##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512983_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3014005
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.041634303858157	31.0	30.0	34.0	23.0	34.0
2	29.154863379456902	31.0	30.0	34.0	23.0	34.0
3	29.31166073048983	31.0	30.0	34.0	23.0	34.0
4	32.784761803646646	35.0	33.0	37.0	26.0	37.0
5	32.888258645888115	35.0	35.0	37.0	26.0	37.0
6	32.996848047697334	37.0	35.0	37.0	26.0	37.0
7	32.909796101864465	37.0	35.0	37.0	26.0	37.0
8	32.91666503539311	37.0	35.0	37.0	26.0	37.0
9	34.267984625108454	39.0	35.0	39.0	25.0	39.0
10	34.124708817669514	39.0	35.0	39.0	25.0	39.0
11	34.203717644794885	39.0	35.0	39.0	25.0	39.0
12	34.0857855245761	39.0	35.0	39.0	25.0	39.0
13	34.099157433381826	39.0	35.0	39.0	25.0	39.0
14	35.114145796042145	40.0	35.0	41.0	25.0	41.0
15	35.06102312371745	40.0	35.0	41.0	24.0	41.0
16	34.95225555365701	39.0	35.0	41.0	23.0	41.0
17	34.91268030411363	39.0	35.0	41.0	22.0	41.0
18	34.609826128357454	39.0	34.0	41.0	18.0	41.0
19	34.84860409986049	39.0	34.0	41.0	21.0	41.0
20	34.92371810929312	40.0	35.0	41.0	22.0	41.0
21	34.859632283290836	40.0	35.0	41.0	22.0	41.0
22	34.78598476114008	40.0	35.0	41.0	19.0	41.0
23	34.526619564333835	39.0	34.0	41.0	18.0	41.0
24	34.53359699137858	39.0	34.0	41.0	18.0	41.0
25	34.459320405905096	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.41762801726371634
1101	2	0.7493758511665582
1101	3	0.640774758456562
1101	4	0.694991090977048
1101	5	0.6008224944476623
1101	6	0.7344550068752227
1101	7	0.8006930566310828
1101	8	0.6586287949967016
1101	9	0.6033598312628143
1101	10	0.6330259263109212
1101	11	0.7427676652983166
1101	12	0.9597572027331438
1101	13	0.7345854190419914
1101	14	0.7527177786365371
1101	15	0.5084059665587617
1101	16	1.063258678849003
1101	17	0.9725096131605255
1101	18	1.2469097668492424
1101	19	0.7188208751467755
1101	20	0.8333424343650364
1101	21	0.7861097891984272
1101	22	0.8015254099344418
1101	23	1.020490825177788
1101	24	0.6525214100017251
1101	25	0.9275390998616473
1102	1	0.4581253511571184
1102	2	0.5573465633814436
1102	3	0.5472014096817297
1102	4	0.6788388891213089
1102	5	0.5395746852991579
1102	6	0.5024125236863952
1102	7	0.6868779973402326
1102	8	0.6828610244066979
1102	9	0.7724559727706719
1102	10	0.8869534858714232
1102	11	0.8844769936294838
1102	12	0.9661842660116946
1102	13	0.7075845302185968
1102	14	0.7917338533238834
1102	15	0.9624904942207522
1102	16	0.8728039682425788
1102	17	0.8001926684830565
1102	18	1.1005675526343168
1102	19	1.106432853684126
1102	20	1.0296365716452271
1102	21	0.7081188936391811
1102	22	1.0882765604723303
1102	23	1.3063809295703024
1102	24	1.1278336676931318
1102	25	0.9993210780523896
1103	1	0.29579334414235703
1103	2	0.48383416669500434
1103	3	0.43252098549303497
1103	4	0.6098694298119725
1103	5	0.1810915227356915
1103	6	0.580671479910734
1103	7	0.43643600087620626
1103	8	0.47869698413672523
1103	9	0.8195134303359097
1103	10	0.7974696346606294
1103	11	0.7805941400450109
1103	12	0.8806568921393989
1103	13	0.8206291583650298
1103	14	1.0523254974980603
