##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512984_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2591520
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.194597379144287	31.0	30.0	34.0	25.0	34.0
2	29.31092910724208	31.0	30.0	34.0	23.0	34.0
3	29.468702923380874	31.0	30.0	34.0	25.0	34.0
4	32.96673265110823	35.0	33.0	37.0	26.0	37.0
5	33.07429886707415	35.0	35.0	37.0	27.0	37.0
6	33.18889879298636	37.0	35.0	37.0	27.0	37.0
7	33.106284728653456	37.0	35.0	37.0	26.0	37.0
8	33.10969238130518	37.0	35.0	37.0	26.0	37.0
9	34.48380988763351	39.0	35.0	39.0	27.0	39.0
10	34.34151617583503	39.0	35.0	39.0	26.0	39.0
11	34.421562249181946	39.0	35.0	39.0	27.0	39.0
12	34.30928721368154	39.0	35.0	39.0	26.0	39.0
13	34.320028786194975	39.0	35.0	39.0	26.0	39.0
14	35.35562681360746	40.0	36.0	41.0	25.0	41.0
15	35.30373873248132	40.0	36.0	41.0	25.0	41.0
16	35.194373958140396	40.0	35.0	41.0	25.0	41.0
17	35.15575646724702	40.0	35.0	41.0	25.0	41.0
18	34.84856416311663	39.0	34.0	41.0	22.0	41.0
19	35.07996696919182	40.0	35.0	41.0	24.0	41.0
20	35.16521346545657	40.0	35.0	41.0	25.0	41.0
21	35.09704189047354	40.0	35.0	41.0	24.0	41.0
22	35.02558614249553	40.0	35.0	41.0	24.0	41.0
23	34.76545116379577	39.0	35.0	41.0	19.0	41.0
24	34.773620886583934	40.0	35.0	41.0	19.0	41.0
25	34.700701518799775	39.0	35.0	41.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5891335764467804
1101	2	0.9052888796719145
1101	3	0.8177892310037578
1101	4	0.9189361422786249
1101	5	0.6580420992648754
1101	6	0.840701566010928
1101	7	0.797829671618338
1101	8	0.698540226132117
1101	9	0.5818739909580088
1101	10	0.748505398492334
1101	11	0.8242540792395019
1101	12	1.039260941233529
1101	13	0.8955687314201413
1101	14	0.8260382357347353
1101	15	0.6234725876867984
1101	16	1.122989387607305
1101	17	1.1605860867495181
1101	18	1.29097366091964
1101	19	0.8049049427523158
1101	20	1.0020462794585754
1101	21	0.9581617414173422
1101	22	0.9932739755251419
1101	23	1.2830425468357518
1101	24	0.8796772036056808
1101	25	1.0922007994205032
1102	1	0.6280360713312163
1102	2	0.7434929657315301
1102	3	0.7215062340435594
1102	4	0.8926917747250016
1102	5	0.6779527548414492
1102	6	0.7380008001047429
1102	7	0.9629787613723977
1102	8	0.8706931594588667
1102	9	0.888821957951123
1102	10	1.119472818669685
1102	11	0.9801691042290201
1102	12	1.0267339350270603
1102	13	0.876301092287946
1102	14	1.0449636429219922
1102	15	1.1110585935572317
1102	16	1.0937553809466607
1102	17	0.9348836348619614
1102	18	1.1754224437882854
1102	19	1.1870277298412404
1102	20	1.1206713863455349
1102	21	0.9976078950847125
1102	22	1.2372320903636194
1102	23	1.4081094389455302
1102	24	1.3207281868688554
1102	25	1.3072754677683136
1103	1	0.32310094574394554
1103	2	0.5437558747727209
1103	3	0.4603502367186678
1103	4	0.6675924992746332
1103	5	0.26211835140784956
1103	6	0.6118054111102182
1103	7	0.4016649159388024
1103	8	0.5015175996932655
1103	9	0.7995465969551034
1103	10	0.7653566264125544
1103	11	0.7488196065343686
1103	12	0.8467749211508391
1103	13	0.8655721367533431
