0.0	7.361574788078666E-5	0.0	0.0	0.0
10	0.0	7.361574788078666E-5	0.0	0.0	0.0
11	0.0	7.361574788078666E-5	0.0	3.680787394039333E-5	0.0
12	0.0	1.1042362182117999E-4	0.0	3.680787394039333E-5	0.0
13	0.0	1.4723149576157331E-4	0.0	3.680787394039333E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGCG	40	0.005302665	14.240985	7
GGCGAGG	1570	0.0	11.792114	19
TAGGACC	1030	0.0	11.710925	4
CGAAATT	65	8.068293E-4	11.685124	19
GCGCCAC	115	7.0456736E-8	11.569402	13
AGGACCT	2270	0.0	10.878579	5
GTCCTAC	3865	0.0	10.499447	1
CGTACAC	100	2.4062268E-5	10.447619	3
GGACCTG	2265	0.0	10.3951845	6
GTCCTAA	2035	0.0	10.345087	1
AGGACGT	4380	0.0	10.235111	5
GGACGTG	4340	0.0	10.194007	6
TAGACTG	375	0.0	10.131025	5
AACAGCG	75	0.0026626026	10.126922	7
ATTTAGA	910	0.0	9.944628	1
TGTAGGA	4065	0.0	9.906722	2
GACGTGG	2315	0.0	9.883592	7
GTAGGAC	3970	0.0	9.88062	3
CTAACAC	250	0.0	9.877749	3
CTGTAGG	3985	0.0	9.848616	1
>>END_MODULE