##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512982_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2552749 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.675945421974507 31.0 31.0 34.0 27.0 34.0 2 30.792138984287135 31.0 31.0 34.0 27.0 34.0 3 30.9116951960416 31.0 31.0 34.0 27.0 34.0 4 34.4147971461354 37.0 35.0 37.0 31.0 37.0 5 34.10767255221724 37.0 35.0 37.0 30.0 37.0 6 33.99311879076243 37.0 35.0 37.0 30.0 37.0 7 33.87452115347024 37.0 35.0 37.0 29.0 37.0 8 33.764445701477115 37.0 35.0 37.0 29.0 37.0 9 35.24745676131888 39.0 35.0 39.0 29.0 39.0 10 35.13606390600878 39.0 35.0 39.0 28.0 39.0 11 35.18565162497371 39.0 35.0 39.0 29.0 39.0 12 35.040743136125016 39.0 35.0 39.0 27.0 39.0 13 35.04115132353396 39.0 35.0 39.0 27.0 39.0 14 36.12095509585941 40.0 36.0 41.0 27.0 41.0 15 36.13731824006199 40.0 36.0 41.0 27.0 41.0 16 36.107406172718115 40.0 36.0 41.0 27.0 41.0 17 36.077369925519506 40.0 36.0 41.0 27.0 41.0 18 35.99951464088322 40.0 36.0 41.0 27.0 41.0 19 35.972137096126566 40.0 36.0 41.0 27.0 41.0 20 35.95430455559869 40.0 36.0 41.0 27.0 41.0 21 35.89948757202529 40.0 36.0 41.0 27.0 41.0 22 35.86642047455508 40.0 36.0 41.0 27.0 41.0 23 35.77211547237899 40.0 36.0 41.0 27.0 41.0 24 35.755123202477016 40.0 36.0 41.0 27.0 41.0 25 35.66279136726721 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5714313022800575 1101 2 0.5920833330638864 1101 3 0.5621096732374156 1101 4 0.44944310672203613 1101 5 0.7242366002413831 1101 6 0.660533211145399 1101 7 0.7530248397351613 1101 8 0.6539376168844129 1101 9 0.482435396547956 1101 10 0.872695999202044 1101 11 0.8728437617143427 1101 12 0.9939833010892869 1101 13 0.9923686704614099 1101 14 1.1134382133153977 1101 15 1.1010982650856818 1101 16 1.2457909747216647 1101 17 1.1340051239628082 1101 18 0.6465887811884627 1101 19 1.023493148198213 1101 20 0.9896388012835828 1101 21 1.0972729247355772 1101 22 1.078540039699142 1101 23 1.0463114834982647 1101 24 0.9796957791579075 1101 25 0.9432338410983618 1102 1 0.6101163500261215 1102 2 0.585540840109541 1102 3 0.6054214820912023 1102 4 0.5345708487620016 1102 5 0.6913366602383704 1102 6 0.5864158354550923 1102 7 0.5911192423480358 1102 8 0.6379866401850833 1102 9 0.9109827947770057 1102 10 0.8123319193582077 1102 11 0.8654905120005267 1102 12 0.8983082368900597 1102 13 1.0189270889949569 1102 14 1.1100601010486528 1102 15 1.038294921450209 1102 16 0.9704892414346276 1102 17 1.0807437542893794 1102 18 1.0328119282448682 1102 19 1.0676941147753567 1102 20 1.1228146783253479 1102 21 1.1072673912716198 1102 22 1.0868936071883866 1102 23 1.149989580673747 1102 24 1.1772033892045215 1102 25 1.280031008357433 1103 1 0.6675357809862916 1103 2 0.5745833113171628 1103 3 0.6204369899777937 1103 4 0.6259443450424911 1103 5 0.6684541469875143 1103 6 0.7625976584866407 1103 7 0.6784617935041126 1103 8 0.7313456313590336 1103 9 0.8347593754772475 1103 10 0.9026812487114952 1103 11 0.8123222629054396 1103 12 0.8899707203002123 1103 13 0.8823250403246803 1103 14 1.0220812303188538 1103 15 0.7660313659569198 1103 16 0.9761940098183928 1103 17 1.035391446393369 