##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512982_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2552749
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.451864636907114	31.0	30.0	33.0	18.0	34.0
2	28.579232819207842	31.0	30.0	34.0	16.0	34.0
3	28.72653284753025	31.0	30.0	34.0	20.0	34.0
4	32.19919075475106	35.0	33.0	37.0	22.0	37.0
5	32.28965519132512	35.0	33.0	37.0	23.0	37.0
6	32.42332834132929	35.0	33.0	37.0	23.0	37.0
7	32.31541448062461	35.0	33.0	37.0	22.0	37.0
8	32.32947109175246	35.0	33.0	37.0	22.0	37.0
9	33.63218906363297	38.0	34.0	39.0	21.0	39.0
10	33.47829770964556	38.0	33.0	39.0	19.0	39.0
11	33.557495076875945	38.0	34.0	39.0	19.0	39.0
12	33.43677110440549	38.0	33.0	39.0	18.0	39.0
13	33.443776101763234	38.0	34.0	39.0	18.0	39.0
14	34.39637034428375	39.0	34.0	41.0	17.0	41.0
15	34.31950575634345	39.0	34.0	41.0	17.0	41.0
16	34.212790211650265	39.0	33.0	41.0	17.0	41.0
17	34.140403933171655	39.0	33.0	41.0	17.0	41.0
18	33.84942702944943	39.0	33.0	41.0	11.0	41.0
19	34.06874941484651	39.0	34.0	41.0	17.0	41.0
20	34.137898007207134	39.0	34.0	41.0	16.0	41.0
21	34.06725220536762	39.0	34.0	41.0	11.0	41.0
22	33.974158054708866	39.0	34.0	41.0	10.0	41.0
23	33.70645801839507	39.0	33.0	41.0	2.0	41.0
24	33.69515608467577	39.0	34.0	41.0	2.0	41.0
25	33.60263778381658	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.857005772262827
1101	2	1.1076401461486434
1101	3	1.0893641469031934
1101	4	1.1323527647686262
1101	5	0.9622730390041525
1101	6	1.2024257327570709
1101	7	1.2749223490936927
1101	8	1.1336883197960503
1101	9	0.8889397058050932
1101	10	1.0476781624824838
1101	11	1.231913608379081
1101	12	1.4476754994572758
1101	13	1.289594743435572
1101	14	1.251485972623776
1101	15	1.047533158688978
1101	16	1.568435819793919
1101	17	1.443556159672049
1101	18	1.7274061968079621
1101	19	1.2017078789509057
1101	20	1.4649489502994157
1101	21	1.4362287539527259
1101	22	1.4462149162489126
1101	23	1.6170284704254385
1101	24	1.3590715318738589
1101	25	1.5450456695936055
1102	1	0.7581016680521202
1102	2	0.812644434276482
1102	3	0.8262683026450546
1102	4	0.9817549420824108
1102	5	0.9032011640923372
1102	6	0.8982427530051282
1102	7	1.0853597381330218
1102	8	1.0819507998430709
1102	9	0.9656423502119438
1102	10	1.2348136562797265
1102	11	1.1670947833138356
1102	12	1.175419502519695
1102	13	1.1689144341014455
1102	14	1.2057486416183423
1102	15	1.3473035996851976
1102	16	1.3734751981810192
1102	17	1.3097476764178353
1102	18	1.5969791385372716
1102	19	1.6535407315313293
1102	20	1.5518313279957567
1102	21	1.2392690918007645
1102	22	1.5336895340902217
1102	23	1.7228019223131312
1102	24	1.6487264081316155
1102	25	1.5560879361789617
1103	1	0.31163950155218245
1103	2	0.46583176334669574
1103	3	0.4179234328154209
1103	4	0.6311379307706773
1103	5	0.08378315620034016
1103	6	0.6837914020155296
1103	7	0.4966044517904109
1103	8	0.527713687657382
1103	9	0.7425466170374619
1103	10	0.7791339874350456
1103	11	0.70561374469996
1103	12	0.8070479015007166
1103	13	0.892385158474859
