FastQCFastQC Report
Thu 26 May 2016
SRR1512983_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512983_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3014005
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT76780.2547441029460801No Hit
CTGTAGGACGTGGAATATGGCAAGA69020.22899762940008392No Hit
GTCCTAAAGTGTGTATTTCTCATTT67320.22335729370057447No Hit
CTTTAGGACGTGAAATATGGCGAGG58710.19479065230482365No Hit
GTCCTACAGTGTGCATTTCTCATTT44530.14774361688185655No Hit
GTATCAACGCAGAGTACTTTTTTTT38270.12697391012954526No Hit
CTGAAGGACCTGGAATATGGCGAGA33800.1121431450843645No Hit
GTACATGGGAAGCAGTGGTATCAAC31180.10345039241806168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11950.013.4325884
TCGGAAC752.0787216E-411.3978283
AGGACGT38450.011.3383545
TCCAACG3200.011.28434718
GGACGTG37350.011.1846586
TGTAGGA39850.011.1308782
GGCGAGG16450.011.08143419
GGACCTG23400.010.9549046
AGGACCT24200.010.9502125
GACGTGA20350.010.9172217
CTGTAGG39450.010.9144821
GACGTGG18750.010.886747
CCAACGA2100.010.85057119
GTACTAG1855.456968E-1210.8133261
GTAGGAC38650.010.7637973
TTAGGAC23750.010.6379743
GTCCTAC36800.010.4579181
TGGCGAG38750.010.44673818
GTCCTAA19800.010.4401381
TAGGACG42400.010.2822394
Produced by FastQC (version 0.11.5)