1103	15	0.98279656137111
1103	16	1.0416038709553916
1103	17	0.9127159204408812
1103	18	1.2393643177788718
1103	19	1.2156684777244493
1103	20	1.0622355390692562
1103	21	0.867095649487645
1103	22	0.9987739929520671
1103	23	1.3276922694530242
1103	24	1.103461364746238
1103	25	1.109345514592718
1104	1	0.5072864267872745
1104	2	0.5130750005770395
1104	3	0.4845448259761156
1104	4	0.6843609797753629
1104	5	0.5511990686561887
1104	6	0.6708511596153386
1104	7	0.8107594354107874
1104	8	0.8084299913833064
1104	9	0.8776698413158854
1104	10	0.9983050475347852
1104	11	0.9431911775984716
1104	12	0.850410224476768
1104	13	0.8591843063121445
1104	14	1.0120470511317663
1104	15	1.089775703253892
1104	16	0.9726047064604799
1104	17	1.0290537337783476
1104	18	1.2632658499070857
1104	19	1.0755990920696803
1104	20	1.0986184659929918
1104	21	1.1920596379270592
1104	22	1.1079104163631754
1104	23	1.3864147213008167
1104	24	1.1858510199758925
1104	25	0.947444177969011
1105	1	0.5525840281419967
1105	2	0.7049441117537967
1105	3	0.6914549605062525
1105	4	0.890437186414168
1105	5	0.5896522316511437
1105	6	0.4222568509251161
1105	7	0.7643743557001983
1105	8	0.7721343733178685
1105	9	0.6918092191869363
1105	10	0.7817196588224888
1105	11	0.7717034993057297
1105	12	0.8656033137909418
1105	13	0.8441623486305829
1105	14	0.9050749416828694
1105	15	1.0303026600464804
1105	16	1.0246972673109838
1105	17	0.9558732075933705
1105	18	1.0983459050551119
1105	19	1.0079785752016193
1105	20	0.9912821333002384
1105	21	1.0266428830146666
1105	22	0.7980440460490286
1105	23	1.059608294837119
1105	24	1.1000444249073311
1105	25	1.076195257017126
1106	1	0.5688958681118308
1106	2	0.627309056525764
1106	3	0.6117133486404533
1106	4	0.5954427561211801
1106	5	0.6858533082864469
1106	6	0.7343270015792243
1106	7	0.59784327912093
1106	8	0.6811848896476889
1106	9	0.8075845670473569
1106	10	0.9132247973584455
1106	11	0.8301545152553516
1106	12	0.8048338609814465
1106	13	0.7518023183591396
1106	14	0.8822746596458444
1106	15	1.0989234296041275
1106	16	1.0374117073570588
1106	17	1.0557635938073204
1106	18	1.2620013481400818
1106	19	0.7081924617111781
1106	20	0.8256376188787371
1106	21	0.6963558230445628
1106	22	0.8262104692198733
1106	23	1.0441908795028993
1106	24	1.0015318701569242
1106	25	1.0593630023494782
1107	1	0.5141841300131276
1107	2	0.6680521246453281
1107	3	0.8228308457800217
1107	4	0.8356415008947593
1107	5	0.7404882901004299
1107	6	0.6671481659871148
1107	7	0.9052359982694185
1107	8	0.8681726720302549
1107	9	1.0271742936391703
1107	10	0.8804776624837487
1107	11	0.8932579196931556
1107	12	1.1582725602430628
1107	13	1.0590938466962854
1107	14	1.2582180891131074
1107	15	1.3366100217419472
1107	16	1.1962116958834557
1107	17	1.3115092487995952
1107	18	1.5637628827416776
1107	19	1.4656269320290676
1107	20	1.2838019910017024
1107	21	1.3810666793755644
1107	22	1.3184626880696584
1107	23	1.3736768372637442
1107	24	1.4180942474309717
1107	25	1.3774200644057402
1108	1	0.3443259017748055
1108	2	0.3662670915321371
1108	3	0.3018726508086722