1103	14	1.1007579545205601
1103	15	1.1702732704547003
1103	16	1.238640450230129
1103	17	1.0393528290684486
1103	18	1.2963562278125735
1103	19	1.3633546152650737
1103	20	1.1910475014706918
1103	21	0.9882105016186813
1103	22	1.2306441393690335
1103	23	1.530950970080596
1103	24	1.37363798302016
1103	25	1.3252709407752192
1104	1	0.6803542632020729
1104	2	0.6383575938684274
1104	3	0.7367338166935831
1104	4	1.0379211258171495
1104	5	0.7158144236938639
1104	6	0.8431169589250231
1104	7	1.086774539693522
1104	8	1.1277751324330438
1104	9	1.1930917588431953
1104	10	1.2031349620763123
1104	11	1.2598851076543909
1104	12	1.1559962014523464
1104	13	1.1770403610680589
1104	14	1.3810074077896672
1104	15	1.4846433543610118
1104	16	1.3429867772555042
1104	17	1.2831237806655196
1104	18	1.6020575903119507
1104	19	1.4888294459006914
1104	20	1.4141612588163213
1104	21	1.4286000403085453
1104	22	1.4457086792567324
1104	23	1.7158843354846383
1104	24	1.705369105304655
1104	25	1.3339445979889746
1105	1	0.6880996309505107
1105	2	0.9171535722247413
1105	3	0.8869443920736977
1105	4	1.0426821681278398
1105	5	0.6539881431647956
1105	6	0.5383394441331006
1105	7	0.9523466530208893
1105	8	0.9677925552950768
1105	9	0.8956105391815399
1105	10	1.118754116895353
1105	11	1.081645196400622
1105	12	1.1362400719972783
1105	13	1.1122008073980183
1105	14	1.2348938462446668
1105	15	1.3806750546311832
1105	16	1.2964659905052756
1105	17	1.2955067945069985
1105	18	1.320922743656368
1105	19	1.4509122584996277
1105	20	1.3636419747525679
1105	21	1.417875594262057
1105	22	1.0267435001011052
1105	23	1.329726690891448
1105	24	1.4199870233315934
1105	25	1.4387387031753747
1106	1	0.2884385974051966
1106	2	0.35950302386694943
1106	3	0.3707963871159379
1106	4	0.38512622398702945
1106	5	0.33146861299559305
1106	6	0.3338152731070494
1106	7	0.18441766565452866
1106	8	0.3413562546311937
1106	9	0.5343698969169992
1106	10	0.5987133955646229
1106	11	0.5058512376408331
1106	12	0.614005718707368
1106	13	0.48596713303318495
1106	14	0.6877565967806305
1106	15	0.7254591460040132
1106	16	0.6454403890178781
1106	17	0.6706444354041778
1106	18	0.9089496967928739
1106	19	0.41213267947451016
1106	20	0.5371778461924137
1106	21	0.44888084335214273
1106	22	0.5670498632696805
1106	23	0.8081042914665275
1106	24	0.7056753996748384
1106	25	0.894203586431388
1107	1	0.5846894187964828
1107	2	0.6461743565636873
1107	3	0.7474430238520142
1107	4	0.7977376357131831
1107	5	0.761543345680721
1107	6	0.6846322093893633
1107	7	0.8778338737782292
1107	8	0.7614939845841207
1107	9	0.9721887740567894
1107	10	0.8246348339211522
1107	11	0.8726012597693042
1107	12	1.0600174646760294
1107	13	0.989202110438697
1107	14	1.2362802027308746
1107	15	1.3608416051308225
1107	16	1.260413421591572
1107	17	1.3332099781789566
1107	18	1.7170050466953057
1107	19	1.5302541099761626
1107	20	1.4413698287100374
1107	21	1.4411813661702624
1107	22	1.3867745088770747
1107	23	1.5201127536285952
1107	24	1.3887805606206314
1107	25	1.4178978160879936