1103 18 1.056332803636046 1103 19 1.042699107048449 1103 20 1.1313232899063195 1103 21 0.8373320012579413 1103 22 1.0414618794966373 1103 23 1.0580119652813451 1103 24 1.1173397269210383 1103 25 1.200377639119786 1104 1 0.5537390692930266 1104 2 0.5627746944578433 1104 3 0.5387613052309064 1104 4 0.4958438207153435 1104 5 0.5888564369129554 1104 6 0.6452716041441349 1104 7 0.6541738919852591 1104 8 0.5974406834414268 1104 9 0.8250680993593065 1104 10 0.831920318246901 1104 11 0.8874695606234368 1104 12 0.798041679385264 1104 13 0.9942890026748259 1104 14 1.0440366621501838 1104 15 1.0970944689229043 1104 16 1.0588673294711128 1104 17 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-1.038858646170027 2213 22 -0.4330477423557326 2213 23 -0.6198927366199243 2213 24 -0.7203960226859252 2213 25 -0.7422599585044978 2214 1 -0.8621206779751027 2214 2 -0.8258186722218959 2214 3 -0.7076764221374923 2214 4 -1.157556167093169 2214 5 -1.1569107585499694 2214 6 -2.471361075871066 2214 7 -1.09940141557189 2214 8 -1.7424844884183202 2214 9 -1.1384904659861164 2214 10 -1.3518659979182814 2214 11 -1.1440626910410998 2214 12 -1.275039014088108 2214 13 -1.3868890248879069 2214 14 -1.5651128204591203 2214 15 -1.8552741879085275 2214 16 -1.4120137969918218 2214 17 -1.4213828572500447 2214 18 -1.3801460980412728 2214 19 -3.1667492629727647 2214 20 -1.7869192806387701 2214 21 -2.2206491314293046 2214 22 -1.9086837907706737 2214 23 -1.8768437201995383 2214 24 -1.2570537227030627 2214 25 -1.0140680097165031 2215 1 -1.5227054825945174 2215 2 -1.3141521816318864 2215 3 -1.2223037201557752 2215 4 -1.1463505789430926 2215 5 -1.7353393807344446 2215 6 -1.741978972441892 2215 7 -1.9271004004986843 2215 8 -2.6973736490347484 2215 9 -2.0878363150573875 2215 10 -1.538636330152272 2215 11 -2.07590031419452 2215 12 -2.9035964162984484 2215 13 -2.3074502108286907 2215 14 -2.3221630169502063 2215 15 -3.4636640836885775 2215 16 -2.8908667813516757 2215 17 -2.6221306077400115 2215 18 -3.3033033860981433 2215 19 -3.923244870296017 2215 20 -3.5271629777046343 2215 21 -2.593504108070384 2215 22 -3.4856008035761548 2215 23 -3.2527191959987647 2215 24 -2.3673742903044683 2215 25 -2.5446493172094478 2216 1 -1.217273549048155 2216 2 -1.0627154255957976 2216 3 -1.585702416807404 2216 4 -0.9711408123939052 2216 5 -0.8330302275750583 2216 6 -1.3463419655423081 2216 7 -2.6933349964892344 2216 8 -1.7986837139670868 2216 9 -1.877606659096358 2216 10 -1.8269641251225721 2216 11 -2.4216504111648476 2216 12 -1.7206016535357236 2216 13 -2.2112535466079706 2216 14 -2.3036703976095367 2216 15 -2.3491124895971325 2216 16 -2.1274789151522597 2216 17 -2.2788547233790126 2216 18 -2.0149722328480877 2216 19 -2.263290929102851 2216 20 -2.751122456701111 2216 21 -2.51587184642009 2216 22 -2.8443820334789507 2216 23 -1.8681338679871828 2216 24 -2.285304649657192 2216 25 -2.0605111441472204 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52030.0 3 1.0 4 791.0 5 7368.0 6 8648.0 7 6670.0 8 5575.0 9 4891.0 10 4359.0 11 4012.0 12 3810.0 13 3546.0 14 3486.0 15 3588.0 16 3615.0 17 4002.0 18 4425.0 19 5212.0 20 6570.0 21 8608.0 22 11268.0 23 13987.0 24 16673.0 25 19741.0 26 22886.0 27 25398.0 28 29168.0 29 34958.0 30 45005.0 31 57489.0 32 73525.0 33 94594.0 34 123830.0 35 161203.0 36 220226.0 37 340238.0 38 1121353.