1108	4	0.4919298255295388
1108	5	0.43946192787785776
1108	6	0.5673569595695938
1108	7	0.614802834664836
1108	8	0.667717752318211
1108	9	0.7033049907855968
1108	10	0.7021968969826986
1108	11	0.4854103602200439
1108	12	0.5591503170094967
1108	13	0.4969399284227478
1108	14	0.7087656331370482
1108	15	0.6803637669282239
1108	16	0.6748499488050967
1108	17	0.7376118404773351
1108	18	0.8548577622081055
1108	19	0.7851686113785519
1108	20	0.7574199708654632
1108	21	0.7739595664294114
1108	22	0.6253082080402734
1108	23	0.7988072271944233
1108	24	0.8707923901013999
1108	25	1.0668172498286523
1109	1	0.04275734526757802
1109	2	-0.023736310538609473
1109	3	0.21058673359286573
1109	4	0.3256147002701084
1109	5	0.19446466317107536
1109	6	0.1140356370867579
1109	7	0.21933358355783383
1109	8	0.30076138719614676
1109	9	0.3908401559950079
1109	10	0.5200723259384858
1109	11	0.14115148300256664
1109	12	0.4182400797872319
1109	13	0.42902053465368795
1109	14	0.20654795586410302
1109	15	0.4882246047018697
1109	16	0.3976890361717267
1109	17	0.22337441348416576
1109	18	-1.235981777115036
1109	19	0.15784235031451743
1109	20	0.290829155677244
1109	21	0.3877404146306418
1109	22	0.5967545007483466
1109	23	0.033318994622419495
1109	24	0.4831912466797661
1109	25	0.6105172559031473
1110	1	0.16500429173042974
1110	2	0.1304565416916006
1110	3	0.22339852092422063
1110	4	-0.050731585435364934
1110	5	0.10634404473103842
1110	6	0.14721772568830005
1110	7	0.22584813483038602
1110	8	-0.014088152894437656
1110	9	0.27531491537161656
1110	10	0.3812228825492312
1110	11	0.25038662629746256
1110	12	-0.21867529323639445
1110	13	0.031036625964972586
1110	14	0.1255011622313944
1110	15	0.29536203866204147
1110	16	0.4420336026256848
1110	17	-0.08503778749062718
1110	18	0.4299897611613446
1110	19	0.2515034556036966
1110	20	0.2791893026072003
1110	21	0.3034950756521937
1110	22	0.2814551330559141
1110	23	0.2832019282043774
1110	24	0.4017029904604925
1110	25	0.431844909084532
1111	1	-0.12005544782574162
1111	2	0.21452291767622356
1111	3	0.1719661117673823
1111	4	0.04520603078988472
1111	5	-0.029412585404188007
1111	6	0.1272532638016699
1111	7	0.1239118775874104
1111	8	-0.08789315790597385
1111	9	0.1854998546712352
1111	10	0.12150516653291987
1111	11	0.03389349016842402
1111	12	0.13028936471524588
1111	13	0.125974062434409
1111	14	0.03429605199003305
1111	15	0.21238594193001603
1111	16	0.23288647834635157
1111	17	0.25944822080650454
1111	18	0.3776421751187655
1111	19	0.1166086872068206
1111	20	-0.0023409564645291425
1111	21	0.2586404172644592
1111	22	-0.041730786923324104
1111	23	0.2586980116904911
1111	24	0.3855035618499585
1111	25	-0.03646807733309032
1112	1	0.1192841725710636
1112	2	0.360806073294178
1112	3	0.4565617005977849
1112	4	0.4319017418888862
1112	5	0.3891892784600799
1112	6	0.45071174665377356
1112	7	0.09120606533088704
1112	8	0.36939889546622595
1112	9	0.6095055786428105
1112	10	0.5380325860391224
1112	11	0.5613130848289458
1112	12	0.641411137363221
1112	13	0.25235268008536593
1112	14	0.49999401660652865
1112	15	0.5454551103310266