1108	1	0.5736809678400903
1108	2	0.6331040375138315
1108	3	0.44691916440403645
1108	4	0.6702767616270933
1108	5	0.6758887968144336
1108	6	0.7704341206241949
1108	7	0.7959291463039264
1108	8	0.8291531092585345
1108	9	1.0062501394655428
1108	10	0.9440086815766904
1108	11	0.7765827981566815
1108	12	0.8473074603894375
1108	13	0.8388742463839236
1108	14	1.0986847227616536
1108	15	0.9366869159604079
1108	16	0.8259678122498997
1108	17	0.8667183904010827
1108	18	1.0890454473969982
1108	19	1.0345541301179821
1108	20	0.8715116305592971
1108	21	0.9468214198129132
1108	22	0.8843662703566224
1108	23	1.0448413985661986
1108	24	1.036292529509268
1108	25	1.2179708086630683
1109	1	-0.01741621377063396
1109	2	0.018431229557531736
1109	3	0.25987635878052373
1109	4	0.4266595979216845
1109	5	0.21076427935506814
1109	6	0.1525677500920537
1109	7	0.2934004892220159
1109	8	0.28455739638239663
1109	9	0.38225114544571426
1109	10	0.47660638306764014
1109	11	0.10054431552425314
1109	12	0.42002887904785524
1109	13	0.32911714072045584
1109	14	0.07683418969651257
1109	15	0.37472601866429756
1109	16	0.33894536322230095
1109	17	0.20923270766720492
1109	18	-1.5186854253173294
1109	19	-0.009319020876141337
1109	20	0.28609218829465277
1109	21	0.3846336110262598
1109	22	0.5697316464929258
1109	23	0.08288541798048499
1109	24	0.4768775331534698
1109	25	0.5166342593059241
1110	1	0.24437827421034797
1110	2	0.20831670045031103
1110	3	0.3511691404316757
1110	4	0.1186598149373097
1110	5	0.33143919795197263
1110	6	0.38949936259820106
1110	7	0.34212583371208893
1110	8	0.23933294085592394
1110	9	0.5285696621261238
1110	10	0.6741080351435613
1110	11	0.5627180472057773
1110	12	0.03559512529122344
1110	13	0.348375854475961
1110	14	0.4746321926191044
1110	15	0.623233280238388
1110	16	0.8387319941703311
1110	17	0.3649261215097752
1110	18	0.7560115310385171
1110	19	0.6873059414351843
1110	20	0.7013928572186359
1110	21	0.794382415439145
1110	22	0.7281158762164139
1110	23	0.6815884827925558
1110	24	0.8665372722188636
1110	25	0.8143258000546112
1111	1	0.2629507467813106
1111	2	0.6516892252083686
1111	3	0.5311468526950094
1111	4	0.41094583507924654
1111	5	0.3863444690588125
1111	6	0.6429258991939548
1111	7	0.436352095423743
1111	8	0.26353885380989794
1111	9	0.68700853453619
1111	10	0.6429329808145781
1111	11	0.602931629498002
1111	12	0.7136889616528208
1111	13	0.7430745957629554
1111	14	0.7311568869443263
1111	15	0.8751666527120392
1111	16	0.7984695916942073
1111	17	0.6887825673222281
1111	18	0.9605059941262226
1111	19	0.690648981688085
1111	20	0.5431281622380695
1111	21	0.7122099292802986
1111	22	0.48584445102478213
1111	23	0.9029520629954959
1111	24	1.0370146488434386
1111	25	0.3456092267745845
1112	1	-0.23504653831323097
1112	2	0.09917373002724261
1112	3	0.19957369065567931
1112	4	0.17446012376558429
1112	5	0.22753664805925666
1112	6	0.11734680107479534
1112	7	-0.1757242450062293
1112	8	0.08630518216631344
1112	9	0.37982436959415367
1112	10	0.3380250574724215
1112	11	0.16329226274136488