1103	14	1.0481504595148934
1103	15	1.1085261021565387
1103	16	1.1571110214256777
1103	17	0.8690029190480786
1103	18	1.1905495066668266
1103	19	1.1538663161261198
1103	20	1.078032240508982
1103	21	0.8955810842953653
1103	22	1.008450833310036
1103	23	1.3633871392349306
1103	24	1.1955826465770798
1103	25	1.1167593989601485
1104	1	0.44098674022654905
1104	2	0.39350160091215614
1104	3	0.39157927268370685
1104	4	0.6120654445822353
1104	5	0.31604689304948863
1104	6	0.496396841889684
1104	7	0.6840587578474242
1104	8	0.5883398553812853
1104	9	0.6915831758965538
1104	10	0.841490651110611
1104	11	0.7273275327716746
1104	12	0.7013723317852865
1104	13	0.7368259781819759
1104	14	0.8736761751157971
1104	15	0.9488052598084593
1104	16	0.8402623617610132
1104	17	0.878959506040367
1104	18	1.116083174102549
1104	19	0.8257756168339938
1104	20	0.9533787390479134
1104	21	1.0356699180080895
1104	22	0.9005532075750153
1104	23	1.1691511980820124
1104	24	1.1242759307732157
1104	25	0.893143026050808
1105	1	0.49213824784382254
1105	2	0.7499993107583656
1105	3	0.6391820169741997
1105	4	0.8012533439418732
1105	5	0.524727065132403
1105	6	0.43107121305632035
1105	7	0.6738692272219282
1105	8	0.7347950874550975
1105	9	0.6644237263687813
1105	10	0.7709622798047988
1105	11	0.6796365150034092
1105	12	0.8002034329662067
1105	13	0.651816936169709
1105	14	0.8449759320566272
1105	15	1.0199104920266109
1105	16	0.9999983545345259
1105	17	0.9071774142672737
1105	18	0.9585709088983592
1105	19	1.0024928912939401
1105	20	0.9971155693507541
1105	21	1.009299782746119
1105	22	0.7150982592022856
1105	23	1.0082110572903602
1105	24	1.1449379560598913
1105	25	1.1268907283883607
1106	1	0.508791064032998
1106	2	0.5876026920525419
1106	3	0.6415531794893106
1106	4	0.632234486757369
1106	5	0.6612296966618274
1106	6	0.6492206164007968
1106	7	0.48750585750833153
1106	8	0.7641576093441529
1106	9	0.8300921104456194
1106	10	0.8834490599393376
1106	11	0.7832401094950185
1106	12	0.9522909932390817
1106	13	0.9549989538793184
1106	14	1.0321206783427925
1106	15	1.1482864884472477
1106	16	1.0547410565527358
1106	17	0.9870593305946969
1106	18	1.2297297889853454
1106	19	0.9219932050521535
1106	20	1.0165143905859324
1106	21	0.8392055596549213
1106	22	1.0034081313163128
1106	23	1.2239815131644676
1106	24	1.0071824371242784
1106	25	1.150574094764849
1107	1	0.41018771329039083
1107	2	0.6098678947772456
1107	3	0.7198341330486606
1107	4	0.8172530682271244
1107	5	0.6682259673434814
1107	6	0.6666521406457093
1107	7	0.8174289701650039
1107	8	0.7430684009234483
1107	9	0.9361231649440285
1107	10	0.7513751855726483
1107	11	0.7903432887497672
1107	12	1.0772654871328058
1107	13	0.9984678711613881
1107	14	1.1430923198940874
1107	15	1.2699967434214798
1107	16	1.1327604368520383
1107	17	1.154423345060458
1107	18	1.5028236688222805
1107	19	1.3774899337583548
1107	20	1.3369774512318529
1107	21	1.1911040822544194
1107	22	1.151199193976673
1107	23	1.3348560144386923
1107	24	1.2045204679226273
1107	25	1.18995118138141
1108	1	0.5583034823042183
1108	2	0.5576164187110564
