##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512981_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3372106 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.91477462452248 31.0 30.0 33.0 23.0 34.0 2 29.033498353847715 31.0 30.0 34.0 22.0 34.0 3 29.18778828423543 31.0 30.0 34.0 23.0 34.0 4 32.673226167860676 35.0 33.0 37.0 25.0 37.0 5 32.76960214180693 35.0 33.0 37.0 26.0 37.0 6 32.8789513141046 37.0 35.0 37.0 26.0 37.0 7 32.78247421640957 37.0 35.0 37.0 25.0 37.0 8 32.789468065357376 37.0 35.0 37.0 25.0 37.0 9 34.13133839802189 39.0 35.0 39.0 25.0 39.0 10 33.98678867153049 39.0 34.0 39.0 25.0 39.0 11 34.069347760716894 39.0 35.0 39.0 25.0 39.0 12 33.94928925721789 39.0 34.0 39.0 24.0 39.0 13 33.9550740694391 39.0 35.0 39.0 24.0 39.0 14 34.96498538302177 39.0 35.0 41.0 24.0 41.0 15 34.90080679551592 39.0 35.0 41.0 23.0 41.0 16 34.78169102632005 39.0 34.0 41.0 22.0 41.0 17 34.73265727708441 39.0 34.0 41.0 21.0 41.0 18 34.412364854485595 39.0 34.0 41.0 17.0 41.0 19 34.65206224240875 39.0 34.0 41.0 18.0 41.0 20 34.729649957622925 39.0 34.0 41.0 19.0 41.0 21 34.6627748356665 39.0 34.0 41.0 18.0 41.0 22 34.59151432368971 39.0 34.0 41.0 18.0 41.0 23 34.317374364862786 39.0 34.0 41.0 16.0 41.0 24 34.317216896503254 39.0 34.0 41.0 16.0 41.0 25 34.24169821470618 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5296334396653926 1101 2 0.7947964311908962 1101 3 0.8323948182297549 1101 4 0.8550308659602095 1101 5 0.688530191395671 1101 6 0.9440932331519036 1101 7 0.930699577600933 1101 8 0.7496297841891675 1101 9 0.7756790165164844 1101 10 0.7700899043038802 1101 11 0.9244197239261425 1101 12 1.1949959687815763 1101 13 0.9613730125936684 1101 14 0.8840256787412528 1101 15 0.7267031852737915 1101 16 1.1871093710992398 1101 17 1.2403204892707578 1101 18 1.4874477210069443 1101 19 0.8782145114568891 1101 20 1.0933722964161205 1101 21 1.0305378301755184 1101 22 0.9781863188303532 1101 23 1.2964017887928705 1101 24 0.9350569739277361 1101 25 1.1622469020900752 1102 1 0.5651347996667084 1102 2 0.6001923431201384 1102 3 0.6104454621255684 1102 4 0.6709569786903344 1102 5 0.5974262760172735 1102 6 0.6682759596862979 1102 7 0.7981796474923897 1102 8 0.7683446240630118 1102 9 0.8017907676640448 1102 10 0.9995481671831712 1102 11 0.9165747131690907 1102 12 0.9782398754039576 1102 13 0.8880129047647145 1102 14 0.9523345936614831 1102 15 1.0638859658479731 1102 16 1.016236973134582 1102 17 0.9514139961161874 1102 18 1.248808842277711 1102 19 1.2353185897378296 1102 20 1.1437010384560224 1102 21 0.9823713061791111 1102 22 1.3019211416777452 1102 23 1.3629864037437187 1102 24 1.2698752005298815 1102 25 1.1049788639614775 1103 1 0.27681964136704806 1103 2 0.49725553079714047 1103 3 0.48100020217927053 1103 4 0.6329896009482852 1103 5 0.1673094528753296 1103 6 0.602470345205532 1103 7 0.4876485566313207 1103 8 0.6223663423331729 1103 9 0.7968651143736452 1103 10 0.660139044448222 1103 11 0.788718684473757 1103 12 0.8431153372520654 1103 13 0.8500899208796042 1103 14 1.0918689216033002 1103 15 1.0686951473999997 1103 16 1.1294907113286783 1103 17 