1112	12	0.3162145360757904
1112	13	0.0859761858840784
1112	14	0.26427699269117255
1112	15	0.2751792509954143
1112	16	0.3094093984126758
1112	17	0.4430365025787424
1112	18	0.38270055234446687
1112	19	0.2780664584721535
1112	20	0.21451485861076947
1112	21	0.2135525261248361
1112	22	0.29838574685049934
1112	23	0.5814367355659229
1112	24	0.24117530574548596
1112	25	0.43929241181254497
1113	1	-0.2040807113444636
1113	2	-0.07814093428663682
1113	3	-0.3262426521131445
1113	4	-0.13398730054334607
1113	5	-0.24578691659167262
1113	6	-0.15951784606034636
1113	7	-0.2885818473997688
1113	8	0.04667753734665325
1113	9	-0.20193777450673167
1113	10	-0.43476140402920294
1113	11	-0.2553121012414934
1113	12	-0.03976725300437067
1113	13	-0.15861141739516427
1113	14	-0.0745068988131905
1113	15	-0.4338394879393874
1113	16	-0.4982086530807095
1113	17	-0.36466172445893363
1113	18	-0.4708609450962413
1113	19	-0.4232020184017671
1113	20	-0.6094820589497942
1113	21	-0.4478314156991061
1113	22	-0.20197133239457088
1113	23	-0.6590594817056612
1113	24	-0.12291814365705278
1113	25	-0.15518668074142283
1114	1	-1.3514685894702687
1114	2	-1.0914592551399132
1114	3	-0.9140882536385568
1114	4	-1.0693978016655556
1114	5	-1.1944988557340253
1114	6	-1.7002526465449854
1114	7	-1.8457793964096396
1114	8	-1.3947042728903014
1114	9	-1.4727041558204093
1114	10	-1.4934285043529556
1114	11	-2.3178895636523613
1114	12	-1.7370548272391808
1114	13	-1.6243705490216733
1114	14	-1.926835736678406
1114	15	-1.8803626174207864
1114	16	-2.630062338503869
1114	17	-4.684071272197627
1114	18	-3.5831009991213065
1114	19	-2.6198368725142274
1114	20	-1.8134847800652523
1114	21	-2.7889766179951714
1114	22	-2.6948512457955687
1114	23	-2.7277597021756037
1114	24	-2.3144851685046106
1114	25	-2.1975951161993414
1115	1	-0.8761383599862924
1115	2	-0.5662509394526083
1115	3	-0.6153350929747781
1115	4	-0.6133917420777593
1115	5	-0.433548969524864
1115	6	-0.5945722689732342
1115	7	-1.8272350304794074
1115	8	-1.046336987444004
1115	9	-0.606936452979042
1115	10	-0.5169476613426554
1115	11	-1.4300638642795676
1115	12	-1.048108645654274
1115	13	-0.8057289853036664
1115	14	-0.7670865208562745
1115	15	-2.37214516930705
1115	16	-0.8914990467758201
1115	17	-0.9057124290734677
1115	18	-0.9755133232453801
1115	19	-1.0723018778392728
1115	20	-0.7640272131480472
1115	21	-1.613359516183266
1115	22	-1.702759743798154
1115	23	-0.9385896185438511
1115	24	-0.9776447075649273
1115	25	-1.2585098723509205
1116	1	-1.329159502066826
1116	2	-2.268034276529839
1116	3	-1.8519574557642926
1116	4	-1.3951880237592391
1116	5	-2.178084951681388
1116	6	-1.787130380538695
1116	7	-1.516470682576685
1116	8	-2.190724240835543
1116	9	-2.1604251348161085
1116	10	-3.267991065359425
1116	11	-1.8089934644513228
1116	12	-1.9196587943167742
1116	13	-2.546049688122455
1116	14	-2.3454344255664914
1116	15	-1.7858610327588664
1116	16	-1.7963012067057917
1116	17	-2.2482048899265585
1116	18	-1.9493019077411446
1116	19	-2.268366993141342
1116	20	-2.6820714182923595
