##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512981_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3372106 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.45438488588437 31.0 31.0 34.0 27.0 34.0 2 30.614167526169105 31.0 31.0 34.0 27.0 34.0 3 30.732577208427017 31.0 31.0 34.0 27.0 34.0 4 34.21599291362727 37.0 35.0 37.0 30.0 37.0 5 33.856244435969685 37.0 35.0 37.0 30.0 37.0 6 33.72251406094589 37.0 35.0 37.0 29.0 37.0 7 33.605569042017066 37.0 35.0 37.0 28.0 37.0 8 33.49394769915299 37.0 35.0 37.0 28.0 37.0 9 34.957966030723824 39.0 35.0 39.0 28.0 39.0 10 34.846979602657804 39.0 35.0 39.0 27.0 39.0 11 34.87777311863862 39.0 35.0 39.0 27.0 39.0 12 34.72508040969056 39.0 35.0 39.0 27.0 39.0 13 34.70106515038377 39.0 35.0 39.0 27.0 39.0 14 35.76962052794307 40.0 36.0 41.0 27.0 41.0 15 35.78195614254119 40.0 36.0 41.0 27.0 41.0 16 35.756100786867314 40.0 36.0 41.0 27.0 41.0 17 35.73364894223373 40.0 36.0 41.0 27.0 41.0 18 35.657499793897344 40.0 36.0 41.0 27.0 41.0 19 35.63203677464469 40.0 36.0 41.0 27.0 41.0 20 35.60385231069249 40.0 36.0 41.0 27.0 41.0 21 35.553585504133025 40.0 36.0 41.0 27.0 41.0 22 35.50984577590384 40.0 36.0 41.0 26.0 41.0 23 35.402434561665615 40.0 36.0 41.0 26.0 41.0 24 35.38757144644919 40.0 36.0 41.0 26.0 41.0 25 35.293034382667685 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.550778554072128 1101 2 0.6205074324838264 1101 3 0.5785560500850693 1101 4 0.33620054995210324 1101 5 0.6115340079294285 1101 6 0.7320513233531685 1101 7 0.7763417609310537 1101 8 0.707919514056627 1101 9 0.5167297525314751 1101 10 0.8737066738156472 1101 11 0.9285444651706243 1101 12 0.9030360946275238 1101 13 0.9277649779441859 1101 14 0.9413687848161345 1101 15 0.8568001905487606 1101 16 1.0532839236776184 1101 17 1.0341669031208838 1101 18 0.5202365199823831 1101 19 0.8655074438181529 1101 20 0.7709382158414257 1101 21 0.9746724232557398 1101 22 0.8809032907211787 1101 23 0.84325322693131 1101 24 0.7767299320697134 1101 25 0.8269707114654921 1102 1 0.5292985217735655 1102 2 0.4680657739165994 1102 3 0.46734001389564384 1102 4 0.3642827953757575 1102 5 0.5253601266900887 1102 6 0.5596420025079851 1102 7 0.6027014685523113 1102 8 0.5289844189740123 1102 9 0.6944596428777032 1102 10 0.712168670139377 1102 11 0.6072202475923376 1102 12 0.7205413439271595 1102 13 0.7971777142605632 1102 14 0.8098814062392279 1102 15 0.8791306054725183 1102 16 0.845718901674033 1102 17 0.8506297719130629 1102 18 0.908593569472643 1102 19 0.9544380982399758 1102 20 0.8854756235136634 1102 21 0.9728590359557145 1102 22 0.9325954017737246 1102 23 0.9719434982815258 1102 24 0.9517747890091712 1102 25 1.0381946578266934 1103 1 0.5032979842293415 1103 2 0.4116858814965667 1103 3 0.4750554444546822 1103 4 0.5229938164891479 1103 5 0.556435979502389 1103 6 0.5572447724705327 1103 7 0.5104161228880102 1103 8 0.5656642983894358 1103 9 0.6104845136852717 1103 10 0.7216658582798914 1103 11 0.7065691491617514 1103 12 0.7203680893485043 1103 13 0.7727368120626821 1103 14 0.805388411425433 1103 15 0.667926353083999 1103 16 0.7621480586568623 1103 17 0.8758520977634348 1103 18 0.8837735796797901 1103 19 0.9063329377963356 1103 20 0.9006413833309637 1103 21 0.5360354519811423 1103 22 0.7549648736057435 1103 23 0.8393726202004785 1103 24 0.8470537718981106 1103 25 0.8244479489849752 1104 1 0.4889756692722109 1104 2 0.5716965104686125 1104 3 0.5433462150514075 1104 4 0.5080069659067874 1104 5 0.5360693924030926 1104 6 0.6261074687139256 1104 7 0.615681511754353 1104 8 0.5371655357501979 1104 9 0.6858006750072789 1104 10 