FastQCFastQC Report
Thu 26 May 2016
SRR1512981_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512981_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3372106
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT65980.1956640746168715No Hit
CTGTAGGACGTGGAATATGGCAAGA60500.17941310267233593No Hit
GTCCTAAAGTGTGTATTTCTCATTT57690.17108003129201751No Hit
CTTTAGGACGTGAAATATGGCGAGG51410.15245665468404612No Hit
GTACATGGGAAGCAGTGGTATCAAC46150.13685809402195542No Hit
CCCATGTACTCTGCGTTGATACCAC39140.11606989815859882No Hit
GTCCTACAGTGTGCATTTCTCATTT36850.1092788898095137No Hit
GTATCAACGCAGAGTACTTTTTTTT36630.10862647852706883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACA655.4681834E-513.1507642
TAGGACC11500.013.1317054
CCGTGTA1207.4942363E-1012.6591929
TGTACCG1600.012.4652745
TCTATAC3200.011.8722173
GTAGGAC34250.011.3141373
AGGACCT23100.011.2652585
TGTAGGA35350.011.2307492
GTCCTAA17700.011.0910981
CTGTAGG35050.011.0115271
ATACCGT951.370778E-510.9931816
AGGACGT33450.010.6756485
GGACCTG23500.010.5041256
GACGTGG17000.010.4993727
GGACGTG32650.010.4973186
GTCCTAC31250.010.1853741
TAGGACG35800.010.1079674
GCCTTAC2002.3646862E-1110.0062161
TGGCAAG26700.09.96538818
TTAGGAC20950.09.747123
Produced by FastQC (version 0.11.5)