1112	16	0.7310589505824936
1112	17	0.7587243213234487
1112	18	0.6317542894934647
1112	19	0.5216238582258654
1112	20	0.3703911634880228
1112	21	0.43213410883958403
1112	22	0.48165928636618816
1112	23	0.7629246506076726
1112	24	0.3805328119355593
1112	25	0.6137856899471998
1113	1	-0.36388369869052184
1113	2	-0.19897713841580256
1113	3	-0.48167600871917315
1113	4	-0.1187213757294927
1113	5	-0.3319064567695733
1113	6	-0.1741189440711679
1113	7	-0.4337975232369047
1113	8	-0.1633200491974094
1113	9	-0.3263691072766832
1113	10	-0.5547778638702923
1113	11	-0.4512684985118227
1113	12	-0.2555127811200322
1113	13	-0.3976631613400201
1113	14	-0.3884937285961101
1113	15	-0.6032539751467212
1113	16	-0.6205070228116654
1113	17	-0.6269127432151649
1113	18	-0.6098085673427249
1113	19	-0.6104055641505042
1113	20	-0.7953304490009643
1113	21	-0.7211263151416176
1113	22	-0.47409865148110697
1113	23	-0.9222159630568143
1113	24	-0.4321492513034073
1113	25	-0.4158366116761272
1114	1	-1.2761660768572547
1114	2	-1.0456179351777912
1114	3	-0.9761542471832421
1114	4	-1.1646278970689288
1114	5	-1.1925131176873727
1114	6	-1.526661393762101
1114	7	-1.7979844080497962
1114	8	-1.2501113782475812
1114	9	-1.3997365712750494
1114	10	-1.5741379527446782
1114	11	-2.180823375597406
1114	12	-1.7805292896928435
1114	13	-1.652188581952565
1114	14	-1.9597185740629186
1114	15	-1.8903446596504097
1114	16	-2.4600425298384963
1114	17	-4.6352251656896115
1114	18	-3.646560405297162
1114	19	-2.5566062617833722
1114	20	-1.602572080125647
1114	21	-2.565974345636512
1114	22	-2.782461441287488
1114	23	-2.5920013904540546
1114	24	-2.175187942218905
1114	25	-2.036518292727912
1115	1	-1.3554732687682787
1115	2	-0.8647955790563344
1115	3	-0.9502992779983366
1115	4	-1.1481078783421204
1115	5	-0.9011826974129846
1115	6	-1.0908917425715856
1115	7	-2.0337391692638
1115	8	-1.4892974673437536
1115	9	-1.2156554399300958
1115	10	-1.1535822616141118
1115	11	-1.7836748846520152
1115	12	-1.4734261424789779
1115	13	-1.2968053404902093
1115	14	-1.3626572235193564
1115	15	-2.5283176607888933
1115	16	-1.4614546337659888
1115	17	-1.4965730384827793
1115	18	-1.4599444867427138
1115	19	-1.490164847469515
1115	20	-1.3674063719684924
1115	21	-2.049676105516525
1115	22	-2.076689471938842
1115	23	-1.524021665260527
1115	24	-1.495191080809498
1115	25	-1.7619218145826565
1116	1	-0.9134402783868296
1116	2	-1.7058031793365487
1116	3	-1.2026286636306622
1116	4	-0.9822380955413124
1116	5	-1.4848341324392926
1116	6	-1.2142352717717273
1116	7	-0.9158207928572608
1116	8	-1.519183459014883
1116	9	-1.5454152690950025
1116	10	-2.5297480271003714
1116	11	-1.2161690324115
1116	12	-1.2885615175710825
1116	13	-1.7428177719115254
1116	14	-1.618890715745387
1116	15	-1.1278706874100877
1116	16	-1.0974987419604503
1116	17	-1.5276443306129295
1116	18	-1.197385046379459
1116	19	-1.486018977538187
1116	20	-1.7589289776656898
1116	21	-2.2148788525421352
1116	22	-1.5014411694392749
1116	23	-1.5675299070799724
1116	24	-2.1454367635186316
1116	25	-1.7656684036980295
