FastQCFastQC Report
Thu 26 May 2016
SRR1512984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512984_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2591520
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT68940.26602148546027043No Hit
CTGTAGGACGTGGAATATGGCAAGA65840.2540593937148855No Hit
CTTTAGGACGTGAAATATGGCGAGG55370.213658393529666No Hit
GTCCTAAAGTGTGTATTTCTCATTT54060.20860344508242265No Hit
GTACATGGGAAGCAGTGGTATCAAC52110.20107890350064828No Hit
CCCATGTACTCTGCGTTGATACCAC50700.19563808112613448No Hit
GTATCAACGCAGAGTACTTTTTTTT36750.1418086682719022No Hit
GTCCTACAGTGTGCATTTCTCATTT33390.128843304315614No Hit
CTGAAGGACCTGGAATATGGCGAGA28890.1114789775884423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2300.013.61692418
TAGGACC9950.013.577364
GGCGAGG13750.012.14794719
TATGTCG1105.0190647E-711.21917616
ACCGAGT855.3052714E-511.180718
TATTCCG600.005814677611.0993335
TGTCGAG951.3760953E-510.98909718
AGGACCT20900.010.8793195
GTTCTAA2450.010.8635691
TGTAGGA34300.010.7527182
AGGACGT34400.010.6752725
GTAGGAC33700.010.6342993
GGACGTG34250.010.6080446
TGGCGAG33550.010.57966718
GACGTGG17100.010.4492137
GTCGAGG1002.4264751E-510.43964219
GAACAGT5750.010.4208996
GGACCTG20200.010.405756
CTGTAGG35650.010.3721961
GTAGAAC7650.010.3136893
Produced by FastQC (version 0.11.5)