##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512983_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3014005 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.500634205981743 31.0 31.0 34.0 27.0 34.0 2 30.65708782832145 31.0 31.0 34.0 27.0 34.0 3 30.75801234569949 31.0 31.0 34.0 27.0 34.0 4 34.213809200714664 37.0 35.0 37.0 30.0 37.0 5 33.86729418166194 37.0 35.0 37.0 30.0 37.0 6 33.73565538212445 37.0 35.0 37.0 29.0 37.0 7 33.60876773595266 37.0 35.0 37.0 28.0 37.0 8 33.49980242235829 37.0 35.0 37.0 28.0 37.0 9 34.964367345110574 39.0 35.0 39.0 28.0 39.0 10 34.854924593688466 39.0 35.0 39.0 27.0 39.0 11 34.87862030753101 39.0 35.0 39.0 27.0 39.0 12 34.73220780987424 39.0 35.0 39.0 27.0 39.0 13 34.71279609688769 39.0 35.0 39.0 27.0 39.0 14 35.76605845046707 40.0 36.0 41.0 27.0 41.0 15 35.79451991619125 40.0 36.0 41.0 27.0 41.0 16 35.771626788940296 40.0 36.0 41.0 27.0 41.0 17 35.75404619434938 40.0 36.0 41.0 27.0 41.0 18 35.6812795599211 40.0 36.0 41.0 27.0 41.0 19 35.664446807487046 40.0 36.0 41.0 27.0 41.0 20 35.640362574050144 40.0 36.0 41.0 27.0 41.0 21 35.5887445442194 40.0 36.0 41.0 27.0 41.0 22 35.54720513071478 40.0 36.0 41.0 27.0 41.0 23 35.43372655320744 40.0 36.0 41.0 26.0 41.0 24 35.425620063669435 40.0 36.0 41.0 26.0 41.0 25 35.33268524770198 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.46808932097254896 1101 2 0.4861382884926897 1101 3 0.5167177285254461 1101 4 0.3293556662726118 1101 5 0.46235978253795906 1101 6 0.5714913535279251 1101 7 0.5394815541709264 1101 8 0.5599251881639304 1101 9 0.4592521481776899 1101 10 0.680967649031011 1101 11 0.7188039724016306 1101 12 0.7194588899713708 1101 13 0.8031366220066687 1101 14 0.8793461118015173 1101 15 0.8122017519370957 1101 16 0.9556583808787948 1101 17 0.9060228656975227 1101 18 0.4873476828519401 1101 19 0.7967051136972714 1101 20 0.7678175367103819 1101 21 0.9968715328901325 1101 22 0.8711905092755288 1101 23 0.8157772731124879 1101 24 0.8397628751535393 1101 25 0.9240088279215684 1102 1 0.4425562632625315 1102 2 0.42406772992523756 1102 3 0.430140270608824 1102 4 0.3458200591640548 1102 5 0.5651411671294397 1102 6 0.4573351140337749 1102 7 0.41769369815983026 1102 8 0.40096774140183555 1102 9 0.6852727862350889 1102 10 0.6797824865401836 1102 11 0.6010977233211747 1102 12 0.6195325505736662 1102 13 0.6933949770556183 1102 14 0.7818123482682964 1102 15 0.7665703782892663 1102 16 0.7308944571707201 1102 17 0.7779138683664186 1102 18 0.8848934928870875 1102 19 0.9091163313416857 1102 20 0.8180145103479362 1102 21 0.7786707955084751 1102 22 0.7846190140137779 1102 23 0.8283270134097833 1102 24 0.8572315148610912 1102 25 0.9266059761728016 1103 1 0.4685278231249086 1103 2 0.3959238554911728 1103 3 0.540803130810783 1103 4 0.5517852855777932 1103 5 0.5752965519997773 1103 6 0.5817333470636754 1103 7 0.5882173353538604 1103 8 0.6006449932231064 1103 9 0.6605954732801465 1103 10 0.7639100873782994 1103 11 0.762653661855687 1103 12 0.8288903667208203 1103 13 0.8739618893667966 1103 14 0.8465767513148919 1103 15 0.7240918329170754 1103 16 0.7561421002794759 1103 17 0.8860088320991011 1103 18 1.0268882545776847 1103 19 1.0344038712267078 1103 20 0.9594056945711529 1103 21 0.5634799333562626 1103 22 0.7758506581578573 1103 