1108	3	0.45544817752163524
1108	4	0.6274685208639639
1108	5	0.5432655027357498
1108	6	0.6547341555851602
1108	7	0.7182817145547347
1108	8	0.7827315499960434
1108	9	0.8161759523182326
1108	10	0.8186519877530287
1108	11	0.7771611096621811
1108	12	0.7621402549650327
1108	13	0.8240935627374597
1108	14	1.0123131136802925
1108	15	0.9216790271546742
1108	16	0.9362155720419594
1108	17	1.02182175669968
1108	18	1.249381274117816
1108	19	1.1002895838759983
1108	20	0.9769364939732483
1108	21	0.918722122969541
1108	22	0.8588177227251208
1108	23	1.1620762610644562
1108	24	1.0343580279061442
1108	25	1.110666882258279
1109	1	0.0239586411291981
1109	2	0.07273065916484711
1109	3	0.21812164830616965
1109	4	0.29392729533175554
1109	5	0.24672699665612186
1109	6	0.12467121481087418
1109	7	0.3319031098235996
1109	8	0.2959709714723573
1109	9	0.5210710616848431
1109	10	0.5766197611861372
1109	11	0.14238870603128362
1109	12	0.4492435891896136
1109	13	0.3650853494365123
1109	14	0.3404448002642724
1109	15	0.5737874377065211
1109	16	0.4314569444762384
1109	17	0.3669832364816372
1109	18	-1.051645643086772
1109	19	0.16305131904920245
1109	20	0.44831628637786736
1109	21	0.46465074746365076
1109	22	0.5849178363770662
1109	23	0.23244396146097301
1109	24	0.6074052759968112
1109	25	0.6549545516971307
1110	1	0.294706563065521
1110	2	0.24412874661635442
1110	3	0.33397114587848264
1110	4	0.19616444434352331
1110	5	0.2930077839037608
1110	6	0.3243732862839508
1110	7	0.3536760875954741
1110	8	0.2696214847950742
1110	9	0.6044986697918375
1110	10	0.7478778802177573
1110	11	0.6399511955902142
1110	12	0.21832665493976577
1110	13	0.3961223195554311
1110	14	0.6850679470520333
1110	15	0.6459172545759131
1110	16	0.714004305419607
1110	17	0.31394729109111097
1110	18	0.8677203422414834
1110	19	0.5882997287614771
1110	20	0.6540218778394049
1110	21	0.588277571533169
1110	22	0.7494518153631091
1110	23	0.8004690302753019
1110	24	0.9291920779571541
1110	25	0.8939096978614316
1111	1	0.0143048543620381
1111	2	0.33594712314366504
1111	3	0.2974997052511057
1111	4	0.18081952340710217
1111	5	0.2262374115376673
1111	6	0.3553686187460201
1111	7	0.23898865670580705
1111	8	0.1831102408238081
1111	9	0.427854357077436
1111	10	0.4156363060503594
1111	11	0.19263494724788188
1111	12	0.3932094976156577
1111	13	0.3626695972297256
1111	14	0.3371214996364742
1111	15	0.4999406624166056
1111	16	0.3573268504026714
1111	17	0.30734948784616734
1111	18	0.5024292187869221
1111	19	0.24653264303410083
1111	20	0.21527385305366664
1111	21	0.3991375996765356
1111	22	0.1847653853428497
1111	23	0.5549403767668508
1111	24	0.6336827114726233
1111	25	0.09019810141582241
1112	1	-0.14040082161602996
1112	2	0.05088422871553888
1112	3	0.15121038545453658
1112	4	0.14271635071157363
1112	5	0.177846167539073
1112	6	0.18683134249523903
1112	7	-0.06791346692713063
1112	8	0.25768059178049896
1112	9	0.41361879341675234
1112	10	0.29647741824677354
1112	11	0.32027712650101847
1112	12	0.361304229231159
1112	13	0.0537621291472945
1112	14	0.2495051634270169
