FastQCFastQC Report
Thu 26 May 2016
SRR1512982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512982_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2552749
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC70690.27691715871791545No Hit
GTACATGGGAAGCAGTGGTATCAAC69460.2720988236602972No Hit
CTGTAGGACGTGGAATATGGCAAGA41790.16370587159176245No Hit
GTCCTACAGTGGACATTTCTAAATT40880.16014108711824No Hit
GTCCTAAAGTGTGTATTTCTCATTT36620.14345319496746448No Hit
CTTTAGGACGTGAAATATGGCGAGG33820.13248462735662614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG400.00522697914.2718295
GGCGAGG10400.012.31892919
TAGGACC7650.011.9398314
GTTCCGT657.9410925E-411.707236
AGGACGT22350.011.2812365
GGACGTG21800.010.9520326
TGGCGAG21900.010.70369818
GTAGGAC21500.010.6117273
TTAGGAC13850.010.5702243
TGTAGGA22550.010.4127062
TAGGACG24350.010.393724
GACGTGA11800.010.3101397
ATAGGAC2500.010.2668443
TAGGACA5850.010.2464414
CTGTAGG22650.010.2404211
GAACAGT6700.010.2219836
ACACCGT856.5191404E-410.0716616
GACGTGG12300.09.9683017
ATGGCGA24250.09.8250917
GATATAC6100.09.8176361
Produced by FastQC (version 0.11.5)