1.0140599992332398 1103 18 1.2402785684012656 1103 19 1.2765085944131371 1103 20 1.1387514101886111 1103 21 0.9555834946291597 1103 22 1.1866408105309318 1103 23 1.4424870451221494 1103 24 1.2830979165567555 1103 25 1.2031036269122382 1104 1 0.5096559315859572 1104 2 0.5333244002395574 1104 3 0.5322493500063104 1104 4 0.8217255515722854 1104 5 0.568043736444757 1104 6 0.5771763150823688 1104 7 0.8022496010140259 1104 8 0.8080297104955037 1104 9 0.860403317770043 1104 10 1.0416057676328379 1104 11 0.9536484736670587 1104 12 0.901637610307958 1104 13 0.9794241222206708 1104 14 1.100017587790994 1104 15 1.2015494648951872 1104 16 1.012520635482943 1104 17 1.1199557056307512 1104 18 1.3506964601544027 1104 19 1.221285387874147 1104 20 1.2020220903041547 1104 21 1.2552152284820934 1104 22 1.1574853078453913 1104 23 1.420994746442041 1104 24 1.274134574891896 1104 25 1.0313412376572444 1105 1 0.5569667903618196 1105 2 0.6237612104127876 1105 3 0.624885344492327 1105 4 0.7592815425463044 1105 5 0.42518629721656964 1105 6 0.3271914114826089 1105 7 0.6771480846686089 1105 8 0.7151921836970772 1105 9 0.6523081278350986 1105 10 0.8603624614507979 1105 11 0.8014718691234677 1105 12 0.8001794647534624 1105 13 0.7949265507961698 1105 14 0.8519601703460751 1105 15 1.0874232352794948 1105 16 0.9380578172684224 1105 17 0.9715639445976905 1105 18 0.8621689093165799 1105 19 0.9701644199559425 1105 20 0.9438575310208677 1105 21 0.9113036344837582 1105 22 0.5903385941742769 1105 23 0.8188718776740274 1105 24 0.8617182580356868 1105 25 0.9193677361926191 1106 1 0.5806952502616696 1106 2 0.7118196624667057 1106 3 0.6895550661270562 1106 4 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1 -1.5491533407380516 2214 2 -3.6403057199577873 2214 3 -1.7575557879807135 2214 4 -1.4176635529716215 2214 5 -1.434321808469175 2214 6 -1.5851328079420135 2214 7 -1.6886506237524799 2214 8 -1.3526786382506018 2214 9 -1.6569337859360047 2214 10 -1.6276082775254679 2214 11 -1.3011538491677612 2214 12 -1.8867662652430042 2214 13 -1.7736749221131518 2214 14 -2.846549783480789 2214 15 -3.1357123799134214 2214 16 -2.3618649705472805 2214 17 -2.7112356608396695 2214 18 -2.1780996500331824 2214 19 -4.416757643101441 2214 20 -2.629522247228884 2214 21 -2.595605399527109 2214 22 -2.6251694178221143 2214 23 -2.7835983340772543 2214 24 -2.9154821187378346 2214 25 -2.7703909351500187 2215 1 -0.9060027334462575 2215 2 -2.671760653124597 2215 3 -1.3491505176292904 2215 4 -1.0005495946751601 2215 5 -0.8694308203572838 2215 6 -1.4532142468360263 2215 7 -1.9232667342526106 2215 8 -1.962097774461359 2215 9 -1.7065367715813622 2215 10 -3.17414548727513 2215 11 -2.203057282880252 2215 12 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-3.5796042505131886 2216 24 -3.504495785117676 2216 25 -3.5472066707446075 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 160498.0 3 4.0 4 1297.0 5 7544.0 6 9230.0 7 8325.0 8 7532.0 9 7226.0 10 6718.0 11 6408.0 12 6139.0 13 6045.0 14 6180.0 15 6302.0 16 6511.0 17 6757.0 18 7677.0 19 9326.0 20 11954.0 21 15311.0 22 19145.0 23 23225.0 24 26716.0 25 30611.0 26 34366.0 27 38078.0 28 43628.0 29 52748.0 30 63698.0 31 80000.0 32 100398.0 33 128078.0 34 166834.0 35 220721.0 36 303448.0 37 479096.0 38 1264332.