23 0.8417354151328809 1103 24 0.923237213648143 1103 25 0.9693042265646667 1104 1 0.6495491312713604 1104 2 0.6456137226403307 1104 3 0.5755975836471663 1104 4 0.5803176000744514 1104 5 0.6264315847467827 1104 6 0.7050840544764299 1104 7 0.7153974411107313 1104 8 0.6390880027382693 1104 9 0.8076620757623161 1104 10 0.8395606470560466 1104 11 0.9062737597186015 1104 12 0.8268903187668855 1104 13 0.932190231248839 1104 14 1.0882089741457648 1104 15 1.0088447871561073 1104 16 1.0898559296409331 1104 17 1.0248496748341722 1104 18 1.05911043991226 1104 19 1.0896665389040905 1104 20 1.1637680271243127 1104 21 0.8902586093481233 1104 22 1.146458769381475 1104 23 0.9215552927765742 1104 24 0.9656560654465025 1104 25 1.0811684911080803 1105 1 0.4567796150294754 1105 2 0.601185837510311 1105 3 0.5430420990965601 1105 4 0.4941646070166499 1105 5 0.49913767432236256 1105 6 0.5600956385948663 1105 7 0.6594535226695442 1105 8 0.7170857580425505 1105 9 0.8135142722425641 1105 10 0.7740094217305824 1105 11 0.7542203409220534 1105 12 0.6696905927612633 1105 13 0.7957577078773141 1105 14 1.0113827699188818 1105 15 0.9031763561873944 1105 16 0.939253523323444 1105 17 0.7655376102148708 1105 18 0.9647203415655525 1105 19 0.9075270305595282 1105 20 0.9929023491467319 1105 21 0.9955262017948101 1105 22 1.0242834635152676 1105 23 0.9931217145910125 1105 24 0.9759135347105712 1105 25 1.0792336240371583 1106 1 0.5688812427069863 1106 2 0.6219572839549983 1106 3 0.6252513993414546 1106 4 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0.9268389709382205 1107 17 0.8466588131455737 1107 18 0.9922942673531168 1107 19 1.1632454903466325 1107 20 1.1284038079078158 1107 21 1.0425308558879323 1107 22 1.0283512745547796 1107 23 1.024977456552584 1107 24 1.1059978137743514 1107 25 1.0097243865997712 1108 1 0.36310135787389086 1108 2 0.3436966130904402 1108 3 0.31232162252603857 1108 4 0.3481683302689689 1108 5 0.38110999839940973 1108 6 0.3265686482635175 1108 7 0.24272890385901746 1108 8 0.32773653505538647 1108 9 0.36011442717057207 1108 10 0.45630082243239656 1108 11 0.3390838875319062 1108 12 0.5071744363832167 1108 13 0.4807238090308985 1108 14 0.5678949067578785 1108 15 0.5257770336771301 1108 16 0.46099816314109887 1108 17 0.501468772450977 1108 18 0.5274987531980386 1108 19 0.505069149919052 1108 20 0.6115024340630129 1108 21 0.6502554937074265 1108 22 0.6936618965482424 1108 23 0.6351541639421328 1108 24 0.6573282099978286 1108 25 0.6911251242711032 1109 1 0.006007765862751313 1109 2 0.029769250931419577 1109 3 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2216 15 -2.2814114386750504 2216 16 -2.145634141880386 2216 17 -2.3519392583650287 2216 18 -2.1227690029424764 2216 19 -2.3851338090783187 2216 20 -2.742616214269141 2216 21 -2.5114409388704217 2216 22 -2.8643020131610797 2216 23 -1.9662021988694889 2216 24 -2.2168706211323936 2216 25 -2.0992410061266895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 77137.0 3 0.0 4 1224.0 5 11100.0 6 12877.0 7 9846.0 8 8019.0 9 6928.0 10 6264.0 11 5678.0 12 5270.0 13 4764.0 14 4696.0 15 4657.0 16 4838.0 17 5092.0 18 5621.0 19 6584.0 20 8233.0 21 10850.0 22 13944.0 23 16789.0 24 20226.0 25 23257.0 26 26470.0 27 29304.0 28 33148.0 29 39823.0 30 50455.0 31 64561.0 32 82213.0 33 106844.0 34 139050.0 35 182621.0 36 251833.0 37 395784.0 38 1338005.