1112	15	0.17436408141462323
1112	16	0.4021617813984406
1112	17	0.45696060599922106
1112	18	0.37291013843649523
1112	19	0.2999529568386947
1112	20	0.16143329430941122
1112	21	0.1795030855367301
1112	22	0.17951518794411214
1112	23	0.3238498010475652
1112	24	0.11523456767202589
1112	25	0.32559538876458305
1113	1	-0.19020357554452616
1113	2	-0.00506726178342376
1113	3	-0.13266764974802925
1113	4	0.15767892740210243
1113	5	-0.14782528408240125
1113	6	-0.07138097020029477
1113	7	-0.12214764353323915
1113	8	0.19343271619887048
1113	9	0.030055705627660245
1113	10	-0.2839427176018958
1113	11	-0.17670638820889906
1113	12	-0.020862564466384015
1113	13	-0.09724087425809813
1113	14	0.04767071045031912
1113	15	-0.44665979182895654
1113	16	-0.27149767980201744
1113	17	-0.34806348409844645
1113	18	-0.2843184237558205
1113	19	-0.3901313664931152
1113	20	-0.48571516326265396
1113	21	-0.27751168835796847
1113	22	-0.173887071528668
1113	23	-0.5856668854125573
1113	24	-0.15619678883668087
1113	25	-0.14007881141138512
1114	1	-1.3770347593289216
1114	2	-1.1330461037430517
1114	3	-1.0637514399213046
1114	4	-1.3072938547705704
1114	5	-1.3158549194212839
1114	6	-1.7031256626442257
1114	7	-1.8411210511423697
1114	8	-1.3343880417937264
1114	9	-1.4358871882670527
1114	10	-1.575332073769765
1114	11	-2.275166614218996
1114	12	-1.690638849839317
1114	13	-1.6880940255824086
1114	14	-2.0044290087412335
1114	15	-2.0609002970738715
1114	16	-2.669041997007696
1114	17	-4.6476910221577405
1114	18	-3.614925209351501
1114	19	-2.6069733991308794
1114	20	-1.7676828638527269
1114	21	-2.7296251591179868
1114	22	-2.858922754131598
1114	23	-2.7017605444627364
1114	24	-2.310141699155537
1114	25	-2.134458094082788
1115	1	-0.8936849249354708
1115	2	-0.6316537157953519
1115	3	-0.7714086974711627
1115	4	-0.8709069215263341
1115	5	-0.5969383819491156
1115	6	-0.8324925926253428
1115	7	-1.7980640586671832
1115	8	-1.184521277691477
1115	9	-0.9041181285954139
1115	10	-0.8619842592860394
1115	11	-1.6104989793533235
1115	12	-1.1726597588649952
1115	13	-0.9929658915551229
1115	14	-1.0582141372328309
1115	15	-2.3160847517728342
1115	16	-1.0806520024043849
1115	17	-1.14407069509339
1115	18	-1.1990424910408208
1115	19	-1.2581969126797787
1115	20	-0.9935252289610048
1115	21	-1.63798850890975
1115	22	-1.6947435206506327
1115	23	-1.0515764674608405
1115	24	-1.0781441741961544
1115	25	-1.3936176032518333
1116	1	-1.285098729381616
1116	2	-2.0240744529487493
1116	3	-1.6661421177484392
1116	4	-1.3751285599294008
1116	5	-1.690055276845527
1116	6	-1.599036170795216
1116	7	-1.3126147420834045
1116	8	-2.0793812039065784
1116	9	-2.0789097802492122
1116	10	-2.944665948750675
1116	11	-1.6209791949840024
1116	12	-1.4883085547398807
1116	13	-2.1808158430100377
1116	14	-1.9758272873034244
1116	15	-1.5746514088085348
1116	16	-1.4886485416202007
1116	17	-1.8161510505739358
1116	18	-1.6956553599113349
1116	19	-1.9517939618293312
1116	20	-2.32670038251284
1116	21	-2.763824181220045
1116	22	-1.8944462714784933
1116	23	-1.9351163286521142
1116	24	-2.4126401952263805
