FastQCFastQC Report
Thu 26 May 2016
SRR1512981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512981_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3372106
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT61430.1821710230935801No Hit
CTGTAGGACGTGGAATATGGCAAGA60720.18006551395478076No Hit
CTTTAGGACGTGAAATATGGCGAGG55190.16366626671878048No Hit
GTCCTAAAGTGTGTATTTCTCATTT53040.15729042918579667No Hit
GTACATGGGAAGCAGTGGTATCAAC44730.1326470757443568No Hit
CCCATGTACTCTGCGTTGATACCAC44590.1322319049282555No Hit
GTATCAACGCAGAGTACTTTTTTTT42950.12736847536821203No Hit
GTCCTACAGTGTGCATTTCTCATTT36170.10726234584559323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11050.012.398644
GGCGAGG13500.011.59971119
CGTGCGC1251.8313585E-811.40131910
GGTCGAA600.005911409411.07491511
ACCGCCC1053.4524219E-610.8616238
ATATCGA803.7310206E-410.7004916
TGTAGGA34700.010.4380852
GCGTGCG1106.0339353E-610.3660659
TGGCGAG31150.010.29806618
CTGTAGG34600.010.1388451
GGACGTG33850.010.1156786
GTAGGAC34500.010.1132713
AGGACGT34600.010.0916875
AATCCCG1804.0199666E-1010.01793319
TAGACTA2105.456968E-129.9672935
AGGCGTG2105.456968E-129.9566367
AGGACCT22850.09.86815555
ACACCGT1356.343125E-79.8638276
GACGTGA19200.09.8505647
GTCCTAC32750.09.8116861
Produced by FastQC (version 0.11.5)