FastQCFastQC Report
Thu 26 May 2016
SRR1512984_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512984_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2591520
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT71560.27613138235475704No Hit
CTGTAGGACGTGGAATATGGCAAGA65230.25170556275853556No Hit
GTCCTAAAGTGTGTATTTCTCATTT57100.22033401247144535No Hit
CTTTAGGACGTGAAATATGGCGAGG53370.20594091498425637No Hit
GTACATGGGAAGCAGTGGTATCAAC52620.20304686052972773No Hit
CCCATGTACTCTGCGTTGATACCAC44490.17167531024263752No Hit
GTCCTACAGTGTGCATTTCTCATTT34110.1316215965919615No Hit
GTATCAACGCAGAGTACTTTTTTTT32060.1237111810829166No Hit
CTGAAGGACCTGGAATATGGCGAGA29720.1146817311847873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG400.005300006414.2420258
GGACGTA853.970954E-612.2872376
GTACCGT701.09716035E-412.2074516
GACCGTC701.09716035E-412.2074517
TAGGACC10550.011.8828144
GCGTTTC802.8601637E-511.87821213
GGCGTGC1459.822543E-1111.7865048
TCCAACG2250.011.40286318
AGGACGT34600.011.3907695
GGACGTG33750.011.3373556
GGCGAGG15600.011.26015619
GACGTGG17200.011.0403297
GCGCCAC1502.1263986E-910.76957813
GTCCTAA17250.010.7163391
TTAGGAC21150.010.6425133
ATAGGAC3950.010.5794183
GACGTGA18850.010.5776317
TGTAGGA34250.010.5650272
TAGGACG37700.010.5301074
GTCCTAC32400.010.5286281
Produced by FastQC (version 0.11.5)