0.7697080248661692 1104 11 0.8130238885176979 1104 12 0.7204673330649101 1104 13 0.7949332988800961 1104 14 0.894634335330835 1104 15 0.8933691363455409 1104 16 1.0460911149510608 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-1.8815891118040042 2216 18 -1.8956460964241586 2216 19 -1.9071988299083387 2216 20 -2.201580051975796 2216 21 -1.97644387311815 2216 22 -2.3360623914278307 2216 23 -1.4470419838845388 2216 24 -1.7001666520380923 2216 25 -1.5836553247342238 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 85312.0 3 2.0 4 1510.0 5 12195.0 6 14093.0 7 10998.0 8 8855.0 9 7909.0 10 6811.0 11 6195.0 12 5809.0 13 5361.0 14 5303.0 15 5289.0 16 5420.0 17 5835.0 18 6262.0 19 7436.0 20 9633.0 21 12221.0 22 15581.0 23 19160.0 24 22427.0 25 26279.0 26 29800.0 27 33307.0 28 37925.0 29 45538.0 30 57601.0 31 73168.0 32 93865.0 33 121151.0 34 157880.0 35 207241.0 36 286052.0 37 447901.0 38 1474781.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.726799861577646 19.072160664902157 13.524196310438837 27.676843163081355 2 13.721729816162254 19.84410919710661 39.947581687872024 26.48657929885911 3 17.185373176288053 23.91870243699338 29.76383304676662 29.13209133995195 4 11.68430832098358 16.818047525086115 36.981799887754704 34.515844266175606 5 13.090054757413855 37.728131161396085 35.784438828502346 13.397375252687718 6 32.78625907754971 36.738430344042435 17.467180738395918 13.008129840011943 7 28.492493825197467 31.12711579319588 21.79976730650456 18.580623075102093 8 25.391473256968478 37.09041823023524 19.870965904066573 17.647142608729713 9 26.214195967786196 14.966630619725537 19.684270331290673 39.13490308119759 10 14.850517235953761 28.756217192195177 34.39157460244704 22.001690969404024 11 34.534643540822394 21.95599921114714 24.47270672912246 19.036650518908 12 23.5619190487503 24.337721877740613 31.32920074039077 20.77115833311831 13 29.076292858144814 21.468478190919424 25.53998897379482 23.91523997714094 14 20.897848885177194 21.82586450592332 26.98176057244435 30.294526036455128 15 23.113113261544576 30.765733570981602 22.76811796211665 23.35303520535717 16 22.429943963475562 27.760161421256864 27.3736034246553 22.436291190612273 17 22.080007356452764 28.477303266798966 27.403646783480017 22.039042593268253 18 22.115087492044967 27.173066577937995 29.10392345610036 21.607922473916677 19 23.830021577519524 26.69016983251622 27.02403420137674 22.45577438858752 20 23.90048923821248 27.021091780875135 27.423348379897018 21.655070601015368 21 22.981873911843092 26.481650460647533 26.807975205962098 23.72850042154728 22 23.460083307187396 27.71147220513757 26.524318095144327 22.304126392530705 23 22.301879003803556 27.46645568081193 27.350326472536747 22.88133884284776 24 23.44178978952619 26.70346068599267 27.711287842078512 22.14346168240263 25 22.949176930568456 26.946968160255658 27.36621340612074 22.73764150305515 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 104.0 1 104.0 2 275.0 3 446.0 4 446.0 5 446.0 6 1089.0 7 1732.0 8 1732.0 9 1732.0 10 2767.5 11 3803.0 12 3803.0 13 3803.0 14 7218.5 15 10634.0 16 10634.0 17 10634.0 18 21003.0 19 31372.0 20 31372.0 21 31372.0 22 51985.0 23 72598.0 24 72598.0 25 72598.0 26 107202.5 27 141807.0 28 141807.0 29 141807.0 30 186972.5 31 232138.0 32 232138.0 33 232138.0 34 269099.5 35 306061.0 36 306061.0 37 306061.0 38 335501.5 39 364942.0 40 364942.0 41 364942.0 42 389752.5 43 414563.0 44 414563.0 45 414563.0 46 436757.5 47 458952.0 48 458952.0 49 458952.0 50 444251.0 51 429550.0 52 429550.0 53 429550.0 54 385150.5 55 340751.0 56 340751.0 57 340751.0 58 294942.5 59 249134.0 60 249134.0 61 249134.0 62 204566.5 63 159999.0 64 159999.0 65 159999.0 66 123967.0 67 87935.0 68 87935.0 69 87935.0 70 64477.0 71 41019.0 72 41019.0 73 41019.0 74 28551.5 75 16084.0 76 16084.0 77 16084.0 78 11005.5 79 5927.0 80 5927.0 81 5927.0 82 3898.5 83 1870.0 84 1870.0 85 1870.0 86 1210.0 87 550.0 88 550.0 89 550.0 90 332.5 91 115.0 92 115.0 93 115.0 94 65.0 95 15.0 96 15.0 97 15.0 98 10.