FastQCFastQC Report
Thu 26 May 2016
SRR1512982_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512982_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2552749
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC70700.27695633217366844No Hit
CCCATGTACTCTGCGTTGATACCAC59850.2344531326816698No Hit
GTCCTACAGTGGACATTTCTAAATT42210.1653511567333882No Hit
CTGTAGGACGTGGAATATGGCAAGA39330.1540692014765259No Hit
GTCCTAAAGTGTGTATTTCTCATTT38570.15109201883929832No Hit
CTTTAGGACGTGAAATATGGCGAGG31300.12261291650687162No Hit
GAGTACATGGGAAGCAGTGGTATCA29890.11708945924569945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT504.586238E-617.0915186
AGAACCG759.697505E-713.9310635
TAGGACC7100.011.9067434
ATAGGAC3550.011.2378243
GAACCGA600.00589915411.0778366
TCCAACG2500.011.0216618
GTAGGAC19500.011.0086783
TGTAGGA20200.010.7684692
AGGACGT20600.010.7433945
GGACGTG20050.010.7029176
AACACCG803.778146E-410.6857595
CCAACGA1605.493348E-1010.682199519
TAGGACA6500.010.5215674
TTAGGAC12200.010.432933
GACGTGA10900.010.2793037
CTGTAGG21300.010.2400471
TAGGACG22000.010.2326244
TAAGACG750.002651718710.1318814
TAGGACT3850.010.1154334
GGCGAGG10150.010.10336119
Produced by FastQC (version 0.11.5)