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.461552884538115 18.339098183402587 12.589515889498212 25.609833042561082 2 15.009539270019637 19.545283423564058 41.12601656044569 24.31916074597062 3 19.09750074756862 23.944652629780286 29.111924075506806 27.845922547144287 4 12.516950055073773 16.42331663073695 37.67823329624173 33.38150001794755 5 13.57785231434866 37.5830452177762 35.53454212321635 13.304560344658793 6 32.83873332886456 36.34804330120367 17.7804484945482 13.032774875383577 7 28.74646417280552 31.052637667025845 21.767378236596187 18.433519923572447 8 25.431816941414326 36.76554339288638 20.16586302878147 17.63677663691783 9 26.579155462302023 15.102267448999749 19.80421503600524 38.51436205269299 10 15.20053436679048 28.571064168802486 34.28510497062555 21.943296493781492 11 34.592379520770315 22.044500153344483 24.462152387042657 18.90096793884254 12 23.842300242920743 24.32298077693113 31.25906503691268 20.575653943235444 13 29.204398668238678 21.606626179936082 25.48979304310428 23.699182108720958 14 21.05981468979109 21.915145057623192 27.016544791606105 30.00849546097961 15 23.274010669299187 30.715051875195776 22.788155321835042 23.22278213366999 16 22.50200062999824 27.638473553421417 27.521194220389045 22.3383315961913 17 22.118136856798614 28.400968387407165 27.494454789703198 21.986439966091016 18 22.162575230024558 27.038365531229662 29.290367706784597 21.508691531961183 19 23.942881567437713 26.66413748242153 27.00148399242906 22.391496957711706 20 23.998562022565093 27.06467517074499 27.41856355308563 21.518199253604287 21 23.143615568134393 26.55989387978599 26.77221736253182 23.524273189547802 22 23.610364240954336 27.775274753468583 26.48619581269574 22.128165192881337 23 22.53799984872731 27.52178252622431 27.28953445087271 22.650683174175676 24 23.47395031112274 26.650163621581974 27.717937926127224 22.157948141168067 25 23.04384142033317 26.96117136672304 27.38699131334428 22.607995899599512 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 217.0 1 217.0 2 438.0 3 659.0 4 659.0 5 659.0 6 1235.5 7 1812.0 8 1812.0 9 1812.0 10 2923.0 11 4034.0 12 4034.0 13 4034.0 14 7426.0 15 10818.0 16 10818.0 17 10818.0 18 21120.0 19 31422.0 20 31422.0 21 31422.0 22 51895.5 23 72369.0 24 72369.0 25 72369.0 26 106542.0 27 140715.0 28 140715.0 29 140715.0 30 186758.0 31 232801.0 32 232801.0 33 232801.0 34 269200.0 35 305599.0 36 305599.0 37 305599.0 38 334351.5 39 363104.0 40 363104.0 41 363104.0 42 388395.0 43 413686.0 44 413686.0 45 413686.0 46 434975.5 47 456265.0 48 456265.0 49 456265.0 50 441577.5 51 426890.0 52 426890.0 53 426890.0 54 384128.0 55 341366.0 56 341366.0 57 341366.0 58 295884.0 59 250402.0 60 250402.0 61 250402.0 62 206044.0 63 161686.0 64 161686.0 65 161686.0 66 126044.0 67 90402.0 68 90402.0 69 90402.0 70 66122.0 71 41842.0 72 41842.0 73 41842.0 74 29314.5 75 16787.0 76 16787.0 77 16787.0 78 11523.5 79 6260.0 80 6260.0 81 6260.0 82 4177.0 83 2094.0 84 2094.0 85 