1116	25	-2.113461167804978
1201	1	0.3624488720127168
1201	2	0.4064623220218273
1201	3	0.6093292547688449
1201	4	0.668878704497402
1201	5	0.6970988030274654
1201	6	0.723667067134997
1201	7	0.6808376339101869
1201	8	0.1732188872557927
1201	9	0.5424176913994074
1201	10	0.7763329337367466
1201	11	0.37368447419419226
1201	12	-0.1803208985529139
1201	13	0.28991580044751686
1201	14	0.8055262001331869
1201	15	0.42173530052751573
1201	16	0.9424319942621864
1201	17	0.9911314658882446
1201	18	1.2844254247038833
1201	19	0.7701401084989854
1201	20	0.8023194792202659
1201	21	0.611380725838373
1201	22	0.9773332631649936
1201	23	1.1490097562812167
1201	24	0.9803661278240128
1201	25	0.8922699222517778
1202	1	0.6309724361248357
1202	2	0.7388152631982976
1202	3	0.46505095791372497
1202	4	0.8951790039486198
1202	5	0.848118607934353
1202	6	0.9383652382977132
1202	7	0.9447473358726839
1202	8	0.922152172608385
1202	9	1.2173409007967777
1202	10	1.3014354283067817
1202	11	0.9152683526658336
1202	12	1.0285707463724805
1202	13	1.119591030819322
1202	14	1.220267425441314
1202	15	0.927590021703729
1202	16	1.051300505616915
1202	17	1.239290419222698
1202	18	1.5664275067252476
1202	19	1.3603481821151533
1202	20	1.2706541191719438
1202	21	1.2821636686855982
1202	22	1.4422068639052625
1202	23	1.4526256618962492
1202	24	1.5658671624087006
1202	25	1.370850806211216
1203	1	0.3317095520103628
1203	2	0.5188177929225581
1203	3	0.5085927879848988
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2211	19	0.3694936127524002
2211	20	0.252484136568043
2211	21	0.2858313150468845
2211	22	0.2319380128548545
2211	23	0.7597111883672483
2211	24	0.635628558964001
2211	25	0.6192952771450777
2212	1	-0.09811789984245323
2212	2	-0.14723832998075181
2212	3	-0.0884935612717257
2212	4	-0.2728242278008679
2212	5	-0.18627871363084836
2212	6	-0.24001050154645043
2212	7	-0.3518565728303926
2212	8	-0.23716175390603667
2212	9	-0.18967591286960328
2212	10	-0.19042521473791396
2212	11	-0.33199414741067557
2212	12	-0.0996847209764482
2212	13	-0.26445710266172284
2212	14	-0.15714766462534158
2212	15	-0.26241695593721204
2212	16	-0.1199384541025097
2212	17	-0.30508860133686255
2212	18	-3.011026756062506
2212	19	-1.2221324304482764
2212	20	-0.5771497601515918
2212	21	-0.652323321686481
2212	22	-0.392326031999346
2212	23	-0.4215616571594438
2212	24	-0.8010768756867677
2212	25	-0.43365099634603155
2213	1	-0.1703389523889136
2213	2	-0.1710322902809267
2213	3	-0.4235511996654182
2213	4	-0.6463834925762981
2213	5	-0.29330747996766604
2213	6	-0.3061889192616576
2213	7	-0.20766699790586785
2213	8	-0.5706131662570755
2213	9	-0.530561631288279
2213	10	-0.6712080941987821
2213	11	-0.5312152332005482
2213	12	-0.539385027085423
2213	13	-0.39460819249172374
2213	14	-0.4959659420353617
2213	15	-0.7155811451707166
2213	16	-1.0774261742540432
2213	17	-0.7447721359925268
2213	18	-0.5991749090912322
2213	19	-0.6484154541136959
2213	20	-1.3300470137362694
2213	21	-0.8834050774300266
2213	22	-0.8769962947451262
2213	23	-1.2216972064410072