1201	1	0.4472278718991731
1201	2	0.46905555808021404
1201	3	0.6069937088852875
1201	4	0.7264745459386006
1201	5	0.8081688058224401
1201	6	0.8204157879149747
1201	7	0.6776441827151771
1201	8	0.40019298621000843
1201	9	0.7191286918208988
1201	10	0.9040406788474371
1201	11	0.5839578865863615
1201	12	0.03070148293311803
1201	13	0.3735008817039329
1201	14	0.8988575152124199
1201	15	0.6384340572740896
1201	16	0.9904639473314134
1201	17	1.1214046462113103
1201	18	1.3454492460905954
1201	19	0.9910886602036939
1201	20	0.9191396802963041
1201	21	0.7992591414422492
1201	22	1.0634887643194375
1201	23	1.283078608663196
1201	24	1.2654065523705782
1201	25	1.072550296860065
1202	1	0.5012038862132115
1202	2	0.6261942011480599
1202	3	0.3401070122365155
1202	4	0.7999290166576856
1202	5	0.7154945952706697
1202	6	0.7756643492573971
1202	7	0.7158454980592452
1202	8	0.7677859549222248
1202	9	1.1097972444588393
1202	10	1.219897586821709
1202	11	1.0002188276376742
1202	12	1.0032074068172534
1202	13	1.031821883686618
1202	14	1.2670873619168006
1202	15	0.9592750070298806
1202	16	1.1181620952109057
1202	17	1.35998339760161
1202	18	1.5805766700472645
1202	19	1.3639287395112092
1202	20	1.3054917766259138
1202	21	1.242874161510919
1202	22	1.3514008974013123
1202	23	1.5740467405844711
1202	24	1.5997251847996168
1202	25	1.44654154401902
1203	1	0.39297525973312375
1203	2	0.573027937500374
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2211	15	0.39306838634693975
2211	16	0.3275674024705637
2211	17	0.3020117600313057
2211	18	0.41121950309015887
2211	19	0.17529277272245025
2211	20	0.11127069993882799
2211	21	0.25767772194543426
2211	22	0.04857919391635335
2211	23	0.6491283599630293
2211	24	0.4373448833661868
2211	25	0.40455624350735775
2212	1	-0.23631857122042987
2212	2	-0.26340502091209217
2212	3	-0.26439939107838484
2212	4	-0.4583362184721267
2212	5	-0.31994757904387683
2212	6	-0.39777305858143563
2212	7	-0.41896642200151035
2212	8	-0.2940582589793479
2212	9	-0.2872434413853213
2212	10	-0.1736965248841713
2212	11	-0.43090933316996427
2212	12	-0.28656584967858123
2212	13	-0.41186203498040896
2212	14	-0.36847173110918163
2212	15	-0.44924174907936987
2212	16	-0.3441659626608953
2212	17	-0.3017748694326201
2212	18	-2.9947219178871514
2212	19	-1.3516630561383849
2212	20	-0.7847134915481249
2212	21	-0.7581853943847179
2212	22	-0.5182375632524909
2212	23	-0.47428372899195637
2212	24	-0.9658739780496575
2212	25	-0.5916040623630039
2213	1	-0.1519959419937429
2213	2	-0.03914582114848386
2213	3	-0.3331467297449535
2213	4	-0.5228439789378356
2213	5	-0.37032736134244715
2213	6	-0.30152016378242763
2213	7	-0.17881466240276467
2213	8	-0.525488118948779
2213	9	-0.39126398879590596
2213	10	-0.42914960143788505
2213	11	-0.49921722514299915
2213	12	-0.5255631306756641
2213	13	-0.2705774620377426
2213	14	-0.30604895769135965
2213	15	-0.5025635897499114
2213	16	-1.1217744875695814
2213	17	-0.6301862023399067
2213	18	-0.41801266233572676
2213	19	-0.4375133168880865
2213	20	-0.9653310480903485
2213	21	-0.762491309914779
2213	22	-0.6287874809820053