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.96380527112344 19.714416065059268 13.988293475943783 27.333485187873507 2 12.924435402209156 20.423297381158086 41.68017029838049 24.972096918252266 3 16.972466867175072 24.772752533589028 30.266339969575366 27.988440629660534 4 11.135739937295503 17.4990151603903 38.1497493844545 33.215495517859694 5 12.291022895055669 38.467956650230605 36.56907180349238 12.67194865122135 6 31.550643040906778 37.94346446506673 18.011952339399155 12.493940154627332 7 27.422164220666417 31.667577181934227 22.60933319083714 18.300925406562214 8 24.251522750036603 38.589591683545535 20.41993938546865 16.738946180949217 9 25.813164982997343 15.24549707660571 20.39243020945875 38.54890773093819 10 14.248486883556378 29.744433362262146 35.075738025181685 20.93134172899979 11 33.645875983200405 22.404327707587758 25.419873770771385 18.52992253844046 12 23.098268987778592 24.773337208931927 32.45418962826108 19.674204175028404 13 28.6778070421666 22.26830865768031 26.04424430522293 23.00963999493016 14 19.966401811899917 22.87556825100627 27.85301738339594 29.305012553697875 15 22.241311749117166 32.392980584093074 23.23777178105618 22.12793588573359 16 21.232872621280357 28.568826080204513 28.829378589565422 21.368922708949707 17 20.94791064479667 29.438141692125836 28.433569329796153 21.18037833328134 18 20.887062284275462 28.027741474619365 30.759486405144575 20.3257098359606 19 23.103660393810358 27.190771674798885 27.77552159334399 21.93004633804677 20 23.08361005157444 28.04675410166774 28.436913549770793 20.43272229698702 21 22.062124792385397 27.26607648711114 27.533462198714133 23.13833652178933 22 22.486924335666007 28.931126650466688 27.503951564633795 21.07799744923351 23 21.39745003656266 28.559782933267506 28.207261835087145 21.83550519508269 24 22.704663102413335 27.41252328299716 28.801075778980906 21.081737835608603 25 22.175715975012046 27.89857236327827 28.360892017470867 21.564819644238824 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 126.0 1 126.0 2 300.0 3 474.0 4 474.0 5 474.0 6 1222.5 7 1971.0 8 1971.0 9 1971.0 10 3152.5 11 4334.0 12 4334.0 13 4334.0 14 8080.0 15 11826.0 16 11826.0 17 11826.0 18 23041.0 19 34256.0 20 34256.0 21 34256.0 22 57083.0 23 79910.0 24 79910.0 25 79910.0 26 117665.0 27 155420.0 28 155420.0 29 155420.0 30 204316.5 31 253213.0 32 253213.0 33 253213.0 34 284967.5 35 316722.0 36 316722.0 37 316722.0 38 338644.5 39 360567.0 40 360567.0 41 360567.0 42 375669.5 43 390772.0 44 390772.0 45 390772.0 46 396217.0 47 401662.0 48 401662.0 49 401662.0 50 377851.0 51 354040.0 52 354040.0 53 354040.0 54 307614.5 55 261189.0 56 261189.0 57 261189.0 58 220381.5 59 179574.0 60 179574.0 61 179574.0 62 145102.0 63 110630.0 64 110630.0 65 110630.0 66 84103.0 67 57576.0 68 57576.0 69 57576.0 70 41508.5 71 25441.0 72 25441.0 73 25441.0 74 17478.0 75 9515.0 76 9515.0 77 9515.0 78 6474.5 79 3434.0 80 3434.0 81 3434.0 82 2231.5 83 1029.0 84 1029.0 85 1029.0 86 648.5 87 268.0 88 268.0 89 268.0 90 157.0 91 46.0 92 46.0 93 46.0 94 27.5 95 9.0 96 9.0 97 9.0 98 5.