2213	24	-1.0093589188211496
2213	25	-1.1951442786302522
2214	1	-1.7013867481386455
2214	2	-3.642247063322017
2214	3	-1.911477723849874
2214	4	-1.694685027285015
2214	5	-1.6856519354208324
2214	6	-1.708853634263427
2214	7	-1.807774483418065
2214	8	-1.397957396179283
2214	9	-1.6275570687379997
2214	10	-1.7523389951605424
2214	11	-1.4949154200965395
2214	12	-1.9137349658880396
2214	13	-1.8422362737580684
2214	14	-3.1293264941947143
2214	15	-3.1466796993466346
2214	16	-2.464418069595766
2214	17	-2.868838796119725
2214	18	-2.331091250320135
2214	19	-4.442236910899762
2214	20	-2.848639358055223
2214	21	-2.672888211964178
2214	22	-2.9035274219575555
2214	23	-2.8197414093925666
2214	24	-2.953498591417201
2214	25	-2.8854819552493822
2215	1	-0.860090036763971
2215	2	-2.4971852314838685
2215	3	-1.3382177352925986
2215	4	-0.9458423282876325
2215	5	-0.8954038522518175
2215	6	-1.3848297000348104
2215	7	-1.8652456789168177
2215	8	-1.8877671370241202
2215	9	-1.5311738719290773
2215	10	-2.9830969292514737
2215	11	-1.9620916106431636
2215	12	-2.297649956449078
2215	13	-1.7089948866482985
2215	14	-3.1656899399162697
2215	15	-2.848466677821598
2215	16	-2.469004146508418
2215	17	-2.664748325080069
2215	18	-1.6508454770409742
2215	19	-2.17001486239257
2215	20	-2.455992035171377
2215	21	-2.1567962185566927
2215	22	-1.8125816097516676
2215	23	-2.3132352404388214
2215	24	-2.0577593177536713
2215	25	-2.000475899043977
2216	1	-1.9470576997543603
2216	2	-2.0655014610246916
2216	3	-2.566979073843484
2216	4	-3.2240974473755877
2216	5	-2.3670266794299515
2216	6	-2.35418966745949
2216	7	-2.239340220805623
2216	8	-2.7877063206472386
2216	9	-3.1180799131892805
2216	10	-2.6439054844464245
2216	11	-3.2057694956780054
2216	12	-3.526575111183682
2216	13	-4.170414497562593
2216	14	-3.5998142359490117
2216	15	-3.4547413440545114
2216	16	-2.6241593045339897
2216	17	-2.5991960436788872
2216	18	-2.6069938675362962
2216	19	-3.0353146411278544
2216	20	-2.9147220711035615
2216	21	-3.2772050793466185
2216	22	-3.72952289986177
2216	23	-3.7611659156262895
2216	24	-3.6766204359184016
2216	25	-3.7386756122649842
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	108472.0
3	2.0
4	849.0
5	5332.0
6	6644.0
7	6009.0
8	5426.0
9	5051.0
10	4734.0
11	4670.0
12	4331.0
13	4252.0
14	4375.0
15	4462.0
16	4599.0
17	4930.0
18	5638.0
19	6584.0
20	8547.0
21	11045.0
22	13686.0
23	16840.0
24	19406.0
25	22252.0
26	24913.0
27	27659.0
28	31775.0
29	38236.0
30	46654.0
31	58727.0
32	73789.0
33	95743.0
34	125101.0
35	166698.0
36	231004.0
37	370459.0
38	1022626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.05722292014877	18.775278322952214	12.74325666123988	25.42424209565914
2	14.289850350556312	19.937316007155502	42.31613454629961	23.45669909598858
3	19.02002095278829	24.345611379047057	29.542119041792088	27.092248626372566
4	12.170267399165594	16.826167381563383	38.44893661043224	32.55462860883878
5	12.877802320993869	38.22525267888646	36.13379492766099	12.763150072458677
6	31.96236570552145	37.03102343806987	18.26694367244671	12.739667183961966