2213	23	-1.0604706854440025
2213	24	-0.7429468823824337
2213	25	-1.0210015870005904
2214	1	-1.5985474745856116
2214	2	-3.7199242436330096
2214	3	-1.8774259183263808
2214	4	-1.5624220936808406
2214	5	-1.526483024126641
2214	6	-1.6441649640580316
2214	7	-1.7133657411989276
2214	8	-1.4527332949267056
2214	9	-1.7020152106788444
2214	10	-1.68528244777918
2214	11	-1.4899978713712443
2214	12	-1.9868560123797394
2214	13	-1.810388174221977
2214	14	-2.890000439909734
2214	15	-3.1835895824008844
2214	16	-2.482290388875171
2214	17	-2.8102698179988224
2214	18	-2.2690359915605285
2214	19	-4.382954425133921
2214	20	-2.715742006424378
2214	21	-2.631484261606168
2214	22	-2.564143983831073
2214	23	-2.857327444503092
2214	24	-3.051237691969895
2214	25	-2.8096711603964337
2215	1	-0.8837939504972887
2215	2	-2.5726199218265364
2215	3	-1.1460533866903084
2215	4	-0.8398582038110938
2215	5	-0.7700216653587333
2215	6	-1.2539437415613222
2215	7	-1.6700246604930555
2215	8	-1.6772623856085396
2215	9	-1.37551653691321
2215	10	-2.8906384565971948
2215	11	-1.8255895292714541
2215	12	-2.0625927841089933
2215	13	-1.3842716446559535
2215	14	-2.7511436588426577
2215	15	-2.5710569100157983
2215	16	-2.163057466493882
2215	17	-2.403034953820999
2215	18	-1.3278715264188605
2215	19	-1.9581906578299808
2215	20	-2.2109152373038157
2215	21	-1.8235466905119608
2215	22	-1.543902757687988
2215	23	-2.003499311963168
2215	24	-1.8381654397472857
2215	25	-1.8376795267686816
2216	1	-1.8904351063801883
2216	2	-1.922243969220773
2216	3	-2.3809315227335546
2216	4	-2.997151632364279
2216	5	-2.2100824650269324
2216	6	-2.2971645093728412
2216	7	-2.2027453760194646
2216	8	-2.7507340862343774
2216	9	-2.8845942492377787
2216	10	-2.518702748284717
2216	11	-3.0941142436456133
2216	12	-3.5039551810574316
2216	13	-4.1426252644487995
2216	14	-3.6480973606788325
2216	15	-3.3910914433036936
2216	16	-2.6349911609594656
2216	17	-2.7331338522114947
2216	18	-2.683526670693805
2216	19	-2.9578392701903162
2216	20	-2.896092094704109
2216	21	-3.182491103358153
2216	22	-3.637431051377547
2216	23	-3.646178667833574
2216	24	-3.665558402132369
2216	25	-3.6732969605487042
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	139032.0
3	3.0
4	1138.0
5	6471.0
6	7847.0
7	7149.0
8	6536.0
9	6154.0
10	5691.0
11	5443.0
12	5331.0
13	5301.0
14	5205.0
15	5352.0
16	5544.0
17	5813.0
18	6543.0
19	8006.0
20	10202.0
21	13036.0
22	16435.0
23	20127.0
24	23386.0
25	26429.0
26	29584.0
27	32598.0
28	37692.0
29	45043.0
30	55005.0
31	69152.0
32	87657.0
33	112327.0
34	147152.0
35	194031.0
36	268276.0
37	428327.0
38	1164987.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.62711480624099	19.111475029902316	13.02385658610135	25.23755357775534
2	14.101579834989774	20.014252602991984	42.83268990830073	23.05147765371751
3	18.786567661541607	24.6952445942591	29.718564257521308	26.799623486677987
4	11.970331810560108	16.92604924651269	38.94426145498526	32.159357487941946
5	12.753583838877361	38.35919941873915	36.35495812644946	12.532258615934023