2094.0 86 1357.5 87 621.0 88 621.0 89 621.0 90 404.5 91 188.0 92 188.0 93 188.0 94 116.0 95 44.0 96 44.0 97 44.0 98 33.5 99 23.0 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3584703446451565 2 0.03864054095571136 3 0.03493365866909285 4 0.034785383377628105 5 0.04009363881206581 6 0.01660683264405093 7 0.016221316886242603 8 0.022834394885570028 9 0.01933509800700215 10 0.01894958224919383 11 0.01814889567528423 12 0.018860617074314984 13 0.018712341782850243 14 0.026511622113895593 15 0.027816444678785305 16 0.01980957893968932 17 0.021588882437266206 18 0.11179956976441428 19 0.023575771342893726 20 0.020877161038235455 21 0.02476197367461165 22 0.018653031666264346 23 0.02108474644628609 24 0.01800062038381949 25 0.021588882437266206 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3372106.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.54379961913791 #Duplication Level Percentage of deduplicated Percentage of total 1 77.8767319105366 50.26460179426998 2 13.764972300439535 17.768872278451063 3 4.267305042343943 8.262842447003528 4 1.6981721994164065 4.38425944631693 5 0.8330253780603718 2.688331153959262 6 0.4619056136560909 1.78878860224642 7 0.25555029369117505 1.1545930864030531 8 0.17821403935793778 0.9202088996508715 9 0.12262054979057938 0.7122956575384511 >10 0.497223893857468 5.4150327827992495 >50 0.02472725601072255 1.1034563811236335 >100 0.015866032553301462 2.076787915687202 >500 0.001796676517566972 0.8368759078314778 >1k 0.001704539254199015 1.9225696323745611 >5k 1.8427451396746104E-4 0.7004840143443952 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6598 0.1956640746168715 No Hit CTGTAGGACGTGGAATATGGCAAGA 6050 0.17941310267233593 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5769 0.17108003129201751 No Hit CTTTAGGACGTGAAATATGGCGAGG 5141 0.15245665468404612 No Hit GTACATGGGAAGCAGTGGTATCAAC 4615 0.13685809402195542 No Hit CCCATGTACTCTGCGTTGATACCAC 3914 0.11606989815859882 No Hit GTCCTACAGTGTGCATTTCTCATTT 3685 0.1092788898095137 No Hit GTATCAACGCAGAGTACTTTTTTTT 3663 0.10862647852706883 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 2.9655058292948085E-5 9 0.0 0.0 0.0 2.9655058292948085E-5 2.9655058292948085E-5 10 0.0 0.0 0.0 2.9655058292948085E-5 2.9655058292948085E-5 11 0.0 0.0 0.0 8.896517487884426E-5 2.9655058292948085E-5 12 0.0 0.0 0.0 8.896517487884426E-5 2.9655058292948085E-5 13 0.0 0.0 0.0 8.896517487884426E-5 2.9655058292948085E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTACA 65 5.4681834E-5 13.150764 2 TAGGACC 1150 0.0 13.131705 4 CCGTGTA 120 7.4942363E-10 12.659192 9 TGTACCG 160 0.0 12.465274 5 TCTATAC 320 0.0 11.872217 3 GTAGGAC 3425 0.0 11.314137 3 AGGACCT 2310 0.0 11.265258 5 TGTAGGA 3535 0.0 11.230749 2 GTCCTAA 1770 0.0 11.091098 1 CTGTAGG 3505 0.0 11.011527 1 ATACCGT 95 1.370778E-5 10.993181 6 AGGACGT 3345 0.0 10.675648 5 GGACCTG 2350 0.0 10.504125 6 GACGTGG 1700 0.0 10.499372 7 GGACGTG 3265 0.0 10.497318 6 GTCCTAC 3125 0.0 10.185374 1 TAGGACG 3580 0.0 10.107967 4 GCCTTAC 200 2.3646862E-11 10.006216 1 TGGCAAG 2670 0.0 9.965388 18 TTAGGAC 2095 0.0 9.74712 3 >>END_MODULE