1116	21	-3.2101375786738657
1116	22	-2.3273741721780823
1116	23	-2.2114819705920254
1116	24	-2.814936465758265
1116	25	-2.5541645340319086
1201	1	0.14800505841961709
1201	2	0.1939186330526077
1201	3	0.34602034177295593
1201	4	0.5779494213408611
1201	5	0.5438379594279255
1201	6	0.6853695970073588
1201	7	0.5176501106763496
1201	8	-0.004141596838380224
1201	9	0.3048700447705599
1201	10	0.585976322528019
1201	11	0.11624364922852237
1201	12	-0.5362929027603585
1201	13	-0.04849177939325955
1201	14	0.6564743844641328
1201	15	0.23440253167049008
1201	16	0.8010002779491785
1201	17	0.9063900857782698
1201	18	1.106466062152606
1201	19	0.6261477321570723
1201	20	0.6896476109786036
1201	21	0.6416942143187043
1201	22	0.8302219980767234
1201	23	1.2085642396308103
1201	24	0.9153399872366421
1201	25	0.8243250338643335
1202	1	0.4402910509413509
1202	2	0.6005842544566207
1202	3	0.3715386049546332
1202	4	0.8836828107101695
1202	5	0.6318099859726516
1202	6	0.716440678888226
1202	7	0.7494993256624483
1202	8	0.7492897870800945
1202	9	1.0576057414489455
1202	10	1.1344132682952761
1202	11	0.7975932255724345
1202	12	0.9081365121677862
1202	13	0.8937318530058036
1202	14	1.0257424253728686
1202	15	0.8678777795106143
1202	16	1.0435282425908525
1202	17	1.1294087486646731
1202	18	1.5606276764587221
1202	19	1.2639540165359264
1202	20	1.1732437598351382
1202	21	1.0651793488591679
1202	22	1.2059200742677163
1202	23	1.3806843013446013
1202	24	1.4796157547393065
1202	25	1.250331972800275
1203	1	0.2694864823314269
1203	2	0.4586673661086209
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2211	17	0.42476135246414515
2211	18	0.46410143551831595
2211	19	0.23935100828169453
2211	20	0.1439286607652761
2211	21	0.18324529744282358
2211	22	0.09992882059253816
2211	23	0.6440830279585867
2211	24	0.45774165548100854
2211	25	0.5350629850517237
2212	1	0.06570908168222545
2212	2	0.013651715378220075
2212	3	0.041161686596012714
2212	4	-0.02958171015563238
2212	5	-0.06643457952356613
2212	6	-0.14420353988723633
2212	7	-0.2494756769473625
2212	8	-0.1509941316024097
2212	9	-0.033784304280153776
2212	10	0.021871566399653375
2212	11	-0.15961911482002478
2212	12	0.008715229193875018
2212	13	-0.11342586886934924
2212	14	-0.13218335415067628
2212	15	-0.15118948119665987
2212	16	0.032915573642156915
2212	17	0.06340771331137063
2212	18	-2.7261060290764654
2212	19	-1.0189946025386902
2212	20	-0.34744699865459694
2212	21	-0.45730649419017766
2212	22	-0.0660656793279557
2212	23	-0.2518646092192327
2212	24	-0.6155341423502918
2212	25	-0.16942772953029106
2213	1	-0.022380312338228237
2213	2	0.054695507690713185
2213	3	-0.3529388972710983
2213	4	-0.4169937444348193
2213	5	-0.24642084711880585
2213	6	-0.21204651353344417
2213	7	-0.0030143981783439244
2213	8	-0.5169380077152006
2213	9	-0.29319540387295717
2213	10	-0.33988457401730443
2213	11	-0.3484607922366223
2213	12	-0.36376137545705944
2213	13	-0.12870148979644114
2213	14	-0.21071908251392557
2213	15	-0.34460130963372393
2213	16	-1.0809105636535818