7	28.00528743173617	31.430269581829762	22.242218405665657	18.322224580768413
8	24.552991443094843	37.952170875645045	20.63433800740286	16.860499673857245
9	26.359536467931953	15.200648404558128	20.258126766950664	38.18168836055925
10	14.769007627636412	29.147968736069423	35.02853947146497	21.054484164829198
11	34.0036371860592	22.162867510557643	25.3235219879167	18.509973315466453
12	23.61942945448735	24.52089815723232	32.06530003543042	19.79437235284991
13	29.095791056907743	22.140245613344433	25.66848180786983	23.09548152187799
14	20.368736379658127	22.891360717988384	27.55707323700765	29.18282966534584
15	22.739827864783766	31.947456223169006	23.10045646942936	22.212259442617864
16	21.504787444267418	28.165602961700447	28.91644898538911	21.41316060864303
17	21.37815617749896	29.170125874644093	28.293715994104	21.158001953752947
18	21.303367835117655	27.516663415425562	30.614484568837142	20.56548418061964
19	23.43272054694167	27.069027339750036	27.55550992476194	21.942742188546354
20	23.50374733780384	27.76044364403803	28.177440632736193	20.558368385421932
21	22.400500844132335	27.136017625267	27.31071094701072	23.152770583589945
22	22.827080367921994	28.901915256906257	26.982683026988973	21.288321348182777
23	21.685140820798196	28.534221667463683	27.997809269845387	21.782828241892734
24	23.046759667543416	27.258429611561493	28.57208468495937	21.122726035935724
25	22.452599775523403	27.626985977160203	28.11901273526429	21.801401512052106
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	174.0
1	174.0
2	375.0
3	576.0
4	576.0
5	576.0
6	1084.5
7	1593.0
8	1593.0
9	1593.0
10	2526.5
11	3460.0
12	3460.0
13	3460.0
14	6415.0
15	9370.0
16	9370.0
17	9370.0
18	18106.0
19	26842.0
20	26842.0
21	26842.0
22	45230.0
23	63618.0
24	63618.0
25	63618.0
26	94722.5
27	125827.0
28	125827.0
29	125827.0
30	166730.5
31	207634.0
32	207634.0
33	207634.0
34	234746.0
35	261858.0
36	261858.0
37	261858.0
38	281934.5
39	302011.0
40	302011.0
41	302011.0
42	315215.0
43	328419.0
44	328419.0
45	328419.0
46	340967.5
47	353516.0
48	353516.0
49	353516.0
50	333082.5
51	312649.0
52	312649.0
53	312649.0
54	273011.0
55	233373.0
56	233373.0
57	233373.0
58	198411.0
59	163449.0
60	163449.0
61	163449.0
62	132907.5
63	102366.0
64	102366.0
65	102366.0
66	78437.5
67	54509.0
68	54509.0
69	54509.0
70	39668.5
71	24828.0
72	24828.0
73	24828.0
74	17392.0
75	9956.0
76	9956.0
77	9956.0
78	6827.0
79	3698.0
80	3698.0
81	3698.0
82	2481.5
83	1265.0
84	1265.0
85	1265.0
86	822.0
87	379.0
88	379.0
89	379.0
90	247.0
91	115.0
92	115.0
93	115.0
94	68.0
95	21.0
96	21.0
97	21.0
98	17.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3576279557942829
2	0.0411341606470334
3	0.036040624807063035
4	0.03611779959251713
5	0.041597209359757976
6	0.018483361116256095
7	0.018136074581712663
8	0.024310057418040376
9	0.02103012903624128
10	0.021107303821695376
11	0.019525220719886396
12	0.020296968574427362
13	0.020721429894424895
14	0.028014447119837006
15	0.029557942828918934
16	0.022226338210779774
17	0.023499722170772366