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.7780037667146 19.21538017629228 13.515950515218186 27.490665541774934 2 13.706366629216829 20.19560881058373 39.919608202611464 26.17841635758798 3 17.383103470023883 24.128145775397424 29.807004135541725 28.681746619036968 4 11.67429388924873 17.16593333262802 37.052745584615934 34.10702719350732 5 12.982863827128263 37.88119693701788 35.93484538086961 13.201093854984244 6 32.677643830246005 36.81438792512006 17.61183965500343 12.896128589630502 7 28.208119440416944 31.243034601669343 21.94086758885536 18.607978369058348 8 25.320344219614434 37.15600969283801 20.12604452402474 17.39760156352282 9 26.22876227860881 14.900148613101155 19.70021069199151 39.170878416298535 10 14.593777843311958 29.120574874195288 34.6495899582115 21.63605732428125 11 34.50056960012034 22.14671081394055 24.237699536328265 19.11502004961084 12 23.244511552029188 24.533938346343046 31.62163284559318 20.59991725603459 13 28.956529165331457 21.416690236906373 25.59159995831789 24.03518063944428 14 20.819494621608683 21.924828666367365 27.31638291921013 29.93929379281382 15 23.462433318538086 30.42672609839063 22.954719874199892 23.15612070887139 16 22.3490808506637 27.89054051936247 27.542979830431445 22.217398799542384 17 22.055214543630697 28.391740741248682 27.5396005948171 22.01344412030352 18 22.1064247612265 27.419005434567577 29.06872751674382 21.405842287462104 19 23.662539913730324 26.984991928132366 27.12468753586898 22.227780622268327 20 23.67052408076281 27.26450056019995 27.505739111358345 21.559236247678893 21 22.93602815475715 26.701029635514356 26.987975311311573 23.374966898416922 22 23.11557828315077 27.79951471865983 26.615652321069156 22.469254677120247 23 22.280185901624446 27.51066693618015 27.22752108707949 22.981626075115912 24 23.175887318293853 27.124103123379196 27.49969836427254 22.200311194054407 25 22.9210607138492 26.991776537433367 27.193406500421514 22.89375624829592 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 57.0 1 57.0 2 158.5 3 260.0 4 260.0 5 260.0 6 684.0 7 1108.0 8 1108.0 9 1108.0 10 1726.5 11 2345.0 12 2345.0 13 2345.0 14 4840.5 15 7336.0 16 7336.0 17 7336.0 18 15361.5 19 23387.0 20 23387.0 21 23387.0 22 39814.0 23 56241.0 24 56241.0 25 56241.0 26 82948.5 27 109656.0 28 109656.0 29 109656.0 30 144678.5 31 179701.0 32 179701.0 33 179701.0 34 206875.0 35 234049.0 36 234049.0 37 234049.0 38 257364.0 39 280679.0 40 280679.0 41 280679.0 42 298653.0 43 316627.0 44 316627.0 45 316627.0 46 336631.5 47 356636.0 48 356636.0 49 356636.0 50 342561.5 51 328487.0 52 328487.0 53 328487.0 54 290204.5 55 251922.0 56 251922.0 57 251922.0 58 215970.5 59 180019.0 60 180019.0 61 180019.0 62 147684.0 63 115349.0 64 115349.0 65 115349.0 66 89175.0 67 63001.0 68 63001.0 69 63001.0 70 45801.5 71 28602.0 72 28602.0 73 28602.0 74 19955.5 75 11309.0 76 11309.0 77 11309.0 78 7753.0 79 4197.0 80 4197.0 81 4197.0 82 2749.0 83 1301.0 84 1301.0 85 1301.0 86 839.5 87 378.0 88 378.0 89 378.0 90 234.5 91 91.0 92 91.0 93 91.0 94 51.0 95 11.0 96 11.0 97 11.0 98 5.