2213	17	-0.4602995177689664
2213	18	-0.3440733596759529
2213	19	-0.256428216653795
2213	20	-0.8572174223079188
2213	21	-0.6100874141125558
2213	22	-0.4190861246330897
2213	23	-0.8076157974297047
2213	24	-0.5318721533539659
2213	25	-0.7014738458435659
2214	1	-1.5333602420247416
2214	2	-3.765919471908493
2214	3	-1.757485193825218
2214	4	-1.456072073785517
2214	5	-1.5029070264646087
2214	6	-1.6424380432851677
2214	7	-1.7388421177145155
2214	8	-1.4186976358822712
2214	9	-1.5445316751926086
2214	10	-1.5539116611687191
2214	11	-1.3868423401033496
2214	12	-1.9714857538018187
2214	13	-1.7351797666205364
2214	14	-2.970200833963734
2214	15	-3.130083926242982
2214	16	-2.3511655634529163
2214	17	-2.8366602842913515
2214	18	-2.1527295819411414
2214	19	-4.47218673389234
2214	20	-2.7267215583531303
2214	21	-2.632848899012725
2214	22	-2.8106601278597267
2214	23	-2.851659773361593
2214	24	-2.9453771250140477
2214	25	-2.7571994352546874
2215	1	-0.9300378672841063
2215	2	-2.727796400252963
2215	3	-1.3800308159842416
2215	4	-0.9976616241066587
2215	5	-0.9237533171221912
2215	6	-1.5356065130813938
2215	7	-1.8723617087703772
2215	8	-1.9065103070558038
2215	9	-1.6214823343294675
2215	10	-3.2865435469427347
2215	11	-2.2937435339030827
2215	12	-2.552091729460006
2215	13	-1.786967153883534
2215	14	-3.2704877517470905
2215	15	-3.0928019780053333
2215	16	-2.578571528930034
2215	17	-2.929924707148679
2215	18	-1.9688386086068164
2215	19	-2.5219713799381616
2215	20	-2.550211021124074
2215	21	-2.1427795875700753
2215	22	-1.8496488690650423
2215	23	-2.4888240117394
2215	24	-2.289730904508197
2215	25	-2.1195955979690417
2216	1	-1.908223993222954
2216	2	-1.9929379601403276
2216	3	-2.4520304868728253
2216	4	-3.1867560303879827
2216	5	-2.249334509412254
2216	6	-2.34213165064493
2216	7	-2.3047254507671795
2216	8	-2.991027629902348
2216	9	-3.248974219886012
2216	10	-2.890707142182709
2216	11	-3.4553856823289735
2216	12	-3.769067665706423
2216	13	-4.3140519319768025
2216	14	-3.5733916562510473
2216	15	-3.431530499707378
2216	16	-2.7214730490553656
2216	17	-2.7567644987338404
2216	18	-2.9199685018303114
2216	19	-3.267554654361671
2216	20	-2.96687119511655
2216	21	-3.3949418159901903
2216	22	-3.967474450089423
2216	23	-3.8529895715730333
2216	24	-3.904326240193445
2216	25	-4.03607534838169
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	138538.0
3	4.0
4	1071.0
5	6420.0
6	7902.0
7	6976.0
8	6165.0
9	5891.0
10	5618.0
11	5422.0
12	5129.0
13	4987.0
14	5257.0
15	5263.0
16	5466.0
17	5751.0
18	6427.0
19	7992.0
20	10070.0
21	12800.0
22	16202.0
23	19771.0
24	22735.0
25	25651.0
26	28797.0
27	32152.0
28	36430.0
29	43003.0
30	53000.0
31	64902.0
32	81546.0
33	102629.0
34	133045.0
35	171801.0
36	232939.0
37	367893.0
38	867104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52089620067649	18.44863970663392	12.566104077656467	25.464360015033126
2	14.882651719615028	19.739685856405945	41.17984311238936	24.197819311589676
3	19.162892620634604	24.093807690785425	29.17277169310687	27.570527995473103
