FastQCFastQC Report
Thu 26 May 2016
SRR1512985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512985_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2716810
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT82870.3050268513440395No Hit
CTGTAGGACGTGGAATATGGCAAGA80010.29449979939708704No Hit
GTACATGGGAAGCAGTGGTATCAAC68090.25062481366013817No Hit
CTTTAGGACGTGAAATATGGCGAGG67780.24948376956798599No Hit
GTCCTAAAGTGTGTATTTCTCATTT66600.24514044044301958No Hit
CCCATGTACTCTGCGTTGATACCAC66470.24466193808179446No Hit
GTATCAACGCAGAGTACTTTTTTTT39000.14355070836753397No Hit
CTGAAGGACCTGGAATATGGCGAGA38080.1401643839650178No Hit
GTCCTACAGTGTGCATTTCTCATTT33120.1219076784905827No Hit
TATCAACGCAGAGTACTTTTTTTTT28570.10516009584770375No Hit
TTTCTAAATTTTCCACCTTTTTCAG28080.10335651002462447No Hit
ATTTAGAAATGTCCACTGTAGGACG27780.10225227380641266No Hit
GTCCTTCAGTGTGCATTTCTCATTT27590.1015529242015452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10800.013.2125454
GCCGGTT1850.011.80219411
GGCGAGG19600.011.71844619
CTATACG752.0486489E-411.415644
GTACCGT600.005831492611.0950596
AGGACCT24900.011.0794365
TGTACCG1053.412204E-610.8720395
GTCGAGG1408.485586E-910.84682619
TGTAGGA38550.010.7747622
GGACCTG24500.010.6745476
TCCAACG2500.010.63008518
GGACGTG42450.010.4621766
AGGACGT42400.010.4553615
TGGCGAG42100.010.41546318
AAATGTC9050.010.3976987
ATAGGAC4400.010.3696293
GTAGGAC38150.010.3650983
CTGTAGG39100.010.358661
TTAGGAC28100.010.2835233
GTCCTAG3700.010.2784821
Produced by FastQC (version 0.11.5)