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07800252487968666 2 3.31784452912321E-5 3 0.0 4 0.02733903891997525 5 0.07073644536090683 6 0.07886516445725869 7 0.035135973563414795 8 0.06157919446052678 9 0.0411412721611278 10 0.10504295779204083 11 0.004080948770821548 12 0.009190429345671292 13 0.003417379864996906 14 0.005043123684267279 15 0.004512268559607565 16 0.013569984124113928 17 0.022660878133911523 18 0.0011280671399018912 19 0.0022229558345125506 20 0.003616450536744299 21 2.322491170386247E-4 22 2.986060076210889E-4 23 3.31784452912321E-5 24 9.95353358736963E-5 25 0.002422026506259943 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3014005.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.10107896705779 #Duplication Level Percentage of deduplicated Percentage of total 1 80.72973635905076 54.170524144184206 2 12.711414391946205 17.058992417939542 3 3.5676859600609503 7.181867320071395 4 1.2785164325864307 3.431593284146524 5 0.5894942253542965 1.9777849283061606 6 0.30519871209896804 1.2287497728718313 7 0.17708083901379054 0.8317620753552045 8 0.1280667986001767 0.6874736292742993 9 0.07645346884744401 0.461709922539405 >10 0.3875700334126859 4.568184152182198 >50 0.025567310199476462 1.1701897292314705 >100 0.018659749665443093 2.629614954427978 >500 0.0022778595825409206 1.0569358688507409 >1k 0.0020797848347436734 2.648603068188454 >5k 1.9807474616606412E-4 0.8960147324305284 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 7285 0.24170497394662582 No Hit CTGTAGGACGTGGAATATGGCAAGA 7086 0.23510246333367066 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6319 0.2096545957952956 No Hit CTTTAGGACGTGAAATATGGCGAGG 6276 0.20822792264777265 No Hit GTATCAACGCAGAGTACTTTTTTTT 4428 0.14691415574957573 No Hit GTCCTACAGTGTGCATTTCTCATTT 4195 0.13918357799671865 No Hit CTGAAGGACCTGGAATATGGCGAGA 3503 0.11622409385518603 No Hit TATCAACGCAGAGTACTTTTTTTTT 3173 0.10527520690907943 No Hit GTACATGGGAAGCAGTGGTATCAAC 3156 0.10471117333912851 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.3178445291232096E-5 2 0.0 0.0 0.0 0.0 3.3178445291232096E-5 3 0.0 0.0 0.0 0.0 3.3178445291232096E-5 4 0.0 0.0 0.0 3.3178445291232096E-5 3.3178445291232096E-5 5 0.0 0.0 0.0 3.3178445291232096E-5 3.3178445291232096E-5 6 0.0 0.0 0.0 3.3178445291232096E-5 3.3178445291232096E-5 7 0.0 0.0 0.0 6.635689058246419E-5 3.3178445291232096E-5 8 0.0 0.0 0.0 6.635689058246419E-5 3.3178445291232096E-5 9 0.0 0.0 0.0 9.953533587369629E-5 3.3178445291232096E-5 10 0.0 0.0 0.0 9.953533587369629E-5 3.3178445291232096E-5 11 0.0 0.0 0.0 9.953533587369629E-5 3.3178445291232096E-5 12 0.0 0.0 0.0 9.953533587369629E-5 6.635689058246419E-5 13 0.0 0.0 0.0 9.953533587369629E-5 9.953533587369629E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 1300 0.0 12.880868 4 CCAACGT 150 1.4551915E-11 12.022028 19 TCCAACG 345 0.0 11.829455 18 TAAGACG 110 4.877875E-7 11.244146 4 GGCGAGG 1530 0.0 11.16597 19 ATACCGT 60 0.005823291 11.097222 6 GGACGGG 130 3.2103344E-8 10.975275 6 GGACGTG 3585 0.0 10.692601 6 GACGTGG 1945 0.0 10.652479 7 AATGTCC 930 0.0 10.62778 8 TGTAGGA 4170 0.0 10.623958 2 AGGACGT 3770 0.0 10.59946 5 AGGACCT 2475 0.0 10.417653 5 GTAGGAC 4040 0.0 10.377868 3 ATAGGAC 450 0.0 10.352243 3 CTGTAGG 4075 0.0 10.336596 1 GGACCTG 2365 0.0 10.336404 6 TGGCGAG 3720 0.0 10.281997 18 GTCCTAT 430 0.0 10.171636 1 GTCCTAA 2135 0.0 10.06496 1 >>END_MODULE