4	12.405696237254395	16.616232865439326	37.765198717798	33.21287217950828
5	13.395050673246226	37.77279904190903	35.697314108289206	13.134836176555536
6	32.669576991974274	36.409940555390996	17.988694083592314	12.931788369042414
7	28.407593151275318	31.24436782509893	21.874074364298867	18.47396465932688
8	25.312963460005054	36.75620607844021	20.434263861433664	17.496566600121074
9	26.617746914729096	15.066293603927894	19.7990993677237	38.51686011361931
10	14.962424869329446	28.861618512510677	34.60877197106832	21.56718464709155
11	34.496030178790846	22.22081170540886	24.35410084254871	18.929057273251587
12	23.49326804138657	24.502934493730773	31.482950785372317	20.520846679510342
13	29.145538051880987	21.56239298557416	25.42968373490435	23.862385227640498
14	21.033286821359145	22.03699719948543	27.28756037838098	29.642155600774444
15	23.613796886785984	30.365415699297614	22.90988528717391	23.110902126742488
16	22.431958648976295	27.86206242619107	27.645778266419768	22.060200658412864
17	22.213985242397076	28.24260552563773	27.52846982339776	22.014939408567436
18	22.13576587385607	27.36043475192321	29.084660565710745	21.41913880850997
19	23.7709562220378	26.97885589391663	27.10584905172434	22.14433883232123
20	23.796142106521117	27.298220729810318	27.475129007863895	21.430508155804667
21	23.087131928259414	26.80613177309211	26.903777461870376	23.2029588367781
22	23.27622280628341	27.8894180963889	26.573556446797408	22.26080265053028
23	22.550640299008645	27.60117186688802	27.065352698314804	22.782835135788527
24	23.212985753097044	27.09885380644868	27.561617600618117	22.126542839836162
25	23.097108027371238	26.970951755527068	27.14958419665163	22.782356020450063
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	139.0
1	139.0
2	296.5
3	454.0
4	454.0
5	454.0
6	782.0
7	1110.0
8	1110.0
9	1110.0
10	1878.5
11	2647.0
12	2647.0
13	2647.0
14	5189.0
15	7731.0
16	7731.0
17	7731.0
18	15690.5
19	23650.0
20	23650.0
21	23650.0
22	39575.5
23	55501.0
24	55501.0
25	55501.0
26	81714.0
27	107927.0
28	107927.0
29	107927.0
30	143590.5
31	179254.0
32	179254.0
33	179254.0
34	206748.5
35	234243.0
36	234243.0
37	234243.0
38	256466.0
39	278689.0
40	278689.0
41	278689.0
42	297225.5
43	315762.0
44	315762.0
45	315762.0
46	335326.0
47	354890.0
48	354890.0
49	354890.0
50	339576.5
51	324263.0
52	324263.0
53	324263.0
54	288979.5
55	253696.0
56	253696.0
57	253696.0
58	218364.0
59	183032.0
60	183032.0
61	183032.0
62	149842.0
63	116652.0
64	116652.0
65	116652.0
66	90427.0
67	64202.0
68	64202.0
69	64202.0
70	47223.0
71	30244.0
72	30244.0
73	30244.0
74	21110.5
75	11977.0
76	11977.0
77	11977.0
78	8231.5
79	4486.0
80	4486.0
81	4486.0
82	3020.5
83	1555.0
84	1555.0
85	1555.0
86	1011.0
87	467.0
88	467.0
89	467.0
90	300.0
91	133.0
92	133.0
93	133.0
94	78.5
95	24.0
96	24.0
97	24.0
98	22.5
99	21.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3538538258167959
2	0.03862502737245221
3	0.03486437562016477
4	0.034825202164411775
5	0.03948684339901808
6	0.017902269279118314