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07775930966969334 2 3.917345575299412E-5 3 0.0 4 0.027656459761613853 5 0.0693761901385526 6 0.07830773805023526 7 0.033258263934292015 8 0.06216827428000167 9 0.04532368830621421 10 0.10831460515702876 11 0.0031730499159925243 12 0.011634516358639256 13 0.0037214782965344423 14 0.004975028880630254 15 0.005014202336383247 16 0.015238474287914715 17 0.027578112850107864 18 6.267752920479059E-4 19 0.002193713522167671 20 0.003486437562016477 21 3.917345575299412E-5 22 1.1752036725898237E-4 23 3.917345575299412E-5 24 3.917345575299412E-5 25 0.0020761931549086884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2552749.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.8919171557449 #Duplication Level Percentage of deduplicated Percentage of total 1 78.33830192293317 51.618609004276436 2 13.882616374729148 18.295044161372815 3 4.165445868680107 8.234076422874285 4 1.5919880577876868 4.19596580866726 5 0.7397507448271042 2.437179739702407 6 0.3754510634543228 1.4843514221501135 7 0.22230652723533376 1.025374229304037 8 0.14013262315341032 0.7386885756513387 9 0.10289462857154733 0.6101931907446784 >10 0.39934398570093105 4.457524139360245 >50 0.022599303796146216 1.0451352192648689 >100 0.014949036207354249 2.0481932283369746 >500 0.002436822251757913 1.157413137841462 >1k 0.0016641712938565906 2.103387095472983 >5k 1.188693781326136E-4 0.5488646249800565 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 7069 0.27691715871791545 No Hit GTACATGGGAAGCAGTGGTATCAAC 6946 0.2720988236602972 No Hit CTGTAGGACGTGGAATATGGCAAGA 4179 0.16370587159176245 No Hit GTCCTACAGTGGACATTTCTAAATT 4088 0.16014108711824 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3662 0.14345319496746448 No Hit CTTTAGGACGTGAAATATGGCGAGG 3382 0.13248462735662614 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 3.917345575299413E-5 0.0 0.0 0.0 4 0.0 3.917345575299413E-5 0.0 0.0 0.0 5 0.0 3.917345575299413E-5 0.0 0.0 0.0 6 0.0 3.917345575299413E-5 0.0 0.0 0.0 7 0.0 3.917345575299413E-5 0.0 0.0 0.0 8 0.0 3.917345575299413E-5 0.0 0.0 0.0 9 0.0 3.917345575299413E-5 0.0 0.0 0.0 10 0.0 3.917345575299413E-5 0.0 0.0 0.0 11 0.0 3.917345575299413E-5 0.0 0.0 0.0 12 0.0 3.917345575299413E-5 0.0 0.0 0.0 13 0.0 3.917345575299413E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATACCG 40 0.005226979 14.271829 5 GGCGAGG 1040 0.0 12.318929 19 TAGGACC 765 0.0 11.939831 4 GTTCCGT 65 7.9410925E-4 11.70723 6 AGGACGT 2235 0.0 11.281236 5 GGACGTG 2180 0.0 10.952032 6 TGGCGAG 2190 0.0 10.703698 18 GTAGGAC 2150 0.0 10.611727 3 TTAGGAC 1385 0.0 10.570224 3 TGTAGGA 2255 0.0 10.412706 2 TAGGACG 2435 0.0 10.39372 4 GACGTGA 1180 0.0 10.310139 7 ATAGGAC 250 0.0 10.266844 3 TAGGACA 585 0.0 10.246441 4 CTGTAGG 2265 0.0 10.240421 1 GAACAGT 670 0.0 10.221983 6 ACACCGT 85 6.5191404E-4 10.071661 6 GACGTGG 1230 0.0 9.968301 7 ATGGCGA 2425 0.0 9.82509 17 GATATAC 610 0.0 9.817636 1 >>END_MODULE