18	0.10669414089028832
19	0.026123664876211643
20	0.023692659134407608
21	0.026779650552571466
22	0.020682842501697843
23	0.023268197814410075
24	0.01948663332715935
25	0.023306785207137123
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2591520.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.06811749002942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.6612443093216	52.63068448430198
2	13.258293190923117	17.519009445103315
3	3.7782488812052617	7.4886537297012445
4	1.4727818782502768	3.8921570467770206
5	0.6592807950593477	2.17787205134505
6	0.3393764669034074	1.3453178573235243
7	0.1970379281921713	0.911254749285465
8	0.13292228824813299	0.7025540285616966
9	0.08504709820504991	0.5057011508757577
>10	0.3684663488279222	4.279355164851329
>50	0.024036099956787845	1.1123850550677055
>100	0.018175621067336455	2.5586451597978157
>500	0.0025152992541368794	1.1368718664625468
>1k	0.002281317928087236	2.5805990416792315
>5k	2.9247665744708154E-4	1.1589391688662771
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	7156	0.27613138235475704	No Hit
CTGTAGGACGTGGAATATGGCAAGA	6523	0.25170556275853556	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5710	0.22033401247144535	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5337	0.20594091498425637	No Hit
GTACATGGGAAGCAGTGGTATCAAC	5262	0.20304686052972773	No Hit
CCCATGTACTCTGCGTTGATACCAC	4449	0.17167531024263752	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3411	0.1316215965919615	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3206	0.1237111810829166	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2972	0.1146817311847873	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.858739272704822E-5
2	0.0	0.0	0.0	0.0	3.858739272704822E-5
3	0.0	0.0	0.0	0.0	3.858739272704822E-5
4	0.0	0.0	0.0	0.0	3.858739272704822E-5
5	0.0	0.0	0.0	3.858739272704822E-5	3.858739272704822E-5
6	0.0	0.0	0.0	3.858739272704822E-5	3.858739272704822E-5
7	0.0	0.0	0.0	3.858739272704822E-5	3.858739272704822E-5
8	0.0	0.0	0.0	3.858739272704822E-5	3.858739272704822E-5
9	0.0	0.0	0.0	7.717478545409643E-5	3.858739272704822E-5
10	0.0	0.0	0.0	7.717478545409643E-5	3.858739272704822E-5
11	0.0	0.0	0.0	7.717478545409643E-5	3.858739272704822E-5
12	0.0	0.0	0.0	7.717478545409643E-5	3.858739272704822E-5
13	0.0	0.0	0.0	7.717478545409643E-5	3.858739272704822E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCG	40	0.0053000064	14.242025	8
GGACGTA	85	3.970954E-6	12.287237	6
GTACCGT	70	1.09716035E-4	12.207451	6
GACCGTC	70	1.09716035E-4	12.207451	7
TAGGACC	1055	0.0	11.882814	4
GCGTTTC	80	2.8601637E-5	11.878212	13
GGCGTGC	145	9.822543E-11	11.786504	8
TCCAACG	225	0.0	11.402863	18
AGGACGT	3460	0.0	11.390769	5
GGACGTG	3375	0.0	11.337355	6
GGCGAGG	1560	0.0	11.260156	19
GACGTGG	1720	0.0	11.040329	7
GCGCCAC	150	2.1263986E-9	10.769578	13
GTCCTAA	1725	0.0	10.716339	1
TTAGGAC	2115	0.0	10.642513	3
ATAGGAC	395	0.0	10.579418	3
GACGTGA	1885	0.0	10.577631	7
TGTAGGA	3425	0.0	10.565027	2
TAGGACG	3770	0.0	10.530107	4
GTCCTAC	3240	0.0	10.528628	1
>>END_MODULE