7	0.017588881633094362
8	0.024052501832338393
9	0.021232013018122817
10	0.02072275809333389
11	0.019312513686226104
12	0.020370196991556944
13	0.020487717358815927
14	0.028126541230649782
15	0.028361581965167745
16	0.021584574119899762
17	0.022446390146465635
18	0.09123497844872332
19	0.02518853204917522
20	0.022681430880983598
21	0.02605034807574109
22	0.019782595155262032
23	0.02279895124824258
24	0.01860739148267221
25	0.022759777792489586
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2552749.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.965404173238
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.29948241797791	54.57586777439688
2	12.7774312777131	17.368465621710875
3	3.6898722593130007	7.5235097835548075
4	1.4151601098562532	3.8472771534489656
5	0.6563975045968178	2.2306160849113783
6	0.33367124779758917	1.3606860730531076
7	0.19826747679041581	0.9432730436128085
8	0.12483025295902452	0.6787310876326111
9	0.08786895370741854	0.5374844057703817
>10	0.3785274015753946	4.340602978414387
>50	0.020258404156313862	0.9507086879962493
>100	0.014073095695763189	1.9531532646089436
>500	0.0024264320856068485	1.1249844987742066
>1k	0.0016176213903814586	2.052033607545484
>5k	1.1554438502724704E-4	0.5126059345689192
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	7070	0.27695633217366844	No Hit
CCCATGTACTCTGCGTTGATACCAC	5985	0.2344531326816698	No Hit
GTCCTACAGTGGACATTTCTAAATT	4221	0.1653511567333882	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3933	0.1540692014765259	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3857	0.15109201883929832	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3130	0.12261291650687162	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2989	0.11708945924569945	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.917345575299413E-5	0.0	0.0	0.0
6	0.0	3.917345575299413E-5	0.0	0.0	0.0
7	0.0	3.917345575299413E-5	0.0	0.0	0.0
8	3.917345575299413E-5	3.917345575299413E-5	0.0	0.0	0.0
9	3.917345575299413E-5	3.917345575299413E-5	0.0	0.0	0.0
10	3.917345575299413E-5	3.917345575299413E-5	0.0	0.0	3.917345575299413E-5
11	3.917345575299413E-5	3.917345575299413E-5	0.0	0.0	3.917345575299413E-5
12	3.917345575299413E-5	3.917345575299413E-5	0.0	0.0	3.917345575299413E-5
13	3.917345575299413E-5	7.834691150598825E-5	0.0	0.0	3.917345575299413E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACCGT	50	4.586238E-6	17.091518	6
AGAACCG	75	9.697505E-7	13.931063	5
TAGGACC	710	0.0	11.906743	4
ATAGGAC	355	0.0	11.237824	3
GAACCGA	60	0.005899154	11.077836	6
TCCAACG	250	0.0	11.02166	18
GTAGGAC	1950	0.0	11.008678	3
TGTAGGA	2020	0.0	10.768469	2
AGGACGT	2060	0.0	10.743394	5
GGACGTG	2005	0.0	10.702917	6
AACACCG	80	3.778146E-4	10.685759	5
CCAACGA	160	5.493348E-10	10.6821995	19
TAGGACA	650	0.0	10.521567	4
TTAGGAC	1220	0.0	10.43293	3
GACGTGA	1090	0.0	10.279303	7
CTGTAGG	2130	0.0	10.240047	1
TAGGACG	2200	0.0	10.232624	4
TAAGACG	75	0.0026517187	10.131881	4
TAGGACT	385	0.0	10.115433	4
GGCGAGG	1015	0.0	10.103361	19
>>END_MODULE