6	31.523363015930638	37.50934978585921	18.486857867989983	12.480429330220169
7	27.66536272879198	31.612816731465344	22.563844063719955	18.157976476022718
8	24.24853563428192	38.276445698166434	20.769036754335023	16.70598191321663
9	26.14320326891672	15.46234377452625	20.571668835347204	37.82278412120983
10	14.59765770316062	29.346442036433	35.18058039395799	20.875319866448393
11	33.808410687713966	22.392185050527576	25.511215887415027	18.288188374343434
12	23.340770187275314	24.64339770974102	32.53947157518938	19.476360527794288
13	28.898455853047363	22.351951082157274	25.9543522404583	22.795240824337064
14	20.18146542339941	22.917551680375528	27.924162732780733	28.976820163444334
15	22.36942413033774	32.359218677140944	23.25144079426636	22.019916398254953
16	21.309768791517456	28.436774994930875	29.02333323709464	21.23012297645703
17	21.060220748236897	29.28894828488529	28.551384081954083	21.099446884923722
18	20.963303971195877	27.889347257098063	30.871464253922703	20.275884517783357
19	23.210020599137206	27.225076852669538	27.709506699282798	21.855395848910455
20	23.177099407892072	28.037249502373147	28.53517503389969	20.250476055835094
21	22.25442158628847	27.38287094132031	27.36757144962186	22.995136022769362
22	22.582599882789502	29.004188649165624	27.51141408556897	20.901797382475902
23	21.63914307731008	28.612840300349053	28.10263452553916	21.645382096801704
24	22.732589877563754	27.35994663843725	28.91396941333807	20.99349407066093
25	22.25977435973679	27.90164378360942	28.4012634686582	21.43731838799559
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	196.0
1	196.0
2	439.5
3	683.0
4	683.0
5	683.0
6	1304.0
7	1925.0
8	1925.0
9	1925.0
10	3147.0
11	4369.0
12	4369.0
13	4369.0
14	8163.0
15	11957.0
16	11957.0
17	11957.0
18	22866.0
19	33775.0
20	33775.0
21	33775.0
22	56798.0
23	79821.0
24	79821.0
25	79821.0
26	116737.0
27	153653.0
28	153653.0
29	153653.0
30	203996.0
31	254339.0
32	254339.0
33	254339.0
34	285879.5
35	317420.0
36	317420.0
37	317420.0
38	339424.0
39	361428.0
40	361428.0
41	361428.0
42	374848.5
43	388269.0
44	388269.0
45	388269.0
46	393824.5
47	399380.0
48	399380.0
49	399380.0
50	376033.5
51	352687.0
52	352687.0
53	352687.0
54	307194.5
55	261702.0
56	261702.0
57	261702.0
58	221384.0
59	181066.0
60	181066.0
61	181066.0
62	146020.5
63	110975.0
64	110975.0
65	110975.0
66	84848.0
67	58721.0
68	58721.0
69	58721.0
70	42512.5
71	26304.0
72	26304.0
73	26304.0
74	18210.0
75	10116.0
76	10116.0
77	10116.0
78	6872.0
79	3628.0
80	3628.0
81	3628.0
82	2385.0
83	1142.0
84	1142.0
85	1142.0
86	725.0
87	308.0
88	308.0
89	308.0
90	205.5
91	103.0
92	103.0
93	103.0
94	59.5
95	16.0
96	16.0
97	16.0
98	19.0
99	22.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3614128045573913
2	0.04054406014588563
3	0.03639675448448161
4	0.0358658993598219
5	0.04127398594229273
6	0.0188121784801286
7	0.018878535370711064
8	0.025049726194880235
9	0.02163234632988333
10	0.021167848095806078
11	0.01994024562003049
12	0.02103513431464115
13	0.020769706752311292
14	0.028832068958080697
15	0.03019238521502121
16	0.022030487673378115