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08030589785730342 2 5.931011658589617E-5 3 0.0 4 0.027223343512926344 5 0.06921490605574084 6 0.07909004046729255 7 0.034044006920304404 8 0.0635804449800807 9 0.04451224249771508 10 0.10746993125364387 11 0.003588262053446719 12 0.011061336743269637 13 0.004122053102719785 14 0.005337910492730655 15 0.005219290259558864 16 0.016458557352586188 17 0.027164033396340447 18 8.303416322025465E-4 19 0.002313094546849951 20 0.0038255025197903034 21 1.4827529146474043E-4 22 2.3724046634358468E-4 23 0.0 24 0.0 25 0.00278757547953712 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3372106.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.36788915669419 #Duplication Level Percentage of deduplicated Percentage of total 1 76.71432703211953 48.612249721017385 2 14.482958120088071 18.355089696295696 3 4.495812408091801 8.546704270357548 4 1.7932383012935242 4.5453490363162645 5 0.870404891350385 2.757786033826782 6 0.47075168718027666 1.7898324440139928 7 0.2808805539479889 1.2459165466192914 8 0.18647169155501028 0.9453053985055326 9 0.12783773217239036 0.7290726518108872 >10 0.5320987740292294 5.6915119871076305 >50 0.024574428026164278 1.0689443772413967 >100 0.016941729388426578 2.1903650081781016 >500 0.0015935458993885927 0.7115797618599069 >1k 0.0019216288704840938 2.1260669096353677 >5k 1.8747598736430177E-4 0.6842261572142153 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6143 0.1821710230935801 No Hit CTGTAGGACGTGGAATATGGCAAGA 6072 0.18006551395478076 No Hit CTTTAGGACGTGAAATATGGCGAGG 5519 0.16366626671878048 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5304 0.15729042918579667 No Hit GTACATGGGAAGCAGTGGTATCAAC 4473 0.1326470757443568 No Hit CCCATGTACTCTGCGTTGATACCAC 4459 0.1322319049282555 No Hit GTATCAACGCAGAGTACTTTTTTTT 4295 0.12736847536821203 No Hit GTCCTACAGTGTGCATTTCTCATTT 3617 0.10726234584559323 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.9655058292948085E-5 0.0 6 0.0 0.0 0.0 2.9655058292948085E-5 2.9655058292948085E-5 7 0.0 0.0 0.0 2.9655058292948085E-5 2.9655058292948085E-5 8 0.0 0.0 0.0 2.9655058292948085E-5 2.9655058292948085E-5 9 0.0 0.0 0.0 5.931011658589617E-5 2.9655058292948085E-5 10 0.0 0.0 0.0 5.931011658589617E-5 2.9655058292948085E-5 11 0.0 0.0 0.0 8.896517487884426E-5 2.9655058292948085E-5 12 0.0 0.0 0.0 1.1862023317179234E-4 2.9655058292948085E-5 13 0.0 0.0 0.0 1.1862023317179234E-4 2.9655058292948085E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 1105 0.0 12.39864 4 GGCGAGG 1350 0.0 11.599711 19 CGTGCGC 125 1.8313585E-8 11.401319 10 GGTCGAA 60 0.0059114094 11.074915 11 ACCGCCC 105 3.4524219E-6 10.861623 8 ATATCGA 80 3.7310206E-4 10.700491 6 TGTAGGA 3470 0.0 10.438085 2 GCGTGCG 110 6.0339353E-6 10.366065 9 TGGCGAG 3115 0.0 10.298066 18 CTGTAGG 3460 0.0 10.138845 1 GGACGTG 3385 0.0 10.115678 6 GTAGGAC 3450 0.0 10.113271 3 AGGACGT 3460 0.0 10.091687 5 AATCCCG 180 4.0199666E-10 10.017933 19 TAGACTA 210 5.456968E-12 9.967293 5 AGGCGTG 210 5.456968E-12 9.956636 7 AGGACCT 2285 0.0 9.8681555 5 ACACCGT 135 6.343125E-7 9.863827 6 GACGTGA 1920 0.0 9.850564 7 GTCCTAC 3275 0.0 9.811686 1 >>END_MODULE