FastQCFastQC Report
Thu 26 May 2016
SRR1512983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512983_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3014005
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT72850.24170497394662582No Hit
CTGTAGGACGTGGAATATGGCAAGA70860.23510246333367066No Hit
GTCCTAAAGTGTGTATTTCTCATTT63190.2096545957952956No Hit
CTTTAGGACGTGAAATATGGCGAGG62760.20822792264777265No Hit
GTATCAACGCAGAGTACTTTTTTTT44280.14691415574957573No Hit
GTCCTACAGTGTGCATTTCTCATTT41950.13918357799671865No Hit
CTGAAGGACCTGGAATATGGCGAGA35030.11622409385518603No Hit
TATCAACGCAGAGTACTTTTTTTTT31730.10527520690907943No Hit
GTACATGGGAAGCAGTGGTATCAAC31560.10471117333912851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13000.012.8808684
CCAACGT1501.4551915E-1112.02202819
TCCAACG3450.011.82945518
TAAGACG1104.877875E-711.2441464
GGCGAGG15300.011.1659719
ATACCGT600.00582329111.0972226
GGACGGG1303.2103344E-810.9752756
GGACGTG35850.010.6926016
GACGTGG19450.010.6524797
AATGTCC9300.010.627788
TGTAGGA41700.010.6239582
AGGACGT37700.010.599465
AGGACCT24750.010.4176535
GTAGGAC40400.010.3778683
ATAGGAC4500.010.3522433
CTGTAGG40750.010.3365961
GGACCTG23650.010.3364046
TGGCGAG37200.010.28199718
GTCCTAT4300.010.1716361
GTCCTAA21350.010.064961
Produced by FastQC (version 0.11.5)