17	0.023490339266192324
18	0.11031833059334674
19	0.025613759764831177
20	0.023324447039736164
21	0.02763764492759634
22	0.020670171416437597
23	0.023589874602066022
24	0.01990706717473926
25	0.023656231492648484
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3014005.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.10207593728374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.56335546861494	55.546338278232824
2	12.337386890109716	16.80403317715802
3	3.284432855417533	6.710300871916637
4	1.1961361646125457	3.258374236550997
5	0.5389839740206646	1.8352963763867132
6	0.2922710862773607	1.1942560627159937
7	0.1679425882523316	0.8006067228784987
8	0.11596447912008237	0.6317937410450724
9	0.08214241162484558	0.5034661878732167
>10	0.3735686708545513	4.478341986466843
>50	0.0251117524878489	1.170753831197371
>100	0.018112820387192804	2.535139269181426
>500	0.002295419111015069	1.0698466801044184
>1k	0.0021000642916167655	2.5573413338102076
>5k	1.9535481782481537E-4	0.9041112444818226
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	7678	0.2547441029460801	No Hit
CTGTAGGACGTGGAATATGGCAAGA	6902	0.22899762940008392	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	6732	0.22335729370057447	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5871	0.19479065230482365	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4453	0.14774361688185655	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3827	0.12697391012954526	No Hit
CTGAAGGACCTGGAATATGGCGAGA	3380	0.1121431450843645	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3118	0.10345039241806168	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.3178445291232096E-5
2	0.0	0.0	0.0	0.0	3.3178445291232096E-5
3	0.0	0.0	0.0	0.0	3.3178445291232096E-5
4	0.0	0.0	0.0	0.0	3.3178445291232096E-5
5	0.0	0.0	0.0	0.0	3.3178445291232096E-5
6	0.0	0.0	0.0	3.3178445291232096E-5	9.953533587369629E-5
7	0.0	0.0	0.0	3.3178445291232096E-5	9.953533587369629E-5
8	3.3178445291232096E-5	0.0	0.0	3.3178445291232096E-5	9.953533587369629E-5
9	3.3178445291232096E-5	0.0	0.0	3.3178445291232096E-5	9.953533587369629E-5
10	3.3178445291232096E-5	0.0	0.0	6.635689058246419E-5	9.953533587369629E-5
11	3.3178445291232096E-5	0.0	0.0	6.635689058246419E-5	9.953533587369629E-5
12	3.3178445291232096E-5	0.0	0.0	6.635689058246419E-5	2.322491170386247E-4
13	3.3178445291232096E-5	0.0	0.0	9.953533587369629E-5	2.322491170386247E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1195	0.0	13.432588	4
TCGGAAC	75	2.0787216E-4	11.397828	3
AGGACGT	3845	0.0	11.338354	5
TCCAACG	320	0.0	11.284347	18
GGACGTG	3735	0.0	11.184658	6
TGTAGGA	3985	0.0	11.130878	2
GGCGAGG	1645	0.0	11.081434	19
GGACCTG	2340	0.0	10.954904	6
AGGACCT	2420	0.0	10.950212	5
GACGTGA	2035	0.0	10.917221	7
CTGTAGG	3945	0.0	10.914482	1
GACGTGG	1875	0.0	10.88674	7
CCAACGA	210	0.0	10.850571	19
GTACTAG	185	5.456968E-12	10.813326	1
GTAGGAC	3865	0.0	10.763797	3
TTAGGAC	2375	0.0	10.637974	3
GTCCTAC	3680	0.0	10.457918	1
TGGCGAG	3875	0.0	10.446738	18
GTCCTAA	1980	0.0	10.440138	1
TAGGACG	4240	0.0	10.282239	4
>>END_MODULE